Development of a Smartphone-Based Nanozyme-Linked Immunosorbent Assay for Quantitative Detection of SARS-CoV-2 Nucleocapsid Phosphoprotein in BloodMicrobiology Published on 2021-08-232022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] Antigen applied Blood China collected control sample coronavirus disease COVID-19 COVID-19 patient cross-reactivity detect detection development diagnosed diagnosis of COVID-19 ELISA enzyme-linked immunosorbent global pandemic immunoassay immunosorbent Novel coronavirus nucleocapsid Nucleocapsid phosphoprotein Phosphoprotein POCT device Pt@AuNPs required SARS-CoV-2 SARS-CoV-2 infected individuals SARS-CoV-2 RNA serum sample Smartphone viral nucleic acid virus [DOI] 10.3389/fmicb.2021.692831 PMC 바로가기 [Article Type] Microbiology
Safety and immunogenicity of the ChAdOx1 nCoV-19 (AZD1222) vaccine against SARS-CoV-2 in people living with and without HIV in South Africa: an interim analysis of a randomised, double-blind, placebo-controlled, phase 1B/2A trial남아프리카에 HIV가 있거나없는 사람들의 SARS-COV-2에 대한 Chadox1 NCOV-19 (AZD1222) 백신의 안전성 및 면역 원성 : 무작위, 이중 맹검, 위약 대조, Phase 1B/2A 시험의 중간 분석Clinical Trial Published on 2021-08-172022-08-13 Journal: The Lancet. HIV [Category] MERS, SARS, 변종, 임상, 진단, [키워드] 1:1 1B/2A trial 95% CI adverse event adverse events African Alanine alanine aminotranferase allocation concealment Antibody Response Antibody Units Antiretroviral therapy Asp614Gly wild-type AZD1222 B.1.351 baseline Beta Beta variant binding antibodies binding antibody unit booster booster response both groups ChAdOx1 ChAdOx1 nCov 19 ChAdOx1 nCoV-19 clinical COV005 criteria cross-reactive binding antibodies cross-reactive binding antibody cross-reactivity D614G demonstrated died domain dose double-blind elevated eligibility criteria enrolled enrolment Fever finding FIVE foundation full-length funding geometric mean geometric mean concentration GMC high responder high responders High-level binding antibody HIV HIV-negative hospital IgG responses immunogenicity in both groups increased risk individual individuals Innovation interim analysis Intervention less Medical Research Council Melinda Gate Melinda Gates n=36 nCoV neutralisation Neutralising activity outcome Pan participant Participants people with HIV person with HIV Placebo placebo-controlled plasma Primary immunogenicity analyses Primary immunogenicity analysis prime-boost regimen priming priming dose randomised Randomly reactogenicity receive Receptor-binding domain Registered registry responses responses against retained rising Safety SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 antigens Serious Adverse Events seronegative seropositive serum IgG Seven severe COVID-19 South South Africa titre Trial unlikely Vaccine vaccine dose Viral Viral load wild-type wild-type Wuhan-1 Asp614Gly with HIV [DOI] 10.1016/S2352-3018(21)00157-0 PMC 바로가기 [Article Type] Clinical Trial
Mass spectrometry-based proteomics in basic and translational research of SARS-CoV-2 coronavirus and its emerging mutantsReview Published on 2021-08-122022-11-01 Journal: Clinical Proteomics [Category] COVID-19, [키워드] acute respiratory syndrome anti-viral antibody assays Betacoronavirus clinical study Combination continuous monitoring coronavirus coronavirus disease COVID-19 cross-reactivity diagnostic Diseases Emerging mutations evaluate facilitate functional identify Immunity immunoassays immunoprecipitation information knowledge mass mass spectrometry molecular diagnostics mutant mutations pandemic Pandemics Perspective Population post-translational modifications Protein protein–protein interactions proteomic proteomics quantification rapid response Research RNA sequencing RT-PCR SARS-CoV-2 SARS-CoV-2 coronavirus sensitivity Serology diagnostics serology test specificity targeted proteomics translational Viral protein Viral RNA viruses while [DOI] 10.1186/s12014-021-09325-x PMC 바로가기 [Article Type] Review
Evaluation of accuracy, exclusivity, limit-of-detection and ease-of-use of LumiraDx™: An antigen-detecting point-of-care device for SARS-CoV-2LumiraDx™의 정확성, 독점성, 검출 한계 및 사용 편의성 평가: SARS-CoV-2용 항원 검출 현장 진료 장치Multicenter Study Published on 2021-08-122022-09-10 Journal: Infection [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, [키워드] 95% CI Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Ag-RDT Ag-RDTs analytical sensitivity Antigen-detecting diagnostics calculate Chain Reaction clinical sensitivity clinical specificity collected conducted coronavirus COVID-19 cross-reactivity diagnostic Diagnostic accuracy enrolled evaluated immunofluorescence individual investigated nasal nasopharyngeal pandemic participant pathogen Pathogens point-of-care polymerase chain polymerase chain reaction positive Rapid RDTs registration date registration number reporting symptom respiratory Result resulting reverse transcriptase Reverse transcriptase polymerase chain reaction RNA RNA copies RT-PCR SARS-CoV-2 SARS-CoV-2 detection SARS-COV-2 infection SARS-CoV2 Self-swab sensitivity Sensitivity and specificity severe acute respiratory syndrome Coronavirus supplementary material Swab Symptom symptomatic and asymptomatic Testing transcriptase Viral Viral load was increased [DOI] 10.1007/s15010-021-01681-y PMC 바로가기 [Article Type] Multicenter Study
Performance Evaluation of the MatMaCorp COVID-19 2SF Assay for the Detection of SARS-CoV-2 from Nasopharyngeal Swabs비인두 면봉에서 SARS-CoV-2 검출을 위한 MatMaCorp COVID-19 2SF 분석의 성능 평가Research Article Published on 2021-08-112022-09-10 Journal: Microbiology Spectrum [Category] MERS, SARS, 진단, 치료기술, [키워드] 95% CI 95% confidence interval Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplification Cepheid coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 test cross-reactivity detection Diagnosis diagnostic diagnostic testing evaluate evaluated help Inc. inherent Laboratory MatMaCorp molecular NAAT nasopharyngeal nucleic acid amplification testing offer pandemic Pathogens PCR-based testing performance platform point of care positive provide qualitative detection reagent reagents respiratory respiratory pathogen reverse transcription reverse transcription-PCR RT-PCR Salt Lake Salt Lake City SARS-CoV-2 Sensitivity and specificity severe acute respiratory syndrome Coronavirus shelf Solas 8 specificity specimen swabs tested unique were used [DOI] 10.1128/Spectrum.00083-21 PMC 바로가기 [Article Type] Research Article
Precision Response to the Rise of the SARS-CoV-2 B.1.1.7 Variant of Concern by Combining Novel PCR Assays and Genome Sequencing for Rapid Variant Detection and SurveillanceSARS-CoV-2의 부상에 대한 정밀 대응 B.1.1.7 신속한 변이 검출 및 감시를 위한 새로운 PCR 분석과 게놈 시퀀싱을 결합하여 우려 변이Research Article Published on 2021-08-112022-09-10 Journal: Microbiology Spectrum [Category] MERS, SARS, 변종, 치료기술, 치료제, [키워드] 95% CI Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 approach B.1.1.7 B.1.351 Canada cause coefficient concern coronavirus COVID-19 COVID-19 pandemic cross-reactivity demonstrated detect detection Escalation Evolution of SARS-CoV-2 faster Genome sequencing gold gold standard help high correlation identify immune response less limits Limits of detection Lineage Mutation N501Y N501Y mutation Negative predictive value novel P.1 Pathogens PCR PCR assay PCR assays Positive predictive value positive predictive values predictive values Prevent Rapid real-time reverse transcriptase respiratory respiratory pathogen response reverse transcriptase Sampling strategies sampling strategy SARS-CoV-2 SARS-CoV-2 variant sensitivities sensitivity Sequencing severe acute respiratory syndrome Coronavirus severe disease Specificities specificity spike gene strain Strains Surveillance susceptibility targets the SARS-CoV-2 Transmission variants variants of concern Variation virus VoC VOCs wild-type [DOI] 10.1128/Spectrum.00315-21 PMC 바로가기 [Article Type] Research Article
Coronavirus Pseudotypes for All Circulating Human Coronaviruses for Quantification of Cross-Neutralizing Antibody Responses교차 중화 항체 반응의 정량화를위한 모든 순환 인간 코로나 바이러스에 대한 코로나 바이러스 의사 타입Article Published on 2021-08-102022-09-01 Journal: Viruses [Category] MERS, SARS, 진단, [키워드] ACE2 antibody antibody cross-reactivity Antibody neutralization best Betacoronavirus breadth can be used Cell cell line cells CHO cell CHO cells Clinical outcome condition coronavirus COVID-19 COVID-19 disease COVID-2019 cross-reactivity disease Disease progression Effect Endemic envelope Haemagglutinin HCoV HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 HCoVs HEK293T HKU1 HUH7 cells Human human coronavirus Immunity immunopathology lentiviral MERS MERS-CoV natural infection neutralization Neutralizing NL63 Novel coronavirus novel coronavirus SARS-CoV-2 OC43 pre-existing immunity produced progression Protein Proteins provide pseudotype pseudotype virus pseudotype viruses Pseudotyped virus Research question response Safe SARS-CoV-1 SARS-CoV-2 seasonal coronavirus serological Seropositivity Seven surface proteins target cell target cells the SARS-CoV-2 titre TMPRSS2 TMPRSS2 expression transduced transfected with understanding vaccination viruses [DOI] 10.3390/v13081579 PMC 바로가기 [Article Type] Article
Comparison of SARS-CoV-2- and HCoV-Specific T Cell Response Using IFN-γ ELISpotArticle Published on 2021-08-102022-10-31 Journal: Diagnostics [Category] COVID-19, [키워드] acute respiratory syndrome addition Analysis Cell cellular immunity Cohort convalescent coronavirus Coronaviruses cross-reactivity detect detectable ELISPOT Endemic Evidence examined groups help herd immunity human endemic coronavirus IFN-γ ELISPOT Immunity Immunocompromised patients individual individuals Infection N-terminus neutralizing antibody no correlation performed Prevent Previous studies receive response SARS-CoV-2 SARS-CoV-2 vaccination Spearman stimulated T cell T cells T-cell Response the spike protein to define Vaccine Volunteer volunteers [DOI] 10.3390/diagnostics11081439 PMC 바로가기 [Article Type] Article
Interactions of Spike-RBD of SARS-CoV-2 and Platelet Factor 4: New Insights in the Etiopathogenesis of ThrombosisSARS-CoV-2의 Spike-RBD와 혈소판 인자 4의 상호 작용: 혈전증의 병인 발생에 대한 새로운 통찰력Communication Published on 2021-08-092022-09-12 Journal: International Journal of Molecular Sciences [Category] MERS, 진단, [키워드] ACE2 administration adverse event aggregation anti-spike antibodies antibodies antibody binding biochemical conditions contrary cross-reactivity develop diagnosed ELISA assays evaluated evidenced expression Factor Frequency heparin insight Interaction involved much higher New pathogenic Patient PF4 Platelet platelet factor 4 Platelets pro-thrombotic Protein RBD proteins recognize reported SARS-CoV-2 SARS-CoV2 severe COVID-19 similarities similarity specific antibodies specific antibody spike Spike protein Spike-RBD Structure structures syndrome the patient therapy These data thrombocytopenia thrombosis vaccination Vaccine [DOI] 10.3390/ijms22168562 PMC 바로가기 [Article Type] Communication
Poliovirus Vaccination Induces a Humoral Immune Response That Cross Reacts With SARS-CoV-2Medicine Published on 2021-08-032022-10-31 Journal: Frontiers in Medicine [Category] COVID-19, [키워드] adaptive Adaptive immunity age analyzed available data Cell culture childhood children correlation COVID-19 cross-reactivity cultures death rates demonstrated Effect effective enzyme-linked immunosorbent epidemiological evaluated humoral immune response Immunity immunization immunized individual induce Infection inhibited lack less median median age National outbreak pathogenic pediatric polio Poliovirus poliovirus vaccine Population positive correlation presenting Prevalence Protein protocol RdRP RdRp protein response Retrospective study RNA-dependent RNA polymerase SARS-CoV-2 SARS-COV-2 infection sera serum severity symptomatic cases target vaccination Vaccinations Vaccine development Vero Cell [DOI] 10.3389/fmed.2021.710010 PMC 바로가기 [Article Type] Medicine