Inhibition of Autophagy Suppresses SARS-CoV-2 Replication and Ameliorates Pneumonia in hACE2 Transgenic Mice and Xenografted Human Lung Tissues자가포식 억제는 SARS-CoV-2 복제를 억제하고 hACE2 형질전환 마우스 및 이종이식 인간 폐 조직에서 폐렴을 개선합니다Article Published on 2021-11-232022-09-11 Journal: Journal of Virology [Category] SARS, 유전자 메커니즘, [키워드] 3-MA accompanied Activation acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 angiotensin animal model animal models anti-SARS-CoV-2 therapeutics Atg13 ATG14 Atg5 autophagic autophagy autophagy-inhibiting agent autophagy-inhibiting agents block caused Combination complex complexes contribute coronavirus Crab-eating macaque Critical elements enzyme events experimental evidence global pandemic hACE2 Huh-7 cells Human human Angiotensin-converting enzyme Human angiotensin-converting enzyme 2 human lung human lung tissue IMPROVE in vitro in vivo induce Infection inhibit inhibition insight interfere involved mechanism mice modulation mouse mTOR pathway Pneumonia potential therapeutic target potential therapeutic targets SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 replication severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 suppresse therapeutic agent therapeutic agents thought tissues transgenic mice Transgenic mouse Vero E6 virus infection Virus-host interaction Virus-host interactions Vps34 [DOI] 10.1128/JVI.01537-21 PMC 바로가기 [Article Type] Article
SARS-CoV-2 receptor binding domain radio-probe: a non-invasive approach for angiotensin-converting enzyme 2 mapping in mice Research Published on 2021-11-232022-10-05 Journal: Acta pharmacologica Sinica [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] ACE2 ACE2 expression angiotensin-converting enzyme 2 approach BALB/c mice bind binding binding domain biodistribution blocked C-terminus Cell COVID-19 distribution expression profile hACE2 high uptake in vivo Infection Inflammation inhibiting injection interact intrapulmonary Intravenous injection low-dose lung mammals metabolism mice moderate molecular Non-invasive organ pathway probe purification Radiation Radiotherapy RBD Receptor binding domain Recombinant spike protein reduce respiratory epithelial cells SARS-CoV-2 SARS-CoV-2 receptor Spike protein was obtained [DOI] 10.1038/s41401-021-00809-y [Article Type] Research
SARS-CoV-2 B.1.1.7 (alpha) and B.1.351 (beta) variants induce pathogenic patterns in K18-hACE2 transgenic mice distinct from early strainsSARS-CoV-2 B.1.1.7(알파) 및 B.1.351(베타) 변이체는 초기 균주와 구별되는 K18-hACE2 형질전환 마우스에서 병원성 패턴을 유도합니다Article Published on 2021-11-122022-09-10 Journal: Nature Communications [Category] MERS, SARS, 변종, 진단, 치료기술, [키워드] (alpha (Beta Adaptive immunity Alpha Antibody neutralization Antimicrobial responses B.1.1.7 B.1.351 caused combating COVID-19 cytokine D-dimer death hACE2 help hypoxia Immunity immunization induce Infection intramuscular K18-hACE2 K18-hACE2 mice K18-hACE2 transgenic mice lesion less medical intervention mice Mutant SARS-CoV-2 neutralization titer organ pathogenic pathology Receptor binding domain reduced Reinfection resulting SARS-CoV-2 SARS-CoV-2 B.1.1.7 SARS-CoV-2 strain SARS-CoV-2 strains SARS-CoV-2 variant SARS-CoV-2 variants Signaling strain Strains Transgenic mouse Transmissibility variant variants variants of concern Viral viral infection viral spike virulence VoC VOCs [DOI] 10.1038/s41467-021-26803-w PMC 바로가기 [Article Type] Article
Rapid Assessment of Binding Affinity of SARS-COV-2 Spike Protein to the Human Angiotensin-Converting Enzyme 2 Receptor and to Neutralizing Biomolecules Based on Computer SimulationsImmunology Published on 2021-11-112022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, SARS, [키워드] Affect affinity antibody approach assessment bind binding affinities binding affinity Biomolecule can be used cause change circulating computer Coronavirus disease 2019 COVID-19 domain Dynamics Effect experimental work expression facilitate hACE2 hACE2 receptor helping host cell membrane hotspot Human human ACE2 human Angiotensin-converting enzyme infect Infection modulate molecular dynamics mutated Mutation mutations Neutralizing Neutralizing antibodies protein-protein interaction protocol Rapid S-RBD S1 domain S2 domain SARS-CoV-2 SARS-CoV-2 genome Screening selected Simulation spike spike glycoprotein Spike protein Spike-RBD the binding affinity time-consuming vaccination variant virus [DOI] 10.3389/fimmu.2021.730099 PMC 바로가기 [Article Type] Immunology
Enhanced protective immunity against SARS-CoV-2 elicited by a VSV vector expressing a chimeric spike protein키메라 스파이크 단백질을 발현하는 VSV 벡터에 의해 유발되는 SARS-CoV-2에 대한 보호 면역 강화Article Published on 2021-11-102022-09-12 Journal: Signal Transduction and Targeted Therapy [Category] MERS, SARS, 변종, 유전자 메커니즘, 진단, [키워드] ACE2 administration Antigen cellular receptor Chimera chimeric coronavirus COVID-19 vaccines cross-neutralizing cross-reactivity dose elicit elicited enhanced expressing Genetic glycoprotein hACE2 Human human ACE2 humoral immune response immunization immunized immunized mice indicate intramuscular intranasal lesion Lungs macaque macaques mice Mouse models NAb nAb response NAbs neutralizing antibody protective immunity RBD receiving Receptor binding domain replacing rVSV-SARS-CoV-2 S protein Safe SARS-CoV SARS-CoV S SARS-CoV-2 SARS-CoV-2 RBD sera serum significantly significantly increased similarity single dose spike Spike protein spikes the S protein the SARS-CoV-2 titers transplantation Vaccines viruses VSV wild-type [DOI] 10.1038/s41392-021-00797-9 PMC 바로가기 [Article Type] Article
In silico binding profile characterization of SARS-CoV-2 spike protein and its mutants bound to human ACE2 receptorSARS-CoV-2 스파이크 단백질 및 인간 ACE2 수용체에 결합된 돌연변이체의 인 실리코(in silico) 결합 프로필 특성화Article Published on 2021-11-052022-09-11 Journal: Briefings in Bioinformatics [Category] MERS, SARS, 변종, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus affected approach binding binding affinities binding affinity binding free energies binding free energy complexes coronavirus country COVID-19 decomposition domain drug resistance free energies free energy Free energy calculation and decomposition Free energy calculations G476S hACE2 hACE2 protein hotspot Hotspot residues human ACE2 human ACE2 receptor infect interfere investigated MD simulation molecular molecular dynamics Molecular mechanics mutant nine Novel coronavirus pandemic Poisson Protein–protein interaction protocol prototype provide RBD RBD mutations RBDs receptor Receptor-binding domain respiratory SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2 Spike RBD severe acute respiratory syndrome Coronavirus small molecule small molecule drug small molecule drugs spike spike glycoprotein Spike RBD/hACE2. Structure structures surface area V367F V483A Vaccine virus [DOI] 10.1093/bib/bbab188 PMC 바로가기 [Article Type] Article
Comparison of viral RNA–host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2병원성 RNA 바이러스에 걸친 바이러스 RNA-숙주 단백질 상호작용의 비교는 SARS-CoV-2에 대한 신속한 항바이러스 약물 발견을 알려줍니다Article Published on 2021-11-042022-09-11 Journal: Cell Research [Category] MERS, SARS, 변종, 신약개발, 치료제, [키워드] Analysis antiviral drug B.1.351 B.1.351 variant bind broad-spectrum antiviral broad-spectrum antivirals Cell Cell Biology Cepharanthine Comparative Ebola effort engage examined Factor FDA-approved drug hACE2 Host host cell host cells host response IGF2BP1 Infection Interaction pathogen pathogenic Pathogens Perspective promote Protein protein interaction proteomics Research restrict RNA virus RNA viruses SARS-CoV-2 SARS-CoV-2 B.1.351 variant transgenic mice translation Trifluoperazine utility Viral viral infection Viral RNA viral RNAs viruses vRNA Zika [DOI] 10.1038/s41422-021-00581-y PMC 바로가기 [Article Type] Article
Development of an Inactivated Vaccine against SARS CoV-2SARS COV-2에 대한 불활성 백신의 개발Article Published on 2021-11-022022-09-10 Journal: Vaccines [Category] 비임상, [키워드] Adenovirus Aluminium aluminium hydroxide animal models approved AstraZeneca BALB/c mice breath candidate candidate vaccine changed clinical development clinical trial Complete COVID-19 COVID-19 pandemic COVID-19 vaccine develop development Device distribution Efficacy elicited Emergency use ERUCoV-VAC ferrets hACE2 HCoV help human clinical trial human clinical trials hydroxide immunogenic immunogenicity inactivated Inactivated vaccine include Johnson & Johnson K18-hACE2 Medical devices Medicine mice Moderna mRNA name outbreak Pfizer-BioNTech Phase 3 phase 3 clinical trial progression Protective respiratory safety evaluation SARS CoV-2 SARS-CoV-2 SARS-CoV-2 vaccine Sinopharm Sinovac Spread strain strong immune response the vaccine the WHO transgenic mice TURKOVAC university upper respiratory tract upper respiratory tracts Vaccine vaccine candidate Vaccines Viral viral clearance rate Viral vector viral vectors was used [DOI] 10.3390/vaccines9111266 PMC 바로가기 [Article Type] Article
Potent SARS-CoV-2 neutralizing antibodies with protective efficacy against newly emerged mutational variants새로 출현한 돌연변이 변이체에 대한 보호 효능이 있는 강력한 SARS-CoV-2 중화 항체Article Published on 2021-11-022022-09-10 Journal: Nature Communications [Category] MERS, SARS, 변종, 유전자 메커니즘, 진단, [키워드] antibodies antibody authentic SARS-CoV-2 virus B.1.351 bind candidate Candidates COVID-19 COVID-19 pandemic cryo-EM E484K mutation Efficacy evidenced expressing hACE2 human ACE2 Immune escape in vitro in vivo mAbs mechanism monoclonal antibodies monoclonal antibody Mutation Neutralizing Neutralizing antibodies overlapping potent Prophylactic Prophylaxis Protective Protein RBD Receptor binding domain recognize reducing SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 mutants SARS-CoV-2 neutralizing antibody SARS-CoV-2 spike SARS-CoV-2 virus spike steric Structural analysis the RBD the SARS-CoV-2 transgenic mice treat unique variant virus virus infection viruses weight loss [DOI] 10.1038/s41467-021-26539-7 PMC 바로가기 [Article Type] Article
Pharmacological inhibition of fatty acid synthesis blocks SARS-CoV-2 replication지방산 합성의 약리학적 억제는 SARS-CoV-2 복제를 차단합니다Article Published on 2021-11-012022-09-11 Journal: Nature metabolism [Category] MERS, SARS, 변종, 신약개발, 진단, 치료제, [키워드] ACACA acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 airway airways block cellular clinical clinical trials contagious coronavirus COVID-19 death Delta drug Drug administration drug candidate drug candidates Efficacy evaluate FASN fatty Fatty acid food Genes hACE2 humans identify in vitro inflammatory disease inhibit inhibiting inhibitor injection Injections Intervention K18-hACE2 K18-hACE2 transgenic mice leads lung Lung pathology Lungs metabolic genes mouse model Multi-organ damage offer orlistat pathway pharmacological promote reduced replication of SARS-CoV-2 required respiratory RNA SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 replication SARS-CoV-2 variants SARS-CoV-2 viral SARS-CoV-2 viral infection screened Screening severe acute respiratory syndrome Coronavirus severe COVID-19 survival target transgenic mice Treatment treatment of COVID-19 Trial variants Viral [DOI] 10.1038/s42255-021-00479-4 PMC 바로가기 [Article Type] Article