SOLVx therapeutics vaccine – Activate T-cell immunity using broad surveillance epitope strategy against mutant strains SARS-COV2SOLVx 치료제 백신 - 돌연변이 균주 SARS-COV2에 대한 광범위한 감시 에피토프 전략을 사용하여 T 세포 면역 활성화Article Published on 2022-08-012022-09-11 Journal: Biomedicine & pharmacotherapy = Biomédecine & phar [Category] COVID19(2023년), SARS, 변종, [키워드] Alpha Analysis Anti-viral B cell B cells BALB/c mice biochemical biological samples cellular coronavirus cytokine levels Cytokine storm decrease Defense develop dose Effect Efficacy and safety elevated epitope eradication euthanized Evolution functional Genome HCoV HCoV-229E hematological humoral IFN-γ IL-10 IL-6 immune response Immunoglobulin Immunoglobulin G increase in interferon gamma Laboratory lung tissue Lungs mice mRNA mutable mutant mutant strain NK cell parameter PBMC PBMCs peptide peptides Prophylactic qPCR reduced SARS-COV2 mutant significantly SOLVx statistically significant suggested Surveillance T cell T-cell immunity therapeutic TNF-alpha TNF-α treated Treatment treatment group treatment groups Vaccine vaccine. virus Virus evolution virus infectivity were assessed were used [DOI] 10.1016/j.biopha.2022.113212 PMC 바로가기 [Article Type] Article
Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs Research Published on 2022-07-252022-10-05 Journal: Nature Genetics [Category] COVID19(2023년), SARS, 유전자 메커니즘, [키워드] Activation acute respiratory syndrome AP1G1 ATP8B1 Caco-2 Calu-3 Cell cells conducted coronavirus CRISPR Factor Factors genome-wide CRISPR screen HCoV-229E HCoV-NL63 highlighting Host human cell lines inhibitors lung cell MERS-CoV Meta-analysis Middle East modulate mucins performed plasma membrane Replication respiratory syndrome coronavirus SARS-CoV-2 SARS-CoV-2 replication specificity target therapeutic interventions TMPRSS2 viral entry [DOI] 10.1038/s41588-022-01110-2 [Article Type] Research
Distinct Antibody Responses to Endemic Coronaviruses Pre- and Post-SARS-CoV-2 Infection in Kenyan Infants and Mothers케냐 유아와 산모의 SARS-CoV-2 감염 전 및 후 풍토성 코로나바이러스에 대한 독특한 항체 반응Article Published on 2022-07-122022-09-12 Journal: Viruses [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] alphacoronavirus antibody Antibody binding association Betacoronavirus betacoronavirus boosting cause Cohort coronavirus COVID-19 cross-react cross-reactive cross-reactive antibody cross-reactivity Endemic endemic human coronavirus Evidence HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 highly pathogenic IgG IgG antibody titer Infant Infants Kenya lack levels of antibody memory response mother mothers. response risk SARS-CoV-1 SARS-CoV-1 infection SARS-CoV-2 SARS-CoV-2 antibodies SARS-COV-2 infection Seroconversion unlikely women [DOI] 10.3390/v14071517 PMC 바로가기 [Article Type] Article
Structure, receptor recognition, and antigenicity of the human coronavirus CCoV-HuPn-2018 spike glycoproteinArticle Published on 2022-06-232023-06-20 Journal: Cell [Category] Fulltext, MERS, [키워드] aminopeptidase ɑ-coronaviruses. CCoV-HuPn-2018 cryo-EM HCoV-229E sialosides Zoonotic viruses [DOI] 10.1016/j.cell.2022.05.019 PMC 바로가기
Discovery and Use of Long dsRNA Mediated RNA Interference to Stimulate Antiviral Protection in Interferon Competent Mammalian Cells인터페론 유능한 포유동물 세포에서 항바이러스 보호를 자극하기 위한 긴 dsRNA 매개 RNA 간섭의 발견 및 사용Article Published on 2022-05-062022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), SARS, 치료법, 치료제, [키워드] Antiviral Antiviral effect antiviral response antiviral responses cell line cells cellular cleaved comparable competent complementary Concentration cornerstone CoV dependent on Dicer dsRNA Evidence explanation expressing fluorescent functional gene targeting generate green fluorescent protein HCoV HCoV-229E human coronavirus human coronaviruses IFN immune defense induce inducer inhibit Interference interferons knockdown Lineage Long long dsRNA mammalian mammalian cell mammalian cells Mediated mRNA pathway platform preventing infection produced protection Protein provide required responsible retained RISC RNA RNA interference RNA-induced silencing complex RNAi RNAi pathway SARS-CoV-2 replication sequence shown significantly siRNA siRNAs stimulate stomatitis suppress therapeutic These cells threshold type I interferon vesicular stomatitis vesicular stomatitis virus viral inhibition. virus VSV-GFP [DOI] 10.3389/fimmu.2022.859749 PMC 바로가기 [Article Type] Article
Repurposing dyphylline as a pan-coronavirus antiviral therapy범 코로나 바이러스 항 바이러스 요법으로 디필린 용도 변경Article Published on 2022-05-012022-09-11 Journal: Future Medicinal Chemistry [Category] COVID19(2023년), SARS, 신약개발, 치료제, [키워드] Alpha Antiviral antiviral agent antiviral therapy bind catalytic dyad Cell culture coronavirus COVID-19 pandemic CoVs Cystine Drug discovery dyphylline FDA-approved drug functional enzyme functional enzymes HCoV HCoV-229E HCoV-NL63 HCoV-OC43 HCoVs highly pathogenic histidine Human human coronavirus human coronaviruses human coronaviruses (HCoVs) humans inhibited life cycle Mild MPro NL63 OC43 protease repurposing repurposing. respiratory diseases SARS-CoV-2 severe respiratory disease severe respiratory diseases structures Support targets therapeutically target viral replication Virtual screening virus Virus life cycle viruses xanthine zoonotic [DOI] 10.4155/fmc-2021-0311 PMC 바로가기 [Article Type] Article
Enoxaparin augments alpha-1-antitrypsin inhibition of TMPRSS2, a promising drug combination against COVID-19Enoxaparin은 COVID-19에 대한 유망한 약물 조합인 TMPRSS2의 알파-1-항트립신 억제를 증가시킵니다.Article Published on 2022-03-252022-09-11 Journal: Scientific Reports [Category] SARS, 변종, 진단, 치료법, [키워드] affected alpha-1-antitrypsin anti-inflammatory activity antitrypsin Cell cells cleave complex consequence Coronavirus infection COVID-19 crystal structure drug combination ENhance enoxaparin epithelial cell epithelial cells hAEC HCoV HCoV-229E HEK293T cell HEK293T cells heparin Human coronavirus human coronavirus 229E immunostaining Infection Inflammatory less molecular nafamostat Neutralizing nucleocapsid protein overexpressing Plaque assay primary human airway Primary human airway epithelial cells quantified reactive required SARS-CoV-2 SARS-CoV-2 mutations Serine serine protease severe COVID-19 Spike protein the cell the spike protein therapy TMPRSS2 TMPRSS2 inhibitor Treatment Viral viral entry virus was determined [DOI] 10.1038/s41598-022-09133-9 PMC 바로가기 [Article Type] Article
Validating and optimizing the method for molecular detection and quantification of SARS-CoV-2 in wastewater폐수에서 SARS-CoV-2의 분자 검출 및 정량화 방법 검증 및 최적화Article Published on 2022-03-152022-09-11 Journal: The Science of the total environment [Category] MERS, SARS, 진단, 치료기술, [키워드] 229E best changes in Community community infections coronavirus COVID-19 COVID-19 disease cycle threshold disease DNA evaluated fecal FIVE followed by HCoV HCoV-229E hCoV-229E. Human coronavirus Infection Isolation less methodology Mild molecular Neutral parameter PCR PMMoV prevalence of COVID-19 provide public health qPCR quantification reported RNA extraction RNA isolation kit RT-qPCR Salmon SARS-CoV-2 SARS-CoV-2 detection sensitivity significantly significantly increased Surveillance threshold Ultrafiltration Variation Viral Viral RNA virus virus viruses was used wastewater [DOI] 10.1016/j.scitotenv.2021.151434 PMC 바로가기 [Article Type] Article
A Novel Method to Reduce ELISA Serial Dilution Assay Workload Applied to SARS-CoV-2 and Seasonal HCoVsArticle Published on 2022-03-092023-06-14 Journal: Viruses [Category] MERS, SARS, [키워드] Bayesian analysis ELISA endpoint assay HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 hierarchical modelling inflection titer SARS-CoV-2 [DOI] 10.3390/v14030562 PMC 바로가기 [Article Type] Article
Integrated hepatic single-cell RNA sequencing and untargeted metabolomics reveals the immune and metabolic modulation of Qing-Fei-Pai-Du decoction in mice with coronavirus-induced pneumonia통합 된 간 단일 세포 RNA 시퀀싱 및 표적화되지 않은 대사체는 코로나 바이러스-유도 폐렴과 마우스에서 Qing-Fei-Pai-Du 달인의 면역 및 대사 조절을 보여준다.Article Published on 2022-03-012022-08-31 Journal: Phytomedicine : international journal of phytother [Category] 유전자 메커니즘, 치료제, [키워드] alteration Analysis attenuate B cells BALB/c mice best CD4 CD8 clinically Cold Cold-damp environment Combination Control control group coronavirus coronavirus disease Coronavirus disease 2019 Coronavirus pneumonia COVID-19 patients Cytokines damp environment disease disease-associated exposure to Gene Expression HCoV HCoV-229E Human coronavirus IFN-γ IL-6 immune immune function Immunity immunological IMPROVE indicated infecting Infection Inflammation Inflammatory cytokine Inflammatory cytokines knowledge Liver injury liver tissue Lymphocytes mechanism mechanism of action metabolic function metabolism metabolomics mice Model modulate modulation mouse model Mouse models performed Peripheral blood peripheral blood lymphocyte peripheral blood lymphocytes Pneumonia profile purine Qing-Fei-Pai-Du decoction reduce regulated Regulatory reveal RNA RNA sequencing significantly significantly increased Single-cell RNA sequencing status T cells TCM therapy TNF-α transcriptional treat treated Treatment Untargeted metabolomics. [DOI] 10.1016/j.phymed.2021.153922 PMC 바로가기 [Article Type] Article