Analytical comparisons of SARS-COV-2 detection by qRT-PCR and ddPCR with multiple primer/probe setsLetter Published on 2020-06-042022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, MERS, SARS, [키워드] CDC center Chinese clinical sample comparison conditions Control Diagnosis digital PCR Dilution False negative False positive feasibility N gene Nucleic acid detection ORF1ab polymerase chain positive prevention qRT-PCR Quantitative real time real time PCR reduce Result SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 nucleic acid significantly specimens true negative Viral load [DOI] 10.1080/22221751.2020.1772679 PMC 바로가기 [Article Type] Letter
Evaluation of the commercially available LightMix® Modular E-gene kit using clinical and proficiency testing specimens for SARS-CoV-2 detectionArticle Published on 2020-05-272022-11-01 Journal: Journal of Clinical Virology [Category] COVID-19, MERS, SARS, [키워드] Algorithm analytical sensitivity assays Characteristics correlation CoV COVID-19 Culture cycle diagnostic Dilution E-gene evaluate evaluated Evaluation infection control LightMix E-gene limit of detection log management objective Patient PCR performance performed Rapid required Result RT-PCR assays SARS-CoV-2 SARS-CoV-2 detection sensitivity specimen TCID threshold threshold value Variation were used Wuhan [DOI] 10.1016/j.jcv.2020.104476 PMC 바로가기 [Article Type] Article
Treatment of 5 Critically Ill Patients With COVID-19 With Convalescent PlasmaResearch Published on 2020-04-282022-11-01 Journal: JAMA [Category] COVID-19, SARS, [키워드] acute respiratory distress acute respiratory syndrome Administered administration Admission age Antiviral antiviral agent Antiviral treatment approach ARDS assessment binding blood biochemical body temperature case sery change China Chinese patient clinical Clinical outcome clinical status clinical trials conducted convalescent plasma coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 Critical Critically ill patient determine Dilution discharged ECMO Effectiveness ELISA end point enzyme-linked immunosorbent exposure extracorporeal membrane oxygenation failure Final finding Follow-up greater hospital Human plasma IgG Ill Importance Infection Infectious disease laboratory-confirmed limited sample size measure mechanical ventilation Methylprednisolone Mortality neutralization titer neutralizing antibody Neutralizing antibody titer normalized objective observation outcome pandemic participant Patient patients plasma Plasma transfusion Point preliminary finding progression raise randomized clinical trials receiving recovered patients Relevance resolved Result SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2–specific antibody serum antibody setting severe pneumonia Shenzhen SOFA SOFA score Study design Support syndrome therapeutic agent treated Treatment ventilatory Viral load were given with COVID-19 women [DOI] 10.1001/jama.2020.4783 PMC 바로가기 [Article Type] Research
In vitro antiviral activity of fifteen plant extracts against avian infectious bronchitis virusResearch Article Published on 2019-05-292022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] antiviral activity Avian infectious bronchitis bronchitis Concentration cycle threshold cytopathogenic effect decrease Delta Dilution disease effective Efficacy Extract genotypes IBV Infection Infectious Bronchitis Virus Infectious disease limit log Mutation PCR plant plant extracts plants reduction Result RNA selected serotype significantly TCID tested Treatment virus virus plaque [DOI] 10.1186/s12917-019-1925-6 PMC 바로가기 [Article Type] Research Article
Evaluation of an accelerated hydrogen peroxide disinfectant to inactivate porcine epidemic diarrhea virus in swine feces on aluminum surfaces under freezing conditionsResearch Article Published on 2017-12-012022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] accelerated Accelerated hydrogen peroxide addition aluminum applied collected Concentration condition conditions Contact Contamination control group country determine Dilution Disinfection Efficacy epidemic diarrhea evaluated expected fecal glycol hydrogen peroxide inactivated infectious dose Infectivity inoculated negative and positive negative control nucleocapsid PEDV polymerase chain positive positive control Quantitative Randomly rectal swab representing Result reverse transcription RT-qPCR Seven Spread supplementary material Swab swine temperature Treatment treatment group virus was determined were used [DOI] 10.1186/s12917-017-1300-4 PMC 바로가기 [Article Type] Research Article
Environmental persistence of porcine coronaviruses in feed and feed ingredientsResearch Article Published on 2017-05-242022-10-28 Journal: PLoS ONE [Category] Coronavirus, MERS, [키워드] addition aliquot Blood Cell culture compared and analyzed Complete Concentration corn Corona coronavirus correlation Delta determine Dilution estimate ether Health inactivation incubated ingredient inoculated log Log reduction moderate negative correlation PEDV persistence plasma reduce Sample soybean survival temperature TGEV threat virus virus transmission Weibull model weighed [DOI] 10.1371/journal.pone.0178094 PMC 바로가기 [Article Type] Research Article
Experimental study of the relationship between Plasmodium gametocyte density and infection success in mosquitoes; implications for the evaluation of malaria transmission-reducing interventionsarticle Published on 2015-02-282024-09-01 Journal: Endocrine [Category] 말라리아, [키워드] Anopheles Dilution Gametocyte density infection success Plasmodium transmission blocking interventions [Article Type] article
Detection of swine transmissible gastroenteritis coronavirus using loop-mediated isothermal amplificationResearch Published on 2010-08-292022-10-28 Journal: Virology Journal [Category] Coronavirus, MERS, [키워드] comparable conserved coronavirus detect detection Detection limit digestion Dilution found gastroenteritis high accuracy isothermal amplification Loop-mediated isothermal amplification nucleic acid Nucleocapsid gene PCR primer RNA specificity Standard TGEV viruses [DOI] 10.1186/1743-422X-7-206 PMC 바로가기 [Article Type] Research
Avian coronavirus infectious bronchitis virus susceptibility to botanical oleoresins and essential oils in vitro and in vivoArticle Published on 2010-01-212022-11-01 Journal: Virus research [Category] Coronavirus, MERS, [키워드] addition Administering Botanical extracts Clinical sign Compound coronavirus CPE, cytopathic effects Dilution disease effective EID50, 50% embryo infectious dose ELISA, enzyme linked immunosorbent assay essential oils examined expected greater HMA, hexamethylene amiloride Host IBV IBV, infectious bronchitis virus in vitro in vivo Infection Infectious Bronchitis Virus investigated knowledge Laboratory lesion Massachusett MHV, mouse hepatitis virus Natural ingredients Oleoresins PBS, phosphate buffered saline protection reduced RFLP, restriction fragment length polymorphism RT-PCR, Reverse transcriptase-polymerase chain reaction SARS-CoV, Severe Acute Respiratory Syndrome virus serotype severity SPF, specific pathogen free susceptibility TCID50, 50% tissue culture infectious dose Transmission Treatment Vero E6 cell Viral RNA virucidal virus Virus challenge virus inactivation virus titer viruses was determined [DOI] 10.1016/j.virusres.2010.01.006 PMC 바로가기 [Article Type] Article