The Strengths of Scanning Electron Microscopy in Deciphering SARS-CoV-2 Infectious CycleMicrobiology Published on 2020-08-192022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] Cell cell lysis cells classical comparable Complete complex contributed coronavirus cycle Diagnosis electron electron microscopy electronic microscopy expelled helping infectious cycle membrane fusion Microscopy New Particle Patient plasma membrane Post-infection powerful tool public health Replication SARS-CoV SARS-CoV-2 SARS-CoV-2 particle scanning scanning electron microscopy SEM significantly strength TEM Transmission Vero E6 cell VERO E6 cells viral infections virus [DOI] 10.3389/fmicb.2020.02014 PMC 바로가기 [Article Type] Microbiology
Construction of SARS-CoV-2 Virus-Like Particles by Mammalian Expression SystemBioengineering and Biotechnology Published on 2020-07-302022-10-31 Journal: Frontiers in Bioengineering and Biotechnology [Category] COVID-19, [키워드] construction COVID-19 disease expression Genetic glycosylation patterns HEK-293T cells lack mammalian membrane protein molecular Morphological morphological feature non-infectious Pandemics Particle Particles pathogenic viruses powerful tool Protein Release Research Safe SARS-CoV-2 SARS-CoV-2 VLP SARS-CoV-2 VLPs self-assemble shown small envelope structural proteins System vaccine candidate Vero E6 cell virion Virological virus virus-like particles VLP [DOI] 10.3389/fbioe.2020.00862 PMC 바로가기 [Article Type] Bioengineering and Biotechnology
Enacting national social distancing policies corresponds with dramatic reduction in COVID19 infection ratesResearch Article Published on 2020-07-302022-10-28 Journal: PLoS ONE [Category] COVID-19, MERS, SARS, [키워드] 95% CI adopted approaches average COVID19 disease spread Efficacy global healthcare implementation infection rate lack mathematical mitigate National observation outbreak powerful tool Prevent prophylactic measure reduced reduction reduction in resulting significantly social distancing Spread suggested the SARS-CoV-2 Transmission virus while [DOI] 10.1371/journal.pone.0236619 PMC 바로가기 [Article Type] Research Article
Autophagy and SARS-CoV-2 infection: A possible smart targeting of the autophagy pathwayEditorial Published on 2020-06-222022-10-28 Journal: Virulence [Category] COVID-19, MERS, SARS, [키워드] acting activate acute respiratory syndrome administration adverse effect Apoptosis apoptotic death apoptotic pathway approved autophagy autophagy flux Betacoronavirus Cell clinical trials confirmed case coronavirus cross-talk death disrupt drug drug targeting drugs Effect FDA-approved drug infected cell Infection inhibit Macroautophagy molecular mechanism nanomedicine nanoparticle Nanoparticles outbreak overcome pathway powerful tool SARS-CoV-2 SARS-COV-2 infection severe adverse effect Spread suggested Treatment virus virus infection virus replication viruses WHO [DOI] 10.1080/21505594.2020.1780088 PMC 바로가기 [Article Type] Editorial
A single-center, retrospective study of COVID-19 features in children: a descriptive investigationResearch Article Published on 2020-05-062022-10-28 Journal: BMC Medicine [Category] COVID-19, MERS, SARS, [키워드] absorbed addition Adults analyzed C-reactive protein Characteristics Chest CT children China clinical Clinical features clinical recovery collected Computerized tomography coronavirus COVID-19 COVID-19 infection Cox regression criteria CT findings detect discharge disease elevated Epidemiology Evidence feature Fisher exact test FIVE ground-glass opacity had no hemoglobin hospital discharge include initial Laboratory laboratory data lesion lesions less Local lower lobe lymphocytosis lymphopenia Negative PCR outbreak patchy Patient PCR pediatric Pediatric patient Pneumonia polymerase chain positive COVID-19 positive result powerful tool radiologic reported Result Retrospective study SARS-CoV-2 Seven single-center standard reference statistical subpleural subsequent unique utility Wuhan [DOI] 10.1186/s12916-020-01596-9 PMC 바로가기 [Article Type] Research Article
Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay역전사 루프 매개 등온 증폭 분석에 의한 2019 신종 코로나바이러스(SARS-CoV-2)의 신속하고 시각적인 검출Comparative Study Published on 2020-04-082022-09-25 Journal: Clinical microbiology and infection : the official [Category] SARS, 진단, [키워드] 2019 novel coronavirus 95% CI acute respiratory syndrome amplified Clinical diagnosis clinical samples clinically suspected close contact collected coronavirus COVID-19 cross-reactivity detection evaluate evaluated groups isothermal amplification MINI objective observation Patient positive powerful tool primer primer set Rapid Respiratory pathogens Result reverse transcription RNA RT-LAMP RT-PCR S gene SARS-CoV-2 SARS-CoV-2 detection SARS-COV-2 infection sensitivity Sensitivity and specificity specificity specimen suspected patients temperature the mean Total Viral RNA Visual [DOI] 10.1016/j.cmi.2020.04.001 PMC 바로가기 [Article Type] Comparative Study
Peer-to-Peer Contact Tracing: Development of a Privacy-Preserving Smartphone AppOriginal Paper Published on 2020-04-072022-10-31 Journal: JMIR mHealth and uHealth [Category] Coronavirus, SARS, [키워드] anonymously approach Consequences Contact tracing coronavirus Course COVID-19 COVID-19 pandemic death rates develop development disease spread disease transmission drastic effective Epidemic Epidemics information interactions interpersonal interaction Intervention Isolation limit measure mobile phone novel coronavirus disease objective outbreak pandemic Pandemics peer-to-peer personal data positive powerful tool privacy public health crisis Result risk Smartphone suppress trajectory virus [DOI] 10.2196/18936 PMC 바로가기 [Article Type] Original Paper
Attenuating the emergence of anti-fungal drug resistance by harnessing synthetic lethal interactions in a model organism모델 유기체에서 합성 치명적인 상호 작용을 활용하여 항진균제 내성의 출현을 완화Research Article Published on 2019-08-192022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] allele alleles antifungal drug resistance antifungal therapy antimicrobial resistance approach Bacteria best Candida Care caused Cell clinically combination therapies Community community acquired death Deletion drug drug resistance drug-resistant drugs effective Evolution experiments Express expressing FIVE fluconazole fungal Fungal infection fungal infections fungi Gain gain-of-function Genetic genetic component genetic screens genus growth hospitals Host identify Immunosuppressed inactivation incidence Infection infections inhibit inhibited Interaction investigated isolate knowledge life life-threatening mediator mediators Medical care Mortality MRSA mucosal mutant Mutation organism pathogen Pathogens patient population performed phenotype powerful tool Prevent proof proof of concept Protein provide resistance mechanisms responsible Saccharomyces cerevisiae significantly target target proteins Testing therapy thrush Treatment yeast [DOI] 10.1371/journal.pgen.1008259 PMC 바로가기 [Article Type] Research Article
Combined use of ELISA and Western blot with recombinant N protein is a powerful tool for the immunodiagnosis of avian infectious bronchitisResearch Published on 2018-12-122022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] amplified analysed antibody Antigen association bronchitis cDNA Cell Chromatography coding region coding sequence Combined combined use competent Concentration confidence interval controls demonstrated detect develop Diagnosis E. coli early diagnosis ELISA Escherichia coli evaluate examined expected expressed expression vector GE Healthcare GraphPad Prism GraphPad software greater IBV indirect ELISA Infectious Bronchitis Virus Invitrogen liquid molecular N protein nucleocapsid protein nucleoprotein PCR positive powerful tool Prevent primer Protein purification purified receiver-operating characteristics curves recombinant antigen required Result RT-PCR scored SDS-PAGE sensitivity sera serum dilution serum sample specificity subunit Test tested the disease the N protein USA was obtained were used Western blot analysis window yielding [DOI] 10.1186/s12985-018-1096-2 PMC 바로가기 [Article Type] Research
Genome-wide CRISPR-dCas9 screens in E . coli identify essential genes and phage host factorsE의 게놈 전체 CRISPR-dCas9 스크린. 대장균은 필수 유전자와 파지 숙주 인자를 식별합니다.Research Article Published on 2018-11-072022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] addition applied Bacteria Bacterial bacterial capsule bacterial gene bacterial genes bacteriophage bacteriophages capsids Cas9 Cas9 RNA-guided nuclease catalytic Cell chromosome colanic acid convenience CRISPR CRISPR-Ca CRISPR-dCas9 screen CRISPRi dCas9 dCas9 protein detect E . coli expression level Factor functional Gene Expression Genes Genetic genetic interactions genetic screens Genome genomes growth guide RNA High-throughput genetic screen High-throughput genetic screens highlight highlighting Host identify infection process Interaction involved kill library mechanism medium modulate mutant over pathway phage phage λ phages phages λ phenotype Phenotypes Plasmid powerful tool predict progeny random remained reported required resistance mechanism Screen sgRNA sgRNAs technique tested transduction assay utility [DOI] 10.1371/journal.pgen.1007749 PMC 바로가기 [Article Type] Research Article