Defective Interfering Particles with Broad-Acting Antiviral Activity for Dengue, Zika, Yellow Fever, Respiratory Syncytial and SARS-CoV-2 Virus InfectionArticle Published on 2022-12-212023-07-10 Journal: Microbiology Spectrum [Category] COVID19(2023년), [키워드] antiviral therapy Chromatography coronavirus Defective Interfering Particle defective viral genome dengue virus purification respiratory syncytial virus RNA SARS-CoV-2 yellow fever virus Zika virus [DOI] 10.1128/spectrum.03949-22 PMC 바로가기
Sensitive detection of SARS-CoV-2 molecular markers in urban community sewersheds using automated viral RNA purification and digital droplet PCRArticle Published on 2022-11-152022-11-15 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 바이오마커, 치료기술, [키워드] affecting approach automated Care Community correlation COVID-19 COVID-19 case detection of marker Detroit disadvantage Effectiveness Epidemiology faster Final handling Health identify inhibitor Local marker molecular number of sample outbreak PCR PerkinElmer Prevent Promega public health authority purification required RNA RNA purification SARS-CoV-2 sensitive sensitivity Standard state the SARS-CoV-2 virus Thermofisher time-consuming Viral RNA Volume wastewater [DOI] 10.1016/j.scitotenv.2022.157547 PMC 바로가기
Protocol for high-throughput screening of SARS-CoV-2 main protease inhibitors using a robust fluorescence polarization assayArticle Published on 2022-10-032022-11-15 Journal: STAR protocols [Category] COVID19(2023년), SARS, 치료제, [키워드] antiviral agent Biophysics candidate Cell-based Assays Complete COVID-19 expression High Throughput Screening highest inhibitor MPro protease Protein Biochemistry Protein expression and purification protocol purification robust SARS-CoV-2 main protease virus [DOI] 10.1016/j.xpro.2022.101794 PMC 바로가기
Development and characterization of anti-SARS-CoV-2 intravenous immunoglobulin from COVID-19 convalescent plasmaArticle Published on 2022-10-012022-11-15 Journal: Immunotherapy [Category] COVID19(2023년), SARS, 진단, [키워드] administration Adults allergenic animal models anti-SARS-CoV-2 clinical trial Concentration convalescent plasma COVID-19 COVID-globulin development developmental Efficacy hamster healthy hyperimmune hyperimmune immunoglobulin IgG Immunoglobulin immunoglobulin against COVID-19 Immunosuppressed inactivation intravenous Intravenous immunoglobulin investigated material Neutralizing plasma preclinical evaluation produced Protective protective efficacy Protein purification Safe Safety SARS-CoV-2 SARS-CoV-2 neutralizing antibody Toxicity virus [DOI] 10.2217/imt-2022-0015 PMC 바로가기
Inactivation of SARS-CoV-2 and photocatalytic degradation by TiO2 photocatalyst coatingsArticle Published on 2022-09-262022-11-15 Journal: Scientific Reports [Category] SARS, 치료제, [키워드] acetaldehyde acute respiratory syndrome addition antiviral activity approach catalytic activity Contact coronavirus COVID-19 decrease Degradation effective Epidemic expand feature formaldehyde global pandemic inactivation Influenza virus investigated purification reached SARS-CoV-2 SEM significantly the epidemic transmitted virus [DOI] 10.1038/s41598-022-20459-2 PMC 바로가기
Baculovirus-Free SARS-CoV-2 Virus-like Particle Production in Insect Cells for Rapid Neutralization AssessmentArticle Published on 2022-09-202022-11-15 Journal: Viruses [Category] SARS, 진단, [키워드] ACE2 Analysis antibodies antibody approach assays assessment binding candidate Cell cellular cellular assay donors enzyme evaluate expression expression plasmid expression vector flow cytometric fluorescent fusion genomic information GFP insect insect cell insect cells labeling membrane membrane protein monoclonal antibody NTA Particle plasmid transfection Production purification quantification Rapid required SARS-CoV-2 SARS-CoV-2 envelope secondary antibody sera Spike protein TEM therapeutic transmission electron microscopy vaccinated individuals virus Virus-like particle Virus-like particles (VLPs) VLP VLPs [DOI] 10.3390/v14102087 PMC 바로가기
SARS-CoV-2 Production, Purification Methods and UV Inactivation for Proteomics and Structural StudiesArticle Published on 2022-09-082022-11-16 Journal: Viruses [Category] SARS, 유전자 메커니즘, [키워드] acute respiratory syndrome biosafety can be used cell activation conditions core Coronavirus-2 COVID-19 died downstream effective experiments faster handling inactivated inactivation Laboratory Microscopy pandemic pathogenicity Production purification resulting SARS-CoV-2 Sequencing Seven significantly size exclusion structural studies. sucrose cushion ultracentrifugation UV inactivation virus [DOI] 10.3390/v14091989 PMC 바로가기
Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffiiArticle Published on 2022-09-052022-11-16 Journal: Microbial Cell Factories [Category] SARS, 변종, [키워드] addition addressed aggregation Alpha Amino acid applied benefit caused Cell cleavage cleave demonstrated eliminated extension extracellular space functional Golgi highest Host IMPROVE Modification N-terminal N-terminus observation occur peptide Pichia Pichia pastoris product Product quality protease Protein protein engineering protein sequence Proteins purification Receptor binding domain Recombinant protein recombinant proteins residue Saccharomyces cerevisiae secrete secreted secreted protein secretion signal signal peptide steric steric hindrance subunit the SARS-CoV-2 the signal peptide therapeutic vaccine antigen variant variants [DOI] 10.1186/s12934-022-01905-2 PMC 바로가기
High-Throughput COVID-19 Testing of Naso-Oropharyngeal Swabs Using a Sensitive Extraction-Free Sample Preparation MethodArticle Published on 2022-08-312022-11-15 Journal: Microbiology Spectrum [Category] SARS, 치료기술, [키워드] approach calibrated Cobas costly COVID-19 detection system diagnosis of SARS-CoV-2 diagnostic Direct direct method downstream extraction-free extraction-free method gold standard High-throughput Infection International lack less log medium naso-oropharyngeal swab nasopharyngeal swab specimen nucleic acid extraction offer ORF1ab outcome PCR PCR assay per day PlexPCR pool positive sample preparation proteinase protocol purification quantification RdRP reagent reagents reduction in required Respiratory Coronavirus reverse transcription-PCR RNA Roche Sample SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 RNA sensitive sensitivity tested Testing time-consuming Transport Treatment Viral load Viral RNA WHO [DOI] 10.1128/spectrum.01358-22 PMC 바로가기
A platform technology for generating subunit vaccines against diverse viral pathogensArticle Published on 2022-08-182022-11-15 Journal: Frontiers in Immunology [Category] 진단, [키워드] acting affinity tag animal model Antigen binding can be used Clamp conformation COVID-19 pandemic Ebola virus elicit epitope fusion fusion protein glycoprotein immune response immunologically Infectious disease MERS-CoV Microscopy middle molecular monoclonal antibody mRNA neutralising Neutralising Antibodies Nipah virus offer platform precursor prefusion purification reagent remained respiratory syndrome coronavirus shown size exclusion chromatography stability stimulated Streamline subunit Subunit vaccine subunit vaccines tested trimeric Vaccine vaccine candidate vaccine platform Viral Viral antigen viral challenge viral pathogen virus [DOI] 10.3389/fimmu.2022.963023 PMC 바로가기