Establishment of an in-house real-time RT-PCR assay for the detection of severe acute respiratory syndrome coronavirus 2 using the first World Health Organization international standard in a resource-limited country자원이 제한된 국가에서 최초의 세계보건기구 국제표준을 이용한 중증급성호흡기증후군 코로나바이러스 2 검출을 위한 사내 실시간 RT-PCR 분석법 구축Article Published on 2022-05-012022-09-11 Journal: Journal of Clinical Laboratory Analysis [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome caused Clinical performance clinical sample clinical samples conserved coronavirus correlation country COVID-19 COVID-19 pandemic Critical E gene evaluated house International international standard limit of detection molecular molecular assays multiplex N gene Nasopharyngeal samples Nasopharyngeal swab nasopharyngeal swabs NIBSC Patient PCR assay positive Primers probe probes public health real-time RT-PCR real-time RT-PCR. reference standard Region RT-PCR assay Saliva SARS-CoV-2 specimen Standard Target genes WHO World Health Organization [DOI] 10.1002/jcla.24355 PMC 바로가기 [Article Type] Article
Meta-analysis of the robustness of COVID-19 diagnostic kit performance during the early pandemic초기 전염병 동안 COVID-19 진단 키트 성능의 견고성에 대한 메타 분석Meta-Analysis Published on 2022-04-212022-09-11 Journal: BMJ Open [Category] COVID19(2023년), SARS, 진단, 치료기술, [키워드] 95% CI approval articles chemiluminescent immunoassay complement Concordance contributed COVID-19 COVID-19 pandemic diagnose diagnosis of COVID-19 diagnostic diagnostic test diagnostic tests early phase ELISA evaluated exhibited False negative FIVE followed by Google Scholar Health heterogeneity highest highlight involved Laboratory Lateral flow immunoassays meta-analyses mitigate molecular diagnostics negative RT-PCR pandemic participant Participants patients platform positive positive RT-PCR primer Primers protocol Public public health public health. QUADAS-2 Quantitative random effect reagent reagents Region robust robustness RT-PCR RT-PCR test RT-PCR tests sampling SARS-CoV-2 sensitivity Sensitivity and specificity serology testing serology tests serum antibody specificity spread of COVID-19 synthesis Variability was done while [DOI] 10.1136/bmjopen-2021-053912 PMC 바로가기 [Article Type] Meta-Analysis
Research progress in laboratory detection of SARS-CoV-2SARS-CoV-2의 실험실 검출 연구 진행Review Published on 2022-04-012022-09-11 Journal: Irish Journal of Medical Science [Category] COVID19(2023년), SARS, 치료기술, 치료법, 치료제, [키워드] advancement analyzed antibody Antibody detection can not Characteristics clinical samples Complete complexity detection efficiency Detection sensitivity diagnosing Diagnosis disadvantage Disadvantages Efficiency false-negative false-negative results help highest Increases Laboratory Laboratory detection NCBI New coronavirus Nucleic acid detection nucleic acid testing nucleocapsid protein primer Primers Probability provided qRT-PCR ranged reported required Research SARS-CoV-2 SARS-CoV-2 detection SARSCoV-2 Serological detection. target viral infection virus was used [DOI] 10.1007/s11845-021-02604-4 PMC 바로가기 [Article Type] Review
High-Integrity Sequencing of Spike Gene for SARS-CoV-2 Variant DeterminationSARS-CoV-2 변이체 결정을 위한 스파이크 유전자의 높은 무결성 시퀀싱Article Published on 2022-03-172022-09-11 Journal: International Journal of Molecular Sciences [Category] SARS, 변종, 유전자 메커니즘, 치료기술, [키워드] amplicons approach ARTIC Network consensus sequence Coverage datasets demonstrated detect detection rate Determination determine Disadvantages evaluated Express FASTQ in silico mutations Nanopore nanopore sequencing overcome Oxford PCR PCR primer PCR primers primer primer set primer sets Primers protocol provided Region robustness SARS-CoV-2 SARS-CoV-2 genome sequence sequenced sample sequenced samples Sequencing spike spike gene Spike protein the SARS-CoV-2 genome variant Variant. variants [DOI] 10.3390/ijms23063257 PMC 바로가기 [Article Type] Article
Clinical Evaluation of a Fully-Automated High-Throughput Multiplex Screening-Assay to Detect and Differentiate the SARS-CoV-2 B.1.1.529 (Omicron) and B.1.617.2 (Delta) Lineage VariantsSARS-CoV-2 B.1.1.529(오미크론) 및 B.1.617.2(델타) 계통 변이체를 감지하고 구별하기 위한 완전 자동화된 고처리량 멀티플렉스 스크리닝 분석의 임상 평가Article Published on 2022-03-152022-09-11 Journal: Viruses [Category] SARS, 변종, 치료기술, 치료제, [키워드] amplification B.1.1.529 B.1.617.2 can be used Cell culture cell culture supernatant circulating clinical clinical samples Delta delta variant discrimination evaluated Healthcare system Healthcare systems high risk immunized large sample Lineage monoclonal antibody multiplex Multiplex assay NGS no interaction normalized omicron Omicron variant over P681H P681R patient treatment Population positive sample Primers probe probes public health measures qPCR RT-PCR RT-qPCR SARS-CoV-2 sensitivity sequence sequence variation Sequencing Spread target the SARS-CoV-2 Therapies variant Variant screening. variants of concern VoC VOCs was determined whole genome Whole genome sequencing [DOI] 10.3390/v14030608 PMC 바로가기 [Article Type] Article
Evidence of SARS-CoV-2 Related Coronaviruses Circulating in Sunda pangolins ( Manis javanica) Confiscated From the Illegal Wildlife Trade in Viet Nam베트남의 불법 야생 동물 거래에서 압수된 순다 천산갑(Manis javanica)에서 순환하는 SARS-CoV-2 관련 코로나바이러스의 증거Article Published on 2022-03-092022-09-11 Journal: Frontiers in Public Health [Category] SARS, 치료기술, [키워드] African Asia bats China Chinese pangolin Civet Confiscation coronavirus coronavirus Coronaviruses country curated Data collection detect downstream EID event evidence of Filovirus FIVE flavivirus Guangxi Hung Yen province investigated media one health orthomyxovirus PALM palm civet pangolin pangolins Paradoxurus paramyxovirus PCR assay performed Phylogenetic tree positive primer Primers raised RdRP RdRp gene reflected related virus RNA Sarbecoviruses SARS-CoV-2 SARS-related coronavirus SARS-related coronaviruses screened Seven specimen spillover Sunda pangolin Supply chain tested the Sarbecovirus Trade Trafficking viral family Viral RNA wildlife wildlife trade. zoonotic [DOI] 10.3389/fpubh.2022.826116 PMC 바로가기 [Article Type] Article
Optimization of the SARS-CoV-2 ARTIC Network V4 Primers and Whole Genome Sequencing ProtocolMedicine Published on 2022-02-172022-10-31 Journal: Frontiers in Medicine [Category] COVID-19, [키워드] Amplicon amplicon drop-offs amplified ARTIC Network ARTIC V4 average Beta characterized clinical sample Concentration correlation Delta delta variant Eta Genome genome coverage IMPROVE increase in instance optimization Oxford Nanopore Technology positive control positive controls primer Primers protocol released Result SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 sequencing SARS-CoV-2 variants sequenced statistically significant the SARS-CoV-2 V3 primer set variant Whole genome sequencing [DOI] 10.3389/fmed.2022.836728 PMC 바로가기 [Article Type] Medicine
Accuracy of Real-Time Polymerase Chain Reaction in COVID-19 PatientsCOVID-19 환자의 실시간 중합효소 연쇄 반응의 정확도Research Article Published on 2022-02-162022-09-10 Journal: Microbiology Spectrum [Category] COVID19(2023년), MERS, SARS, 치료기술, 치료법, 치료제, [키워드] E gene NP gene RdRp-gene Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addressed Analysis assays caused CDC CDC N1, N2 center chain Characteristics Clinical practice clinical samples conducted Control coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 patient cutoff value cycle threshold demonstrated Diagnosis diagnostic Diagnostic accuracy diagnostic specificity discrepancy disease control enrolled followed by Health Organization highest house lowest majority Mild nasopharyngeal nasopharyngeal sample Nasopharyngeal samples nucleocapsid protein Numerous Oropharyngeal oropharyngeal samples pandemic prevention primer primer set primer sets Primers Prospective Study qPCR qPCR analysis RdRP reaction Real-time PCR real-time polymerase chain reaction Research respiratory Respiratory illness RT-PCR RT-qPCR RT-qPCRs Saliva SARS-CoV-2 SARS-CoV2 sensitivity Sensitivity and specificity Seroconversion severe acute respiratory syndrome Coronavirus severe respiratory illness significantly specificity specimen specimens sputum Sputum sample Viral Viral detection WHO World Health Organization [DOI] 10.1128/spectrum.00591-21 PMC 바로가기 [Article Type] Research Article
Mass Screening of SARS-CoV-2 Variants using Sanger Sequencing Strategy in Hiroshima, Japan일본 히로시마에서 Sanger 시퀀싱 전략을 사용한 SARS-COV-2 변이체의 질량 스크리닝Article Published on 2022-02-142022-09-01 Journal: Scientific Reports [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] (Alpha) Alpha amplification amplified amplified product amplified products applied assigned B.1.1.529 B.1.1.7 B.1.351 B.1.617.1 B.1.617.2 Cluster confirmed case country COVID-19 develop E484K effective Effectiveness Epidemiology feasible genome sequence Genome sequences HCoV Hiroshima house identify indicate isolates Japan less mass Mass screening mutant Mutation next generation sequencing NGS notable number of sample number of samples P.1 pandemic PCR-based techniques positive Prevention and control primer primer set primer sets Primers protocol Randomly readiness reliability repeated reported RT-PCR Sanger sequencing SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants screened Screening screening strategy selected single mutation Strategy variant [DOI] 10.1038/s41598-022-04952-2 PMC 바로가기 [Article Type] Article
The Impact of Accumulated Mutations in SARS-CoV-2 Variants on the qPCR Detection EfficiencySARS-CoV-2 변이체의 누적 돌연변이가 qPCR 검출 효율에 미치는 영향Cellular and Infection Microbiology Published on 2022-01-282022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] SARS, 변종, 치료기술, [키워드] Delta delta variant demonstrated detection detection efficiency Detection limit Detection sensitivity Genome Impact less mutant Mutation N gene omicron Omicron variants primer Primers probe probes qPCR qPCR detection efficiency quantitative PCR Region SARS-CoV-2 SARS-CoV-2 variants sequence sequenced genome sequences several variant several variants variant variants VoC was reduced wt sequence [DOI] 10.3389/fcimb.2022.823306 PMC 바로가기 [Article Type] Cellular and Infection Microbiology