Hybrid physiologically-based pharmacokinetic model for remdesivir: Application to SARS-CoV-2렘데시비르에 대한 하이브리드 생리학적 기반 약동학 모델: SARS-CoV-2에 적용Article Published on 2021-05-012022-09-11 Journal: Clinical and translational science [Category] SARS, 치료제, [키워드] anti-SARS-CoV-2 Antiviral agents application can be used catastrophic caused Concentration condition conducted coronavirus disease Coronavirus disease 2019 COVID-19 determine effective Extracellular half-maximal effective concentration Health humans hybrid impacted imperative in vitro indicated lung Lungs metabolite mononuclear cell Multiple-dose Novel coronavirus pandemic parameter patients Peripheral blood Peripheral blood mononuclear cells pharmacokinetic prodrug profiles ranged RDV regimen regimens Remdesivir reported SARS-CoV-2 sensitivity analysis Severe acute respiratory syndrome target tissues Therapeutic treatment tissue [DOI] 10.1111/cts.12975 PMC 바로가기 [Article Type] Article
Mini-Factor H Modulates Complement-Dependent IL-6 and IL-10 Release in an Immune Cell Culture (PBMC) Model: Potential Benefits Against Cytokine StormImmunology Published on 2021-04-282022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, MERS, SARS, [키워드] Activation Against anaphylatoxins Anti-inflammatory autologous bacterial lipopolysaccharide C3a C5a CAR-T cell therapy Clinical use complement complement activation/inhibition complement inhibitors consequence COVID-19 cytokine release Cytokine release syndrome Cytokines differential inhibition EDTA Effect Factor H heat inactivation identify IL-10 IL-12 IL-13 IL-1α IL-1β IL-5 IL-6 IL-7 IL-8 immune immune stimulation impacts in vitro in vitro study inhibition innate immune innate immune system life-threatening LPS marker mononuclear cell PBMC PBMCs Peripheral blood Potential Proinflammatory cytokine Release SC5b-9 secretion serum storm subsequent therapy TNFα utility Viral diseases whole blood assay zymosan [DOI] 10.3389/fimmu.2021.642860 PMC 바로가기 [Article Type] Immunology
Analysis of the Long-Term Impact on Cellular Immunity in COVID-19-Recovered Individuals Reveals a Profound NKT Cell ImpairmentCOVID-19에서 회복된 개인의 세포 면역에 대한 장기적인 영향 분석은 심각한 NKT 세포 손상을 보여줍니다Research Article Published on 2021-04-272022-09-10 Journal: mBio [Category] MERS, SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 affected analyzed caused CD4 CD4 and CD8 T cells CD8 T cell CD8 T cells Cell cell death cells cellular cellular immunity characterized Concomitant convalescence coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 patient COVID-19-convalescent individual COVID-19-convalescent individuals cytokine Cytokines decrease defined disease expression frequencies Frequency granzyme B GzmB immune signature immune system immunological change immunological changes Impact increase in increases in individual Interaction Ki67 Ki67 expression mononuclear cell mononuclear cells NKT NKT cell pandemic Patient Peripheral blood Peripheral blood mononuclear cells proliferate reduction in Regulatory Regulatory T cell respiratory SARS-CoV-2 SARS-COV-2 infection severe acute respiratory syndrome Coronavirus Significant significant decrease significantly sustained T cell T cell receptor T cells TCR the disease the patient while Wuhan [DOI] 10.1128/mBio.00085-21 PMC 바로가기 [Article Type] Research Article
Designing a SARS-CoV-2 T-Cell-Inducing Vaccine for High-Risk Patient GroupsArticle Published on 2021-04-242022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] adjuvant Administered Blood CD4 clinical trial COVID-19 eight ELISpot assay group healthy high-risk patient High-risk patients HLA molecules HLA-DR intracellular cytokine staining lipopeptide lymphocyte mononuclear cell peptide peptide vaccine performed peripheral predicted predominant SARS-CoV-2 second set single subcutaneous injection Support T cell epitope T cell response TLR vaccination Vaccine weakened were assessed [DOI] 10.3390/vaccines9050428 PMC 바로가기 [Article Type] Article
In vitro cell culture model of human nasal-associated lymphoid tissue (NALT) to evaluate the humoral immune response to SARS-CoV-2 spike proteinsOriginal Article Published on 2021-04-242022-10-28 Journal: Saudi Journal of Biological Sciences [Category] COVID-19, [키워드] absence anti-S IgG antibody concentration Antibody Response Antigen appear candidate vaccine Cell Cell culture cells Cellular immune response co-cultured complete medium concerning coronavirus disease COVID-19 Culture demonstrated effort ELISA enzyme-linked immunosorbent assay evaluate full-length S full-length S protein Germany health authorities humoral immune response IgA IgM in vitro induce intranasally Lymphoid tissue Mild mononuclear cell mucosal natural infection nucleocapsid pandemic pathogenicity Patient Protein Proteins Result RPMI S protein S1 and S2 S1 subunit S2 subunit SARS-CoV-2 SARS-CoV-2 spike protein Saudi Arabia scientist secreted separated serious complication severe disease Significant subunit the S protein the SARS-CoV-2 tonsil used to evaluate Vaccine viral antigens Viral protein viral spike virus was used [DOI] 10.1016/j.sjbs.2021.04.051 PMC 바로가기 [Article Type] Original Article
Dysregulated Innate and Adaptive Immune Responses Discriminate Disease Severity in COVID-19조절되지 않는 선천 및 적응 면역 반응이 COVID-19의 질병 중증도를 구별합니다Article Published on 2021-04-232022-09-11 Journal: The Journal of Infectious Diseases [Category] SARS, 바이오마커, [키워드] abrogated adaptive Adaptive immunity Analysis Biomarkers Cell Clustering analysis COVID-19 COVID-19 disease COVID-19 disease severity COVID-19 patients Critical Critically ill critically ill patients cytokine cytokine production Cytokines disease disease severity exhaustion markers flow cytometry hepatocyte growth factor highlight highlighting host response Hyperinflammatory immune response immune responses Inflammatory Inflammatory responses Innate innate immune innate immune response innate immune responses Innate immunity interferon interferon-γ interleukin interleukin-17 lymphopenia mononuclear cell performed Peripheral blood Plasma cytokine Potential biomarker potential biomarkers proinflammatory proteomics provide response Result stem cell stem cell factor T-cell exhaustion Variation Whole blood [DOI] 10.1093/infdis/jiab065 PMC 바로가기 [Article Type] Article
Single-cell multi-omics analysis of the immune response in COVID-19COVID-19의 면역 반응에 대한 단일 세포 다중 오믹스 분석Article Published on 2021-04-202022-09-10 Journal: Nature Medicine [Category] MERS, SARS, 변종, 유전자 메커니즘, 진단, 치료기술, [키워드] accompanied acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 alteration alveolar alveolar macrophage Analysis Antigen antigen receptor B lymphocyte blood immune cell blood sample Blood samples C1QA cause CD16 CD34 CD8 Cell cellular component characterized circulating Cohort complement contribute coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 pathogenesis cross-sectional disease disease severity effector memory effector T cell effector T cells expansion expressing follicular Haematopoiesis helper T cell hematopoietic progenitor cell highlight IgA2 Immune cell immune cells immune response individuals with COVID-19 Infection lymphocyte Macrophage megakaryopoiesis memory T cells Mild Mild disease monocyte Monocytes mononuclear cell mononuclear cells Patient performed Peripheral blood Peripheral blood mononuclear cells Plasma cells plasmablast plasmablasts Platelet platelet activation Platelets predicted proteome proteomic provide receptor reveal RNA sequencing SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe disease severity single-cell symptomatic disease T cell T cells therapy transcript Transcriptome Viral viral infection viral infections [DOI] 10.1038/s41591-021-01329-2 PMC 바로가기 [Article Type] Article
Induction of Exaggerated Cytokine Production in Human Peripheral Blood Mononuclear Cells by a Recombinant SARS-CoV-2 Spike Glycoprotein S1 and Its Inhibition by DexamethasoneOriginal Article Published on 2021-04-162022-10-30 Journal: Inflammation [Category] COVID-19, MERS, SARS, [키워드] Activation anti-inflammatory activity blocked caused clinical benefit COVID-19 Cytokine storm Cytokines Degradation Dexamethasone DNA binding Effect experiment Human IL-1β IL-6 IL-8 increase in induction Inflammation Inflammatory inhibition investigated involved IκBα MAPK mechanism molecular mononuclear cell NF-KB NF-κB NF-κB activation NLRP3 inflammasome p65 PBMC PBMCs peripheral Peripheral blood pharmacological target Phosphorylation pro-inflammatory cytokine produced Production recombinant reducing reduction reduction in SARS-CoV-2 SARS-CoV-2 cytokine storm SARS-CoV-2 spike glycoprotein SARS-CoV-2 spike glycoprotein S1 SARS-CoV-2 virus infection significantly increased spike spike glycoprotein stimulated Stimulation TNFα was reduced [DOI] 10.1007/s10753-021-01464-5 PMC 바로가기 [Article Type] Original Article
Single-Cell RNA Sequencing Analysis of the Immunometabolic Rewiring and Immunopathogenesis of Coronavirus Disease 20192019년 코로나바이러스 질병의 면역대사 재배선 및 면역병인에 대한 단일 세포 RNA 시퀀싱 분석Immunology Published on 2021-04-142022-09-11 Journal: Frontiers in Immunology [Category] MERS, 바이오마커, 유전자 메커니즘, 진단, [키워드] antibody secretion ATP13A3 ATP6V1B2 biological processe CD14 Cell cell population cell survival change contribute coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 patient COVID-19 patients Degradation differentiation elevated exacerbated expressed feature Glycolysis hyperinflammation Immune cell immune cells immune dysfunction Inflammation inhibit investigated involved KMT2E LDHA lead lysine mechanism memory B cell memory B cells metabolic changes mild patients mono monocyte Monocytes mononuclear cell mononuclear cells NSD1 Pathways Patient PBMCs Peripheral blood Peripheral blood mononuclear cells PGD PKM Plasma cell Plasma cells plasmablast plasmablasts progression promoted reduced RNA sequencing data SETD2 severe COVID-19 severe disease Severe patient severe patients signaling pathways Single-cell RNA sequencing survival TKT Transcription [DOI] 10.3389/fimmu.2021.651656 PMC 바로가기 [Article Type] Immunology
Acute and post-acute phase of COVID-19: Analyzing expression patterns of miRNA-29a-3p, 146a-3p, 155-5p, and let-7b-3p in PBMCComparative Study Published on 2021-04-062022-10-04 Journal: International immunopharmacology [Category] MERS, 바이오마커, [키워드] acute COVID-19 acute respiratory syndrome addition Analysis appear assays Biomarker cellular miRNA characteristic coronavirus coronavirus disease COVID-19 COVID-19 diagnosis COVID-19 disease COVID-19 patient COVID-19 patients COVID‐19 demonstrated Diagnosis diagnosis and monitoring disease evaluated expression level expression pattern groups healthy information microRNA miRNA miRNA expression miRNA profile mononuclear cell pathogen PBMC PBMCs Peripheral blood Real-time PCR Result ROC ROC analysis SARS-CoV-2 selected sensitivity significantly significantly higher specificity suggested the disease the healthy was tested [DOI] 10.1016/j.intimp.2021.107641 PMC 바로가기 [Article Type] Comparative Study