Nanobodies Protecting From Lethal SARS-CoV-2 Infection Target Receptor Binding Epitopes Preserved in Virus Variants Other Than Omicron치명적인 SARS-CoV-2 감염 표적 수용체 결합 에피토프로부터 보호하는 나노바디는 오미크론 이외의 바이러스 변이체에 보존되어 있습니다Article Published on 2022-04-252022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), SARS, 변종, [키워드] affinity antibody binding binding epitope binding mode blocked cell cultures cell entry cell entry receptor competition coronavirus COVID-19 COVID-19 pandemic cryo Cryo-electron microscopy electron microscopy Epitopes fusion glycoprotein groups hACE2 heavy chain human ACE2 humanized immune Immune escape immunized lethal mice Microscopy mono MOST nanobodies nanobody neutralization neutralized Neutralizing Neutralizing antibodies neutralizing antibody nine omicron Other Prevent prevented progression Protecting pseudotyped RBD RBD binding RBM receptor receptor binding motif Receptor-binding domain S protein SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 RBD SARS-CoV-2 variant SARS-CoV-2 variants selected target the receptor-binding domain the S protein therapeutic potential Therapeutics Vaccine variant variants of concern virus [DOI] 10.3389/fimmu.2022.863831 PMC 바로가기 [Article Type] Article
Hybrid Proteins with Short Conformational Epitopes of the Receptor-Binding Domain of SARS-CoV-2 Spike Protein Promote Production of Virus-Neutralizing Antibodies When Used for ImmunizationArticle Published on 2022-04-082023-07-01 Journal: Biochemistry. Biokhimiia [Category] COVID19(2023년), SARS, [키워드] Aldolase epitope epitope vaccine RBD RBM S protein SARS-CoV-2 [DOI] 10.1134/S0006297922040022 PMC 바로가기 [Article Type] Article
Recombinant VLPs empower RBM peptides showing no immunogenicity in native SARS-COV-2 protein to elicit a robust neutralizing antibody response재조합 VLP는 천연 SARS-COV-2 단백질에서 면역원성을 나타내지 않는 RBM 펩타이드를 강화하여 강력한 중화 항체 반응을 이끌어냅니다.Article Published on 2022-04-012022-09-11 Journal: Nanomedicine : nanotechnology, biology, and medici [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus affinity Antigen antigenicity Antisera candidate carrier protein core antigen coronavirus demonstrated effective delivery elicit epitope Epitopes HBcAg immunization in vitro inactivated indicate KLH linear mice motif Multi-epitope vaccine Neutralizing activity neutralizing antibody Neutralizing antibody response New overlapping Particle peptide peptide epitope peptides Protein RBM reactivity receptor binding motif receptor-binding motif Receptor-binding motif (RBM) recombinant respiratory robust S1 protein severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Vaccine variants virus virus infection virus mutation Virus-like particle virus-like particles Virus-like particles (VLPs) VLP VLPs [DOI] 10.1016/j.nano.2022.102527 PMC 바로가기 [Article Type] Article
Mutations of Omicron Variant at the Interface of the Receptor Domain Motif and Human Angiotensin-Converting Enzyme-2수용체 도메인 모티프와 인간 안지오텐신 전환 효소-2의 인터페이스에서 오미크론 변이체의 돌연변이Article Published on 2022-03-062022-09-10 Journal: International Journal of Molecular Sciences [Category] MERS, SARS, 변종, [키워드] AABP unit ACE2 Amino acid Analysis angiotensin angiotensin-converting enzyme-2 Anxiety atomic benefit binding bonding calculated caused changes in complexity in shape and volume components computational modeling contribute evade explain facilitate Human human Angiotensin-converting enzyme immune defense in-depth analysis indicate Interaction interface mitigate Mutation observation omicron Omicron variant partial charge positive RBM receptor binding motif reveal SARS-CoV-2 shape significant changes in significant increase Spike protein Spread the spike protein Vaccines variant variant of SARS-CoV-2 virial transmission [DOI] 10.3390/ijms23052870 PMC 바로가기 [Article Type] Article
Risk-Based Monitoring in Clinical Trials: Increased Adoption Throughout 2020임상 시험의 위험 기반 모니터링: 2020년 전반에 걸쳐 채택 증가Analytical Report Published on 2022-03-022022-09-10 Journal: Therapeutic innovation & regulatory science [Category] MERS, 임상, [키워드] ACRO adoption clinical Clinical research clinical trial clinical trials component COVID-19 pandemic Data collection data integrity DCT event FIVE focus greater implementation Increased management monitoring organization percentage percentages RBM reduced reductions reductions in reported representing Trial trials [DOI] 10.1007/s43441-022-00387-z PMC 바로가기 [Article Type] Analytical Report
Structural basis for SARS-CoV-2 Delta variant recognition of ACE2 receptor and broadly neutralizing antibodiesACE2 수용체 및 광범위 중화 항체의 SARS-CoV-2 Delta 변이체 인식을 위한 구조적 기반Article Published on 2022-02-152022-09-10 Journal: Nature Communications [Category] COVID19(2023년), MERS, SARS, 변종, 유전자 메커니즘, 진단, [키워드] ACE2 ACE2 binding ACE2 receptor affinity Antibody neutralization antigenic binding circulating complex conformational conformational state contribute cryo-EM Cryo-EM analysis cryo-EM structure cryo-EM structures Cryoelectron microscopy Delta delta variant destabilized dominant effective Engagement FIVE identify immune induce Interaction lead mAbs mechanism Mutation Neutralizing neutralizing antibody pathogenicity RBD RBM receptor SARS-CoV-2 sensitivity shedding Spread T478K tested Transmission Viral proteins [DOI] 10.1038/s41467-022-28528-w PMC 바로가기 [Article Type] Article
Clinical and antibody characteristics reveal diverse signatures of severe and non-severe SARS-CoV-2 patients임상 및 항체 특성은 중증 및 비중증 SARS-CoV-2 환자의 다양한 특징을 나타냅니다.Research Article Published on 2022-02-022022-09-10 Journal: Infectious Diseases of Poverty [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, 치료제, [키워드] ACE2 adenosine adenosine deaminase analyzed antibodies antibody Antibody detection Antibody Response Antibody responses benefit bile acid binding C-reactive protein Characteristics clinical Clinical features clinical information collected contribute correlated COVID-19 COVID-19 case COVID-19 pandemic COVID-19 patient CTD cytokine Cytokines D-dimer domain domains elevated FcγRIIb Fisher’s exact test future therapeutic Ig isotypes IgG subclasses IgG1 IgG3 IL-6 IL-8 immune response investigated IP-10 LDH lymphocyte Lymphocyte count Mann–Whitney U test MCP-1 MCP-3 MIG neutrophil neutrophil count non-severe group NTD Patient patients preventive measure preventive measures procalcitonin Prognosis provide RBD RBD-ACE2 RBM receptors Result SARS-CoV-2 SARS-CoV-2 patient SARS-CoV-2 spike protein serum sample serum samples severe group Severe patient significantly specificity Spike protein supplementary material Treatment [DOI] 10.1186/s40249-022-00940-w PMC 바로가기 [Article Type] Research Article
From Free Binding Energy Calculations of SARS-CoV-2—Receptor Interactions to Cellular Immune ResponsesSARS-CoV-2의 자유 결합 에너지 계산 - 수용체 상호 작용에서 세포 면역 반응까지Brief Report Published on 2022-02-022022-09-10 Journal: Medicina [Category] COVID19(2023년), MERS, SARS, 변종, [키워드] angiotensin angiotensin converting enzyme Antigen assumed binding calculation change clinically computational analysis Computational biology danger signal danger signals disease severity energy enzyme expected Free free energy Free energy calculation Free energy calculations hACE2 HLA type Host human angiotensin converting enzyme 2 human leukocyte antigen immune responses infected cell Infection inflammatory immune responses Interaction leukocyte Lower respiratory tract molecular Occurrence omicron partial immunity pathogenicity Positive selection predicted produced RBM receptor Receptor binding Receptor binding domain receptor interaction reduced required respiratory response resulting SARS-CoV-2 SARS-CoV-2 Omicron SARS-CoV-2 spike protein Spike protein trade-off Transmissibility upper respiratory tract Vaccinations variants variants of concern virus viruses VOCs wild type [DOI] 10.3390/medicina58020226 PMC 바로가기 [Article Type] Brief Report
A scalable and highly immunogenic virus-like particle-based vaccine against SARS-CoV-2SARS-CoV-2에 대한 확장 가능하고 면역원성이 높은 바이러스 유사 입자 기반 백신Article Published on 2022-01-012022-09-11 Journal: Allergy [Category] SARS, 변종, 유전자 메커니즘, 진단, [키워드] added addition antibody Antibody Response Antibody responses Bacterial booster booster injections candidate vaccine caused centrifugation classical convalescent COVID-19 cross-reactive dose effective hand human sera hygiene immunogenic immunologically in some induce Injections long term Masks mice mutant Neutralizing Pandemics purification RBD RBM receptor-binding motif RNA SARS-CoV-2 stability the SARS-CoV-2 the vaccine Vaccine vaccine candidate Vaccines variants variants of concern virus virus-like particles wearing masks while yield [DOI] 10.1111/all.15080 PMC 바로가기 [Article Type] Article
Peptides derived from the SARS-CoV-2 receptor binding motif bind to ACE2 but do not block ACE2-mediated host cell entry or pro-inflammatory cytokine inductionResearch Article Published on 2021-11-182022-10-28 Journal: PLoS ONE [Category] COVID-19, [키워드] ACE2 angiotensin-converting enzyme 2 antibodies Antiviral antiviral activity approach binding binding affinity cell entry Contact crystal structure demonstrated Effect experiment expressing glycoprotein high affinity host cell human cell Inflammation inhibiting inhibitor Interaction linear majority membrane nanobody neutralisation assay peptide peptides pro-inflammatory cytokine RBM Receptor binding domain receptor binding motif residue responsible S RBD SARS-CoV-2 SARS-CoV-2 viral selected sequence soluble ACE2 Spike protein suggested suppress the S1 subunit the SARS-CoV-2 the spike protein to define trimer viral cell viral spike viral spike protein virulence [DOI] 10.1371/journal.pone.0260283 PMC 바로가기 [Article Type] Research Article