Combination of a Sindbis-SARS-CoV-2 Spike Vaccine and αOX40 Antibody Elicits Protective Immunity Against SARS-CoV-2 Induced Disease and Potentiates Long-Term SARS-CoV-2-Specific Humoral and T-Cell Immunitysindbis-sars-cov-2 스파이크 백신과 αOX40 항체의 조합은 SARS-COV-2 유도 질환에 대한 보호 면역을 유도하고 장기 SARS-COV-2- 특이 적 유머 및 T- 세포 면역을 강화시킨다.Immunology Published on 2021-07-292022-08-31 Journal: Frontiers in Immunology [Category] SARS, 바이오마커, 변종, 진단, [키워드] αOX40 activated T cells Against alphavirus vaccine Analysis antibody approach can be used caused cellular coronavirus coronavirus SARS-CoV-2 COVID-19 pandemic COVID19 develop dose effective effective vaccine effector memory effort elicit elicited expressing functions generate hACE2 human ACE2 receptor immune protection immune response immunization immunize Immunophenotyping immunostimulatory induced Infection infection assay inhibit long-lasting neutralizing antibodies long-lasting neutralizing antibody lung tissue mice MOST mounted Neutralizing antibodies OX40 Pathogens platform profiles protective immunity Protein binding provided reprogramming RNA robust SARS-CoV-2 SARS-CoV-2 immunity SARS-CoV-2 spike protein SARS-CoV-2 vaccine sera Sindbis virus vaccine spike Spike protein synergistic combination SARS-CoV-2 vaccine strategy T cell T cells T-cell Response T-cell responses the SARS-CoV-2 the vaccine Transcriptome transgenic mice Vaccine vaccine platform variant variants [DOI] 10.3389/fimmu.2021.719077 PMC 바로가기 [Article Type] Immunology
Structural Modeling of the SARS-CoV-2 Spike/Human ACE2 Complex Interface can Identify High-Affinity Variants Associated with Increased TransmissibilitySARS-CoV-2 스파이크/인간 ACE2 복합 인터페이스의 구조 모델링은 전파율 증가와 관련된 고친화도 변이를 식별할 수 있습니다.Article Published on 2021-07-232022-09-11 Journal: Journal of Molecular Biology [Category] SARS, 변종, [키워드] ACE2 affinity Analysis antibody antibody resistance Applying binding binding affinities binding affinity changes in circulating Cluster Combination combinatorial mutations complex complexes COVID-19 pandemic determine E484K Effect flexible functional G476S generate hACE2 help human ACE2 human ACE2 receptor Increased Infection Interaction interface Intervention K31 K353 modeling mutant Mutation N501Y pathogenic Point mutation predict predicted Protein Protein binding public health measure Region reveal S477N SARS-CoV-2 SARS-CoV-2 spike protein screening method sequence spike spike mutations Spike protein Structural analysis structural modeling susceptibility susceptibility to infection the SARS-CoV-2 Transmissibility transmissible variants transmissible variants. triggered variant variants Variation viral strains viral variant wild-type [DOI] 10.1016/j.jmb.2021.167051 PMC 바로가기 [Article Type] Article
hACE2 Fc-neutralization antibody cocktail provides synergistic protection against SARS-CoV-2 and its spike RBD variantsCorrespondence Published on 2021-07-202022-10-31 Journal: Cell Discovery [Category] COVID-19, [키워드] antibody cocktail Cell Biology hACE2 immunology provide RBD variant SARS-CoV-2 synergistic [DOI] 10.1038/s41421-021-00293-y PMC 바로가기 [Article Type] Correspondence
Inhalable nanocatchers for SARS-CoV-2 inhibitionSARS-CoV-2 억제를 위한 흡입 가능한 나노캐처422 Published on 2021-07-202022-09-10 Journal: Proceedings of the National Academy of Sciences of [Category] SARS, 변종, [키워드] ACE2 acute respiratory syndrome angiotensin binding binding affinity caused Cell cellular membrane Clinical use coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 epidemic D614G variant develop Efficacy exhibit exhibited exhibiting expressing hACE2 hACE2-containing nanocatchers Health Health crisis host cell host cells human Angiotensin-converting enzyme human cell human cells Hyaluronic acid Infection inhalation inhibit SARS-CoV-2 lead lung lyophilized formulation mucoadhesive mutated mutations neutralization ability organ pandemic Prevent PROTECT Protein pseudovirus pseudoviruses raised receptors respiratory retention SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 variant SARS-CoV-2 variants Severe acute respiratory syndrome Side effects Significance significantly spike glycoprotein strain transmission of SARS-CoV-2 Treatment Vaccine Viral Viral mutations virus virus inhibitor viruses wild-type SARS-CoV-2 [DOI] 10.1073/pnas.2102957118 PMC 바로가기 [Article Type] 422
Metagenomic identification of a new sarbecovirus from horseshoe bats in EuropeArticle Published on 2021-07-192022-10-04 Journal: Scientific Reports [Category] SARS, 변종, 유전자 메커니즘, [키워드] Amino acid Asian bat bats binding ability Co-infection COVID-19 pandemic Critical distribution Europe extreme hACE2 highlight Homologous recombination homology Host Mutation Preventing Receptor binding domain receptor binding motif Recombination sarbecovirus SARS-CoV SARS-CoV-2 sequenced the spike protein thought transmission of SARS-CoV-2 unlikely vaccination Viral reservoirs virus while zoonotic [DOI] 10.1038/s41598-021-94011-z PMC 바로가기 [Article Type] Article
Inhibition of SARS-CoV-2 pseudovirus invasion by ACE2 protecting and Spike neutralizing peptides: An alternative approach to COVID19 prevention and therapyResearch Paper Published on 2021-07-132022-10-29 Journal: International Journal of Biological Sciences [Category] COVID-19, SARS, [키워드] ACE2 addition administration antibody antibody resistance Antibody-dependent enhancement approach Cell cells Cocktail therapy Combination COVID-19 COVID19 docking effective hACE2 host cell host cell membrane Impact in vitro assays inhibited inhibition Interaction intranasal Invasion neutralization Neutralizing neutralizing activities peptide peptides potential risk predicted pseudotype pseudovirus S-protein Safe SARS-CoV-2 SARS-CoV-2 pseudovirus spike Spike protein spike-protein therapeutic therapy Toxicity transgenic mice [DOI] 10.7150/ijbs.61476 PMC 바로가기 [Article Type] Research Paper
Tagged extracellular vesicles with the RBD of the viral spike protein for delivery of antiviral agents against SARS-COV-2 infectionSARS-COV-2 감염에 대한 항바이러스제 전달을 위한 바이러스 스파이크 단백질의 RBD로 태그가 지정된 세포외 소포Article Published on 2021-07-102022-09-11 Journal: Journal of controlled release : official journal o [Category] SARS, 치료제, [키워드] accumulate ACE2 ACE2 receptor antiviral agent Antiviral agents approach binding domain Cell cellular cellular entry develop domain effective encapsulate endocytosed Express Extracellular extracellular vesicle Extracellular vesicles fusion hACE2 highlight in vivo Infection Interaction lung tissue lung tissues membrane membrane protein Membrane proteins modify molecular provide pseudovirus infection RBD Receptor binding domain receptor binding domain (RBD) Receptor-binding domain recognize required Safe SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 pseudovirus siRNA siRNAs specific tissue specific tissues Spike protein spread of COVID-19 suppress Tagged target cells target tissue target tissues targeting the RBD the receptor-binding domain therapeutic potential Therapeutics tissue Transgenic mouse viral spike protein [DOI] 10.1016/j.jconrel.2021.05.049 PMC 바로가기 [Article Type] Article
The molecular basis for SARS-CoV-2 binding to dog ACE2Article Published on 2021-07-072022-10-04 Journal: Nature Communications [Category] SARS, 변종, [키워드] ACE2 angiotensin-converting enzyme 2 binding cells complex Contact COVID-19 pandemic Cross-species transmission domain hACE2 highlight host range human ACE2 Hydrogen bond increase infect infected with SARS-CoV-2 molecular basis MONITOR Mutation N501Y mutation potential transmission Protein provide pseudotyped RBD receptor Receptor binding domain residues reveal Salt Bridge SARS-CoV-2 SARS-CoV-2 mutation Spike protein Spread the binding affinity the RBD the SARS-CoV-2 viral infection virus [DOI] 10.1038/s41467-021-24326-y PMC 바로가기 [Article Type] Article
A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutationsResearch article Published on 2021-07-072022-10-05 Journal: Cell Reports [Category] SARS, 변종, [키워드] ACE2 ACE2-RBD anti-SARS-CoV-2 antibodies antibody antibody fragment bind binding blocked cause clinical trial complex convalescent COVID-19 patient coronavirus coronavirus disease COVID-19 COVID-19 patient demonstrated develop disease Efficacy Fab hACE2 human Angiotensin-converting enzyme human IgG1 IC50 immune library in vivo in vivo neutralization live SARS-CoV-2 mice moderate Neutralization assay Neutralizing neutralizing antibody novel Betacoronavirus phage display phase RBD RBD mutation RBD mutations recombinant antibody SARS-CoV-2 SARS-CoV-2 neutralizing antibody SARS-CoV-2 variants SARS-CoV-2-RBD scFv selected severe COVID-19 severe pneumonia Spike protein the receptor-binding domain the spike protein Treatment [DOI] 10.1016/j.celrep.2021.109433 [Article Type] Research article
SARS-CoV-2 Infects Endothelial Cells In Vivo and In VitroSARS-CoV-2는 생체 내 및 시험관 내 내피 세포를 감염시킵니다Cellular and Infection Microbiology Published on 2021-07-062022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] MERS, SARS, 치료기술, [키워드] ACE-2 activated Ad5-hACE2 addition Adenovirus Affect animal models aorta ring cardiovascular complication cardiovascular complications cause CD31 CD34 cellular activation characterized Comparative analysis contribute cytokine Cytokine storm demonstrated died dysfunction edema electron microscopic analysis Endothelial cell endothelial cell infection endothelial cells endotheliopathy Ex vivo exposure to expressing focus hACE2 hemorrhage immunostaining in vivo infect infecting Inflammatory inflammatory cascade intranasal KRAS labeling Localization lung Lung pathology lung tissue Lungs marker mice microscopic analysis non-human primate non-human primates other organs pathway Patient Pneumocytes positive Protein RNA RNA sequencing data SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 protein SARS-CoV-2 proteins severe COVID-19 signaling pathway thrombosis upregulated vascular endothelial cell vascular inflammation vascular permeability Viral viral particle Viral particles [DOI] 10.3389/fcimb.2021.701278 PMC 바로가기 [Article Type] Cellular and Infection Microbiology