primer set – Page 2 – Communicable Diseases Research

Accuracy of Real-Time Polymerase Chain Reaction in COVID-19 Patients

COVID-19 환자의 실시간 중합효소 연쇄 반응의 정확도

Research Article

Published on 2022-02-162022-09-10
Journal: Microbiology Spectrum
[Category] COVID19(2023년), MERS, SARS, 치료기술, 치료법, 치료제,

[키워드] E gene NP gene RdRp-gene Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addressed Analysis assays caused CDC CDC N1, N2 center chain Characteristics Clinical practice clinical samples conducted Control coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 patient cutoff value cycle threshold demonstrated Diagnosis diagnostic Diagnostic accuracy diagnostic specificity discrepancy disease control enrolled followed by Health Organization highest house lowest majority Mild nasopharyngeal nasopharyngeal sample Nasopharyngeal samples nucleocapsid protein Numerous Oropharyngeal oropharyngeal samples pandemic prevention primer primer set primer sets Primers Prospective Study qPCR qPCR analysis RdRP reaction Real-time PCR real-time polymerase chain reaction Research respiratory Respiratory illness RT-PCR RT-qPCR RT-qPCRs Saliva SARS-CoV-2 SARS-CoV2 sensitivity Sensitivity and specificity Seroconversion severe acute respiratory syndrome Coronavirus severe respiratory illness significantly specificity specimen specimens sputum Sputum sample Viral Viral detection WHO World Health Organization
[DOI] 10.1128/spectrum.00591-21 PMC 바로가기 [Article Type] Research Article

Mass Screening of SARS-CoV-2 Variants using Sanger Sequencing Strategy in Hiroshima, Japan

일본 히로시마에서 Sanger 시퀀싱 전략을 사용한 SARS-COV-2 변이체의 질량 스크리닝

Article

Published on 2022-02-142022-09-01
Journal: Scientific Reports
[Category] COVID19(2023년), MERS, SARS, 변종, 진단,

[키워드] (Alpha) Alpha amplification amplified amplified product amplified products applied assigned B.1.1.529 B.1.1.7 B.1.351 B.1.617.1 B.1.617.2 Cluster confirmed case country COVID-19 develop E484K effective Effectiveness Epidemiology feasible genome sequence Genome sequences HCoV Hiroshima house identify indicate isolates Japan less mass Mass screening mutant Mutation next generation sequencing NGS notable number of sample number of samples P.1 pandemic PCR-based techniques positive Prevention and control primer primer set primer sets Primers protocol Randomly readiness reliability repeated reported RT-PCR Sanger sequencing SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants screened Screening screening strategy selected single mutation Strategy variant
[DOI] 10.1038/s41598-022-04952-2 PMC 바로가기 [Article Type] Article

Analysis of the ARTIC Version 3 and Version 4 SARS-CoV-2 Primers and Their Impact on the Detection of the G142D Amino Acid Substitution in the Spike Protein

ARTIC 버전 3 및 버전 4 SARS-CoV-2 프라이머의 분석 및 스파이크 단백질의 G142D 아미노산 치환 검출에 미치는 영향

Article

Published on 2021-12-222022-09-11
Journal: Microbiology Spectrum
[Category] SARS, 변종, 진단, 치료기술,

[키워드] absence acid addition addressed adoption Amino acid amino acid substitution amplification amplified amplify amplifying ARTIC ARTIC Network clinical sample clinical samples COVID-19 Critical D950N delta variant detection downstream downstream analyses G142D Genome Genome sequences Genome sequencing genomes Illumina Impact improvement initial iteration Laboratory Metadata mutations NovaSeq 6000 observé PCR primer sequence PCR primer sequences primer primer set primer sets Primers primers. provide recommendation reference genome Region released resource SARS-CoV-2 SARS-CoV-2 genomes sequence sequenced Sequencing sequencing depth sequencing read SNP Spike protein Spread subsequent Substitution the SARS-CoV-2 the Spike the spike protein V3 primer set variant Variation Version widespread
[DOI] 10.1128/Spectrum.01803-21 PMC 바로가기 [Article Type] Article

Simplified Point-of-Care Full SARS-CoV-2 Genome Sequencing Using Nanopore Technology

Protocol

Published on 2021-12-162022-10-29
Journal: Microorganisms
[Category] COVID-19,

A Sensitive, Portable Microfluidic Device for SARS-CoV-2 Detection from Self-Collected Saliva

Article

Published on 2021-12-142022-10-30
Journal: Infectious Disease Reports
[Category] COVID-19,

[키워드] acute respiratory syndrome approach Bronchoalveolar lavage cartridge cDNA coronavirus demonstrated detection Detection sensitivity Device diagnostic test FDA fecal samples genomic Infection integration Internal control Isolation medical intervention Microfluidics multiplex Nasopharyngeal swab Novel coronavirus outbreak pandemic point-of-care polymerase chain primer primer set protocol Quantitative quantitative PCR reverse transcription RNA RNA isolation RNA template Saliva SARS-Co-V2 SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 nucleic acid SARS-CoV-2 RNA SARS-CoV2 infection sensitive sensitivity Spread the patient Treatment Urine Viral RNA virus
[DOI] 10.3390/idr13040097 PMC 바로가기 [Article Type] Article

Multiplexed detection of respiratory pathogens with a portable analyzer in a “raw-sample-in and answer-out” manner

Article

Published on 2021-11-232022-10-04
Journal: Microsystems & Nanoengineering
[Category] 진단,

A rapid and low-cost protocol for the detection of B.1.1.7 lineage of SARS-CoV-2 by using SYBR Green-based RT-qPCR

SYBR Green 기반 RT-qPCR을 사용하여 SARS-CoV-2의 B.1.1.7 계통을 탐지하기 위한 빠르고 저렴한 프로토콜

Article

Published on 2021-11-012022-09-11
Journal: Molecular biology reports
[Category] SARS, 변종, 치료기술, 치료제,

RT qLAMP-Direct Detection of SARS-CoV-2 in Raw Sewage

미처리 하수에서 SARS-CoV-2의 RT qLAMP-직접 검출

Article

Published on 2021-09-012022-09-11
Journal: Journal of biomolecular techniques : JBT
[Category] SARS, 치료기술, 치료제,

[키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplification Analysis applicability basic Characteristics clinical sample clinical samples complex Concentration coronavirus COVID-19 incidence described detect detection downstream Health health department independent isothermal amplification LAMP Local media N-gene number of sample ORF1a primer primer set primer sets Quantitative Recognition Region reported reverse transcriptase SARS-CoV-2 SARS-CoV-2 detection selected selective severe acute respiratory syndrome Coronavirus sewage significantly target tested to define transcriptase Treatment Viral RNA virus
[DOI] 10.7171/jbt.21-32-03-016 PMC 바로가기 [Article Type] Article

Molecular Characterization of Increased Amplicon Lengths in SARS-CoV-2 Reverse Transcription Loop-Mediated Isothermal Amplification Assays

SARS-CoV-2 역전사 루프 매개 등온 증폭 분석에서 증가된 앰플리콘 길이의 분자 특성화

Article

Published on 2021-09-012022-09-11
Journal: Journal of biomolecular techniques : JBT
[Category] SARS, 유전자 메커니즘, 치료기술, 치료법, 치료제,

Optimization and validation of RT-LAMP assay for diagnosis of SARS-CoV2 including the globally dominant Delta variant

전 세계적으로 지배적 인 델타 변이체를 포함하여 SARS-COV2의 진단을위한 RT-LAMP 분석의 최적화 및 검증

Research

Published on 2021-08-302022-09-01
Journal: Virology Journal
[Category] MERS, SARS, 변종, 유전자 메커니즘, 진단,

[키워드] acute respiratory syndrome coronavirus amplification Analysis B.1.1.7 B.1.1.7 (Alpha) B.1.351 B.1.351 (Beta) B.1.427 B.1.429 (Epsilon) B.1.525 B.1.525 (Eta) B.1.526 B.1.526 (Iota) B.1.617.1 B.1.617.1 (Kappa) B.1.617.2 B.1.617.2 (Delta) causative agent condition conserved conserved regions coronavirus coronavirus 2 COVID-19 COVID-19 pandemic delta variant development Diagnosis diagnosis of COVID-19 discrimination DNA polymerase dominant double blind double blind study evaluated gold gold standard Health Organization include infected individual infected individuals Isolation isothermal LAMP Lineage Loop-mediated isothermal amplification molecular Molecular diagnosis objective optimization P.1 P.1 (Gamma) P.2 P.2 (Zeta) P.3 (Theta) pathogenic coronaviruses Patient PCR primer binding site primer binding sites primer set Primers real time PCR Region respiratory Result RT-LAMP SARS-CoV-2 SARS-CoV2 severe acute respiratory syndrome coronavirus 2 Spread supplementary material Testing the disease time variants variants of concern variants of interest Visualization VoC WHO World Health Organization
[DOI] 10.1186/s12985-021-01642-9 PMC 바로가기 [Article Type] Research