Molecular Dynamics Reveals a DNA-Induced Dynamic Switch Triggering Activation of CRISPR-Cas12a분자 역학은 CRISPR-Cas12a의 활성화를 유발하는 DNA 유도 동적 스위치를 보여줍니다Article Published on 2020-12-282022-09-11 Journal: Journal of Chemical Information and Modeling [Category] SARS, 치료기술, 치료제, [키워드] Activation binding Cas9 catalytic site cleavage conformational coronavirus CRISPR Critical determinant Diagnosis DNA DNA binding domain dynamic Dynamics effort favor Genome IMPROVE induce joint molecular molecular dynamics MOST nonspecific Nuc nucleic acid Nucleic acid detection nucleic acids outcome REC regulator SARS-CoV-2 coronavirus specificity switch tendency the SARS-CoV-2 Viral Viral detection [DOI] 10.1021/acs.jcim.0c00929 PMC 바로가기 [Article Type] Article
Droplet Microarray Based on Nanosensing Probe Patterns for Simultaneous Detection of Multiple HIV Retroviral Nucleic Acids여러 HIV 레트로바이러스 핵산의 동시 검출을 위한 나노센싱 프로브 패턴을 기반으로 하는 액적 마이크로어레이Article Published on 2020-12-162022-09-11 Journal: ACS applied materials & interfaces [Category] SARS, 치료기술, 치료제, [키워드] 2D MoS2 nanosheets acid Adsorption demonstrated dendrimer detection droplet droplet microarray (DMA) energy transfer FIVE fluorescent fluorescent dye fluorescent resonance energy transfer (FRET) HIV HIV nucleic acids HIV-1 HIV-2 Human immunodeficiency virus human immunodeficiency virus-2 hybridized immunodeficiency Influenza influenza A Influenza A) limit of detection M gene Microarray molybdenum Multiple multiplex N gene N genes nucleic acid nucleic acids omniphobic. pattern platform probe proof proof of concept retroviral separated Simultaneous small sample size Viral viral gene viral genes viral infection viral nucleic acid viral nucleic acids weakening [DOI] 10.1021/acsami.0c16146 PMC 바로가기 [Article Type] Article
A sensitive and affordable multiplex RT-qPCR assay for SARS-CoV-2 detectionSARS-CoV-2 검출을 위한 민감하고 저렴한 멀티플렉스 RT-qPCR 분석Controlled Clinical Trial Published on 2020-12-152022-08-13 Journal: PLoS Biology [Category] Biochemistry, MERS, SARS, 임상, 진단, 치료기술, 치료제, [키워드] (coronavirus disease 2019 acute respiratory syndrome coronavirus assays benefit Better can not caused cellular collected coronavirus coronavirus 2 coronavirus disease Coronavirus disease 2019 correlation COVID-19 diagnostic test diagnostic tests Efficiency false-negative rates feasibility help Herpe identify Inclusion infected individuals material MONITOR Multiplex assay multiplex RT-qPCR nose and throat Novel coronavirus novel coronavirus SARS-CoV-2 nucleic acid nucleic acid extraction nucleic acids pandemic PCR tests probe provide Quality control Quantitative reduce reduced respiratory respiratory syndrome coronavirus 2 reverse transcription PCR robust RPP30 RT-qPCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 qRT-PCR severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 statistically significant supply swab sample swab samples swabbing symptomatic target Variability Viral viral nucleic acid Viral RNA viral spike [DOI] 10.1371/journal.pbio.3001030 PMC 바로가기 [Article Type] Controlled Clinical Trial
Disposable silicon-based all-in-one micro-qPCR for apid on-site detection of pathogens병원체의 신속한 현장 검출을 위한 일회용 실리콘 기반 all-in-one micro-qPCRArticle Published on 2020-12-022022-09-10 Journal: Nature Communications [Category] MERS, SARS, 바이오마커, 진단, 치료기술, [키워드] against SARS-CoV agent cDNA Course detect Device Diagnosis diagnostic disease-causing effective Electrical and electronic engineering fragment of Genomic DNA infections Intervention lab Laboratory Mycobacterium Novel coronavirus novel coronavirus SARS-CoV-2 nucleic acid nucleic acids pathogen pathogen-specific Pathogens produced Quantitative Rapid Real-time PCR SARS-CoV-2 Sensors and biosensors Sensors and probes sequence silicon specificity Symptom Treatment [DOI] 10.1038/s41467-020-19911-6 PMC 바로가기 [Article Type] Article
The Latest Achievements in the Construction of Influenza Virus Detection AptasensorsReview Published on 2020-11-302022-10-28 Journal: Viruses [Category] Coronavirus, SARS, [키워드] achievement addition aptamer Aptamers aptasensors cause changes in construction contribute death detection diagnostic disease DNA element example flu Infection Influenza Influenza virus initial nucleic acids pandemic Pathogens Protein Proteins Quantitative RNA selected sensitivity specificity Symptoms virus [DOI] 10.3390/v12121365 PMC 바로가기 [Article Type] Review
Effects of methylprednisolone use on viral genomic nucleic acid negative conversion and CT imaging lesion absorption in COVID-19 patients under 50 years old50세 미만 코로나19 환자에서 메틸프레드니솔론 사용이 바이러스 게놈 핵산 음성 전환 및 CT 영상 병변 흡수에 미치는 영향Comparative Study Published on 2020-11-012022-09-11 Journal: Journal of medical virology [Category] SARS, 유전자 메커니즘, [키워드] alleviate breathing clearance Clinical data coronavirus disease Coronavirus disease 2019 Corticosteroid Corticosteroids corticosteroids therapy COVID-19 COVID-19 patient COVID-19 symptom COVID-19 symptoms Critical CT image lesion diagnosed Disease progression Dyspnea Effect Fever genomic IMPROVE in viral Methylprednisolone Negative conversion non-randomized nucleic acid nucleic acids Oxygenation Patient persistent fever Pneumonia prevent disease progression retrospective separated Symptom Treatment two groups use of corticosteroid Viral Viral pneumonia virus virus nucleic acid virus nucleic acid. [DOI] 10.1002/jmv.26052 PMC 바로가기 [Article Type] Comparative Study
Clinical Applications of Visual Plasmonic Colorimetric SensingReview Published on 2020-10-302022-10-29 Journal: Sensors (Basel, Switzerland) [Category] COVID-19, [키워드] Analysis application Bacteria biochemical biological samples Biomarker clinical clinical diagnostics colorimetric sensor coronavirus 2 diagnostics IMPROVE LSPR naked eye nanomaterials nucleic acids Perspective plasmonics point of care powerful tool Proteins provided respiratory SARS-CoV-2 unique variety virus Visual [DOI] 10.3390/s20216214 PMC 바로가기 [Article Type] Review
Extracellular Vesicles in the Pathogenesis of Viral Infections in HumansReview Published on 2020-10-212022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] alteration amplification antiviral immune responses Cell Cellular responses clinically complex coronavirus Enzymes EVs Exosomes extracellular vesicle extracellular vesicles (EVs) facilitate Herpes virus HIV Human Human immunodeficiency virus immune response in viral inhibiting Intercellular communication mechanisms membrane vesicle modulate modulation MOST neurotropic nucleic acids Pathogenesis pathogenicity pathology Proteins retrovirus signaling molecules Therapeutics viral infection viral pathogenicity viral spread viruses Zika Zika virus ZIKV [DOI] 10.3390/v12101200 PMC 바로가기 [Article Type] Review
The epigenetic implication in coronavirus infection and therapyReview Published on 2020-10-212022-10-31 Journal: Clinical Epigenetics [Category] COVID-19, MERS, SARS, [키워드] age aging alteration cause caused changes chemotherapeutic drugs chromatin chronic disease complications consequence coronavirus 2 coronavirus disease Coronavirus infection Coronaviruses COVID-19 COVID-19 severity develop drugs effective Epigenetic epigenetics Gene Expression Genetic Health status highly pathogenic histone methylation Host identify Infection metabolic factors metabolism Methylation modifications Non-coding RNAs nucleic acids pathogenic Patient phenotypic Prophylaxis regulate Research respiratory RNA virus SARS-CoV-2 Spread Therapeutic approach therapy Vaccine viral infection viral outbreak virus Wuhan, China [DOI] 10.1186/s13148-020-00946-x PMC 바로가기 [Article Type] Review
Dynamics of severe acute respiratory syndrome coronavirus 2 genome variants in the feces during convalescence회복기 동안 대변에서 중증 급성 호흡기 증후군 코로나바이러스 2 게놈 변이의 역학Article Published on 2020-10-202022-09-11 Journal: Journal of genetics and genomics = Yi chuan xue ba [Category] SARS, 변종, 유전자 메커니즘, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 capture change changes in clinical samples collected convalescent convalescent phase coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 demonstrated Dynamics Evolution Fecal sample fecal samples feces Frequency gastrointestinal tract genome variant genomic hybrid Hybrid capture individual level individuals Infection Intra-host variant intra-host variants investigated Mutation nine nucleic acid nucleic acids pandemic patients with COVID-19 population level populations random respiratory SARS-CoV-2 SARS-CoV-2 genome severe acute respiratory syndrome Coronavirus Surveillance the SARS-CoV-2 genome Transmission variant Viral viral genome Viral load virus [DOI] 10.1016/j.jgg.2020.10.002 PMC 바로가기 [Article Type] Article