A Stabilized, Monomeric, Receptor Binding Domain Elicits High-Titer Neutralizing Antibodies Against All SARS-CoV-2 Variants of Concern안정화된 단량체 수용체 결합 도메인은 우려되는 모든 SARS-CoV-2 변이체에 대해 고역가 중화 항체를 유도합니다Immunology Published on 2021-12-092022-09-11 Journal: Frontiers in Immunology [Category] SARS, 변종, 진단, [키워드] (Beta Against antibody B.1 B.1.351 B.1.351 RBD B.1.617.2 breadth cocktail components concern COVID-19 elicited expressed generate hamster hamster immunization hamsters High-dose Host hyperstable mutants immunized immunogen immunogenic increase in introduced lack lyophilization mammalian cell mammalian cells melting temperature mice microbial Mutagenesis mutant Mutation neutralization titer neutralization titers neutralized Neutralizing antibodies Pichia pastoris produced Protein RBD room temperature SARS-CoV-2 SARS-CoV-2 spike receptor-binding domain saturation sera significantly spike receptor-binding domain squalene stabilizing mutation SWE temperature the SARS-CoV-2 thermotolerance vaccination Vaccine variants of concern Viral viral challenge VoC was used water wild-type [DOI] 10.3389/fimmu.2021.765211 PMC 바로가기 [Article Type] Immunology
Scalable, methanol‐free manufacturing of the SARS‐CoV‐2 receptor‐binding domain in engineered Komagataella phaffiiCommunication Published on 2021-11-152022-10-27 Journal: Biotechnology and bioengineering [Category] COVID-19, [키워드] Antigen approach candidate Cell clinical trial COVID‐19 COVID‐19 Elimination genetic engineering Health Host methanol microbial microbial engineering other variant Pichia pastoris platform prevention Protein Protein subunit RBD receptor‐binding domain Recombinant protein removal reported SARS‐CoV‐2 secreted secreted protein Spike protein Stress Subunit vaccine the RBD protein transfer Vaccine vaccine antigen vaccine candidate vaccine candidates Vaccines yeast [DOI] 10.1002/bit.27979 PMC 바로가기 [Article Type] Communication
Engineered SARS-CoV-2 receptor binding domain improves manufacturability in yeast and immunogenicity in mice조작된 SARS-CoV-2 수용체 결합 도메인은 효모의 제조 가능성과 마우스의 면역원성을 향상시킵니다.403 Published on 2021-09-212022-09-10 Journal: Proceedings of the National Academy of Sciences of [Category] SARS, 변종, 진단, [키워드] ACE2 Antigen approved binding binding domain Candidates Cell country COVID-19 COVID-19 pandemic domain dose doses exhibit exhibited hamster hamsters high-affinity binding immune response immunogenicity IMPROVE Intervention limit manufacturability mice microbial MOST other diseases Pichia pastoris presentation Protein protein vaccine raise raised RBD Receptor-binding domain Recombinant protein recombinant proteins reduced reported Safe SARS-CoV-2 SARS-CoV-2 receptor SARS-CoV-2 variant sequence sequences Significance single dose Subunit vaccine subunit vaccines the RBD the receptor-binding domain the SARS-CoV-2 Vaccine vaccine candidate Vaccines variant Viral viral challenge viral entry virus VLP Volume weight loss Wuhan-Hu-1 yeast [DOI] 10.1073/pnas.2106845118 PMC 바로가기 [Article Type] 403
Yeast-produced RBD-based recombinant protein vaccines elicit broadly neutralizing antibodies and durable protective immunity against SARS-CoV-2 infectionArticle Published on 2021-08-182022-10-04 Journal: Cell Discovery [Category] 비임상, [키워드] Antisera B.1.1.7 B.1.351 Biological techniques circulating variant COVID-19 pandemic dimeric domain elicit expressed feasible functional immunization immunized immunology live SARS-CoV-2 long-lasting neutralizing antibodies massive mice monomeric RBD neutralize neutralizing antibody Pichia pastoris predominant produced Protective protective immunity Protein raised RBD RBD protein recombinant protein vaccine SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 vaccine SARS-CoV-2 vaccines secreted protein the RBD vaccine dose Vaccines virus yeast [DOI] 10.1038/s41421-021-00315-9 PMC 바로가기 [Article Type] Article
SARS‑CoV-2 RBD219-N1C1: A yeast-expressed SARS-CoV-2 recombinant receptor-binding domain candidate vaccine stimulates virus neutralizing antibodies and T-cell immunity in miceSARS‑CoV-2 RBD219-N1C1: 효모 발현 SARS-CoV-2 재조합 수용체 결합 도메인 후보 백신은 마우스에서 바이러스 중화 항체 및 T 세포 면역을 자극합니다Article Published on 2021-08-032022-09-12 Journal: Human vaccines & immunotherapeutics [Category] MERS, SARS, 진단, [키워드] ACE-2 alum Antigen binding candidate vaccine Combination conducted coronavirus COVID-19 COVID-19 vaccine Cytokines domain doses elicit expressed IFN-γ IgG antibodies IL-10 IL-6 immunization immunogenicity IMPROVE in vitro mice Modification Neutralizing antibody titer Pichia pastoris produced Protein protein expression pseudovirus RBD RBD gene reduce SARS-CoV-2 SARS-CoV-2 pseudovirus secretion sera stability stimulate T-cell immunity tested the vaccine These data Vaccine vaccine candidate virus neutralizing antibody wild-type yeast [DOI] 10.1080/21645515.2021.1901545 PMC 바로가기 [Article Type] Article
Process development and scale-up optimization of the SARS-CoV-2 receptor binding domain–based vaccine candidate, RBD219-N1C1Biotechnological Products and Process Engineering Published on 2021-05-072022-10-28 Journal: Applied Microbiology and Biotechnology [Category] COVID-19, SARS, [키워드] Abstract ACE2 receptor binding addition Antigen clinical testing condition COVID-19 COVID-19 vaccine downstream elicit fermentation generate good manufacturing practice include mice Neutralizing antibody response performed Pichia pastoris Process produced Protein protocol purification purified Receptor binding recombinant COVID-19 vaccine robust SARS-CoV-2 SARS-CoV-2 pseudovirus SEC-HPLC shown Spike protein supplementary material the SARS-CoV-2 upstream vaccine candidate yeast [DOI] 10.1007/s00253-021-11281-3 PMC 바로가기 [Article Type] Biotechnological Products and Process Engineering
Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirusResearch Published on 2021-05-012024-09-02 Journal: Microbial Cell Factories [Category] 대상포진, [키워드] biomanufacturing Pichia pastoris Quality by Design Subunit vaccine [DOI] 10.1186/s12934-021-01583-6 PMC 바로가기 [Article Type] Research
Development of a platform process for the production and purification of single‐domain antibodiesArticle Published on 2021-03-252023-05-22 Journal: Biotechnology and bioengineering [Category] Fulltext, 신종인플루엔자, [키워드] Komagataella phaffii integrated purification Pichia pastoris single‐domain antibodies straight‐through chromatography VHH [DOI] 10.1002/bit.27724 PMC 바로가기
A potent neutralizing nanobody against SARS‐CoV‐2 with inhaled delivery potentialOriginal Article Published on 2021-03-042022-10-30 Journal: MedComm [Category] COVID-19, [키워드] ACE2 antiviral activity blocked Candidates coronavirus disease COVID‐19 dose Efficacy enzyme exhibited global public health highest human ACE2 immunized Inhaled Interaction large‐scale production mutants nanobody nebulization Neutralizing Neutralizing activity pandemic Pichia pastoris produced Prophylactic RBD reached receptor‐binding domain recognize reported SARS‐CoV‐2 SARS‐CoV‐2 Seven shown stability stability profile therapeutic therapeutic drug treat variant [DOI] 10.1002/mco2.60 PMC 바로가기 [Article Type] Original Article
Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cellsArticle Published on 2020-12-112022-10-29 Journal: Scientific Reports [Category] COVID-19, SARS, [키워드] acute respiratory syndrome computational analysis Coronavirus-2 expressed functional glycans Glycoproteins HEK-293T cells heterogeneity immunization indicated ionic interaction mammalian cell Neutralizing antibodies Pichia pastoris produced Protein Proteins RBD Receptor binding domain reduced SARS-CoV-2 serological test spike stability variant was increased yeast [DOI] 10.1038/s41598-020-78711-6 PMC 바로가기 [Article Type] Article