Heat inactivation of the Middle East respiratory syndrome coronavirusShort Articles Published on 2014-09-242022-10-30 Journal: Influenza and Other Respiratory Viruses [Category] Coronavirus, MERS, [키워드] biosafety contrary culture supernatant dropped heat inactivation Increasing initial Laboratory log measure MERS-CoV Middle East reduce respiratory syndrome coronavirus significant decrease significantly submitted TCID temperature tested titre VERO E6 cells viral infectivity [DOI] 10.1111/irv.12261 PMC 바로가기 [Article Type] Short Articles
Molecular Characterizations of Subcellular Localization Signals in the Nucleocapsid Protein of Porcine Epidemic Diarrhea VirusArticle Published on 2014-03-132022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] Amino acid antibiotic Cell cellular characterization chromosome CHROMOSOME region maintenance 1 confocal microscopy coronavirus Critical cytoplasm epidemic diarrhea eukaryotic fluorescent full-length functional fusion protein GFP Infection inhibited Localization Mutation N protein NES nuclear nuclear export signal nucleocapsid nucleocapsid protein Nucleolus pathway PEDV Porcine epidemic diarrhea virus Protein signal site-directed mutagenesis subcellular localization signals the N protein VERO E6 cells virus virus replication [DOI] 10.3390/v6031253 PMC 바로가기 [Article Type] Article
Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike proteinArticle Published on 2014-02-132022-11-01 Journal: Journal of virological methods [Category] Coronavirus, [키워드] ACE2, angiotensin-converting enzyme 2 acute respiratory syndrome against SARS-CoV Amino acid analyses approach binding Binding motif binding site caused Cell cellular coronavirus spike protein determine Effect H7N9 Influenza life threatening MERS-CoV Middle East motif MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide pathogen peptide Peptide inhibitor Psychological RBD, receptor-binding domain RBM, receptor-binding motif required residues respiratory syndrome coronavirus S, spike Safe SARS-CoV SARS-CoV spike protein SARS-CoV, severe acute respiratory syndrome-coronavirus Spike protein synthetic peptide the spike protein Therapeutic approach vaccine candidate VERO E6 cells [DOI] 10.1016/j.jviromet.2014.01.022 PMC 바로가기 [Article Type] Article
Role of the Spike Glycoprotein of Human Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Virus Entry and Syncytia FormationResearch Article Published on 2013-10-032022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] 293T cell activate Affect antibody bats Betacoronavirus blocked cathepsin L cathepsin L-dependent Cell cell type Cl cleave cleaved co-expressed coronavirus coronavirus spike glycoprotein determined elicit endosome Eptesicus experiment Express Extracellular fluids Formation function glycoprotein host cell host protease host range Human immunoblot inhibitor lentivirus maturation membrane fusion MERS MERS-CoV MERS-CoV S protein MHV Middle East murine Neutral Neutralizing antibodies New World non-infectious plasma membrane polyclonal produced producing pseudotype pseudovirion pseudovirus entry reagent receptor released Research respiratory role S protein S2 domain serine protease Spike protein Syncytia syncytia formation the S protein the Spike tissue Treatment triggered trypsin variety VERO E6 cells viral spike virus virus virus entry [DOI] 10.1371/journal.pone.0076469 PMC 바로가기 [Article Type] Research Article
Ezrin Interacts with the SARS Coronavirus Spike Protein and Restrains Infection at the Entry StageResearch Article Published on 2012-11-212022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] Amino acid amino-acid Cell cellular Cluster complex coronavirus cysteine cytosol dependent on domain dominant negative early stage eight endodomain envelope fusion epithelial cells event ezrin Factor finding found glycoprotein host cell membrane in vitro Infection interact Interaction Lentiviral particle limit linker mechanism membrane fusion molecular mechanisms Particle performed pseudotyped regulate Regulation respiratory RNA S-pseudotyped particle SARS-CoV SARS-CoV S severe spike Stage susceptibility targeting VERO E6 cells viral envelope virus entry Virus life cycle yeast [DOI] 10.1371/journal.pone.0049566 PMC 바로가기 [Article Type] Research Article