Luminescent Assay for the Screening of SARS-CoV-2 M Pro InhibitorsSARS-CoV-2 M Pro 억제제의 스크리닝을 위한 발광 분석Article Published on 2022-08-032022-09-11 Journal: Chembiochem [Category] COVID19(2023년), SARS, 치료제, [키워드] antiviral drug can be used cleavage site Enzymes exhibit imaging agents in viral Infection inhibitor inhibitors Luminescence luminescence. M pro outbreak peptide Pro probe protease Replication reported SARS-CoV-2 Screening viral replication [DOI] 10.1002/cbic.202200190 PMC 바로가기 [Article Type] Article
A proteomics-MM/PBSA dual approach for the analysis of SARS-CoV-2 main protease substrate peptide specificitySARS-CoV-2 주요 프로테아제 기질 펩타이드 특이성 분석을 위한 proteomics-MM/PBSA 이중 접근법Article Published on 2022-08-012022-09-11 Journal: Peptides [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 치료기술, 치료제, [키워드] Amine amino acids Analysis approach Area binding energy carried cleavage site Combination COVID-19 determine diagnostic drug drug target Effects enzyme expected free energy Free energy calculations Glutamine identify indicated introduced lung M pro main protease Mechanics MM/PBSA Molecular mechanics pathogenicity peptide Perspective protease proteome proteomics SARS-CoV-2 SARS-CoV-2 main protease specificity structural homology substrate substrates surface surface area TAILS. terminal unique variant virus [DOI] 10.1016/j.peptides.2022.170814 PMC 바로가기 [Article Type] Article
A circular mRNA vaccine prototype producing VFLIP-X spike confers a broad neutralization of SARS-CoV-2 variants by mouse seraVFLIP-X 스파이크를 생성하는 원형 mRNA 백신 프로토타입은 마우스 혈청에 의한 SARS-CoV-2 변이체의 광범위한 중화를 제공합니다Article Published on 2022-08-012022-09-11 Journal: Antiviral Research [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, [키워드] addition Amino acid amino acid sequence amino acids Antibody titers B.1.1.529 B.1.617.2 balance BNT162b2 boost cellular immune responses cleavage site COVID-19 vaccine Critical D614G detectable Disulfide disulfide bond E484K Evolution of SARS-CoV-2 FIVE flexible glycosylation greater humoral Humoral and cellular immune responses immune responses immunization immunized induce K417N L452R linear linker mice mRNA mRNA vaccine mRNA vaccines mRNA-1273 N501Y neutralization Neutralizing activity Neutralizing antibody response Neutralizing antibody titer neutralizing antibody titers offer pre-fusion producing proline proline substitutions prolines reduced response S-2P SARS-CoV-2 SARS-CoV-2 vaccine SARS-CoV-2 variant SARS-CoV-2 variants SARS-CoV-2 virus sequence sera shown spike Spike protein spike trimer stability suggested T478K the spike protein transcript trimer trimeric Vaccine Vaccines variant variants variants of concern variants of interest VFLIP virus VOCs VOIs wildtype [DOI] 10.1016/j.antiviral.2022.105370 PMC 바로가기 [Article Type] Article
Comparative analysis of SARS-CoV-2 quasispecies in the upper and lower respiratory tract shows an ongoing evolution in the spike cleavage site상부 및 하부 호흡기에서 SARS-CoV-2 유사종의 비교 분석은 스파이크 절단 부위에서 진행 중인 진화를 보여줍니다Article Published on 2022-07-022022-09-11 Journal: Virus research [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Cell cleavage site Comparative analysis coronavirus deep deep sequencing Deleterious mutations evidenced Evolution explained genomic humans incidence INDEL indels intensive care intensive care unit locus Lower respiratory tract LRT Macrophage Minority variants Mutation Next-generation sequencing Non-synonymous mutation Patient phagocytosis Quasispecies quasispecy Result S gene SARS-CoV-2 selective severe acute respiratory syndrome Coronavirus specimens spike Spike protein spillover the Spike upper and lower respiratory tract upper respiratory tract URT variant variants viral quasispecy virus was performed [DOI] 10.1016/j.virusres.2022.198786 PMC 바로가기 [Article Type] Article
Ligand-based and structure-based studies to develop predictive models for SARS-CoV-2 main protease inhibitors through the 3d-qsar.com portal3d-qsar.com 포털을 통해 SARS-CoV-2 주요 프로테아제 억제제에 대한 예측 모델을 개발하기 위한 리간드 기반 및 구조 기반 연구Article Published on 2022-07-012022-09-11 Journal: Journal of computer-aided molecular design [Category] COVID19(2023년), SARS, 치료제, [키워드] 3-D QSAR Analysis applicability approach binding binding energy build calculation Candidates cleavage site co-crystallized Combination COMBINE Compound conservation coronavirus COVID-19 infected patients develop domain drug design Emergency use authorization enzyme EUA feature Final functional protein implicated in viral inhibitor inhibitors joint Ligand ligand-based drug design M pro maturation Metrics Mild-to-moderate Model molecular Nirmatrelvir outcome performed portal Predictive Predictive model promote protease Quantitative reached Replication reported resources robust robustness safety profile SARS-CoV-2 SARS-CoV-2 main protease statistical Structure-activity relationships Structure-based drug design tolerable training set Transcription Treatment U.S. FDA viral replication Virtual screening [DOI] 10.1007/s10822-022-00460-7 PMC 바로가기 [Article Type] Article
Novel cleavage sites identified in SARS-CoV-2 spike protein reveal mechanism for cathepsin L-facilitated viral infection and treatment strategies Research Published on 2022-06-062022-10-05 Journal: Cell Discovery [Category] 변종, 치료제, [키워드] activated acute respiratory syndrome approach blocked cathepsin Cell cleavage site conformations conserved coronavirus CTSL CTSL cleavage demonstrated domain Effect Ex vivo exhibited experiment host protease human donor Infection inhibitor Live virus lung tissue mechanism membrane fusion mice mutated novel omicron Prevent promoted Protein pseudovirus RBD reduced S protein SARS-CoV-2 SARS-CoV-2 S protein SARS-CoV-2 spike protein SARS-CoV-2 variant SARS-CoV-2 variants strain the S protein therapy Treatment treatment strategy Vaccine variant viral infection virus [DOI] 10.1038/s41421-022-00419-w [Article Type] Research
Insight towards the effect of the multi basic cleavage site of SARS-CoV-2 spike protein on cellular proteasesArticle Published on 2022-06-062022-11-01 Journal: Virus research [Category] COVID-19, COVID19(2023년), MERS, SARS, [키워드] acute respiratory syndrome Amino acid amino acid substitution Analysis analyzed Antiviral approach binding cathepsin B cell receptor cellular cellular protease cellular proteases cleavage site cleaving coronavirus coronavirus pathogenesis develop domain functional furin furin cleavage site generate Health help Host host cell protease host protease host range incorporation indicated Infection inhibitor insight Interaction involved maturation MD simulation MD simulations molecular molecular docking multi Mutation plasmin polybasic primary determinant protease Protein proteolytic activation Receptor binding S protein S1 and S2 SARS-CoV-2 SARS-CoV-2 spike protein shown spike Spike protein Spread subunits the S protein tissue tropism [DOI] 10.1016/j.virusres.2022.198845 PMC 바로가기 [Article Type] Article
Understanding the Driving Forces That Trigger Mutations in SARS-CoV-2: Mutational Energetics and the Role of Arginine Blockers in COVID-19 TherapySARS-CoV-2에서 돌연변이를 유발하는 원동력 이해: 돌연변이 에너지와 COVID-19 치료에서 아르기닌 차단제의 역할Article Published on 2022-05-112022-09-11 Journal: Viruses [Category] COVID19(2023년), SARS, 변종, [키워드] 3CLpro accelerated ACE2 ACE2 protein Alpha Amino acid amino acids angiotensin Angiotensin receptor blockers angiotensin type1 receptor Angiotensin-converting enzyme anionic antiviral drug arginine assist association Beta binding biphenyl bisartans blocker blockers candidate drug cleavage site cleavage sites complex complexes Computational approaches conformation conformational contribute COVID-19 COVID-19 therapy Critical Delta delta variant driving drug energetics ENhance equilibrium Evolution of SARS-CoV-2 force furin furin cleavage site Gamma human clinical trials human population hydrolysis hydropathy hydrophilic hydrophobic interaction in silico Infection Interaction interactions interface investigated Lambda molecular Molecular biology molecular dynamic simulations molecular dynamics mutant mutants mutate Mutation omicron Omicron variants pharmacophores polar Protein RBD RBD binding RBD-ACE2 receptor Receptor-binding domain referred to replacement Replication Research residue residues role S-protein salt salt bridges SARS-CoV-2 SARS-CoV-2 RBD SARS-CoV-2 variants SARS-CoV-2 virus sartans Spread suggested target targets tetrazole the RBD the receptor-binding domain the SARS-CoV-2 virus Therapies translation Treatment treatment of COVID-19 Trigger understanding variant variants viral infection virus wild-type virus π-π interaction [DOI] 10.3390/v14051029 PMC 바로가기 [Article Type] Article
Furin and TMPRSS2 Resistant Spike Induces Robust Humoral and Cellular Immunity Against SARS-CoV-2 Lethal Infection푸린 및 TMPRSS2 내성 스파이크는 SARS-CoV-2 치명적인 감염에 대한 강력한 체액 및 세포 면역을 유도합니다Article Published on 2022-05-022022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 ACE2 transgenic mice Against Amino acid antibody response against blocked C-terminal CD8 cleavage site clone clones COVID-19 vaccine effective Express furin furin cleavage furin cleavage site hamsters identify in some induce intranasal lethal Mutation mutations produced protective immunity Protein pseudotype Replication replication-competent. Resistant robust S antigen S protein S1/S2 cleavage S1/S2 cleavage site SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike SARS-CoV-2 spike protein SARS-CoV-2 variant single dose spike Spike protein T cell T cell response the SARS-CoV-2 TMPRSS2 transgenic unmet need vaccination Vaccine Vaccine design variant vesicular stomatitis vesicular stomatitis virus virus VSV was used [DOI] 10.3389/fimmu.2022.872047 PMC 바로가기 [Article Type] Article
The puzzling mutational landscape of the SARS-2-variant OmicronSARS-2 변종 오미크론의 수수께끼 같은 돌연변이 환경Article Published on 2022-05-012022-09-11 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] ACE-2 receptor affected antibody antibody susceptibility association cleavage site Complete coronavirus decrease deletions delta variant dissemination electrostatic Epitopes Evolution explain geometric human population immunological Infection initial initial assessment insertion less molecular mechanism molecular mechanisms mutations N-terminal domain neutralizing epitope neutralizing epitopes NTD omicron Omicron variant parameter Pathogenesis pathogenic phenotype predicted RBD Receptor-binding domain SARS Coronavirus SARS-CoV-2 variant Spike protein surface potential the receptor-binding domain Transmission Vaccine variant Virological virulence virus virus classification [DOI] 10.1002/jmv.27577 PMC 바로가기 [Article Type] Article