DNA Vaccines Expressing the Envelope and Membrane Proteins Provide Partial Protection Against SARS-CoV-2 in Mice엔벨로프 및 막 단백질을 발현하는 DNA 백신은 생쥐에서 SARS-CoV-2에 대한 부분적 보호를 제공합니다Article Published on 2022-02-242022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Against caused cellular Cellular immune response cellular response conserved coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 cross-protective DNA DNA vaccine dose effective Electroporation elicited envelope envelope protein Epitopes humoral Humoral immunity Humoral response immune responses immunisation International Intramuscular injection lung tissue membrane membrane protein membrane protein. mice pandemic Partial protection Protein provided public health emergency respiratory robust SARS-CoV SARS-CoV-2 severe acute respiratory syndrome Coronavirus Significant structural protein structural proteins T-cell epitope target titre Vaccination strategies Vaccination strategy Vaccine vaccine candidate vaccine candidates Vaccines virus [DOI] 10.3389/fimmu.2022.827605 PMC 바로가기 [Article Type] Article
Variable levels of spike and ORF1ab RNA in post-mortem lung samples of SARS-CoV-2-positive subjects: comparison between ISH and RT-PCRSARS-CoV-2 양성 대상자의 사후 폐 샘플에서 다양한 수준의 스파이크 및 ORF1ab RNA: ISH와 RT-PCR 간의 비교Original Article Published on 2022-02-012022-09-10 Journal: Virchows Archiv [Category] COVID19(2023년), MERS, SARS, 치료기술, 치료법, 치료제, [키워드] amplification amplification cycle amplification cycles analyzed cellular Chain Reaction correlation Deceased demonstrated detect E gene electron microscopy envelope FFPE formalin formalin-fixed paraffin-embedded Frame gene amplification IHC immunohistochemistry In situ hybridization In situ hybridization (ISH) Microscopy morphology N gene nucleocapsid Open reading frame Open reading frame (ORF1ab) optimization ORF1 ORF1ab ORF1ab gene paraffin polymerase chain polymerase chain reaction positive Post-mortem post-mortem lung Post-mortem lung samples reverse transcription reverse transcription polymerase chain reaction Reverse transcription polymerase chain reaction (RT-PCR) RNA RT-PCR S gene SARS-CoV-2 sensitivity sequence Spike(S) staining subjects supplementary material the SARS-CoV-2 tissue variable Viral Viral RNA virus virus detection [DOI] 10.1007/s00428-021-03262-8 PMC 바로가기 [Article Type] Original Article
Designing of peptide aptamer targeting the receptor-binding domain of spike protein of SARS-CoV-2: an in silico studySARS-CoV-2의 스파이크 단백질 수용체 결합 도메인을 표적으로 하는 펩타이드 앱타머 설계: in silico 연구Article Published on 2022-02-012022-09-11 Journal: Molecular diversity [Category] COVID19(2023년), SARS, 치료법, 치료제, [키워드] ACE2 albumin antibodies approach aptamer ATP Bacterial bind CD4 complex coronavirus docked domain ENCODE envelope Genome high affinity host cell in silico membrane metal ions molecular molecular docking molecular dynamic simulation monoclonal antibodies non-allergenic non-hemolytic nucleocapsid peptide Peptide aptamer predicted Protein Proteins Receptor-binding domain SARS-CoV-2 scaffold protein serum albumin specificity spike Spike protein spike protein. spike receptor-binding domain stability target target protein tertiary structure the receptor-binding domain theophylline Therapeutic treatment thioredoxin thrombin virus [DOI] 10.1007/s11030-020-10171-6 PMC 바로가기 [Article Type] Article
Population wide testing pooling strategy for SARS-CoV-2 detection using salivaResearch Article Published on 2022-01-282022-10-28 Journal: PLoS ONE [Category] COVID-19, SARS, [키워드] analyzed applied average COVID-19 cycle cycle threshold detect diagnosis of COVID-19 diagnostic effective envelope Genes Healthcare professional identify individuals investigated Median viral load nasopharyngeal sample no significant difference Outbreaks Pain patients Population populations positive predicted reaction reading RT-PCR Saliva saliva sample SARS-CoV-2 SARS-CoV-2 detection SARS-COV-2 infection SARS-CoV-2 pandemic sensitivity significant difference specificity suggested symptomatic and asymptomatic [DOI] 10.1371/journal.pone.0263033 PMC 바로가기 [Article Type] Research Article
Omicron SARS-CoV-2 variant: Unique features and their impact on pre-existing antibodiesOmicron SARS-CoV-2 변종: 고유한 기능 및 기존 항체에 미치는 영향Article Published on 2022-01-012022-09-11 Journal: Journal of autoimmunity [Category] SARS, 변종, 진단, 치료법, [키워드] Affect analysis Analysis antibodies antibody Antibody binding B.1.1.529 binding affinities concern coronavirus COVID-19 distribution envelope feature Gamma information membrane Mutation nucleocapsid omicron Omicron variant P.1 Phylogenetic analysis Prevalence Protein reported respiratory Respiratory Coronavirus S protein SARS-CoV-2 severe acute respiratory coronavirus structural protein structural proteins the binding affinity the S protein variant variants variants. virus VoC [DOI] 10.1016/j.jaut.2021.102779 PMC 바로가기 [Article Type] Article
Prevalence of a Single-Nucleotide Variant of SARS-CoV-2 in Korea and Its Impact on the Diagnostic Sensitivity of the Xpert Xpress SARS-CoV-2 AssaySARS-CoV-2의 단일 뉴클레오티드 변이체의 국내 유병률과 Xpert Xpress SARS-CoV-2 분석의 진단 민감도에 미치는 영향Article Published on 2022-01-012022-09-11 Journal: Annals of laboratory medicine [Category] SARS, 변종, 진단, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 affected Cepheid clinical laboratories clinical laboratory coronavirus coronavirus disease Coronavirus disease 2019 Coronavirus disease 2019 (COVID-19) Diagnosis diagnostic diagnostic sensitivity diagnostics dominant Effect envelope Genes Impact impair Korea Lineage molecular molecular diagnostics N gene N genes nucleocapsid nucleotide pathogen performance prevalent respiratory Sanger sequencing SARS-CoV-2 SARS-CoV-2 genome sensitivity severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) significant difference significant differences Single nucleotide variant single-nucleotide SNV the SARS-CoV-2 genome threshold USA variant virus Xpert Xpress SARS-CoV-2 assay. [DOI] 10.3343/alm.2022.42.1.96 PMC 바로가기 [Article Type] Article
Clinical Evaluation of the Rapid STANDARD Q COVID-19 Ag Test for the Screening of Severe Acute Respiratory Syndrome Coronavirus 2중증급성호흡기증후군 코로나바이러스 2 선별을 위한 Rapid STANDARD Q COVID-19 Ag 테스트의 임상 평가Article Published on 2022-01-012022-09-11 Journal: Annals of laboratory medicine [Category] SARS, 치료기술, 치료법, 치료제, [키워드] 2019-nCoV acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Allplex 2019-nCoV Assay alternative antibodies Antigen case-control study clinical collected compensated coronavirus coronavirus 2 COVID-19 cycle cycle threshold detect envelope evaluated FIVE Genes gold immunochromatography Inc. nucleocapsid nucleocapsid protein Patient performance Rapid respiratory respiratory syndrome coronavirus 2 retrospective reverse transcription RNA-dependent RNA polymerase rRT-PCR test rRT-PCR tests SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 gene SARS-CoV-2 genes Screening SD Biosensor sensitivity Sensitivity and specificity Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Severe acute respiratory syndrome coronavirus 2. significantly lower Standard STANDARD Q COVID-19 Ag test symptom onset Test threshold Viral RNA [DOI] 10.3343/alm.2022.42.1.100 PMC 바로가기 [Article Type] Article
Modulation of the NLRP3 inflammasome by Sars-CoV-2 Envelope proteinArticle Published on 2021-12-242022-10-04 Journal: Scientific Reports [Category] SARS, 변종, [키워드] Activation approach approach Coronaviruses COVID-19 cultured macrophage Cytokine storm drug E protein early stage Effect envelope Evidence examined expression Host human macrophages IL-18 IL-1β immunology Infection Inflammasome inflammasome activation Inflammatory response initial LPS lung Macrophage Macrophage infiltration mechanism mice Microbiology murine NLRP3 inflammasome Pathogenesis Patient priming Protein pulmonary inflammation reduced response SARS-CoV-2 disease Stage suggested suppress suppressed targeting therapy transfected with treated Treatment Vaccine Viral RNA virulence [DOI] 10.1038/s41598-021-04133-7 PMC 바로가기 [Article Type] Article
Functional Trimeric SARS-CoV-2 Envelope Protein Expressed in Stable CHO CellsBioengineering and Biotechnology Published on 2021-12-172022-10-31 Journal: Frontiers in Bioengineering and Biotechnology [Category] COVID-19, [키워드] ACE2 acute respiratory syndrome Cell cellular CHO cell coronavirus COVID-19 pandemic COVID–19 CR3022 described docking domain envelope envelope protein expressing expression Extracellular infection mechanism infection with SARS-CoV-2 integrated bioprocessing Interaction mandatory membrane-bound mutant pandemic parameter Perfusion Protein protocol purification Recombinant spike protein responsible robust S-protein SARS CoV-2 SARS-CoV-2 similarity spike (S) glycoprotein Spike protein STABLE surface protein surface proteins the receptor-binding domain the spike protein therapeutic drug trimeric variant viral envelope proteins virus β-coronavirus [DOI] 10.3389/fbioe.2021.779359 PMC 바로가기 [Article Type] Bioengineering and Biotechnology
Nucleocapsid (N) Gene Mutations of SARS-CoV-2 Can Affect Real-Time RT-PCR Diagnostic and Impact False-Negative ResultsSARS-COV-2의 NucleoCapsid (N) 유전자 돌연변이는 실시간 RT-PCR 진단에 영향을 줄 수 있으며 거짓 음성 결과에 영향을 줄 수 있습니다.Communication Published on 2021-12-102022-09-01 Journal: Viruses [Category] SARS, 변종, 진단, 치료기술, 치료법, [키워드] N gene Affect affecting alteration amino acids annealing Brazil Can caused chain Chain Reaction characterization circulation Continuous continuous monitoring COVID-19 COVID-19 pandemic Deletion diagnosis of COVID-19 diagnostic diagnostic test disorder Effectiveness envelope false false-negative false-negative results frameshift frameshift mutation GeneFinder genomic gold gold standard identify Impact intrinsic lead maintain Mutation N gene network nucleotide nucleotides pathogenicity phenotypic polymerase chain reaction polymerase chain reaction) positive sample primer Primers probe probes protein level RdRP reaction real-time RT-PCR Result reverse transcription polymerase chain reaction RNA polymerase RNA-dependent RNA polymerase RT-PCR SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 genomes SARS-CoV-2 variants SARS-CoV-2 virus sequenced sequenced sample Substitution Surveillance the SARS-CoV-2 virus Transmissibility Vaccine virus [DOI] 10.3390/v13122474 PMC 바로가기 [Article Type] Communication