Immunoreactivity characterisation of the three structural regions of the human coronavirus OC43 nucleocapsid protein by Western blot: Implications for the diagnosis of coronavirus infectionArticle Published on 2012-11-192022-11-01 Journal: Journal of virological methods [Category] Coronavirus, MERS, [키워드] Adults antibody Antigen antigenicity Betacoronavirus Blood blood sample C-terminal domain characterisation Coronavirus infection cross-react demonstrated detected antibody Diagnosis diagnostic different E, Envelope Elderly patient epidemiological evaluated greater HCoV-229E HCoV-OC43 HCoV-OC43, human coronavirus OC43 human coronavirus human coronavirus OC43 human CoV human serum humans identify immune response immunoreactivity Infection IPTG, β-d-1-thiogalactopyranoside M, matrix N-terminal domain NP, nucleocapsid protein nucleocapsid protein OC43 strain occur offer patients polyclonal polyclonal antibody previous study produced Protein purified rabbit polyclonal antibody reactivity Region reported respiratory infection RNP, ribonucleoprotein S, spike SARS-CoV serum serum sample specificity SPR, surface plasmon resonance Strong tested Variability virus infection [DOI] 10.1016/j.jviromet.2012.11.009 PMC 바로가기 [Article Type] Article
Characterization of Human Coronavirus Etiology in Chinese Adults with Acute Upper Respiratory Tract Infection by Real-Time RT-PCR AssaysResearch Article Published on 2012-06-152022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] 229E addition Adult Beijing characterization characterized China Chinese clinical impact collected contribute Coronaviruses detected detection rate etiology finding Frequency HCoV-229E HKU1 human coronavirus human parainfluenza virus Infection Influenza led to less métapneumovirus molecular Nasopharyngeal swab NL63 OC43 Patient positive positive patient prevalent real-time RT-PCR related respiratory Respiratory pathogens respiratory virus rhinovirus RSV RT-PCR sensitivity significantly specimen suggested Surveillance tested tract Undifferentiated upper upper respiratory tract URTI Variation virus viruses [DOI] 10.1371/journal.pone.0038638 PMC 바로가기 [Article Type] Research Article
Co-circulation of Four Human Coronaviruses (HCoVs) in Queensland Children with Acute Respiratory Tract Illnesses in 2004Article Published on 2012-04-232022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] aetiology age ARI assay assigned children circulation clinical impact clinical severity score compared coronavirus detected develop diagnostic different HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 Human infectious Patient peaked positive random Real-time PCR reduce respiratory Respiratory illness respiratory specimen respiratory syncytial virus respiratory virus RT-rtPCR Screening serology Severity Score specimen tested tract transcriptase virus were used [DOI] 10.3390/v4040637 PMC 바로가기 [Article Type] Article
Reverse Genetics of SARS-Related Coronavirus Using Vaccinia Virus-Based RecombinationResearch Article Published on 2012-03-072022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] absence acute respiratory syndrome Analysis antiviral drug approach caused cDNA Cell characterization classical cloned Comparative analysis culture supernatant dendritic cell effective expressing facilitate Gene Expression generate Genome HCoV-229E Health Heterologous human coronavirus 229E in vitro transcription infectious Infectious virus led to less original form outbreak parental pathogenic plaque produced public health measures released Renilla luciferase Renilla luciferase gene Replication replication kinetics reporter reverse genetic system risk RNA transcript RNA transcripts robust SARS-CoV SARS-CoV Frankfurt-1 SARS-related coronavirus severe transfection Vaccines vaccinia vaccinia virus vectors virulent virus zoonotic disease [DOI] 10.1371/journal.pone.0032857 PMC 바로가기 [Article Type] Research Article
Etiology and Clinical Characterization of Respiratory Virus Infections in Adult Patients Attending an Emergency Department in BeijingResearch Article Published on 2012-02-282022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] addressed Adult assay Attending Beijing characterization checked China clinical clinical symptom conducted Department detected determine different dominant effective Emergency epidemiological finding HCoV-229E Health highlight human coronavirus Influenza virus métapneumovirus nose parainfluenza pathogen Patient picornavirus polymerase chain positive Prevalence profile real-time RT-PCR reported respiratory respiratory syncytial virus respiratory tract infection respiratory virus Respiratory virus infection respiratory viruses reverse transcription RSV Safe significantly tested Therapeutic approach throat swab virus virus infection viruses [DOI] 10.1371/journal.pone.0032174 PMC 바로가기 [Article Type] Research Article
The dominance of human coronavirus OC43 and NL63 infections in infantsArticle Published on 2011-12-192022-11-01 Journal: Journal of Clinical Virology [Category] Coronavirus, MERS, [키워드] 229E addition childhood children determine disease elicit Epidemiology explain Frequency HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 healthy children HKU1 hospital Hospitalized human coronavirus human coronavirus OC43 Immunity indication Infant infect infecting Infection infections less majority mild infection natural infection Newborn NL63 objective OC43 occur pathogenic positive PROTECT real time respiratory infection respiratory tract Result RT-PCR sequence Seroconversion seroconverted serology severe disease study group subsequent was determined [DOI] 10.1016/j.jcv.2011.11.011 PMC 바로가기 [Article Type] Article
Human coronavirus NL63: a clinically important virus?Special Report Published on 2011-02-022022-10-31 Journal: Future Microbiology [Category] Coronavirus, [키워드] cause childhood viral infections children Clinical features clinical symptom clinically coronavirus detected diagnoses disease FIVE HCoV-229E HCoV-NL63 HCoV-OC43 Human human coronavirus Immunocompromised incidence infect Infection infections knowledge Lower respiratory tract lower respiratory tract infection morbidity and mortality NL63 Occurrence Other respiratory viruses Prevalence respiratory respiratory tract infection respiratory viruses SARS-CoV shown upper respiratory tract upper respiratory tract infection [DOI] 10.2217/fmb.10.166 PMC 바로가기 [Article Type] Special Report
Human coronaviruses are uncommon in patients with gastrointestinal illnessArticle Published on 2010-04-012022-11-01 Journal: Journal of Clinical Virology [Category] Coronavirus, MERS, [키워드] Adult children clinical clinical manifestation Clostridium difficile collected coronavirus detected diarrhea disease emesis Frequency gastrointestinal disease HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 HKU1 hospital Human illness implicated included individual infect infectious median neurologic NL63 NO objective occurred Patient pediatric positive positive patient positive sample presenting primer set recorded respiratory complaints respiratory finding respiratory tract Result RNA extraction RT-PCR Sample screened Stool submitted variety Viral gastroenteritis was performed [DOI] 10.1016/j.jcv.2010.03.007 PMC 바로가기 [Article Type] Article
Effects of Coronavirus Infections in ChildrenPerspective Published on 2010-02-012022-10-31 Journal: Emerging Infectious Diseases [Category] Coronavirus, MERS, [키워드] acute respiratory syndrome caused Cell Central nervous system childhood circulating Community coronavirus CoV detect different early stage Effect emerging viruses Epidemiology facilitate HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 host range human coronaviruses human CoV infect Infection Isolation led to Manifestations Measures mutate pathogenicity Perspective Prevent recombine respiratory viruses RNA viruses SARS Spread strain Strains therapeutic These viruses virulent virus viruses [DOI] 10.3201/eid1602.090469 PMC 바로가기 [Article Type] Perspective
Coronaviruses in Children, GreeceLetters to the Editor Published on 2007-06-012022-10-31 Journal: Emerging Infectious Diseases [Category] Coronavirus, MERS, [키워드] children coronavirus Greece HCoV-229E HCoV-NL63 HCoV-OC43 letter respiratory infection [DOI] 10.3201/eid1306.061353 PMC 바로가기 [Article Type] Letters to the Editor