Genomic Sequencing and Analysis of Eight Camel-Derived Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Isolates in Saudi ArabiaArticle Published on 2020-06-032022-10-28 Journal: Viruses [Category] COVID-19, [키워드] 2019-nCoV 3D structure Analysis Antibody binding appear B cell betacoronaviruses bioinformatics cause Clustering Complete contribute coronavirus Dromedary camel effective vaccine eight epitope evolutionary relationship exhibited Genome Gulf homology Host Human humans isolate MERS-CoV MERS-CoV genome Middle East Neutralizing antibodies occurred outbreak Phylogenetic analysis predict Region respiratory respiratory syndrome coronavirus SARS-CoV Saudi Arabia selected sequence sequenced severe respiratory illness spike glycoprotein Spike protein strain Strains Vaccine design virus [DOI] 10.3390/v12060611 PMC 바로가기 [Article Type] Article
Protective Adaptive Immunity Against Severe Acute Respiratory Syndrome Coronaviruses 2 (SARS-CoV-2) and Implications for VaccinesPathology Published on 2020-06-012022-10-31 Journal: Cureus [Category] COVID-19, [키워드] acute respiratory syndrome Adaptive immune response Against Asymptomatic characterized coronavirus coronavirus disease COVID-19 died disease dry cough elicited Fever global pandemic immune response imperative infected with SARS-CoV-2 Intervention Mild Neutralizing antibodies Novel coronavirus pandemic Pathogenesis Prevent Protective public health respiratory Respiratory disease responsible SARS-CoV-2 SARS-CoV-2 virus Spread T-cell immunity Therapeutic strategies Vaccine Vaccines [DOI] 10.7759/cureus.8399 PMC 바로가기 [Article Type] Pathology
Rapid and robust bioanalytical assays are critical for SARS-CoV-2 therapeutic and vaccine development and beyondCommentary Published on 2020-05-262022-10-28 Journal: Bioanalysis [Category] COVID-19, MERS, SARS, [키워드] COVID-19 Critical Neutralizing antibodies Rapid robust SARS-CoV-2 serology therapeutic Vaccine development Viral load [DOI] 10.4155/bio-2020-0116 PMC 바로가기 [Article Type] Commentary
Evaluation of SARS‐CoV‐2 neutralizing antibodies using a CPE‐based colorimetric live virus micro‐neutralization assay in human serum samples인간 혈청 샘플에서 CPE 기반 비색 생 바이러스 미세 중화 분석을 사용한 SARS-CoV-2 중화 항체 평가Research Article Published on 2020-05-172022-09-12 Journal: Journal of medical virology [Category] MERS, SARS, 진단, [키워드] Adenovirus antibody concentration borderline China comparable CR3022 cytopathic diagnostic ELISA epidemiological Epidemiology evaluated Human monoclonal antibody human serum Humoral immunity immunology immunosorbent Influenza Italian Live virus microneutralization neutralization Neutralization assay Neutralizing antibodies neutralizing antibody objective optical microscope outbreak pandemic performed positive provided SARS Coronavirus SARS‐CoV‐2 SARS‐CoV‐2 virus sensitivity Serological assay serological assays serum sample serum samples Siena spectrophotometer Strains Subjective tested University of Siena Vaccine Virology virus wild type [DOI] 10.1002/jmv.25986 PMC 바로가기 [Article Type] Research Article
Key residues of the receptor binding motif in the spike protein of SARS-CoV-2 that interact with ACE2 and neutralizing antibodiesArticle Published on 2020-05-152022-11-01 Journal: Cellular and Molecular Immunology [Category] COVID-19, SARS, [키워드] ACE2 against SARS-CoV amino acid residue analyzed angiotensin-converting enzyme 2 antibody antigenic bind binding site block viral entry caused Coronavirus disease 2019 coronavirus SARS-CoV-2 COVID-19 COVID-19 pandemic cross-neutralizing cross-neutralizing antibody develop drug epitope F486 feature host receptor human ACE2 Humoral immunity immunization in vivo experiment indicate induce Infection mechanism neutralize Neutralizing antibodies neutralizing antibody neutralizing monoclonal antibody Prophylactic public health RBD receptor binding motif recognize representing residue SARS-CoV SARS-CoV RBD SARS-CoV-2 SARS-CoV-2 RBD Spike protein Substitution mutation the RBD the receptor-binding domain the SARS-CoV-2 the spike protein Vaccine viral spike protein viruses [DOI] 10.1038/s41423-020-0458-z PMC 바로가기 [Article Type] Article
Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization AssaysProtocol Published on 2020-05-062022-10-28 Journal: Viruses [Category] COVID-19, [키워드] 293T cell 293T-ACE2 ACE2 Affect ALAYT antibody Antibody binding assays ATCC BEI Resource Cell complement coronavirus COVID-19 cytoplasmic tail demonstrated described Express human sera Immunity infect lentiviral Lentiviral particle lentiviral pseudotype luciferase neutralization Neutralization assay Neutralizing activity Neutralizing antibodies NIH Particle Particles plasma Protein protocol pseudotype pseudotyped lentiviral particle pseudotyping reagent receptor Repository retroviral SARS-CoV-2 sera spike the SARS-CoV-2 vesicular stomatitis virus viral infection viral particle virus VSV [DOI] 10.3390/v12050513 PMC 바로가기 [Article Type] Protocol
Convalescent plasma in Covid-19: Possible mechanisms of actionArticle Published on 2020-05-052023-06-14 Journal: Autoimmunity reviews [Category] MERS, SARS, [키워드] 2019-nCoV, 2019 novel coronavirus ACE-2 receptor ACE-2, Angiotensin converting enzyme-2 ADE, Antibody-dependent enhancement BAFF, B cell–activating factor BCR, B-cell receptor convalescent plasma coronavirus COVID-19 COVID-19, Coronavirus disease 2019 CP, convalescent plasma Cytokines DCs, dendritic cells E, Envelope HIV, Human immunodeficiency virus ICU, Intensive care unit IGG, immunoglobulin g IGM, immunoglobulin m Intravenous immunoglobulins IVIG, intravenous immunoglobulin M, membrane MERS-CoV, MERS coronavirus MERS, Middle East respiratory syndrome N, nucleoprotein NAbs, neutralizing antibodies NAT, Nucleic acid test Neutralizing antibodies S1-RBD, Spike1-receptor binding protein SARS-CoV-2 SARS-CoV, SARS coronavirus. SARS, severe acute respiratory syndrome coronavirus [DOI] 10.1016/j.autrev.2020.102554 PMC 바로가기 [Article Type] Article
Effectiveness of convalescent plasma therapy in severe COVID-19 patients중증 코로나19 환자에서 회복기 혈장 요법의 효과530 Published on 2020-04-282022-09-06 Journal: Proceedings of the National Academy of Sciences of [Category] Communicable Disease, COVID-19, SARS, [키워드] Absorption addition Antibody titers antiviral agent Antiviral agents approved C-reactive protein clinical benefit Clinical outcome clinical outcomes clinical symptom Clinical symptoms convalescent plasma Convalescent plasma therapy coronavirus disease Coronavirus disease 2019 COVID-19 criteria disappearance Donor dose Endpoint enrolled feasibility FIVE global health Health IMPROVE laboratory parameter Laboratory parameters lung lesion lung lesions lymphocyte Lymphocyte count maintain median time Neutralizing Neutralizing antibodies neutralizing antibody Neutralizing antibody titer neutralizing antibody titers Novel coronavirus novel coronavirus disease optimal dose oxyhemoglobin oxyhemoglobin saturation parameter Patient pilot project primary endpoint Radiological examinations randomized trial Seven severe adverse effect severe COVID-19 severe COVID-19 patient Severe patient severe patients Significance significantly supportive care the patient therapy transfused transfusion Treatment Treatment outcome trials undetectable Viral Viral load Viral RNA Viremia [DOI] 10.1073/pnas.2004168117 PMC 바로가기 [Article Type] 530
Identification of a novel B-cell epitope in the spike protein of porcine epidemic diarrhea virusResearch Published on 2020-04-032022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] 3D structure anorexia antibodies antibody Antisera B-cell epitope BALB/c mice cause characterized demonstrated detect determine Diagnostic method ELISA ENhance epidemic diarrhea epitope Epitopes generate high mortality identification identify IFA immunization immunize immunized mice immunodominant indicated Infection Infectious disease linear mAb monoclonal antibodies monoclonal antibody Neonatal Neutralizing Neutralizing antibodies PEDV peptide polyclonal polyclonal antibodies Protein purified reactivity receptor recognize Recombinant protein Region required residue Result S protein S1 protein Spike protein the spike protein Vaccine design virion virus vomiting was used weight loss were used western blot [DOI] 10.1186/s12985-020-01305-1 PMC 바로가기 [Article Type] Research
A new approach for therapeutic vaccination against chronic HBV infectionsArticle Published on 2020-03-302023-06-13 Journal: Vaccine [Category] B형 간염, [키워드] chronic HBV infection. CTL response HBcAg capsid Neutralizing antibodies oral vaccination Therapeutic vaccination TLM-peptide Transdermal vaccination vaccine platform virus-like particles [DOI] 10.1016/j.vaccine.2020.02.063