Cross-neutralization of RBD mutant strains of SARS-CoV-2 by convalescent patient derived antibodies회복기 환자 유래 항체에 의한 SARS-CoV-2의 RBD 돌연변이 균주의 교차 중화Article Published on 2021-11-012022-09-11 Journal: Biotechnology Journal [Category] MERS, SARS, 변종, 진단, [키워드] 293T cells ACE2 angiotensin angiotensin converting enzyme antibody Bin2 binding binding affinity Cell clone clones convalescent patient COVID-19 pandemic COVID-19 recovered patient enzyme epitope global pandemic group hACE2 high affinity IgG inhibitory Interaction live SARS-CoV-2 MOST mutant mutant strain mutants mutations N501Y neutralized Neutralizing Neutralizing antibodies neutralizing antibody overexpressed patient-derived antibody libraries. positive preventing infection pseudovirus entry RBD RBDs receptor Receptor binding domain SARS-CoV-2 SARS-CoV-2 strain screened spike Spike protein the antibody therapeutic interventions V367F Vero-E6 cells Viral viral infection wildtype [DOI] 10.1002/biot.202100207 PMC 바로가기 [Article Type] Article
A surrogate cell-based SARS-CoV-2 spike blocking assayArticle Published on 2021-11-012023-06-19 Journal: European Journal of Immunology [Category] MERS, SARS, [키워드] COVID-19 hACE2 SARS-CoV-2 Spike protein Surrogate blocking assay [DOI] 10.1002/eji.202149302 PMC 바로가기
Cyclic 68 Ga-Labeled Peptides for Specific Detection of Human Angiotensin-Converting Enzyme 2Basic Science Investigation Published on 2021-11-012022-10-30 Journal: Journal of Nuclear Medicine [Category] COVID-19, [키워드] ACE2 acute respiratory syndrome affected angiotensin-converting enzyme 2 ARDS binding biodistribution calculated Cohort compounds Concentration coronavirus coronavirus disease COVID-19 COVID-19 patients cyclic detect detection generate glycol hACE2 half-maximal inhibitory concentration Human humanized hypothese identify identity inhibitor linear linker liver lung Lungs modulation murine murine model organ organ injury organ-specific peptide pet Pharmacologic reduction residues SARS-CoV-2 SARS-COV-2 infection small intestine Specific susceptibility transgenic transgenic model Visual was determined was used [DOI] 10.2967/jnumed.120.261768 PMC 바로가기 [Article Type] Basic Science Investigation
Temporal-Geographical Dispersion of SARS-CoV-2 Spike Glycoprotein Variant Lineages and Their Functional Prediction Using in Silico ApproachResearch Article Published on 2021-10-262022-10-29 Journal: mBio [Category] COVID-19, MERS, SARS, [키워드] in silico prediction Alpha Amino acid amino acid change amino acid variation antigenicity association B.1.1.7 B.1.351 B.1.617 binding affinity binding domain collected Complete conformational change COVID-19 D614G dispersion E484K evade Evolution of SARS-CoV-2 genomic GISAID glycoprotein greater hACE2 Host hypothetical immune immunoinformatics immunological in silico information initiative lineages Mutation mutations N439K N501Y P.1 pandemic Positive selection positive-sense single-stranded RNA predicted prediction Protein residue S protein S protein variant SARS-CoV-2 SARS-CoV-2 variant sequence spike Surveillance the Spike Transmission Vaccines variants variants of concern Variation virus VOCs [DOI] 10.1128/mBio.02687-21 PMC 바로가기 [Article Type] Research Article
Rapid Detection and Inhibition of SARS‐CoV‐2‐Spike Mutation‐Mediated MicrothrombosisResearch Article Published on 2021-10-232022-10-28 Journal: Advanced Science [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome adverse outcome age antibodies binding Blood Blood coagulation boost can be used coated with coronavirus coronavirus disease COVID‐19 patients D614G detection Endothelial cell enzyme examined genetic heterogeneity hACE2 Host immune response in viral increasingly Infection Inflammation inhibition Microfluidic chip Microthrombosis outcomes Patient patients platform presence of comorbidity protective mechanism Protein Rapid reduce reported Safe SARS‐CoV‐2 SARS‐CoV‐2 infection SARS‐CoV‐2 spike SARS‐CoV‐2 sequence spike tested therapy thought thrombosis thrombotic complication thrombotic event Thrombotic events vaccination Vaccine variant vector Whole blood [DOI] 10.1002/advs.202103266 PMC 바로가기 [Article Type] Research Article
Evolutionary Perspective and Theories on the Possible Origin of SARS-CoV-2Infectious Disease Published on 2021-10-222022-10-31 Journal: Cureus [Category] COVID-19, [키워드] acute respiratory syndrome Algorithm Amino acid sequences analyses angiotensin-converting enzyme 2 coronavirus expectation furin-like cleavage site Genes hACE2 Host human Angiotensin-converting enzyme identify interact MERS-CoV meta-analyses Middle East Multiple sequence alignment Next-generation sequencing origin outbreak performed Phylogenetic analysis platform Protein RaTG13 RdRP respiratory syndrome coronavirus RNA-dependent RNA polymerase S1/S2 SARS-CoV SARS-CoV-2 sequence similarity subunit systematic review the SARS-CoV-2 the spike protein theory trajectory transfer vipr whole genome sequence zoonotic zoonotic transfer [DOI] 10.7759/cureus.18981 PMC 바로가기 [Article Type] Infectious Disease
COVA1-18 neutralizing antibody protects against SARS-CoV-2 in three preclinical models세 가지 전임상 모델에서 COVA1-18 중화 항체가 SARS-CoV-2로부터 보호Article Published on 2021-10-202022-09-10 Journal: Nature Communications [Category] MERS, SARS, 변종, 신약개발, 유전자 메커니즘, 진단, 치료제, [키워드] acute respiratory syndrome coronavirus Administered animal model antibody antiviral activity B.1.1.7 B.1.1.7 isolate cause clinical evaluation coronavirus COVID-19 patients cynomolgus macaques Efficacy evaluate hACE2 hamsters High dose Immunotherapy in vivo Infectious diseases lung lung lesions Lungs lymphopenia macaque mathematical mathematical model mice monoclonal antibodies neutralizing antibody patients Preclinical research Prophylactic PROTECT reduce reduction in respiratory SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 shown therapeutic therapeutic effect Therapeutic treatment treated Treatment undetectable upper respiratory upper respiratory tract Viral viral infectivity Viral load viral loads [DOI] 10.1038/s41467-021-26354-0 PMC 바로가기 [Article Type] Article
Molecular insights into receptor binding of recent emerging SARS-CoV-2 variants최근에 등장한 SARS-CoV-2 변이체의 수용체 결합에 대한 분자적 통찰력Article Published on 2021-10-202022-09-10 Journal: Nature Communications [Category] MERS, SARS, 변종, 치료제, [키워드] ACE2 ACE2-RBD Alpha angiotensin Beta binding binding affinity cells changes in complexes crystal structure crystal structures domain enzyme examined FIVE Gamma hACE2 hACE2 protein help human ACE2 human Angiotensin-converting enzyme identify increase in information Interaction lack molecular molecular basis Multiple mutants Mutation Prevent Prophylactic Protein pseudovirus pseudoviruses RBD receptor Receptor binding Receptor-binding domain residue S protein SARS-CoV-2 SARS-CoV-2 RBD SARS-CoV-2 spike SARS-CoV-2 variant susceptible the S protein therapeutic variant Viral viral entry Viral proteins virus transmissibility VoC VOCs X-ray crystallography [DOI] 10.1038/s41467-021-26401-w PMC 바로가기 [Article Type] Article
A potent bispecific nanobody protects hACE2 mice against SARS-CoV-2 infection via intranasal administration강력한 이중특이성 나노바디는 비강 투여를 통해 SARS-CoV-2 감염으로부터 hACE2 마우스를 보호합니다Article Published on 2021-10-192022-09-11 Journal: Cell Reports [Category] MERS, SARS, 변종, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition administration albumin alpaca bispecific circulating SARS-CoV-2 variants coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 Delta delta variant effective Efficacy exhibit hACE2 hACE2 mice Human serum albumin immunized IMPROVE in vivo intranasal Live virus mice nanobody Nb15 Nb15-NbH-Nb15 neutralization potency Neutralizing potent neutralizing. Prophylactic PROTECT provide Pseudotyped viruses RBD of SARS-CoV-2 Receptor binding domain respiratory SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variants severe acute respiratory syndrome Coronavirus spike glycoprotein therapeutic purpose therapeutic strategy transgenic treating COVID-19 Treatment trimer VHH wild-type [DOI] 10.1016/j.celrep.2021.109869 PMC 바로가기 [Article Type] Article
Computational prediction of the effect of amino acid changes on the binding affinity between SARS-CoV-2 spike RBD and human ACE2408 Published on 2021-10-192022-10-29 Journal: Proceedings of the National Academy of Sciences of [Category] COVID-19, SARS, [키워드] Accuracy ACE2 protein acute respiratory syndrome amino acid change association binding binding affinities binding affinity binding energy calculated can be used caused cellular entry change changes in circulating coronavirus correlation coefficient decrease energy term hACE2 help host adaptation hosts human ACE2 human Ace2 protein human Angiotensin-converting enzyme immune evasion implicated implication independent Infection Metrics MM-GBSA molecular Neural network offer predict predicted proceed public health RBD Receptor binding domain required SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 Spike RBD SARS-CoV-2 variant Significance Spike protein Spread the binding affinity the RBD the spike protein trajectory transmissible variant Van der Waals variant variants viral surface viral variant worsening [DOI] 10.1073/pnas.2106480118 PMC 바로가기 [Article Type] 408