Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2 Research Published on 2020-07-132022-10-05 Journal: Nature Structural & Molecular Biology [Category] SARS, 유전자 메커니즘, 진단, [키워드] ACE2 ACE2 binding antibody bind binding domain cause Cell complex coronavirus CR3022 cryo-EM crystal structure epitope fusion H11-H4 human Angiotensin-converting enzyme Influenza Interaction maturation nanobodies nanobody neutralization Neutralizing activity overlap produced RBD receptor recognize SARS-CoV-2 SARS-CoV-2 receptor SARS-CoV-2 Spike RBD SARS-CoV-2 virus spike trimer the spike protein viral entry [DOI] 10.1038/s41594-020-0469-6 [Article Type] Research
Preliminary Virtual Screening Studies to Identify GRP78 Inhibitors Which May Interfere with SARS-CoV-2 InfectionCommunication Published on 2020-06-252022-10-28 Journal: Pharmaceuticals [Category] COVID-19, [키워드] addition Anti-SARS-CoV-2 Activity Antiviral antiviral activity approved drug binding domain Blood carried clinically Compound COVID-19 drug DrugBank Evidence expression Gene Expression GRP78 host cell surface identify indicated inhibit inhibitors molecular molecular docking molecular target mRNA level nucleotide binding domain patients performed Pneumonia predicted Protein Repurposed drugs Result retrieved SARS-CoV-2 Screening small molecule spike suggested targets treat Virtual screening was increased was performed [DOI] 10.3390/ph13060132 PMC 바로가기 [Article Type] Communication
An Off-the-Shelf Approach for the Production of Fc Fusion Proteins by Protein Trans -Splicing towards Generating a Lectibody In VitroArticle Published on 2020-06-032022-10-29 Journal: International Journal of Molecular Sciences [Category] Coronavirus, SARS, [키워드] antibodies antibody fragment approach binding binding domain binding site demonstrated domain domains Escherichia coli expression Fc functions fused fusion fusions Genetic HIV IgG Immunoglobulin G in vitro Infectious disease inhibit lectibody lectin N-terminus Organisms potential therapeutic agent produced producing Production Protein protein trans-splicing protein ligation SARS Coronavirus scytovirin segmental isotopic labeling split intein therapeutic Tran virus entry [DOI] 10.3390/ijms21114011 PMC 바로가기 [Article Type] Article
Essential Oils as Antiviral Agents, Potential of Essential Oils to Treat SARS-CoV-2 Infection: An In-Silico InvestigationArticle Published on 2020-05-122022-10-29 Journal: International Journal of Molecular Sciences [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome Antiviral antiviral agent Antiviral agents binding domain carried component Corona virus coronavirus COVID-19 COVID-19 symptoms docking docking score enzyme Essential essential oil essential oils hACE2 investigation Ligand M pro molecular docking molecular docking analysis normalized Oil pathogenic viruses Potential Protein Protein target RNA-dependent RNA polymerase SARS-CoV-2 SARS-CoV-2 main protease SARS-CoV-2 RdRp shown Spike protein target targets the SARS-CoV-2 unlikely virus [DOI] 10.3390/ijms21103426 PMC 바로가기 [Article Type] Article
Identification of a novel base J binding protein complex involved in RNA polymerase II transcription termination in trypanosomes트리파노솜에서 RNA 중합효소 II 전사 종결에 관여하는 새로운 염기 J 결합 단백질 복합체의 확인Research Article Published on 2020-02-212022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] Ablation addition African sleeping sickness Analysis base binding binding domain cause co-IP complex dephosphorylation derepression described DNA downstream genes Epigenetic eukaryotes expand expressed expression Factors gene cluster gene clusters Gene Expression Genome homolog Host Human humans identification identify immune evasion Interaction involved lead leads Leishmania mammalian mass spectrometry mechanism Modification mutant mutants organism Organisms organized phosphatase promoter Protein protein complex Protozoa Reciprocal recruitment regulate regulated Regulatory residue RNA RNA Polymerase II RNA-Seq RNAi RT-PCR shown significantly silent sleeping sickness specific proteins sucrose surface Surface glycoprotein termination thymine Transcription transcription start sites Trypanosoma Trypanosoma brucei Trypanosome upstream variant Variant Surface Glycoprotein Wdr82 yeast [DOI] 10.1371/journal.pgen.1008390 PMC 바로가기 [Article Type] Research Article
Evidence for the evolutionary steps leading to mecA -mediated β-lactam resistance in staphylococci포도상구균에서 mecA 매개 β-락탐 내성으로 이어지는 진화 단계에 대한 증거Research Article Published on 2017-04-102022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] accumulation acquisition adaptation Amino acid Analysis antibiotic Antibiotic resistance Antibiotics Author Bacteria Bacterial Bayesian analysis binding binding domain cell wall cell wall synthesis changes in chromosome Community core genome development domain driving epidemiologically Evolution exposure to food additives gene encoding Genetic genetic background genomes highlight homologue identify involved isolates livestock mechanism mechanisms methicillin methicillin-resistant Staphylococcus aureus Mortality MRSA mutation rate Mutation rates non-binding nucleotide occur occurred oxacillin Pandemics PBP penicillin phenotype Phylogenetic analysis precursor predicted profiles promoter promoters Protein representing resistance gene resource responsible S . aureus SCC selective sequence sequence alignments sequenced genome single-nucleotide polymorphism SNP species Spread Staphylococcus Staphylococcus aureus strain Substitution substitutions susceptibility variety was performed [DOI] 10.1371/journal.pgen.1006674 PMC 바로가기 [Article Type] Research Article
Amplification of TLO Mediator Subunit Genes Facilitate Filamentous Growth in Candida Spp .TLO 중재자 소단위 유전자의 증폭은 칸디다 종에서 필라멘트 성장을 촉진합니다.Research Article Published on 2016-10-142022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] Activation amplification amplitude ascomycete ascomycetes binding domain bloodstream infection Bloodstream infections Candida Candida albican Candida albicans candidiasis cause cell physiology complex death DNA domain Engineering ENhance environmental cues eukaryotic exhibit facilitated Filamentous fungal fungi gene family Genome genomes genomic growth hallmark individual Infection infections Interaction less mediator mortality rate multitude murine mutant Nuclear localization nuclear localization signal nucleus ortholog overexpressed overexpression pathogenicity phenotype Phenotypes phenotypic plasticity Protein provide reduced regulate subunit Support systemic Candidiasis systemic infection target targets The United States Transcription transcription factor transcription factors transcriptional transcriptional activation unique United States virulence [DOI] 10.1371/journal.pgen.1006373 PMC 바로가기 [Article Type] Research Article
Genetic drift of human coronavirus OC43 spike gene during adaptive evolutionArticle Published on 2015-06-222022-10-29 Journal: Scientific Reports [Category] Coronavirus, [키워드] adaptive Analysis binding binding domain characterized coronavirus CoV CoVs Critical dN/dS dominant Epidemic Evolution FIVE Genetic genetic drift Genotype HCoV-OC43 Health human coronavirus OC43 human population human populations majority mechanism N-terminal domain NTD OC43 persistence phylodynamic Phylogenetic Positive selection prevalent S gene S protein Seven spike gene Substitution sugar the S1 subunit [DOI] 10.1038/srep11451 PMC 바로가기 [Article Type] Article
Defining NELF-E RNA Binding in HIV-1 and Promoter-Proximal Pause RegionsHIV-1 및 프로모터-근위 일시 중지 영역에서 NELF-E RNA 결합 정의Research Article Published on 2014-01-162022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] a major addition analyzed association Author binding binding activity binding domain Biochemistry cellular claimed compared complex Consensus conserved contribute downstream Drosophila elongation elongation factor eukaryotic facilitate Factor functional Gene Genes Genome high affinity HIV-1 identify in addition to in vitro Interaction modulate molecular negative nuclear nucleotide nucleotides observé pause POLL promoter Protein protein coding gene protein coding genes Protein subunit Quantitative Recognition Region regulate regulated Regulatory required responsible RNA RNA Polymerase II RNA sequence RNA sequences sequence significant increase subunit Support targets thought transcribed Transcription transcription factor transcription factors transcription start site widespread [DOI] 10.1371/journal.pgen.1004090 PMC 바로가기 [Article Type] Research Article