Analysis of the Genome and Transcriptome of Cryptococcus neoformans var. grubii Reveals Complex RNA Expression and Microevolution Leading to Virulence AttenuationCryptococcus neoformans var.의 게놈 및 전사체 분석 grubii, 복잡한 RNA 발현 및 독성 약화로 이어지는 소진화를 밝혀냄Research Article Published on 2014-04-172022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] a major addition alternative polyadenylation Analysis analyzed annotation attenuation automated C. neoforman CDS chromosomal coding coding DNA sequence coding DNA sequences Comparative analysis Cryptococcus Cryptococcus neoforman Cryptococcus neoformans death Efficiency examination expressed gene expressed genes feature fungi Gene Expression Genes Genetic genetics Genome genome sequence genomic studies genomic study human pathogen identify Immunocompromised Immunocompromised patients in vitro culturing indicate intron introns involved isolate isolates Laboratory leading manual curation meningoencephalitis microevolution microevolutionary miscRNA miscRNAs molecular motif Mutation Non-coding RNAs occur opportunistic pathogen pathogen pathogenic performed phenotypic phenotypic and genome re-sequencing analysis polyadenylation site polyadenylation sites provide Region Regulation Replication representing responses responsible resulting RNA RNA-Seq-based analysis sequence sequenced sequences Sequencing serotype Spectrum strain Stress stress responses Transcription Transcriptome untranslated regions UTRs VAR variant variety virulence yeast [DOI] 10.1371/journal.pgen.1004261 PMC 바로가기 [Article Type] Research Article
Respiratory viruses within homeless shelters in Marseille, FranceShort Report Published on 2014-02-052022-10-27 Journal: BMC Research Notes [Category] Coronavirus, [키워드] 229E affecting asymptomatic infections asymptomatic patient clinical examination collected Community coronavirus described Epidemic finding found France high risk Homeless persons Homeless population homeless shelter HRV Human human coronavirus OC43 included Infected Influenza virus investigated isolates Local Marseille métapneumovirus MOST nasal NO outbreak Patient positive swab predominant Prevalence reported respiratory Respiratory disease respiratory symptom respiratory symptoms respiratory syncytial virus Respiratory tract disease respiratory virus rhinovirus RT-PCR detection Seasonal influenza specimen Swab the patient virus was performed [DOI] 10.1186/1756-0500-7-81 PMC 바로가기 [Article Type] Short Report
High Risk Population Isolate Reveals Low Frequency Variants Predisposing to Intracranial Aneurysms고위험군 분리가 두개내 동맥류에 걸리기 쉬운 저주파 변이를 나타냅니다Research Article Published on 2014-01-302022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] Aneurysm arteries Artery association Author balloon bleeding bottleneck events Brain Case-control cause compared complex complex diseases contribute Control controls develop different disease effect size effect sizes European population events explain explained Families Finn Finnish Finns FIVE fraction frequencies Frequency genetic background Genetic variant Genetic variation genotype imputation GWAS hemorrhage heritability identify incidence Inconclusive Intracranial intracranial aneurysms isolate isolates knowledge lead life-threatening loci locus low frequency MAF matched controls Netherland Netherlands Patient Population populations rare variant Rare variants related replicated rupture saccular stroke Study design Subarachnoid subarachnoid hemorrhage susceptibility tested utility variant variants [DOI] 10.1371/journal.pgen.1004134 PMC 바로가기 [Article Type] Research Article
A SYBR Green 1-based in vitro test of susceptibility of Ghanaian Plasmodium falciparum clinical isolates to a panel of anti-malarial drugs가나의 Plasmodium falciparum 임상 분리주에 대한 항말라리아 약물 패널에 대한 감수성을 평가하는 SYBR Green 1 기반의 인 비트로 테스트Research Published on 2013-12-172024-08-13 Journal: Malaria Journal [Category] 말라리아, [키워드] in vitro inhibition isolates Plasmodium falciparum susceptibility [DOI] 10.1186/1475-2875-12-450 PMC 바로가기 [Article Type] Research
Combining Quantitative Genetic Footprinting and Trait Enrichment Analysis to Identify Fitness Determinants of a Bacterial Pathogen정량적 유전 발자국과 특성 강화 분석을 결합하여 세균성 병원체의 적합성 결정 요인 식별Research Article Published on 2013-08-222022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] a major abundance Affect Algorithm Antibiotic resistance approach approaches array assortment Author Bacterial bacterial gene bacterial genes benefit bioinformatics candidate gene changes in Combination conjunction conserved dataset deep sequencing deleted determinant determinants Determining diagnostic dissect Diversity DNA binding E. coli effective effort enrichment Escherichia Escherichia coli Factor fitness Follow-up analysis Footprinting found Gene Genes Genetic Genome genomes highest highlight homologue homologues Host hypothetical identify increasingly independent Infection infections insertion investigator investigators isolates Meningitis molecular mouse model Mutagenesis mutant novel observation observations pathogen Pathogenesis pathogenic pathogenic strain pathogenic strains Pathogens phenotypic phenotypic variation phylogenetic distribution promote Quantitative reported required Research resource saturated Sepsis Sequencing shown strain Strains suggested therapeutic therapeutic targets Therapies Tn-seq to define trait transcriptional repressor transposon urinary tract infections variant Variation virulence virulence gene widespread zebrafish [DOI] 10.1371/journal.pgen.1003716 PMC 바로가기 [Article Type] Research Article
High-Resolution Transcriptome Maps Reveal Strain-Specific Regulatory Features of Multiple Campylobacter jejuni Isolates고해상도 전사체 지도는 여러 Campylobacter jejuni 분리주의 균주 특이적 조절 기능을 보여줍니다Research Article Published on 2013-05-162022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] adaptation addition Analysis analyzed approach Author automated Bacterial bioinformatics Campylobacter Campylobacter jejuni compared consequence conservation conserved contribute CRISPR different DNA ecological niche ecological niches eukaryote eukaryotes facilitate feature functional gastroenteritis Gene Gene Expression Gene regulation Genetic Genome genome sequence Genome sequences genomic variation genomic variations genotypic genotypic variations growth Host hosts humans immune system IMPROVE include integration isolate isolates lead Map maturation modulate Multiple organism Organisms Pathogens pathway phenotypic phenotypic diversity phenotypic variation promoter promoter regions promoters provide Regulation related reveal RNA RNA sequencing RNase similarities similarity small RNA SNP sRNA strain Strains stress conditions time-consuming transcribed transcriptional Transcriptome transcriptomes TSS unique upstream Variation whole-genome [DOI] 10.1371/journal.pgen.1003495 PMC 바로가기 [Article Type] Research Article
Human Monoclonal Antibodies against Highly Conserved HR1 and HR2 Domains of the SARS-CoV Spike Protein Are More Broadly NeutralizingResearch Article Published on 2012-11-212022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] Amgen antibody binding conserved convalescent patient different ectodomain effective expressing glycoprotein Human Human monoclonal antibody immune implication Infected isolates monoclonal antibody neutralize Neutralizing Patient produced Pseudotyped virus Receptor binding domain Region respiratory S protein S1 domain S2 domain SARS-CoV SARS-CoV spike sequence sera severe shown Subunit vaccine the S protein therapeutic therapy Treatment variant viruses [DOI] 10.1371/journal.pone.0050366 PMC 바로가기 [Article Type] Research Article
The Three Faces of Riboviral Spontaneous Mutation: Spectrum, Mode of Genome Replication, and Mutation Rate리보바이러스 자연 돌연변이의 세 가지 측면: 스펙트럼, 게놈 복제 모드 및 돌연변이 비율Research Article Published on 2012-07-262022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] ameliorated Analysis animals antiviral drugs Author average bacteriophage bacteriophages base substitution base substitutions characterization concerning detected disease Diseases DNA replication Escherichia coli exceptional face Frequency gene encoding generate genome replication growth Host hypermutation in vivo indels infecting Infection information investigated isolates iterative linear linear mode magnitude mammalian mode Mutagenesis mutant Mutation mutation rate mutational spectrum mutations pathogen Pathogens phage plants Plasmid Population produced Protein public health Rate reduce relative frequencies relative frequency Replication reported reporter resistance mutations RNA Sequencing analysis Signaling Spectrum Spontaneous spontaneous mutation subject these viruses trans transversion urgency viral disease virus viruses was used [DOI] 10.1371/journal.pgen.1002832 PMC 바로가기 [Article Type] Research Article
Comparative Pathogenesis of Three Human and Zoonotic SARS-CoV Strains in Cynomolgus MacaquesResearch Article Published on 2011-04-202022-10-28 Journal: PLoS ONE [Category] Coronavirus, Fulltext, 조류인플루엔자, [키워드] acute respiratory syndrome against SARS-CoV change characterized Comparative compared Critical disease cynomolgus macaque Disease model Efficacy Epidemic exacerbated function Gene Heterologous histopathological Host host gene host response Human infect Infected Infection interferon signaling isolates macaque majority Mild pathogen pathogenicity pathway Pathways Prevent radiological regulated Replication SARS-CoV significant difference strain Strains subsequent therapeutic strategy Transmission Vaccine while zoonotic [DOI] 10.1371/journal.pone.0018558 PMC 바로가기 [Article Type] Research Article
Identification and Characterization of Three Novel Small Interference RNAs That Effectively Down-Regulate the Isolated Nucleocapsid Gene Expression of SARS CoronavirusArticle Published on 2011-02-112022-10-30 Journal: Molecules [Category] Coronavirus, [키워드] analyzed Apoptosis cells characterization coding sequence conserved coronavirus different eGFP Evidence expression found function Gene Expression Host host cell identification IFNβ inhibitory effect innate immune responses Interference interferon Isolated isolates M gene N gene non-coding RNA novel Nucleocapsid gene nucleotide deletion post-transcriptionally Proinflammatory cytokine Protein reduce Region RNA RT-PCR SARS-CoV sequence sequence information significantly siRNA Substitution thought up-regulate [DOI] 10.3390/molecules16021544 PMC 바로가기 [Article Type] Article