Disruption of the Golgi Apparatus and Contribution of the Endoplasmic Reticulum to the SARS-CoV-2 Replication ComplexArticle Published on 2021-09-092022-10-28 Journal: Viruses [Category] COVID-19, SARS, [키워드] addition apparatus appear caused Cell Biology cellular change Complete complex Coronavirus replication Critical Disruption dsRNA electron microscopy Endoplasmic reticulum ER eukaryotic cells Evidence expressed FIVE Golgi Golgi apparatus host membrane immunolabeling implicated infected cell inhibitory marker mechanism membrane Microscopy Nsp3 nsp4 organelle Post-infection Protein Proteins Replication responsible Reticulum SARS-CoV-2 SARS-CoV-2 protein shown the SARS-CoV-2 variety Viral proteins viral replication viral RNA synthesis were used [DOI] 10.3390/v13091798 PMC 바로가기 [Article Type] Article
Sequences in the cytoplasmic tail of SARS-CoV-2 Spike facilitate expression at the cell surface and syncytia formationSARS-CoV-2 Spike의 세포질 꼬리에 있는 서열은 세포 표면에서의 발현 및 합포체 형성을 촉진합니다Article Published on 2021-09-092022-09-10 Journal: Nature Communications [Category] SARS, 변종, 유전자 메커니즘, [키워드] accumulate ACE2 Actin Adenovirus autophagy bind binding C-terminal Cell cell surface cellular COPI COPII cytoplasmic tail direct fusion domain Endoplasmic reticulum exit expressing expression facilitate Factor full-length S fusion Golgi histidine host cells induce Interaction lifecycle mass spectrometry motif mRNA promote Protein proteomic residue RNA vaccines SARS-CoV-2 SARS-COV-2 infection sequence sequences spike Spike protein Syncytia syncytia formation tail the cell the SARS-CoV-2 transmembrane domain understanding Vaccine vectors Vesicle Viral Virus-host interactions [DOI] 10.1038/s41467-021-25589-1 PMC 바로가기 [Article Type] Article
Early prediction of COVID‐19 severity using extracellular vesicle COPB2세포외소포 COPB2를 이용한 코로나19 중증도 조기예측Research Article Published on 2021-06-022022-09-10 Journal: Journal of Extracellular Vesicles [Category] MERS, SARS, 바이오마커, [키워드] 95% CI Acute respiratory failure Admission analyzed Antiviral antiviral response asymptomatic infection AUC best Biomarker clinical decision clinical manifestation clinical manifestations Cohort collected complex component COPB2 correlation correlations Corticosteroids COVID‐19 COVID‐19 patient COVID‐19 symptom COVID‐19 symptoms COVID‐19 death determinant Evidence EVs exhibited expression exRNAs Extracellular extracellular RNA extracellular RNAs extracellular vesicle Extracellular vesicles Golgi group healthy control healthy controls highlight Hypothesis indicate involved liver Liver damage marker Mild Patient Predictive Predictive value predictor profile profiles Protein Proteins remained Respiratory failure SARS‐CoV‐2 serum sample serum samples severity significantly higher Stratification subunit Symptomatic treatment therapy three group three groups Variability Vesicle [DOI] 10.1002/jev2.12092 PMC 바로가기 [Article Type] Research Article
Assembly and Cellular Exit of Coronaviruses: Hijacking an Unconventional Secretory Pathway from the Pre-Golgi Intermediate Compartment via the Golgi Ribbon to the Extracellular SpaceReview Published on 2021-02-262022-10-31 Journal: Cells [Category] COVID-19, MERS, [키워드] absence aggregate assembly bypasse carrier Cell compartment connection Coronavirus (CoV) CoV CoVs defined described element endocytic Endoplasmic reticulum endosome ER-Golgi intermediate compartment (IC or ERGIC) event exit extracellular space functional Golgi Golgi bypass Golgi ribbon GTPase indicate initial intermediate introduced Lipoproteins megavesicles pathway progeny promote Protein rab1 rab11 recycling endosome (RE) remained ribbon secretory Space Transport unconventional secretion vesicular tubular cluster (VTC) virus virus assembly virus egress viruses zone [DOI] 10.3390/cells10030503 PMC 바로가기 [Article Type] Review
On Drug-Membrane Permeability of Antivirals for SARS-CoV-2SARS-CoV-2에 대한 항바이러스제의 약물-막 투과성Article Published on 2021-02-112022-09-11 Journal: The journal of physical chemistry letters [Category] 치료제, [키워드] approach Behavior Bioavailability cellular organelles Characteristics Combination composed Compound contribute coronavirus determine drug drug design drugs effective drug effective drugs Efficacy endoplasmic reticulum membranes FIVE flexible Golgi highlight identify Interaction mammalian membrane membrane permeability membranes mitochondrial molecular dynamics Molecular dynamics simulation molecules negative correlation parameter permeability permeation plasma predict Proteins required SARS-CoV-2 Spike protein statistical analysis targets transmembrane variety Viral viral envelope [DOI] 10.1021/acs.jpclett.0c02397 PMC 바로가기 [Article Type] Article
Might proton pump or sodium‐hydrogen exchanger inhibitors be of value to ameliorate SARs‐CoV‐2 pathophysiology?Review Article Published on 2020-12-252022-10-28 Journal: Physiological Reports [Category] COVID-19, [키워드] administration animal model approach benefit biogenesis bronchitis candidate cathepsin Cell cell entry cleavage complex compounds contributed CoV COVID‐19 COVID‐19 Cytokine release syndrome disease pathogenesis Disease progression disrupt disrupting drug Efficacy endocytic endosomal complexes required for transport endosomal pH entry pathway envelope exacerbate Exosome Exosomes extracellular vesicle Extracellular vesicles glycosylation Golgi Infection inhibit inhibiting inhibitor involved mechanism membrane mice Microvesicles multivesicular body occur Pathogenesis plasma membrane PPI promote Pulmonary edema pulmonary epithelial cell reported serine protease TMPRSS2 Spike protein suggested therapeutic Transport Vesicle viral endocytosis viroporin viruses [DOI] 10.14814/phy2.14649 PMC 바로가기 [Article Type] Review Article
Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies통합 이미징은 SARS-CoV-2에 의해 유도된 세포 내 형태의 재구성을 보여줍니다Article Published on 2020-12-092022-09-12 Journal: Cell Host & Microbe [Category] MERS, SARS, 진단, [키워드] alteration Analysis biogenesis Cell cell death Cluster coronavirus cytokine response Cytopathic effect cytoskeleton dataset determine electron tomography elements Endoplasmic reticulum epithelial cells FIB-SEM Golgi Golgi apparatus human lung imaging Infection intermediate filaments live cell imaging membrane membrane remodeling Microscopy mitochondrial Morphological organelle peroxisome peroxisomes recruitment Repository reveal SARS-CoV-2 SARS-CoV-2 replication SARS-CoV-2-infected cell subcellular suppresse thought Vesicle viral replication viral replication organelles. [DOI] 10.1016/j.chom.2020.11.003 PMC 바로가기 [Article Type] Article
The SARS-CoV-2 envelope and membrane proteins modulate maturation and retention of the spike protein, allowing assembly of virus-like particlesResearch Article Published on 2020-12-032022-10-30 Journal: The Journal of Biological Chemistry [Category] COVID-19, SARS, [키워드] acute respiratory syndrome biochemical C-terminal Cell composed coronavirus COVID-19 COVID-19 pandemic Critical cytoplasmic tail DMEM, Dulbecco’s modified minimal essential medium E, Envelope Endoplasmic reticulum ERGIC ERGIC, endoplasmic reticulum (ER)–Golgi intermediate compartment glycoprotein Golgi highlight IBV, infectious bronchitis virus IF, immunofluorescence induce infected cell Infectious disease Interaction Like M protein M, membrane maturation membrane membrane protein MERS-CoV, Middle East Respiratory Virus modulate modulating motif N-glycosylation N, nucleoprotein other coronaviruses Particle Particles Prevent promote Protein Proteins purified regulate Regulation required S protein S, spike SARS-CoV SARS-CoV-2 SARS-CoV-2 envelope SARS-CoV-2 viral particles SARS-CoV-2-infected cells SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 secretion secretory pathway structural protein syncytia formation the spike protein transfected cells Viral particles Viral protein virus assembly Virus-like particle VLPs, Virus-like particles vRNP, viral ribonucleoprotein β-coronavirus [DOI] 10.1074/jbc.RA120.016175 PMC 바로가기 [Article Type] Research Article
SARS-CoV-2 viral budding and entry can be modeled using BSL-2 level virus-like particlesResearch Article Published on 2020-11-272022-10-30 Journal: The Journal of Biological Chemistry [Category] COVID-19, SARS, [키워드] ACE2, angiotensin-converting enzyme 2 acute respiratory syndrome BCA, bicinchoninic acid assay BSL-2 BSL-3 BSL-3 facilities circumvent competent conditions coronavirus DAB, 3,3′-Diaminobenzidine DMEM, Dulbecco’s modified Eagle’s medium EEA1, early endosome antigen 1 effort electron microscopy (EM) ERGIC, endoplasmic reticulum–Golgi intermediary complex FBS, fetal bovine serum genetic material global pandemic globe Golgi human cell human cells inaccessible inhibitor Laboratory LAMP1, lysosomal associated membrane protein 1 majority mechanism membrane bilayer MERS coronaviruses MERS-CoV, Middle East respiratory syndrome coronavirus ORFs, open reading frames pandemic PFA, paraformaldehyde producing Research resource SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 research SARS-CoV-2 viral SARS-CoV-2 VLP SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 SEM, scanning electron microscopy Spread structural protein TEM, transmission electron microscopy viral life cycle Viral protein Virology virus virus assembly virus budding virus entry Virus-like particle virus-like particle (VLP) VLPs VLPs, Virus-like particles WGA, wheat germ agglutinin Wuhan, China [DOI] 10.1074/jbc.RA120.016148 PMC 바로가기 [Article Type] Research Article
Identification of a Membrane Binding Peptide in the Envelope Protein of MHV CoronavirusArticle Published on 2020-09-222022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] Amino acid Antigen binding Cell cholesterol coding sequence coronavirus CoV CoVs distribution DPPC E protein Endoplasmic reticulum envelope envelope protein ERGIC expressing expression full-length function Giant Golgi GUV identification identify in silico insect cells intestinal mammal mammalian membrane MHV mouse mutant Neurological disease peptide peptides positive predicted Replication RNA virus sphingomyelin tested Vesicle virus were expressed wild type [DOI] 10.3390/v12091054 PMC 바로가기 [Article Type] Article