A bioluminescent and homogeneous SARS-CoV-2 spike RBD and hACE2 interaction assay for antiviral screening and monitoring patient neutralizing antibody levels항 바이러스 스크리닝 및 모니터링 환자 중화 항체 수준에 대한 생물 발광 및 균질 한 SARS-COV-2 스파이크 RBD 및 HACE2 상호 작용 분석Article Published on 2021-09-162022-09-01 Journal: Scientific Reports [Category] SARS, 변종, 신약개발, 진단, 치료기술, 치료제, [키워드] ACE2 ACE2 binding Anti-RBD antibodies antibody Antiviral apparent affinity binding affinity Biological techniques bioluminescent Brazil can be used changes in Clinical research COVID-19 demonstrated doses Drug discovery E484K E484K mutation epitope escaped hACE2 homogeneous human ACE2 Immunity immunoassay immunology Infectious diseases inhibitor Interaction methodology miniprotein N501Y mutation NAb NAbs Nanoluc luciferase Neutralizing activity Neutralizing antibodies neutralizing antibody Patient persistence populations positive sample RBD RBD mutation RBD mutations Region Research SARS-CoV-2 SARS-CoV-2 Spike RBD secondary antibodies secondary antibody sera soluble ACE2 spike symptom onset tested the binding affinity USA utility vaccinated individual vaccinated individuals [DOI] 10.1038/s41598-021-97330-3 PMC 바로가기 [Article Type] Article
Live imaging of SARS-CoV-2 infection in mice reveals that neutralizing antibodies require Fc function for optimal efficacy생쥐에서 SARS-CoV-2 감염의 실시간 영상은 중화 항체가 최적의 효능을 위해 Fc 기능을 필요로 함을 보여줍니다Article Published on 2021-09-142022-09-11 Journal: Immunity [Category] MERS, SARS, 유전자 메커니즘, 진단, [키워드] addition Administered antibody applied bioluminescence imaging BLI Brain convalescent COVID-19 death Effect effective Efficacy Fab Fc effector functions Fc function function hACE2 highlight human ACE2 human ACE2 transgenic mice immune protection immunopathology in vivo indicated Infection Inflammatory response Inflammatory responses Interaction intranasally K18-hACE2 mice limit lung Lungs mechanism mice MONITOR Monocytes NAb NAbs nanoluciferase nasal Nasal cavity natural killer natural killer cell Natural killer cells neutralization Neutralizing Neutralizing antibodies neutralizing antibody Neutrophils organ Pathogenesis prevented Prophylaxis required resolved reveal SARS-CoV-2 SARS-COV-2 infection subject systemically therapy transgenic treating COVID-19 Treatment virus spread [DOI] 10.1016/j.immuni.2021.08.015 PMC 바로가기 [Article Type] Article
The self-assembled nanoparticle-based trimeric RBD mRNA vaccine elicits robust and durable protective immunity against SARS-CoV-2 in mice자가 조립 나노 입자 기반 삼량체 RBD mRNA 백신은 마우스에서 SARS-CoV-2에 대한 강력하고 내구성 있는 보호 면역을 유도합니다Article Published on 2021-09-092022-09-10 Journal: Signal Transduction and Targeted Therapy [Category] Fulltext, SARS, 변종, [키워드] addition Alpha B.1.1.7 B.1.351 Beta cellular response COVID-19 domain effective vaccine elicit elicited ferritin fused hACE2 Humoral immunity immunization Infectious diseases intramuscularly live SARS-CoV-2 low-dose mice mRNA mRNA vaccine mRNA vaccines multivalent vaccine neutralizing antibody produced protective immunity Protein provide provided RBD regimen robust Safe SARS-CoV-2 SARS-CoV-2 mutations SARS-CoV-2 spike Spread the RBD the SARS-CoV-2 trimer trimeric trimeric RBD Vaccine Vaccines variant variants [DOI] 10.1038/s41392-021-00750-w PMC 바로가기 [Article Type] Article
Telaprevir is a potential drug for repurposing against SARS-CoV-2: computational and in vitro studiesResearch article Published on 2021-09-092022-10-05 Journal: Heliyon [Category] 신약개발, [키워드] acting activity acute respiratory syndrome Analysis Anti-HCV Antiviral approach approval approved drug binding free energy bypasse cathepsin L Cell culture coronavirus Direct docked drug Drug repurposing drugs Evidence hACE2 HCV hepatitis C virus human Angiotensin-converting enzyme IC50 in silico in vitro in vitro study indications Molecular dynamics simulation MPro Pandemics protease Protease inhibitor receptor SARS-CoV-2 SARS-CoV-2 replication shown supported target targets telaprevir therapeutic virus [DOI] 10.1016/j.heliyon.2021.e07962 [Article Type] Research article
Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2Research Article Published on 2021-09-082022-10-30 Journal: Microbiology Spectrum [Category] COVID-19, MERS, SARS, [키워드] ACE2 ACE2 glycoprotein ACE2 receptor ACE2-spike binding addition antiviral therapy approach binding biological activities Cell cell entry receptor cell membrane coronavirus Critical demonstrated disrupt Endoplasmic reticulum enzyme examined expression function glycosylation hACE2 Human human ACE2 illness implicated Infection Interaction Mutagenesis N-glycan N-glycosylation N-linked glycan not affected offer property Protein Pseudotyped viruses receptor recruitment reduced reducing required role S proteins SARS Coronavirus SARS-CoV SARS-CoV S protein SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 S SARS-CoV-2 S protein subsequent Support surface susceptibility syncytium formation transmembrane glycoprotein Treatment type I understanding viral entry viral spike virus entry [DOI] 10.1128/Spectrum.01199-21 PMC 바로가기 [Article Type] Research Article
Interactions of the Receptor Binding Domain of SARS-CoV-2 Variants with hACE2: Insights from Molecular Docking Analysis and Molecular Dynamic SimulationArticle Published on 2021-09-072022-10-28 Journal: Biology [Category] COVID-19, [키워드] acquisition acute respiratory syndrome Affect affected affecting African Alpha variant approaches Beta Beta variant binding change circulation coronavirus Coronavirus-2 COVID-19 COVID-19 pandemic Delta delta variant determine docking dynamic Effect Epsilon Eta examined FIVE Gamma Gamma variant Genetic changes genomic hACE2 host cells human Angiotensin-converting enzyme insight Interaction investigated Iota Lambda molecular Mutation mutations pandemic Point mutation Protein RBD RBD binding receptor Receptor-binding domain required Research resulting SARS-CoV-2 SARS-CoV-2 variant Seven significantly simple Simulation Simulation study South the receptor-binding domain therapeutic therapeutic effect United Kingdom United State variant of concern variants variants of concern variants of interest VoC VOCs VOI VOIs wild-type [DOI] 10.3390/biology10090880 PMC 바로가기 [Article Type] Article
SARS-CoV-2 crosses the blood–brain barrier accompanied with basement membrane disruption without tight junctions alterationSARS-CoV-2는 밀착 접합 변경 없이 기저막 파괴와 함께 혈액-뇌 장벽을 통과합니다.Article Published on 2021-09-062022-09-10 Journal: Signal Transduction and Targeted Therapy [Category] Fulltext, SARS, 치료기술, 치료제, [키워드] accompanied Activation administration astrocytes basement membrane blood-brain barrier Blood–brain barrier Brain brains collagen cross Endothelial cell endothelial cells Evan Evans blue Evidence Experimental models of disease expression hACE2 hamster Human in vitro in vivo experiments infect Inflammatory factor inflammatory factors Inflammatory response Inflammatory responses K18-hACE2 transgenic mice marker membrane MMP9 not altered occludin occurred pathway perivascular permeability reported SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 RNA tight junction tight junctions transcellular pathway ultrastructure upregulated Vascular Vasculitis vessel was increased ZO-1 [DOI] 10.1038/s41392-021-00719-9 PMC 바로가기 [Article Type] Article
Adenovirus transduction to express human ACE2 causes obesity-specific morbidity in mice, impeding studies on the effect of host nutritional status on SARS-CoV-2 pathogenesisArticle Published on 2021-09-032023-06-16 Journal: Virology [Category] MERS, [키워드] COVID-19 Diet-induced obesity hACE2 pathology Replication-defective adenovirus tissue [DOI] 10.1016/j.virol.2021.08.014 PMC 바로가기 [Article Type] Article
A mouse model of lethal respiratory dysfunction for SARS-CoV-2 infectionSARS-CoV-2 감염에 대한 치명적인 호흡기 기능 장애의 마우스 모델Article Published on 2021-09-012022-09-11 Journal: Antiviral Research [Category] SARS, 변종, 비임상, 진단, [키워드] angiotensin animal model Brain brains Central nervous system CNS COVID-19 COVID-19 cases COVID-19 patient disease phenotype dysfunction enzyme expressing feature full genome sequencing genetic differences Genetic variant genetic variants Genome sequencing hACE2 Infection inoculation Isolation K18-hACE2 K18-hACE2 mice lung Lungs mice Mild mild to moderate moderate morphology Mortality pandemic plaque Pneumonia pulmonary disease Pulmonary infection replicate rodent SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 isolate SARS-CoV-2 isolates SARS-CoV-2 variant SARS-CoV-2 variants shown Spread Therapeutics transgenic mice Vaccine wild-type SARS-CoV-2 with COVID-19 [DOI] 10.1016/j.antiviral.2021.105138 PMC 바로가기 [Article Type] Article
Computational screening of 645 antiviral peptides against the receptor-binding domain of the spike protein in SARS-CoV-2SARS-CoV-2에서 스파이크 단백질의 수용체 결합 도메인에 대한 645개 항바이러스 펩티드의 컴퓨터 스크리닝Article Published on 2021-09-012022-09-11 Journal: Computers in biology and medicine [Category] SARS, 치료제, [키워드] angiotensin angiotensin converting enzyme angiotensin converting enzyme 2 anti-RBD Antiviral Antiviral agents antiviral peptide binding Contact contact with Critical domain effective Efficacy enzyme exhibited feature GLU GLY guideline hACE2 hACE2 receptor higher affinity Human human Angiotensin-converting enzyme Human angiotensin-converting enzyme 2 hydrogen hydrogen bonding hydrophobic hydrophobic interactions in vitro in vivo inhibit inhibition inhibitor inhibitors Interaction internalization molecular dynamics Molecular dynamics simulation peptide peptides predicted Protein RBD Receptor-binding domain residue S protein SARS-CoV 2 SARS-CoV-2 SARS-CoV-2 S protein SARS-CoV-2 spike screened selected Small molecules Spike protein stable complex surface area the RBD the receptor-binding domain the S protein the spike protein Tolerability TYR virus [DOI] 10.1016/j.compbiomed.2021.104759 PMC 바로가기 [Article Type] Article