The receptor binding domain of SARS-CoV-2 spike protein is the result of an ancestral recombination between the bat-CoV RaTG13 and the pangolin-CoV MP789Research Note Published on 2020-08-272022-10-27 Journal: BMC Research Notes [Category] COVID-19, [키워드] bat-CoV conserved coronavirus CoV COVID-19 pandemic CoVs genealogy genetic analysis glycoprotein Haplotype Host Human humans identity molecular evolution natural selection Novel coronavirus nucleotide objective pangolin CoV pangolin CoVs pangolin-CoV parental performed Phylogenetic tree Protein question raised RaTG13 RBD Receptor binding domain Recombination recombination event Result S gene S protein SARS-CoV-2 SARS-CoV-2 spike protein Sequence analysis spike glycoprotein the receptor-binding domain the S protein the SARS-CoV-2 the Spike Wuhan, China [DOI] 10.1186/s13104-020-05242-8 PMC 바로가기 [Article Type] Research Note
The receptor binding domain of SARS-CoV-2 spike protein is the result of an ancestral recombination between the bat-CoV RaTG13 and the pangolin-CoV MP789Research Note Published on 2020-08-272022-10-27 Journal: BMC Research Notes [Category] Coronavirus, COVID-19, [키워드] bat-CoV conserved coronavirus CoV COVID-19 pandemic CoVs genealogy genetic analysis glycoprotein Haplotype Host Human humans identity molecular evolution natural selection Novel coronavirus nucleotide objective pangolin CoV pangolin CoVs pangolin-CoV parental performed Phylogenetic tree Protein question raised RaTG13 RBD Receptor binding domain Recombination recombination event Result S gene S protein SARS-CoV-2 SARS-CoV-2 spike protein Sequence analysis spike glycoprotein the receptor-binding domain the S protein the SARS-CoV-2 the Spike Wuhan, China [DOI] 10.1186/s13104-020-05242-8 PMC 바로가기 [Article Type] Research Note
Development and Clinical Application of a Rapid and Sensitive Loop-Mediated Isothermal Amplification Test for SARS-CoV-2 InfectionResearch Article Published on 2020-08-262022-10-30 Journal: mSphere [Category] COVID-19, MERS, [키워드] acute respiratory syndrome addition application applied approach Asymptomatic Asymptomatic carriers Asymptomatic COVID-19 asymptomatic patient clinical Clinical diagnosis close contact Cohort collected coronavirus COVID-19 COVID-19 diagnosis COVID-19 patient COVID-19 testing cross-react detect develop development diagnostics domain facilitate feasible hospital hospitals independent isothermal amplification lab less likelihood ratio Nasopharyngeal swab nasopharyngeal swabs Next-generation sequencing offer outbreak Patient patients PCR positive prospective cohort Rapid recruited required respiratory Respiratory pathogens respiratory sample respiratory viral infections RT-LAMP RT-qPCR S gene Sample SARS-CoV-2 SARS-COV-2 infection sensitive sensitivity Sensitivity and specificity sputum samples Test tested Treatment yielding [DOI] 10.1128/mSphere.00808-20 PMC 바로가기 [Article Type] Research Article
Adaptive Evolution of Feline Coronavirus Genes Based on Selection AnalysisResearch Article Published on 2020-08-132022-10-27 Journal: BioMed Research International [Category] Coronavirus, MERS, [키워드] adaptive aligned Analysis analyzed Ascite Bayesian breakpoint China clade Cluster conditions conducted coronavirus country described Evidence Evolution GenBank database Genes genes encoding greater healthy help include investigated Netherland Partial Phylogenetic analysis positive Positive selection Protein protein sequence provide Recombination replicative Result S gene screened selected Selection selection pressure sequence severe pathology survival tested viral genome sequences virulence virus [DOI] 10.1155/2020/9089768 PMC 바로가기 [Article Type] Research Article
SARS-CoV-2 exhibits intra-host genomic plasticity and low-frequency polymorphic quasispeciesArticle Published on 2020-08-112022-11-01 Journal: Journal of Clinical Virology [Category] COVID-19, MERS, SARS, [키워드] affecting alterations analyzed anti-viral drug antiviral agent assays Atypical Bioinformatics analysis changes China Chinese patient circulating clinical sample Coronavirus disease 2019 COVID-19 epidemic death diagnostic diagnostics effective Epidemic exhibit Frequency genomic genomic plasticity Genomic rearrangements genomic region Genomic variability highlight Host identify immunological infected with SARS-CoV-2 Intervention Intra-host variability majority Missense molecular molecular diagnostics NGS Novel coronavirus ORF1ab outbreak plasticity Pneumonia primer probe Quasispecies quasispecy Recombination Region reported S gene SARS-CoV-2 sensitivity single nucleotide variation SNV SNVs spike Spread Structural variation Transmission Vaccines Variation viral genes viral genome virus Wuhan [DOI] 10.1016/j.jcv.2020.104585 PMC 바로가기 [Article Type] Article
Molecular Characterization of Porcine Epidemic Diarrhea Virus and Its New Genetic Classification Based on the Nucleocapsid GeneArticle Published on 2020-07-232022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] age antigenic antigenicity average B-cell epitope Bayesian cause characterization classification COE demonstrated detection rate epidemic diarrhea genetic mutation Host mutant N gene N1 and N2 New nucleocapsid nucleocapsid protein PEDV Phylogenetic analysis Porcine epidemic diarrhea virus S gene sequence significantly lower strain Strains the spike protein virus [DOI] 10.3390/v12080790 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Titers in Wastewater Are Higher than Expected from Clinically Confirmed CasesResearch Article Published on 2020-07-212022-10-29 Journal: mSystems [Category] COVID-19, [키워드] approach Asymptomatic Clinical course clinically collected complementary Confirmed confirmed case COVID-19 prevalence develop effort epidemiological expected Fatality rate greater help IMPROVE infect Infectious disease Laboratory magnitude Massachusett N gene novel coronavirus SARS-CoV-2 outbreak Outbreaks pandemic PCR product PMMoV positive Precision Prevalence progression of disease protocol qPCR analysis quarantine RT-qPCR S gene Sanger sequencing SARS-CoV-2 SARS-CoV-2 RNA Sequencing analysis significantly significantly higher social distancing Stool Surveillance tested the SARS-CoV-2 titer Treatment viral titer viral titers wastewater [DOI] 10.1128/mSystems.00614-20 PMC 바로가기 [Article Type] Research Article
Rapid and sensitive detection of SARS-CoV-2 RNA using the Simplexa™ COVID-19 direct assayResearch article Published on 2020-07-012022-10-05 Journal: Journal of Clinical Virology [Category] SARS, 치료기술, [키워드] 95% CI acute respiratory syndrome amplification Analysis assays clinical clinical specificity COVID-19 Diagnosis Direct evaluate faster FIVE infected patients Isolation Laboratory limit of detection Molecular assay Nasopharyngeal swab objective ORF1ab performed Rapid rapid response real-time RT-PCR required Result S gene SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 RNA detection Sensitivity and specificity significantly specimens Surveillance target gene tested viral nucleic acid Viral RNA were assessed [DOI] 10.1016/j.jcv.2020.104416 [Article Type] Research article
The four of structural genes sequences of a porcine epidemic diarrhea virus from domestic piglet in Fujian, ChinaShort Report Published on 2020-06-182022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] Analysis analyzed China Chinese COE conserved determine E gene epidemic diarrhea Genetic characterization homologous homology Membrane gene Mutation Nucleocapsid gene pandemic PEDV Phylogenetic analysis Phylogenetic relationship Porcine epidemic diarrhea virus prevalent Recombination S gene sequence Small membrane gene spike gene strain Strains the vaccine vaccination variant virulent virus [DOI] 10.1186/s12985-020-01345-7 PMC 바로가기 [Article Type] Short Report
A Novel Synonymous Mutation of SARS-CoV-2: Is This Possible to Affect Their Antigenicity and Immunogenicity?Brief Report Published on 2020-05-142022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] analyzed antigenic antigenicity B-cell epitope Characteristics coronavirus COVID-19 D614G effective evade Evolution of SARS-CoV-2 exhibited host immune system Mutation novel Pathogenesis Phylogenetic analysis Prevent reduced required S gene S glycoprotein S1 domain SARS-CoV-2 SARS-CoV-2 strains sequence serological Spike protein strain subtype Subtypes synonymous Vaccine viral entry virus [DOI] 10.3390/vaccines8020220 PMC 바로가기 [Article Type] Brief Report