Comparison of reverse-transcription qPCR and droplet digital PCR for the detection of SARS-CoV-2 in clinical specimens of hospitalized patients입원환자 임상검체에서 SARS-CoV-2 검출을 위한 역전사 qPCR과 droplet digital PCR 비교Article Published on 2022-06-012022-09-11 Journal: Diagnostic microbiology and infectious disease [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition alternative caused Chain Reaction China clinical Clinical performance coronavirus coronavirus disease Coronavirus disease 2019 correlated COVID-19 Critical ddPCR demonstrated detect Diagnosis Digital digital PCR discharged evidenced Hospitalized hospitalized patient Huoshenshan Hospital N gene negative ORF ORF gene oropharyngeal swab Patient patients patients hospitalized PCR polymerase chain polymerase chain reaction qPCR Quantitative Quantitative detection recovered patients Result reverse transcription risk RT-qPCR SARS-CoV-2 severe acute respiratory syndrome Coronavirus significantly specimen target gene Target genes the patient Treatment viral gene viral genes Viral load viral shedding Wuhan [DOI] 10.1016/j.diagmicrobio.2022.115677 PMC 바로가기 [Article Type] Article
Contact tracing period and epidemiological characteristics of an outbreak of the SARS-CoV-2 Delta variant in Guangzhou광저우 SARS-CoV-2 Delta 변종 발병의 접촉자 추적 기간 및 역학적 특성Article Published on 2022-04-012022-09-11 Journal: International Journal of Infectious Diseases [Category] COVID19(2023년), SARS, 변종, [키워드] adjusted adjusted odds ratio attack rate Characteristics close contact collected Contact Contact tracing contact with COVID-19 cycle cycle threshold Delta delta variant effective epidemiological epidemiological characteristics. exposure time exposure to Frequency Guangzhou household contact household contacts identify Incubation period index case index cases N gene occurred ORF outbreak presymptomatic transmission Prevention strategy risk SARS-CoV-2 SARS-CoV-2 delta secondary attack rate symptom onset the SARS-CoV-2 threshold time interval Transmission Transmission dynamics [DOI] 10.1016/j.ijid.2022.01.034 PMC 바로가기 [Article Type] Article
Electrochemical biosensor for nucleic acid amplification-free and sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA via CRISPR/Cas13a trans-cleavage reactionCRISPR/Cas13a trans-cleavage 반응을 통한 중증 급성 호흡기 증후군 코로나바이러스 2(SARS-CoV-2) RNA의 핵산 증폭이 없고 민감한 검출을 위한 전기화학 바이오센서Article Published on 2022-04-012022-09-11 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), SARS, 치료기술, 치료법, [키워드] activated acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 artificial saliva biosensor cleave complex Concentration coronavirus COVID-19 COVID-19 pandemic CRISPR CRISPR/Cas13a detect diagnostic diagnostic testing dynamic range electrochemical biosensor Epidemic evaluated fatality Genes gold identify introduced nucleic acid occur ORF outbreak probe RNA RNase Saliva SARS-CoV-2 SARS-CoV-2 RNA severe acute respiratory syndrome Coronavirus ssRNA Trans-cleavage activity. Transmission Trigger virus detection [DOI] 10.1016/j.bios.2021.113960 PMC 바로가기 [Article Type] Article
Comparative Analysis of SARS-CoV-2 Variants of Concern, Including Omicron, Highlights Their Common and Distinctive Amino Acid Substitution Patterns, Especially at the Spike ORF오미크론을 포함한 우려되는 SARS-CoV-2 변이체의 비교 분석은 특히 스파이크 ORF에서 공통적이고 독특한 아미노산 치환 패턴을 강조합니다Comparative Study Published on 2022-03-292022-09-11 Journal: Viruses [Category] SARS, 변종, [키워드] acid Alpha Amino acid amino acid substitution amino acid substitutions Analysis Beta common Comparative concern COVID-19 Delta dN/dS Evolution Gamma highest homologous hotspot human host Hypothesis Including introduced investigated Lambda Lineage lineages mutated mutations omicron ORF phylogenetic distribution Point mutation Positive selection RBD Receptor binding domain Recombination recurrent mutations residue Sarbecoviruses SARS-CoV-2 significantly higher spike Substitution the Spike vaccination variant variants variants of concern was performed [DOI] 10.3390/v14040707 PMC 바로가기 [Article Type] Comparative Study
Assessment of SARS-CoV-2 RNA shedding in semen of 36 males with symptomatic, asymptomatic, and convalescent infection during the first and second wave of COVID-19 pandemic in Italy이탈리아에서 COVID-19 대유행의 1차 및 2차 파동 동안 증상, 무증상 및 회복기 감염이 있는 36명의 남성 정액에서 SARS-CoV-2 RNA 배출 평가Article Published on 2022-03-012022-09-11 Journal: Asian journal of andrology [Category] SARS, 치료기술, 치료제, [키워드] ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus-2 angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 assessment Asymptomatic Chain Reaction Clinical practice convalescent coronavirus coronavirus disease Coronavirus disease-19 Coronavirus-2 COVID-19 COVID-19 pandemic defined detectable detection determine Diagnosis enrolled Evidence evidence of Frame Infection interview Italy male medical history men Mild Nasopharyngeal swab nasopharyngeal swabs nested PCR no evidence of no symptom Open reading frame ORF Patient PCR persistence polymerase chain reaction positive public health real-time reverse transcriptase-polymerase chain reaction real-time RT-PCR receptor recovering from COVID-19 Result reverse transcriptase reverse transcriptase-polymerase chain reaction risk of transmission RT-PCR sampling SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 RNA second wave Semen Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus sexual intercourse sexual transmission symptomatic Symptoms was performed [DOI] 10.4103/aja2021103 PMC 바로가기 [Article Type] Article
Consecutive deletions in a unique Uruguayan SARS-CoV-2 lineage evidence the genetic variability potential of accessory genes독특한 우루과이 SARS-COV-2 계보의 연속 결실Research Article Published on 2022-02-172022-09-10 Journal: PLoS ONE [Category] COVID19(2023년), MERS, SARS, 변종, [키워드] accessory gene Accessory genes Amino acid amino acids B.1.1.33 capillary electrophoresis changes changes in clade collected Complete Deletion deletions downstream Evidence function fused fused protein genetic variability Genetic variation Genetic variations Genome genomic help Illumina include individual Lineage Next-generation sequencing nucleotide nucleotides occur Occurrence ORF ORF7a ORF7b peptide promote Protein Sanger sequencing SARS-CoV-2 SARS-CoV-2 lineage signal peptide the early SARS-CoV-2 the signal peptide transmembrane domain transmembrane protein unique virus [DOI] 10.1371/journal.pone.0263563 PMC 바로가기 [Article Type] Research Article
SARS-CoV-2 wastewater surveillance in Germany: Long-term RT-digital droplet PCR monitoring, suitability of primer/probe combinations and biomarker stability독일의 SARS-CoV-2 폐수 감시: 장기 RT-디지털 액적 PCR 모니터링, 프라이머/프로브 조합의 적합성 및 바이오마커 안정성Case Reports Published on 2022-02-152022-09-11 Journal: Water research [Category] COVID19(2023년), MERS, SARS, 바이오마커, 변종, 치료기술, 치료제, [키워드] approach assays B.1.1.7 Biomarker Biomarker stability Case report circulation city Clinical data Clinical tests Combination Concentration conditions contributing to copy numbers coronavirus correlation COVID-19 case detect determine Digital Digital droplet PCR dissolved oxygen Early detection early stage Epidemiology experiment Germany glycol management methodology N501Y N501Y mutation Nsp3 Nsp9 Occurrence ORF ORF1 ORF1ab oxygen pandemic PCR PCR analysis Polyethylene glycol primer probe proportion reported robust SARS-CoV-2 SARS-CoV-2 RNA Seven shown Spread stability Surveillance target target gene Target genes the SARS-CoV-2 titre Treatment variant variants variants of concern Viral Viral RNA was used wastewater Wastewater-based epidemiology wildtype [DOI] 10.1016/j.watres.2021.117977 PMC 바로가기 [Article Type] Case Reports
Are There Hidden Genes in DNA/RNA Vaccines?Immunology Published on 2022-02-082022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, [키워드] Accessory genes administration antigenic bioinformatics clinical outcomes codon optimization connection contribute death DNA and RNA DNA vaccine Effects examined highlight inactivated information Moderna mRNA-1273 vaccine morbidity Mortality mRNA vaccine offer Open reading frame ORF ORF-Sh ORF2b ORFs overlap overlapping Pain peptides positive predicted produced Protein Proteins reported resources respiratory RNA vaccine SARS-CoV-2 SARS-CoV-2 protein selected sequence shown Spike protein structure-function the spike protein translation Treatment utility vaccination vaccination rate Vaccine vaccine candidate Vaccine design Vaccine development Vaccines virus wild-type [DOI] 10.3389/fimmu.2022.801915 PMC 바로가기 [Article Type] Immunology
Diminished amplification of SARS-CoV-2 ORF1ab in a commercial dual-target qRT-PCR diagnostic assay상업용 이중 표적 qRT-PCR 진단 분석에서 SARS-CoV-2 ORF1ab의 증폭 감소Article Published on 2022-02-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] (RdRp) amplification Cobas Ct-value Ct-values diagnostic diagnostics diminished discrepancy Dual-target assay E-gene false-negative false-negative results genomic region highlight house limit molecular molecular diagnostics Molecular diagnostics SARS-CoV-2 nsp10 nucleotide nucleotide mutation ORF ORF1 ORF1ab ORF1ab mutations PCR PCR amplification primer probe qRT-PCR RdRP resulting SARS-CoV-2 SARS-CoV-2 PCR tests SARS-CoV-2 variant Surveillance target region target regions unique [DOI] 10.1016/j.jviromet.2021.114397 PMC 바로가기 [Article Type] Article
Detection of SARS-CoV-2 RNA in Upper Respiratory Swap Samples by Pooling Method풀링 방법에 의한 상부 호흡기 스왑 샘플에서 SARS-CoV-2 RNA 검출Article Published on 2022-01-252022-09-11 Journal: Balkan medical journal [Category] SARS, 치료기술, 치료법, [키워드] 2019-nCoV amplification analyzed appear approach Bio-rad BioRad blood test blood tests calculated Chain Reaction conducted coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 pandemic Depletion detection Diagnostic accuracy diagnostic test effective Efficiency equipment examined extraction FIVE Hercules highest hospital Inc. Laboratory lowest molecular molecular diagnostic tests nucleocapsid ORF outbreak pandemic platform pooling Population positive positive sample prevalence of COVID-19 Prospective Study public health reached reagent reagents reduce regions resource respiratory respiratory syndrome coronavirus respiratory syndrome coronavirus 2 RNA RNA extraction RT-PCR RT-PCR test RT-qPCR Sample SARS CoV-2 SARS-CoV-2 SARS-CoV-2 RNA sensitivity serial dilution serial dilutions severe respiratory syndrome standard curve tested Testing the SARS-CoV-2 threshold touch transfusion upper USA Viral load was used [DOI] 10.5152/balkanmedj.2021.21135 PMC 바로가기 [Article Type] Article