Extension of Collagen Deposition in COVID-19 Post Mortem Lung Samples and Computed Tomography Analysis FindingsCOVID-19 사후 폐 샘플 및 컴퓨터 단층 촬영 분석 결과에서 콜라겐 침착의 확장Article Published on 2021-07-132022-09-10 Journal: International Journal of Molecular Sciences [Category] SARS, 진단, 치료기술, [키워드] 95% CI 95% confidence interval aeration Analysis analyzed collagen Computed tomography correlation correlations COVID-19 COVID-19 patient COVID-19 pneumonia Critically ill critically ill COVID-19 patients CT finding Deceased deposition detect extension fibrosis finding fixed formalin fraction histopathological Invasive mechanical ventilation investigated Lung CT Lung fibrosis median moderate paraffin patients with COVID-19 Pneumonia Postmortem Quantitative Radiographic Sample sensitivity Sirius specificity transbronchial [DOI] 10.3390/ijms22147498 PMC 바로가기 [Article Type] Article
SARS-CoV-2 RNA screening in routine pathology specimens일상적인 병리 표본에서 SARS-CoV-2 RNA 스크리닝Article Published on 2021-07-012022-09-11 Journal: Microbial Biotechnology [Category] SARS, 진단, 치료기술, [키워드] approach biopsies bowel Cohort Colon confirmed COVID-19 patients COVID-19 COVID-19 case COVID-19 patients diagnostic Effects FFPE formalin formalin-fixed and paraffin-embedded IMPROVE lung lung biopsy Mild multiple organ multiple organs no symptoms organ pandemic Pandemics pathology Patient patients pleural effusion protocol retrospective RT-PCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 RNA detection selected Seven severe cases specimen Surveillance Swab tested the SARS-CoV-2 tissue [DOI] 10.1111/1751-7915.13828 PMC 바로가기 [Article Type] Article
CVnCoV and CV2CoV protect human ACE2 transgenic mice from ancestral B BavPat1 and emerging B.1.351 SARS-CoV-2CVnCoV 및 CV2CoV는 조상 B BavPat1 및 신흥 B.1.351 SARS-CoV-2로부터 인간 ACE2 형질전환 마우스를 보호합니다Article Published on 2021-06-302022-09-10 Journal: Nature Communications [Category] MERS, SARS, 변종, 진단, 치료기술, [키워드] antibody Antibody titers B.1.351 B.1.351 variant BavPat1 Brain caused clinical development Complete controls CVnCoV detectable develop disease Efficacy elevated formalin groups hACE2 host immunity human ACE2 immunized K18-hACE2 lung mice Mortality mRNA mRNA vaccine mRNA vaccines mutant Mutation neutralization titer neutralization titers Neutralizing antibody titer PROTECT raise reduced SARS-CoV-2 SARS-CoV-2 pandemic SARS-CoV-2 variant SARS-CoV-2 variants significantly Spike protein swabs the spike protein transgenic mice Transgenic mouse Vaccine vaccine efficacy Vaccines variants variants of concern Viral viral strain VoC was tested were used [DOI] 10.1038/s41467-021-24339-7 PMC 바로가기 [Article Type] Article
Immunogenicity of Adjuvanted Psoralen-Inactivated SARS-CoV-2 Vaccines and SARS-CoV-2 Spike Protein DNA Vaccines in BALB/c MiceArticle Published on 2021-05-192022-10-28 Journal: Pathogens [Category] COVID-19, [키워드] 4′-aminomethyl-4,5′,8-trimethylpsoralen (AMT) adjuvants Administered Advax-2 anti-SARS-CoV-2-neutralizing antibodies BALB/c boost candidate conformational epitopes of surface antigens COVID-19 cytokine DNA DNA vaccine dose-dependent doses effective ELISPOT response evaluated expressing formalin full-length immunogenicity inactivation indicate mice Neutralizing antibody response Prime-boost produced promote PROTECT psoralen-inactivated SARS-CoV-2 vaccine regimen Safe SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 vaccine spike Spike proteins Stage Th2-type Vaccine vaccine candidate [DOI] 10.3390/pathogens10050626 PMC 바로가기 [Article Type] Article
Inactivation and Elimination of SARS-CoV-2 in Biosamples Using Simple Fixatives and UltrafiltrationProtocol Published on 2021-02-272022-10-30 Journal: Methods and Protocols [Category] COVID-19, [키워드] accelerated affected Analysis biocontainment biological sample BSL-3 BSL2 cause Cell Cell culture Complete conducted COVID-19 cut-off downstream analysis effective Effectiveness eliminating Elimination Ethanol Ex vivo fixative formalin handling Health host cell in vitro in vivo model inactivated inactivation individual Infection infection risk kDa Laboratory limit lung tissue lysates media membrane membrane filter molecular weight neutral buffered formalin pandemic paraformaldehyde pathogenesis of SARS-CoV-2 pathogenicity pathological analysis plaque forming units reduce Research respiratory Safe SARS-CoV-2 SARS-CoV-2 infected cell significantly simple standard procedure subsequent tissue Treatment Ultrafiltration undetectable viral inoculum virus [DOI] 10.3390/mps4010018 PMC 바로가기 [Article Type] Protocol
[Histopathological features due to the SARS-CoV-2][SARS-CoV-2로 인한 조직병리학적 특징]Review Published on 2021-02-012022-09-11 Journal: Annales de pathologie [Category] MERS, SARS, 진단, 치료기술, [키워드] Analysis Ancillary tools approaches Autopsie Autopsy autopsy studies Brain cells cellular co-morbidity concerning consequence COVID-19 COVID-19 positive patient COVID-19 positive patients English epidemiological Étude Études ancillaires. Factors feature fixed formalin French gastrointestinal tract heart histological immunohistochemistry IMPROVE In situ hybridization Infection Infectious disease kidney knowledge leads lesion lesions liver lung Microscopy Molecular biology observation organs paraffin Pathologie pathology pathophysiological mechanism pathophysiological mechanisms pathophysiology Patient Physiopathologie respiratory respiratory tract SARS-CoV-2 skin the SARS-CoV-2 therapeutic strategy tissue tissues virus [DOI] 10.1016/j.annpat.2020.12.009 PMC 바로가기 [Article Type] Review
Inactivation of Material from SARS-CoV-2-Infected Primary Airway Epithelial Cell CulturesLetter Published on 2021-01-072022-10-30 Journal: Methods and Protocols [Category] COVID-19, [키워드] acute respiratory syndrome AEC airway airway epithelium BSL-3 BSL-3 laboratory CDC Cell Cell culture center conducted Control coronavirus COVID-19 Culture downstream Effectiveness epithelial epithelial cell evaluated experiment facilitate Factor fixation fixed formalin Guidance heat Heating immunohistochemistry inactivated inactivation Infection initial innate immune response Isolation Laboratory level 3 live SARS-CoV-2 material prevention primary human airway Protein protocol protocols RNA Safe SARS-CoV-2 SDS sodium supernatant tested transfer Treatment Triton X100 ultraviolet UV light Vero E6 Vero E6 cell viral entry virus [DOI] 10.3390/mps4010007 PMC 바로가기 [Article Type] Letter
Inflammasome formation in the lungs of patients with fatal COVID-19치명적인 COVID-19 환자의 폐에서 Inflammasome 형성Article Published on 2021-01-012022-09-12 Journal: Inflammation research : official journal of the Eu [Category] 진단, [키워드] acute respiratory distress aggregate ARDS ASC Cardiopulmonary caspase-1 cleaved collected conducted control subject coronavirus SARS-CoV COVID-19 cytokine Cytokine release syndrome died disease domain embedded in paraffin Evidence expression fatal COVID-19 fixed formalin Hospitalized identify IL-1 in vitro Inflammasome Italy leukocytes lung Lung injury Macrophage NLRP3 NLRP3 inflammasome on mechanical ventilation Patient Pneumonia potential molecular Protein protein 3 Pulmonary infection recruitment Repository Sample SARS-CoV-2 significantly higher staining subject syndrome Trieste Trigger university viral infection [DOI] 10.1007/s00011-020-01413-2 PMC 바로가기 [Article Type] Article
Development of immunohistochemistry and in situ hybridisation for the detection of SARS-CoV and SARS-CoV-2 in formalin-fixed paraffin-embedded specimens포르말린 고정 파라핀 포매 표본에서 SARS-CoV 및 SARS-CoV-2 검출을 위한 면역조직화학 및 in situ hybridization 개발Article Published on 2020-12-142022-09-01 Journal: Scientific Reports [Category] MERS, SARS, 진단, 치료기술, 치료법, [키워드] addition against SARS-CoV animal animal models antibody Antigen antigen retrieval method applied causative agent caused coronavirus coronavirus pathogenesis COVID-19 demonstrated detect determine development dissemination evaluated expressing facilitate FFPE flexibility formalin formalin-fixed paraffin-embedded IHC immunohistochemistry infected cell infected cells Live virus MERS Microscopy non-infected cell non-infected cells nucleoprotein paraffin positive control probe protocol public health Research RNA SARS-CoV SARS-CoV spike protein SARS-CoV-2 SARS-CoV-2 viral specimen spike gene Spike protein tested therapeutic Therapeutics tissues utility Vaccine Viral virus virus detection [DOI] 10.1038/s41598-020-78949-0 PMC 바로가기 [Article Type] Article
Evaluation of different inactivation methods for high and low pathogenic avian influenza viruses in egg-fluids for antigen preparationArticle Published on 2015-09-152023-06-02 Journal: Journal of virological methods [Category] 조류인플루엔자, [키워드] Avian Influenza BPL ether formalin inactivation [DOI] 10.1016/j.jviromet.2015.05.004