Real-Time Sequence-Validated Loop-Mediated Isothermal Amplification Assays for Detection of Middle East Respiratory Syndrome Coronavirus (MERS-CoV)Research Article Published on 2015-04-092022-10-28 Journal: PLoS ONE [Category] Coronavirus, MERS, [키워드] accelerated acute respiratory illness amplicons amplification Amplification assay amplify applied assays asymmetric cause coronavirus cross-react detect detection diagnostics E gene facilitate Frame genomic infected cell culture Infection infections isothermal amplification loci management MERS-CoV Middle East mortality rate ORF1b gene plaque forming unit point-of-care primer probe reported respiratory Respiratory pathogens respiratory syndrome coronavirus resulting reverse transcription RT-LAMP supernatant upstream [DOI] 10.1371/journal.pone.0123126 PMC 바로가기 [Article Type] Research Article
Epigenome-Guided Analysis of the Transcriptome of Plaque Macrophages during Atherosclerosis Regression Reveals Activation of the Wnt Signaling Pathway죽상경화증 퇴행 동안 플라크 대식세포의 전사체에 대한 후성유전체 분석은 Wnt 신호 전달 경로의 활성화를 보여줍니다Research Article Published on 2014-12-042022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] activated Affect Analysis annotations approach arterial plaque macrophage arteries Atherosclerosis binding site canonical Cell cell motility cell type chromatin circulating condition control group demonstrated detect differentially expressed drug treatments eight element enrichment epigenomic eQTL Evidence expression foam cell functional Gene Expression humans identify in vitro in vivo increase Inflammation inflammatory disease inflammatory state innate immune system less Lipoproteins lowering Macrophage Macrophage response macrophage responses macrophages mice Migration molecular mouse model network overlap plaque plaque macrophage plaque macrophage response plaque macrophage responses plaque macrophages plaque regression plaques plasma lipoprotein plasma lipoproteins power promoter promoters reducing regressing plaque regressing plaques Regression Regression model regression models regulatory region response reversed risk significantly SNPs statins statistically Stimulation Systems biology TCF/LEF therapeutic target transcription factor transcription factors transcriptional change Transcriptome upregulated upregulated gene upregulated genes upstream validation study while Wnt Wnt signaling Wnt signaling pathway β-catenin [DOI] 10.1371/journal.pgen.1004828 PMC 바로가기 [Article Type] Research Article
Coronavirus Cell Entry Occurs through the Endo-/Lysosomal Pathway in a Proteolysis-Dependent MannerResearch Article Published on 2014-11-062022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] Cell cell entry Clathrin-mediated endocytosis cleavage conformational change coronavirus CoV CoVs demonstrated endocytosed endosomal maturation endosomal pH entry pathway expressing furin furin cleavage site fusion fusion peptide fusion protein gene silencing Genome Host host cell host cell membrane induce Infection influenza A virus inhibited introduced investigated late endosomes less lysosomal protease lysosomal proteases lysosome membrane fusion MERS MERS-CoV MHV microenvironment mouse hepatitis murine hepatitis virus negatively affected occur pathogen plasma membrane predicted previous study promote protease Protease inhibitor Protein proteolytic cleavage Proteolytic processing reporter required S protein shown siRNA Specific the S protein upstream vesicular stomatitis virus virus while zoonotic [DOI] 10.1371/journal.ppat.1004502 PMC 바로가기 [Article Type] Research Article
PRO40 Is a Scaffold Protein of the Cell Wall Integrity Pathway, Linking the MAP Kinase Module to the Upstream Activator Protein Kinase CPRO40은 MAP 키나제 모듈을 업스트림 활성제 단백질 키나제 C에 연결하는 세포벽 무결성 경로의 스캐폴드 단백질입니다.Research Article Published on 2014-09-042022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] activator adapter adapters Affinity purification Alzheimer's disease approach ascomycete asexual development bind Cancer Cell cell differentiation Cell fusion cell wall characterized combating conserved Control deletion mutant deletion mutants determinant developmental developmental processe developmental processes disease domain ENCODE environmental cue environmental cues eukaryote eukaryotes eukaryotic Evidence experiment Extensive filamentous fungi fruiting bodies fungal fungi fungus Genetic growth homolog implicated integrity Interaction interaction partner involved kinase MAPK mass spectrometry mitogen mitogen-activated protein kinase module molecular MOST mutant mutated N-terminal Pathogenesis pathogenicity pathway Pathways Phosphorylation polar Protein protein kinase protein kinase C Regulation required scaffold protein Signal transduction Signaling Sordaria macrospora Stress stress response symbiosis the cell upstream vegetative growth wall yeast [DOI] 10.1371/journal.pgen.1004582 PMC 바로가기 [Article Type] Research Article
Performance and clinical validation of the RealStar ® MERS-CoV Kit for detection of Middle East respiratory syndrome coronavirus RNA중동 호흡기 증후군 코로나바이러스 RNA 검출을 위한 RealStar ® MERS-CoV 키트의 성능 및 임상 검증Article Published on 2014-03-282022-09-25 Journal: Journal of Clinical Virology [Category] MERS, 진단, [키워드] Altona Diagnostics amplification analytical sensitivity assays clinical sample comparable coronavirus cross-reactivity Diagnosis diagnostic assay Diagnostic method envelope Epidemiology Frame genomic region Germany Hamburg highly pathogenic IMPROVE Internal control management MERS-CoV MERS-CoV infection MERS-CoV RNA Middle East Middle East respiratory syndrome molecular Munich nucleic acid objective ORF Patient PCR performance real-time RT-PCR Respiratory disease respiratory diseases respiratory pathogen respiratory sample respiratory syndrome coronavirus Result RNA RT-PCR assay RT-PCR kit target region upstream virus WHO World Health Organization [DOI] 10.1016/j.jcv.2014.03.012 PMC 바로가기 [Article Type] Article
Mapping of PARK2 and PACRG Overlapping Regulatory Region Reveals LD Structure and Functional Variants in Association with Leprosy in Unrelated Indian Population GroupsPARK2 및 PACRG 겹치는 규제 영역의 매핑은 관련 없는 인도 인구 그룹의 나병과 관련된 LD 구조 및 기능적 변이를 나타냅니다.Research Article Published on 2013-07-042022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, 한센병, [키워드] a major Affect assay association Author caused China clinical forms compared declined different disease disease pathogenesis Frequency Gene Gene Expression genetic background Genome genomic region granulomatous infection groups health problem heterogeneity Host human gene human genes impairment India Infection Infectious disease leprosy mapping Mycobacterium Mycobacterium leprae North organism other variant other variants PACRG Pathogenesis peripheral nerves Population population group population groups populations Prevalence provide public health reason for Region regulatory region replicated Replication reported reporter saturated several gene several genes significantly skin SNP SNPs Structure structures susceptibility the disease therapy upstream [DOI] 10.1371/journal.pgen.1003578 PMC 바로가기 [Article Type] Research Article
High-Resolution Transcriptome Maps Reveal Strain-Specific Regulatory Features of Multiple Campylobacter jejuni Isolates고해상도 전사체 지도는 여러 Campylobacter jejuni 분리주의 균주 특이적 조절 기능을 보여줍니다Research Article Published on 2013-05-162022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] adaptation addition Analysis analyzed approach Author automated Bacterial bioinformatics Campylobacter Campylobacter jejuni compared consequence conservation conserved contribute CRISPR different DNA ecological niche ecological niches eukaryote eukaryotes facilitate feature functional gastroenteritis Gene Gene Expression Gene regulation Genetic Genome genome sequence Genome sequences genomic variation genomic variations genotypic genotypic variations growth Host hosts humans immune system IMPROVE include integration isolate isolates lead Map maturation modulate Multiple organism Organisms Pathogens pathway phenotypic phenotypic diversity phenotypic variation promoter promoter regions promoters provide Regulation related reveal RNA RNA sequencing RNase similarities similarity small RNA SNP sRNA strain Strains stress conditions time-consuming transcribed transcriptional Transcriptome transcriptomes TSS unique upstream Variation whole-genome [DOI] 10.1371/journal.pgen.1003495 PMC 바로가기 [Article Type] Research Article
Regulation of Programmed Ribosomal Frameshifting by Co-Translational Refolding RNA HairpinsResearch Article Published on 2013-04-292022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] 293T cells base pair base-pair determined disrupt downstream Efficiency example functional Hairpin in vitro mechanism provide Regulation regulatory element required reveal RNA RNA structure SARS Coronavirus SARS-CoV sequence variation shown stability stimulated translational upstream yeast [DOI] 10.1371/journal.pone.0062283 PMC 바로가기 [Article Type] Research Article
MK2-Dependent p38b Signalling Protects Drosophila Hindgut Enterocytes against JNK-Induced Apoptosis under Chronic StressMK2-의존성 p38b 신호는 만성 스트레스 하에서 JNK 유도 세포자멸사로부터 Drosophila Hindgut Enterocytes를 보호합니다Research Article Published on 2011-08-042022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] absence addressed affected antimicrobial peptides Apoptosis atrophy Author branch cascade Cell chronic complex component contribute damage damaged descendants Deterioration Diseases Drosophila Elimination Enterocyte enterocytes epithelial epithelium event form genetic analysis Gut Hindgut homeostasis Impaired induce integrity intestinal Intestinal diseases intestinal epithelium intestinal stem cell Intestinal stem cells JNK kinases leads less manifested pathogen Pathogens pathway peptides peptides) Prevent proliferation PROTECT Protective Protein protein kinase replaced REPLACED BY Replenishment required salt Signal transduction stem cell stem cells Stress Stressor stressors subsequent suppresse Trigger triggers upregulated upstream [DOI] 10.1371/journal.pgen.1002168 PMC 바로가기 [Article Type] Research Article
PLP2 of Mouse Hepatitis Virus A59 (MHV-A59) Targets TBK1 to Negatively Regulate Cellular Type I Interferon Signaling PathwayResearch Article Published on 2011-02-182022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] Activation acute respiratory syndrome assays catalytic domain cells cellular complex conserved coronavirus cytoplasm deubiquitinase domain dose-dependent manner DUB ENCODE Evidence experiment finding identification IFN IFN signaling IFN signaling pathway IFN transcription IFN-β in vitro inactivated inactive Infection inhibit inhibited innate immune system IRF3 MHV-A59 mouse mouse hepatitis virus nonstructural protein 3 Nsp3 nuclear translocation overexpression Papain-like protease phosphorylate PLP2 Prevent principal Proteins receptor reduced Regulatory Replication reporter shown Spread supported target TBK1 TNF TRAF3 Treatment Type type I interferon upstream [DOI] 10.1371/journal.pone.0017192 PMC 바로가기 [Article Type] Research Article