Evolutionary dynamics of SARS-CoV-2 circulating in Yogyakarta and Central Java, Indonesia: sequence analysis covering furin cleavage site (FCS) region of the spike protein족자카르타와 인도네시아 중부 자바에서 순환하는 SARS-CoV-2의 진화 역학: 스파이크 단백질의 푸린 절단 부위(FCS) 영역을 포괄하는 서열 분석Article Published on 2022-08-012022-09-11 Journal: International microbiology : the official journal [Category] COVID19(2023년), SARS, 변종, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus Analysis cDNA cDNA synthesis circulating clade Cluster Clusters confirmed COVID-19 patients coronavirus COVID-19 pandemic COVID-19 patient D614 variant D614G D614G mutation evolutionary dynamics FCS FIVE furin furin cleavage furin cleavage site G614 variant Genome GISAID indicated Indonesia Mutation Nasopharyngeal swab nasopharyngeal swab sample Nasopharyngeal swab samples new SARS-CoV-2 new virus PCR amplification Protein Q677H Region replaced responsible retrieved RNA SARS-CoV-2 SARS-CoV-2 isolate SARS-CoV-2 isolates sequence Sequence analysis Sequencing severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 silent spike Spike protein spike protein. synthesis the spike protein were used [DOI] 10.1007/s10123-022-00239-8 PMC 바로가기 [Article Type] Article
Evaluation of a fully closed real time PCR platform for the detection of SARS-CoV-2 in nasopharyngeal swabs: a pilot study비인두 면봉에서 SARS-CoV-2 검출을 위한 완전 폐쇄형 실시간 PCR 플랫폼 평가: 파일럿 연구Article Published on 2022-08-012022-09-11 Journal: Journal of Clinical Pathology [Category] SARS, 치료기술, [키워드] addition analysed approach approach clinical follow-up close contact contact with COVID-19 cycle threshold demonstrated develop Diagnosis diagnosis of COVID-19 evaluate FIVE Follow-up gold gold standard infection control infection control. initial nasopharyngeal Nasopharyngeal swab nasopharyngeal swab sample Nasopharyngeal swab samples nasopharyngeal swabs Patient PCR pilot study platform positive positive result real time retrieved reverse transcriptase reverse transcriptase PCR robust RT-PCR RT-PCR test SARS-CoV-2 SARS-COV-2 infection sensitivity Sensitivity and specificity specificity subset symptomatic patient Symptomatic patients Test tested viral infection with COVID-19 [DOI] 10.1136/jclinpath-2021-207516 PMC 바로가기 [Article Type] Article
Kinetics of anti-nucleocapsid IgG response in COVID-19 immunocompetent convalescent patientsCOVID-19 면역적격 회복기 환자에서 항뉴클레오캡시드 IgG 반응의 동역학Article Published on 2022-07-202022-09-11 Journal: Scientific Reports [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, 치료법, [키워드] absence anti-Nucleocapsid IgG anti-SARS-CoV-2 anti-SARS-CoV-2 humoral antibody Antibody Response Antibody testing BMI comprehension convalescent patient Convalescent patients country COVID-19 COVID-19 convalescent patient COVID-19 disease decrease detectable develop disease severity humoral humoral immune response IgG IgG antibodies IgG antibody immunodeficiency inclusion criteria Kinetics knowledge longitudinal study majority Nasopharyngeal swab nasopharyngeal swab sample Nasopharyngeal swab samples nucleocapsid Patient positive Protein Re-infection robust RT- PCR SARS-CoV-2 SARS-CoV-2 nucleocapsid serum Sex Treatment Vaccination strategies Vaccination strategy virus infection [DOI] 10.1038/s41598-022-16402-0 PMC 바로가기 [Article Type] Article
No molecular evidence of SARS-CoV-2 infection in companion animals from Veracruz, Mexico멕시코 베라크루즈의 반려동물에서 SARS-CoV-2 감염의 분자적 증거 없음Article Published on 2022-07-012022-09-11 Journal: Transboundary and emerging diseases [Category] COVID19(2023년), SARS, 치료기술, [키워드] Active surveillance acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 approach cats Chain Reaction close contact collected coronavirus coronavirus COVID-19 detect dogs epidemiological epidemiological surveillance Evidence exposure to human populations imperative infectious agent infectious agents Latin America limit measure molecular N1 and N2 Nasopharyngeal swab nasopharyngeal swab sample oral organism Organisms pathogen Pathogens patients with COVID-19 polymerase chain polymerase chain reaction Population Prevent qPCR recent reduce reverse transcription reverse transcription polymerase chain reaction risk of transmission RNA RT-qPCR SARS-CoV-2 SARS-COV-2 infection severe acute respiratory syndrome Coronavirus shown species Surveillance susceptible tested Total total RNA Transmission Transmission dynamics transmitted viral gene viral genes viral infection Viral infection. was performed [DOI] 10.1111/tbed.14153 PMC 바로가기 [Article Type] Article
A method comparison study of the high throughput automated HISCL® SARS-CoV-2 antigen assay using nasopharyngeal swab samples from symptomatic and asymptomatic subjects against conventional RT-PCR기존의 RT-PCR에 대한 증상 및 무증상 피험자의 비인두 면봉 샘플을 사용한 고속 자동화 HISCL® SARS-CoV-2 항원 분석의 방법 비교 연구Article Published on 2022-07-012022-09-11 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 변종, [키워드] 95% CI 95% confidence interval 95%CI acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Antigen automated Chain Reaction collected compatibility coronavirus cycle threshold evaluate healthcare worker Healthcare workers HISCL automated antigen assay individual Infection medium method comparison Nasopharyngeal swab nasopharyngeal swab sample Nasopharyngeal swab samples polymerase chain polymerase chain reaction public health rapid testing RT-PCR SARS-CoV-2 SARS-CoV-2 antigen sensitivity severe acute respiratory syndrome Coronavirus specificity subject Swab swabs symptomatic and asymptomatic tested Transport transport medium Universal Transport Medium UTM variants variants of concern variants of concern. viral loads viral spread yielding [DOI] 10.1002/jmv.27679 PMC 바로가기 [Article Type] Article
Comparison of extraction-based and elution-based polymerase chain reaction testing, and automated and rapid antigen testing for the diagnosis of severe acute respiratory syndrome coronavirus 2중증급성호흡기증후군 코로나바이러스 2 진단을 위한 추출기반 및 용출기반 중합효소연쇄반응검사와 자동화 및 신속항원검사의 비교Article Published on 2022-07-012022-09-11 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplification amplified Antigen antigen detection applied assays automated Chain Reaction clinical sample clinical samples Concentration coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 Diagnosis diagnostic diagnostic test Dilution dilutions disease Elecsy Elecsys elution extraction Fuji Inc. Nasopharyngeal swab nasopharyngeal swab sample Nasopharyngeal swab samples Novel coronavirus novel coronavirus disease novel coronavirus disease 2019 (COVID-19) Patient PCR PCR assay PCR test performed polymerase chain polymerase chain reaction positive Rapid diagnostic test RDT remained RNA RNA extraction Roche Diagnostics SARS-CoV SARS-CoV-2 SARS-CoV-2 antigen Science severe acute respiratory syndrome Coronavirus Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). System Takara Takara Bio virus [DOI] 10.1002/jmv.27709 PMC 바로가기 [Article Type] Article
Indiscriminate SARS-CoV-2 multivariant detection using magnetic nanoparticle-based electrochemical immunosensing자성 나노입자 기반 전기화학적 면역 센싱을 이용한 무차별 SARS-CoV-2 다변량 검출Article Published on 2022-06-012022-09-11 Journal: Talanta [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] Accuracy Alpha Anti-S1 anti-S2 anti-SARS-CoV-2 antibody antibody cocktail antigen test calibration delta variant Delta variants demonstrated Developing diagnose Diagnosis diagnostic Efficiency electrochemical biosensor Frequency include Indiscriminate infected patient infected patients limit of detection Magnetic nanoparticles mitigate monoclonal monoclonal antibodies monoclonal antibody Mutation nasopharyngeal Nasopharyngeal swab nasopharyngeal swab sample Nasopharyngeal swab specimens nasopharyngeal swabs optimization pandemic performed platform polyclonal polyclonal antibody positive positive sample RT-PCR SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2 virus SARS-CoV-2. sensitivity specificity Swab tested the SARS-CoV-2 virus variant variants variants of SARS-CoV-2 [DOI] 10.1016/j.talanta.2022.123356 PMC 바로가기 [Article Type] Article
A fast extraction-free isothermal LAMP assay for detection of SARS-CoV-2 with potential use in resource-limited settings자원이 제한된 환경에서 잠재적으로 사용할 수 있는 SARS-CoV-2 검출을 위한 빠른 추출이 필요 없는 등온 LAMP 분석Article Published on 2022-05-022022-09-11 Journal: Virology Journal [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, [키워드] alternative method analysed Analysis caused clinical sample clinical samples conditions country current demonstrated determine disease equipment extracted RNA extraction Extraction-free LAMP IMPROVE Incubation isothermal isothermal amplification LAMP limit of detection London management Nasopharyngeal swab nasopharyngeal swab sample Nasopharyngeal swab samples non-specific not affect nucleic acid PCR phosphate Phosphate Buffered Saline primer pair primer pairs qRT-PCR qRT-PCR. Quantitative reaction reagents resource resource-limited settings RLS RNA RT-PCR RT-PCR assays SARS-CoV-2 Sensitivity and specificity significantly Spread Street suggested tested Viral viral transport media were used [DOI] 10.1186/s12985-022-01800-7 PMC 바로가기 [Article Type] Article
Bi-ECDAQ: An electrochemical dual-immuno-biosensor accompanied by a customized bi-potentiostat for clinical detection of SARS-CoV-2 Nucleocapsid proteinsBi-ECDAQ: SARS-CoV-2 뉴클레오캡시드 단백질의 임상 검출을 위한 맞춤형 이중-전위기(bi-potentiostat)와 함께 제공되는 전기화학적 이중 면역 바이오센서Article Published on 2022-05-012022-09-11 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), SARS, 치료기술, 치료법, [키워드] accompanied acquisition active site active sites All-in-one data acquisition platform approach Bi-potentiostat readout system carboxyl comparable complex COVID-19 COVID-19 patient Data acquisition diagnostics Diseases Dual working screen-printed electrode Electrochemical immunosensing eliminating flexible functional incompatible lack limit of detection matrix effect N-protein Nasopharyngeal swab nasopharyngeal swab sample nucleocapsid protein offered performed point-of-care Protein Recognition resulting SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 nucleocapsid protein. Screen sensitive assay sensitivity significantly lower unmet need [DOI] 10.1016/j.bios.2022.114018 PMC 바로가기 [Article Type] Article
SARS-CoV-2 electrochemical immunosensor based on the spike-ACE2 complex스파이크-ACE2 복합체 기반 SARS-CoV-2 전기화학 면역센서Article Published on 2022-05-012022-09-11 Journal: Analytica chimica acta [Category] COVID19(2023년), SARS, 치료기술, [키워드] ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 angiotensin Angiotensin-converting enzyme anti-spike antibody antibody buffer complex coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 detect Diagnosis enzyme gold healthy individual horseradish peroxidase host receptor HRP immunosensor infected patient infected patients Interaction limit of detection magnetic bead Magnetic beads measure mediator mitigate Nasopharyngeal swab nasopharyngeal swab sample Pandemics Particle Particles peroxidase proof Protein pseudovirions Rapid reporter reproducibility resulting Sandwich SARS-CoV-2 sensitivity severe acute respiratory syndrome Coronavirus spike Spike protein spike protein. the patient the spike protein viral particle Viral particles virus was recorded [DOI] 10.1016/j.aca.2022.339718 PMC 바로가기 [Article Type] Article