Development of an efficient one-step real-time reverse transcription polymerase chain reaction method for severe acute respiratory syndrome-coronavirus-2 detection중증 급성 호흡기 증후군-코로나 바이러스 -2 검출을위한 효율적인 1 단계 실시간 역전사 폴리머 라제 연쇄 반응 방법의 개발Research Article Published on 2021-06-042022-09-10 Journal: PLoS ONE [Category] MERS, SARS, 변종, 치료기술, [키워드] amplification amplified Contamination detect development diagnostic diagnostic testing DNA DNA polymerase enzyme Enzymes highest Internal control Murine leukemia virus polymerase chain polymerases quantification real-time RT-PCR respiratory reverse transcriptase reverse transcriptases reverse transcription RNA RNase P SARS-CoV-2 Sensitivity and specificity Severe acute respiratory syndrome standard curve Thermococcus Thermus transcriptase UNG uracil variant was obtained wild-type [DOI] 10.1371/journal.pone.0252789 PMC 바로가기 [Article Type] Research Article
Quick and Easy Assembly of a One-Step qRT-PCR Kit for COVID-19 Diagnostics Using In-House EnzymesArticle Published on 2021-03-152022-10-27 Journal: ACS Omega [Category] COVID-19, [키워드] applied approach assembly clinical sample component condition COVID-19 detect diagnosis of COVID-19 diagnostics DNA polymerase enzyme Invitrogen Laboratory Murine leukemia virus Platinum polymerase chain qRT-PCR Quantitative Rapid reliability response RNA robust RT-qPCR SARS-CoV-2 shown TaqPath team Thermus transcriptase viral titer [DOI] 10.1021/acsomega.0c05635 PMC 바로가기 [Article Type] Article
Direct detection of SARS-CoV-2 using non-commercial RT-LAMP reagents on heat-inactivated samples열 비활성화 샘플에서 비상업용 RT-LAMP 시약을 사용한 SARS-CoV-2의 직접 검출Article Published on 2021-01-192022-09-01 Journal: Scientific Reports [Category] 진단, 치료기술, [키워드] applicability approach compatibility condition contribute COVID-19 diagnostics Direct DNA polymerase Enzymes expansion facilitate heat-inactivated house nasopharyngeal sample Nasopharyngeal samples optimization Patient patients PCR-based techniques produced reagent required reverse transcriptase reverse transcriptases RNA RT-LAMP SARS-CoV-2 SARS-CoV-2 testing shown Spread the disease viral infection [DOI] 10.1038/s41598-020-80352-8 PMC 바로가기 [Article Type] Article
One-Enzyme Reverse Transcription qPCR Using Taq DNA PolymeraseTaq DNA 중합효소를 사용한 단일 효소 역전사 qPCRArticle Published on 2020-12-152022-09-11 Journal: Biochemistry [Category] SARS, 치료기술, [키워드] buffer CDC detect DNA DNA polymerase enzyme genomic RNA input Murine leukemia virus past PCR promote qPCR qPCR assays reverse transcriptase RT-qPCR RT-qPCR assay RT-qPCRs salt SARS-CoV-2 N1 shown Taq DNA polymerase Taq polymerase TaqMan transcriptase utility Viral viral genomic RNA [DOI] 10.1021/acs.biochem.0c00778 PMC 바로가기 [Article Type] Article
A blueprint for academic laboratories to produce SARS-CoV-2 quantitative RT-PCR test kitsArticle Published on 2020-11-132023-07-02 Journal: The Journal of Biological Chemistry [Category] SARS, [키워드] coronavirus DNA polymerase Formulation Infectious disease Polymerase chain reaction (PCR) Protein purification reverse transcriptase reverse transcription ribonuclease inhibitor RNA RT-qPCR SARS-CoV-2 virus. [DOI] 10.1074/jbc.RA120.015434 PMC 바로가기
Detection of SARS-CoV-2 from raw patient samples by coupled high temperature reverse transcription and amplificationResearch Article Published on 2020-11-022022-10-28 Journal: PLoS ONE [Category] COVID-19, MERS, SARS, [키워드] amplification approach consequence detect detection DNA polymerase elevated temperature equipment FIVE gold standard handling high temperature infected individual Patient PCR amplification protocol purification purify RNA reagents reduce reverse transcription RNA RNA virus RT-PCR SARS-CoV-2 Surveillance target gene viral genome virus [DOI] 10.1371/journal.pone.0241740 PMC 바로가기 [Article Type] Research Article
Sofosbuvir terminated RNA is more resistant to SARS-CoV-2 proofreader than RNA terminated by RemdesivirArticle Published on 2020-10-062022-10-04 Journal: Scientific Reports [Category] MERS, SARS, 치료제, [키워드] activity Antiviral Biochemistry Brain Chemical biology clinical trial clinical trials Combination Coronavirus replication COVID-19 daclatasvir demonstrated DNA polymerase drug Drug discovery effective EPCLUSA exonuclease genetics Hepatitis hepatitis C virus inhibit SARS-CoV-2 inhibitor initiated investigator lung maintain molecular molecular basis offer Other pandemic polymerase Proofreading RdRP RdRps Replication responsible resulting RNA RNA-dependent RNA polymerase SARS-CoV SARS-CoV-2 sofosbuvir Structure the SARS-CoV-2 therapeutic viral genome [DOI] 10.1038/s41598-020-73641-9 PMC 바로가기 [Article Type] Article
Comparative Antiviral Activity of Remdesivir and Anti-HIV Nucleoside Analogs against Human Coronavirus 229E (HCoV-229E)Brief Report Published on 2020-05-172022-10-30 Journal: Molecules [Category] COVID-19, MERS, SARS, [키워드] 229E activity Antiviral antiviral activity can be used cells clinical trial Comparative Compound compounds concentrations conjugate Conjugates COVID-19 cytotoxicity determine DNA polymerase EFdA exhibited fibroblast HCoV-229E Human coronavirus human coronavirus 229E inactive inhibitor interfere lung cell normal NRTIs nucleoside nucleotide prodrug other coronaviruses parent Remdesivir RNA polymerase RNA-dependent RNA polymerase SARS-CoV-2 tested Treatment were used with HIV [DOI] 10.3390/molecules25102343 PMC 바로가기 [Article Type] Brief Report
Differences in the Likelihood of Acyclovir Resistance-Associated Mutations in the Thymidine Kinase Genes of Herpes Simplex Virus 1 and Varicella-Zoster VirusArticle Published on 2019-03-112023-05-31 Journal: Antimicrobial Agents and Chemotherapy [Category] 두창, 수두, 홍역, [키워드] acyclovir acyclovir resistant DNA polymerase Herpes Simplex Virus 1 thymidine kinase Varicella-Zoster Virus [DOI] 10.1128/AAC.00017-19 PMC 바로가기
Genetic polymorphism of thymidine kinase (TK) and DNA polymerase (pol) of clinical varicella-zoster virus (VZV) isolates collected over three decadesArticle Published on 2017-10-012023-06-01 Journal: Journal of Clinical Virology [Category] 두창, 수두, 홍역, [키워드] DNA polymerase Genotypic resistance testing Natural gene polymorphism thymidine kinase Varicella-zoster virus. [DOI] 10.1016/j.jcv.2017.08.011 PMC 바로가기