SARS-CoV-2 Pandemic Tracing in Italy Highlights Lineages with Mutational Burden in Growing Subsets이탈리아의 SARS-CoV-2 전염병 추적, 증가하는 하위 집합에서 돌연변이 부담이 있는 계보 강조Article Published on 2022-04-092022-09-11 Journal: International Journal of Molecular Sciences [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] Amino acid amino acid change analysed Analysis appearance burden clades country COVID-19 pandemic database detect develop Evolution genomic Helicase help Italian patient Italian patients Italy management methyltransferase modulation MONITOR mutated Nsp12 Nsp3 nsp4 NSP6 ORF1ab ORF3a ORF8 pandemic Positive selection RdRP SARS-CoV-2 selection pressure Spike proteins Spread spreading Structural variation subpopulation tracing trees variant variant of concern (VOC) variants Viral proteins viral sequence viral sequences viral variant viral variants tracing. virus variant virus variants VoC [DOI] 10.3390/ijms23084155 PMC 바로가기 [Article Type] Article
Biochemical and Biophysical Characterisation of the Hepatitis E Virus Guanine-7-MethyltransferaseArticle Published on 2022-02-232023-08-30 Journal: Molecules [Category] E형 간염, [키워드] enzyme assay Fluorescence quenching hepatitis E virus methyltransferase protein stability protein–ligand interaction [DOI] 10.3390/molecules27051505 PMC 바로가기 [Article Type] Article
Targeting Viral Methyltransferases: An Approach to Antiviral Treatment for ssRNA VirusesReview Published on 2022-02-122023-08-29 Journal: Viruses [Category] E형 간염, [키워드] KDKE tetrad methyltransferase methyltransferase inhibitors [DOI] 10.3390/v14020379 PMC 바로가기 [Article Type] Review
Coronaviral RNA-methyltransferases: function, structure and inhibitionAcademicSubjects/SCI00010 Published on 2022-01-252022-10-31 Journal: Nucleic Acids Research [Category] COVID-19, [키워드] Activation antiviral immunity complex Coronaviral COVID cryo-EM structure cytoplasm Degradation enzyme inhibitor inhibitors innate immune system Innate immunity methyltransferase nsp14 pandemic RNA scrutiny stability viral replication Viral RNA X-ray [DOI] 10.1093/nar/gkab1279 PMC 바로가기 [Article Type] AcademicSubjects/SCI00010
Activation of the SARS-CoV-2 NSP14 3′–5′ exoribonuclease by NSP10 and response to antiviral inhibitorsResearch Article Published on 2021-12-202022-10-30 Journal: The Journal of Biological Chemistry [Category] COVID-19, SARS, [키워드] activate acute respiratory syndrome Analysis antiviral inhibitor antiviral therapeutics biochemical C-terminal complex coronavirus COVID-19 COVID-19, Coronavirus disease 2019 CV, column volume DDEE, residues forming the active site aspartic acid (D) and glutamic acid (E) DMSO, dimethyl sulfoxide DSF, differential scanning fluorimetry ENhance enzyme enzyme kinetics Escherichia coli exonuclease exonucleases Exoribonuclease expression Factor FL, full-length highlight increase in inhibit inhibitor methyltransferase monophosphate MP, monophosphate MTase, methyltransferase N-terminal Ni, nickel nonstructural protein 14 NP-40, Nonidet-P40 nsp10 nsp14 NSP14, nonstructural protein 14 NTA, nitrilotriacetic acid observé producing Protein protein complex protein drug interaction Protein purification protein stability protein–protein interaction Proteins protocol provide purification Remdesivir Replication required RMP, remdesivir monophosphate SAH, S-adenosylhomocysteine SARS-CoV-2 SARS-CoV-2 replication SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 SEC, size-exclusion chromatography small molecule TCEP, Tris(2-carboxyethyl)phosphine the SARS-CoV-2 the SARS-CoV-2 genome Treatment understanding viral proofreading Viral protein [DOI] 10.1016/j.jbc.2021.101518 PMC 바로가기 [Article Type] Research Article
Discovery of SARS-CoV-2 Nsp14 and Nsp16 Methyltransferase Inhibitors by High-Throughput Virtual ScreeningArticle Published on 2021-11-302022-10-28 Journal: Pharmaceuticals [Category] COVID-19, [키워드] acute respiratory syndrome analogue Antiviral agents antiviral drugs Cell Compound coronavirus cytotoxic effects docking energy transfer Enzymes evade evaluated fluorescent glycine High-throughput virtual screening homogeneous HTRF Human immune system inhibition activity inhibitor inhibitors majority methyltransferase mRNA MTase inhibitors nsp14 nsp16 performed S-adenosylmethionine SAM SARS-CoV-2 Screening Seven starting point target tested the SARS-CoV-2 Virtual screening [DOI] 10.3390/ph14121243 PMC 바로가기 [Article Type] Article
Structure–function analysis of the nsp14 N7–guanine methyltransferase reveals an essential role in Betacoronavirus replication423 Published on 2021-11-292022-10-29 Journal: Proceedings of the National Academy of Sciences of [Category] COVID-19, [키워드] acute respiratory syndrome affecting Analysis approach Betacoronavirus betacoronaviruses biochemical C-terminal catalytic cellular characterized conserved coronavirus CoV CoVs Critical defined Degradation develop domain drugs enzymatic activity enzyme Exoribonuclease feature genomes highlight host cell cytoplasm identify in silico in viral inhibitor innate immune involved MERS-CoV Messenger RNA methyltransferase Middle East MOST mRNA mRNA capping mRNAs Mutation N-terminal nonstructural protein 14 nsp14 nsp14-ExoN pandemic phenotype PROTECT replicate Replication replicative residue reveal RNA synthesis SARS-CoV SARS-CoV-2 selected Significance site-directed mutagenesis Substitution subunit syndrome targets tested translation unique viability Virological virus was used yielding [DOI] 10.1073/pnas.2108709118 PMC 바로가기 [Article Type] 423
Molecular docking and pharmacokinetic studies of phytocompounds from Nigerian Medicinal Plants as promising inhibitory agents against SARS-CoV-2 methyltransferase (nsp16)Research Published on 2021-11-092022-10-30 Journal: Journal of Genetic Engineering & Biotechnology [Category] COVID-19, [키워드] Anacardium Analysis binding affinity Cell cells China circulation COVID-19 Crinum death docking docking score dose drug design drug-likeness Efficacy identify in silico in vitro in vivo index case inhibit SARS-CoV-2 inhibitory inhibitory activity Innate immunity Ligand mechanisms methyltransferase Molecular docking study molecular target more difficult nsp16 performed pharmacokinetic pharmacokinetics Phytocompounds plant Prevent quercetin Replication reported Result Salvia officinalis SARS-CoV-2 SARS-CoV-2 viral RNA stability supplementary material survival therapeutic agent unique Vaccine variants of SARS-CoV-2 virulent virus detection [DOI] 10.1186/s43141-021-00273-5 PMC 바로가기 [Article Type] Research
An antibody-based proximity labeling map reveals mechanisms of SARS-CoV-2 inhibition of antiviral immunityResearch article Published on 2021-10-222022-10-05 Journal: Cell chemical biology [Category] SARS, 치료제, [키워드] ACE2 Activation acute respiratory syndrome angiotensin-converting enzyme 2 Antiviral antiviral immunity biotinylated caused Clinical treatment Compound coronavirus coronavirus disease Coronavirus-2 COVID-19 domain drug Epidemic Human human cell humans identify immune responses Infection inhibit Interaction interactome ITGB1 knowledge labeling lysine MAVS mechanism membrane protein methyltransferase mitochondrial mitochondrial antiviral-signaling protein pathway Protein provide proximal proximity labeling replication of SARS-CoV-2 resource reveal SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 protein SET SETD2 significantly the interferon Treatment TurboID viral infection Viral protein [DOI] 10.1016/j.chembiol.2021.10.008 [Article Type] Research article
Search for RNA aptamers against non-structural protein of SARS-CoV-2: Design using molecular dynamics approachResearch Published on 2021-10-122022-10-28 Journal: Beni-Suef University Journal of Basic and Applied Sciences [Category] COVID-19, [키워드] Activation antiviral target approach aptamer binding binding affinity complex conformational docking downstream Dynamics effective ENCODE enzyme function Genome H-bond HADDOCK score Host identify investigated lack less methyltransferase molecular molecular dynamics Molecular dynamics simulation mRNA non-structural protein nsp10 nucleocapsid outbreak Protein recent replicase polyproteins respiratory Result reveal RNA RNA genome SARS-CoV-2 screened Spike proteins stability supplementary material susceptible the disease turn Vaccine Viral RNA virus particle [DOI] 10.1186/s43088-021-00152-5 PMC 바로가기 [Article Type] Research