Ultrastructural insight into SARS-CoV-2 entry and budding in human airway epithelium사람의 기도 상피에서 SARS-CoV-2 진입 및 발아에 대한 미세구조적 통찰력Article Published on 2022-03-252022-09-11 Journal: Nature Communications [Category] MERS, SARS, 변종, 진단, 치료법, [키워드] ACE2 airway epithelium B.1.1.7 B.1.1.7 variant budding cilia ciliary ciliated ciliated cell ciliated cells Concentration direct fusion envelope epithelial cells examined excluded explain Extracellular Genome goblet host cells Human airway epithelium Intact isolate isolates mechanism membrane membranes microvilli nucleocapsid nucleocapsids periphery plasma membrane profile profiles S glycoprotein SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 infected cell SARS-CoV-2 infected cells TMPRSS2 tomography transmission electron microscopy Viral viral entry viral membrane virion virions virus [DOI] 10.1038/s41467-022-29255-y PMC 바로가기 [Article Type] Article
Angiotensin-Converting Enzyme 2 (ACE2), Transmembrane Peptidase Serine 2 (TMPRSS2), and Furin Expression Increases in the Lungs of Patients with Idiopathic Pulmonary Fibrosis (IPF) and Lymphangioleiomyomatosis (LAM): Implications for SARS-CoV-2 (COVID-19) InfectionsArticle Published on 2022-01-312022-10-30 Journal: Journal of Clinical Medicine [Category] COVID-19, [키워드] ACE2 ACE2 expression Actin Affect airway epithelium alveolar Cohort COPD COVID-19 driving elevated expression Factor furin growth factor healthy control higher risk idiopathic immunostained increase increase in interstitial Interstitial lung disease IPF knowledge LAM lung tissue Lymphangioleiomyomatosis marker Patient patients patients with ILD patients with IPF peptidase percentage positive correlation pulmonary pulmonary fibrosis reported risk SAE SARS-CoV-2 SARS-COV-2 infection secreted Severe COVID-19 Infection significantly smoker smoking TGF-β1 tissue TMPRSS2 upregulated was measured [DOI] 10.3390/jcm11030777 PMC 바로가기 [Article Type] Article
Airway Delivery of Hydrogel-Encapsulated Niclosamide for the Treatment of Inflammatory Airway DiseaseArticle Published on 2022-01-192022-10-29 Journal: International Journal of Molecular Sciences [Category] Coronavirus, [키워드] activated administration Affect airway airway epithelial cell airway epithelium anthelminthic applied Asthma asthmatic Calu-3 cells chronic obstructive circumvent CLCA1 clearance comparable Compound COVID-19 delivery disease drugs Effect effective encapsulation Ex vivo examined explained fibrosis glycol human airway epithelial hydrospheres in vitro in vivo Inflammatory inflammatory airway disease Influenza inhibit inhibited inhibitory effect intratracheal instillation Local mice Nanospheres niclosamide pro-inflammatory problems proinflammatory pulmonary disease repurposing respiratory syncytial virus SARS-CoV-2 secretion Side effects TMEM16A TMEM16F Trachea tracheas Treatment viral respiratory infections were assessed while [DOI] 10.3390/ijms23031085 PMC 바로가기 [Article Type] Article
Asthma Associated Cytokines Regulate the Expression of SARS-CoV-2 Receptor ACE2 in the Lung Tissue of Asthmatic PatientsImmunology Published on 2022-01-172022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, [키워드] ACE2 ACE2 expression Affect age airway epithelium association Asthma asthmatic Asthmatic patients biopsies Cohort Corticosteroid COVID-19 cytokine Cytokines data set determine differential expression evaluate expression expression level expression of SARS-CoV-2 Factor fibroblast Gender Gene Expression groups healthy controls IFN-γ IL-13 IL-17 IL-19 IL-4 IL-8 increase increased expression Inflammation Inflammatory cytokines less lower expression lung Lung inflammation lung tissue moderate receptors regulate Regulatory risk risk factor SARS-CoV-2 SARS-CoV-2 entry SARS-COV-2 infection SARS-CoV-2 receptor severity significantly sputum subjects susceptibility the patient tissue inflammation TMPRSS2 TMPRSS2 expression TMPRSS2 expression level Treatment Type upregulated were used [DOI] 10.3389/fimmu.2021.796094 PMC 바로가기 [Article Type] Immunology
Deciphering Respiratory-Virus-Associated Interferon Signaling in COPD Airway EpitheliumReview Published on 2022-01-132022-10-30 Journal: Medicina [Category] COVID-19, [키워드] accelerated activate Activation adaptive affecting airway epithelial cell airway epithelial cells airway epithelium Antiviral antiviral response antiviral therapy cascade characterized contribute COPD death Defense Deterioration disease severity effective Epidemic epithelium exhibit facilitate healthy Hospitalization Host IFN IFN signaling Immune homeostasis Immunity impairment increased risk Inflammatory mediators Inhaled Interaction interferon signaling Lower respiratory tract lung lung disorder Lung function mechanism mechanisms Mortality pathogen Patient patients pulmonary inflammation reduce Research respiratory respiratory virus response resulting secondary bacterial infection secreting severe disease Signaling subsequent susceptibility susceptible the interferon therapeutic intervention understanding viral infection virus virus clearance [DOI] 10.3390/medicina58010121 PMC 바로가기 [Article Type] Review
The Betacoronavirus PHEV Replicates and Disrupts the Respiratory Epithelia and Upregulates Key Pattern Recognition Receptor Genes and Downstream Mediators, Including IL-8 and IFN-λResearch Article Published on 2021-12-222022-10-30 Journal: mSphere [Category] Coronavirus, [키워드] Air-liquid interface airway epithelium ALI-REC Alpha Antiviral basal cells Betacoronavirus CCL2 CCL5 Cell culture cell cultures cells change chemokine chemokines ciliated cells coronavirus Culture CXCL10 CXCL8 Cytokines cytopathic disease Donor driving epithelia epithelial lining evaluated expression expression level Gene Expression goblet cells homeostasis IFN-λ1 IFN-stimulated gene IFN-λ IFN-λ1 IL-1β IL-6 IL-8 in vitro in vivo Including Increased infect Infection innate immune gene innate immune response Innate immunity innate response interleukin-1β investigated modulation molecular mechanism morphologically Necrosis Neonatal neurotropic OAS1 outcome pattern PHEV primary site Proinflammatory cytokine proliferative Recognition replicate replicated respiratory respiratory epithelia respiratory epithelial cell resulting RIG Stat1 tissue TLR TLR3 TLR7 TNF-α tracheal transcription factor Transcriptional upregulation triggered turn upper respiratory tract upregulation virus vomiting [DOI] 10.1128/mSphere.00820-21 PMC 바로가기 [Article Type] Research Article
Effect of Angiotensin-Converting-Enzyme Inhibitor and Angiotensin II Receptor Antagonist Treatment on ACE2 Expression and SARS-CoV-2 Replication in Primary Airway Epithelial CellsPharmacology Published on 2021-11-192022-10-31 Journal: Frontiers in Pharmacology [Category] COVID-19, [키워드] ACE2 ACE2 expression ACEi ACEIs AEC Air-liquid interface airway airway epithelial cell airway epithelium Angiotensin II angiotensin II (A II) receptor antagonists angiotensin-converting eitzyme inhibitors angiotensin-converting enzyme 2 antagonist antagonists ARB binding Biosafety Level 3 BSL-3 Captopril cardiovascular system Cell cells change changed children Clinical data convert Culture culture media cultures disease Effect epithelium Ex vivo expression generate IFN IFNB1 IFNL2 IL-1B IL-6 Infection infection with SARS-CoV-2 inhibitors interferon kidney losartan medication modify modulate MOI multiplicity of infection not significantly different poor prognosis Proinflammatory cytokine protective effect Protein qPCR quantitative PCR receptor reduce Replication responses risk risk of COVID-19 RNA SARS-CoV-2 SARS-CoV-2 replication SARS—CoV—2 significantly the SARS-CoV-2 treated Treatment type I USA-WA1/2020 viral spike protein were assessed were infected with COVID-19 [DOI] 10.3389/fphar.2021.765951 PMC 바로가기 [Article Type] Pharmacology
Immunohistochemistry for protein detection in PFA-fixed paraffin-embedded SARS-CoV-2-infected COPD airway epitheliumPFA 고정 파라핀 포매 SARS-CoV-2 감염 COPD 기도 상피에서 단백질 검출을 위한 면역조직화학Article Published on 2021-09-172022-09-11 Journal: STAR protocols [Category] SARS, 치료기술, 치료법, [키워드] Air-liquid interface airway airway epithelium antibody Cell chronic lung disease chronic obstructive COPD differentiation epithelial Health Sciences immunology in vitro model in vivo Lung disease Microbiology Microscopy NHBE paraffin-embedded Patient Protein protocol protocols provide SARS-CoV-2 SARS-COV-2 infection severe disease [DOI] 10.1016/j.xpro.2021.100663 PMC 바로가기 [Article Type] Article
Characterization of the SARS-CoV-2 Host Response in Primary Human Airway Epithelial Cells from Aged IndividualsArticle Published on 2021-08-122022-10-28 Journal: Viruses [Category] COVID-19, MERS, SARS, [키워드] addition age aging air–liquid interface air–liquid interface airway airway epithelium bronchial epithelial cell cell death changes in characterization characterized coronavirus 2 coronavirus disease COVID-19 Culture Donor epithelial exaggerated immune response global pandemic host response Human immune response in vitro study individual individuals Infection Inflammation innate immune response Innate immunity interferon response Older pathway performed Proinflammatory response provide respiratory Respiratory illness response risk factor SARS-CoV-2 SARS-COV-2 infection severe COVID-19 the SARS-CoV-2 [DOI] 10.3390/v13081603 PMC 바로가기 [Article Type] Article
Up-regulation of ACE2, the SARS-CoV-2 receptor, in asthmatics on maintenance inhaled corticosteroidsResearch Published on 2021-07-072022-10-28 Journal: Respiratory Research [Category] COVID-19, SARS, [키워드] ACE2 ACE2 expression Affect airway airway epithelium analyzed Asthma binding controls coronavirus demonstrated enzyme eosinophil expression female Gene Expression healthy control ICS IgE increased risk inhaled corticosteroid Large airway epithelium long-term treatment Maintenance inhaled corticosteroids male Microarrays no difference observation pandemic receptor Result SARS-CoV-2 SARS-COV-2 infection serum severe disease Sex subgroup analysis supplementary material the SARS-CoV-2 treated Treatment up-regulation virus [DOI] 10.1186/s12931-021-01782-0 PMC 바로가기 [Article Type] Research