Identification of the immunodominant neutralizing regions in the spike glycoprotein of porcine deltacoronavirusArticle Published on 2019-12-062022-11-01 Journal: Virus research [Category] Coronavirus, MERS, [키워드] Amino acid Antibody Response Antisera C-terminal domain coronavirus CTD defined E. coli effective effective therapy Epitope region expression fluorescent Host identification immunodominant in vitro in vivo Infection inhibit mice N-terminal domain neutralization Neutralization assay Neutralizing Neutralizing antibodies neutralizing antibody NTD Porcine deltacoronavirus porcine deltacoronavirus (PDCoV) produced Protein Recombinant protein Region S protein S1 subunit S2 subunit spike glycoprotein the S protein the S1 subunit Transmission Vaccine Vaccines Virus neutralization were expressed [DOI] 10.1016/j.virusres.2019.197834 PMC 바로가기 [Article Type] Article
Structural definition of a neutralization epitope on the N-terminal domain of MERS-CoV spike glycoproteinArticle Published on 2019-07-112022-10-29 Journal: Nature Communications [Category] Coronavirus, MERS, [키워드] Analysis antibody bind binding cell entry cellular receptor characterized conformational change Critical dependent on Dipeptidyl peptidase 4 DPP4 Effect epitope experiment explain functional help inhibit inhibiting mAb mechanism MERS-CoV Middle East monoclonal antibody MOST mutagenesis experiment N-terminal domain neutralization neutralization breadth neutralizing antibody NTD pre-fusion RBD RBD-targeting antibodies Region residue respiratory syndrome coronavirus spike glycoprotein Strains Structure synergistic the receptor-binding domain viral cell Virology X-ray crystallography [DOI] 10.1038/s41467-019-10897-4 PMC 바로가기 [Article Type] Article
Discovery of a rich gene pool of bat SARS-related coronaviruses provides new insights into the origin of SARS coronavirusResearch Article Published on 2017-11-302022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, MERS, [키워드] ACE2 addition Analysis bat block cave cell entry China circulating demonstrated Diseases domain Evidence Evolution exhibiting full-length genome full-length genome sequence Genetic Genome highlight Host Human human ACE2 Increasing N-terminal domain non-structural protein NTD offer ORF1a ORF3 ORF3b ORF8 ORF8a precursor provide RBD receptor recombination event reveal risk S gene S protein SARS Coronavirus SARS-CoV SARS-related coronavirus SARSr-CoV SARSr-CoVs sequence similarity strain Strains Support Surveillance thought Transmission unlikely [DOI] 10.1371/journal.ppat.1006698 PMC 바로가기 [Article Type] Research Article
A novel neutralizing monoclonal antibody targeting the N-terminal domain of the MERS-CoV spike proteinOriginal Article Published on 2017-05-242022-10-31 Journal: Emerging Microbes & Infections [Category] Coronavirus, MERS, [키워드] addition affected bind caused coronavirus epitope fatal infections humans Immunotherapy implication inhibitory concentration mAb mAbs mapped MERS-CoV MERS-CoV infection MERS-CoV S1 Middle East monoclonal monoclonal antibody N-terminal domain Neutralizing Neutralizing activity neutralizing epitope neutralizing monoclonal antibody NTD pandemic Prophylactic Protein public health RBD Region respiratory syndrome coronavirus Spike protein subunit the receptor-binding domain the S protein therapeutic Transmission Vaccine design viral infection virus wild-type zoonotic origin [DOI] 10.1038/emi.2017.18 PMC 바로가기 [Article Type] Original Article
Genomes of Fasciola hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria Related to the Agents of Potomac Horse and Human Sennetsu FeversPotomac Horse 및 Human Sennetsu Fever의 대리인과 관련된 Neorickettsia Endobacteria로 아메리카 대륙에서 Fasciola hepatica의 게놈이 식민지를 나타냅니다.Research Article Published on 2017-01-062022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] agent America Bacteria biliary tract Biology cattle cestodes colonization Comparative genomics Complete contig disease etiological agent Evolution facilitate Fasciola hepatica Fascioliasis female reproductive tract fevers fluke food food chain Gene Expression Genome genome sequence genomes genus Health hepatica heterogeneous horizontal transmission horse Host Human humans indicated Interaction interactions involved isolate less lining liver liver fluke mammalian manuscript mechanism Migration Neorickettsia NTD NTDs offered oral sucker oral suckers organ organs parasite pathogen Pathogens pathophysiology platyhelminth potential mechanism predicted Protein reference genome reported reproductive resource ruminant ruminants schistosome seminal vesicle sequence Sequencing sheep species surface protein survival tissue tissues Transmission transmitted trematode tropical Tropical Diseases variety vertical transmission Vesicle zoonotic [DOI] 10.1371/journal.pgen.1006537 PMC 바로가기 [Article Type] Research Article
Characterization of anti-MERS-CoV antibodies against various recombinant structural antigens of MERS-CoV in an imported case in ChinaOriginal Article Published on 2016-11-092022-10-31 Journal: Emerging Microbes & Infections [Category] Coronavirus, [키워드] antibody Antibody titer Antigen characterization China correlated correlation domain ELISA ELISAs enzyme-linked immunosorbent enzyme-linked immunosorbent assay Extracellular inactivated Infection MERS MERS coronavirus MERS-CoV Middle East N-terminal domain neutralization neutralizing antibody NTD occurred Patient pseudovirus RBD RBD ELISA recombinant structural antigens sera Seroconversion significantly Strong syndrome the patient [DOI] 10.1038/emi.2016.114 PMC 바로가기 [Article Type] Original Article
Identification and Comparison of Receptor Binding Characteristics of the Spike Protein of Two Porcine Epidemic Diarrhea Virus StrainsArticle Published on 2016-02-232022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] alphacoronavirus aminopeptidase N antiviral drug association C-terminal domain caused CHGD-01 strain coronavirus CoV CTD CV777 strain demonstrated domain epidemic diarrhea exhibited gastroenteritis HCoV-NL63 host cells identification Interaction investigated N-terminal domain NL63 NTD pAPN Pathogenesis PEDV Protein RBD receptor Receptor binding Receptor binding domain S protein S1 domain strain Strategy sugar sugars TGEV the S protein the Spike understanding Vaccine variant virus [DOI] 10.3390/v8030055 PMC 바로가기 [Article Type] Article
Genetic drift of human coronavirus OC43 spike gene during adaptive evolutionArticle Published on 2015-06-222022-10-29 Journal: Scientific Reports [Category] Coronavirus, [키워드] adaptive Analysis binding binding domain characterized coronavirus CoV CoVs Critical dN/dS dominant Epidemic Evolution FIVE Genetic genetic drift Genotype HCoV-OC43 Health human coronavirus OC43 human population human populations majority mechanism N-terminal domain NTD OC43 persistence phylodynamic Phylogenetic Positive selection prevalent S gene S protein Seven spike gene Substitution sugar the S1 subunit [DOI] 10.1038/srep11451 PMC 바로가기 [Article Type] Article
Genome-Wide Association Mapping in Dogs Enables Identification of the Homeobox Gene, NKX2-8 , as a Genetic Component of Neural Tube Defects in Humans개의 게놈 전반에 걸친 연관 매핑을 통해 인간의 신경관 결함의 유전적 구성 요소인 호메오박스 유전자 NKX2-8 식별 가능Research Article Published on 2013-07-182022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] addition affected Analysis animal animal models Author birth defects Brain canine Care caused Central nervous system chromosome closure codon Combination comparable complex component condition contribute costly defect develop disease dog during pregnancy dysraphism embryonic ENCODE environmental factors etiology Evidence ExoN exons expected explain expressed failure found frameshift frameshift mutation general term Genes Genetic Genetic studies genetics Genome genome-wide association genomic region home environment Homeobox Human human population human populations humans identification identify in addition to interactions lead malformation mapped mapping mechanism Medical care molecular basis morbidity and mortality multifactorial Mutation Neural neural tube Neural tube defect Neural tube defects NKX2 NKX2-8 NTD NTDs occur pathology Patient performed predicted Pregnancy premature stop codon Protein rare variant Rare variants receive Regulatory regulatory gene reported resulting secondary to sequenced Sequencing significantly skin Soft tissue spina bifida spinal cord spinal dysraphism Structure Taking tissue tissues tube vertebral column Weimaraner [DOI] 10.1371/journal.pgen.1003646 PMC 바로가기 [Article Type] Research Article