The Relationship Between Convergent IGH Signatures and Severity of COVID-19 Patients by Next-Generation Sequencing of B-Cell RepertoireMicrobiology Published on 2022-02-092022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] acute respiratory syndrome analyzed association B cell B-cell B-cell repertoire BCR clones coronavirus coronavirus disease COVID-19 Critical Deterioration FIVE Frequency groups healthy donor IGH Inpatient Mild motif next generation sequencing no significant difference object outcome Patient patients receptor recognize relationship repertoire Result SARS-CoV-2 severity signature significantly higher significantly lower three groups Treatment Vaccine development [DOI] 10.3389/fmicb.2021.833054 PMC 바로가기 [Article Type] Microbiology
T-Cell Repertoire Characteristics of Asymptomatic and Re-Detectable Positive COVID-19 Patients무증상 및 재검출 가능한 COVID-19 양성 환자의 T-세포 레퍼토리 특성Immunology Published on 2022-01-272022-09-11 Journal: Frontiers in Immunology [Category] 유전자 메커니즘, 치료기술, [키워드] analyzed Asymptomatic asymptomatic patient asymptomatic patients Combination convalescent Convalescent patients courses COVID-19 COVID-19 pandemic COVID-19 patient help immune repertoire sequencing immune response immune responses immunological Immunotherapy positive predict Prevalence re-detectable positive case recurrent infection repertoire risk Surveillance symptomatic T-cell receptor TCR [DOI] 10.3389/fimmu.2021.769442 PMC 바로가기 [Article Type] Immunology
Next-Generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire질량분석법에 의한 차세대 혈청학: SARS-CoV-2 항체 레퍼토리 판독Article Published on 2022-01-072022-09-11 Journal: Journal of Proteome Research [Category] SARS, 진단, 치료기술, [키워드] antibodies antibody Antibody detection Antigen applied Autoimmune disorders B cells capture captures Clonality clone COVID-19 data type degree heavy chains heterogeneity immune response immune responses Immunity immunized Immunoglobulin individual ion mass spectrometry Ion mass mass spectrometry Metrics Mild molecular pathogen Pathogens plasma Protein proteomics RBD Receptor-binding domain Region repertoire SARS-CoV-2 Sequencing serological serology Spike protein subject the receptor-binding domain the SARS-CoV-2 the spike protein titer top-down mass spectrometry. vaccination vaccine doses variable regions [DOI] 10.1021/acs.jproteome.1c00882 PMC 바로가기 [Article Type] Article
Analysis of TCR Repertoire by High-Throughput Sequencing Indicates the Feature of T Cell Immune Response after SARS-CoV-2 InfectionArticle Published on 2021-12-272022-10-31 Journal: Cells [Category] COVID-19, SARS, [키워드] analyzed caused CD4 CD8 CDR3 characteristic clearance clone Clustering Combination contributing to convalescent convalescent COVID-19 patients coronavirus Coronavirus disease 2019 COVID-19 COVID-19 patient COVID-19 patients dominant donors feature Frequency group healthy control immune memory immune response Infection Infectious disease Invasion J gene motif provide Recombination repertoire response SARS-CoV-2 SARS-CoV-2 antigen sequence Sequencing Specificities stages T cell T cell receptor T cell response TCR the healthy the SARS-CoV-2 virus virus recognition [DOI] 10.3390/cells11010068 PMC 바로가기 [Article Type] Article
Analysis of B Cell Receptor Repertoires Reveals Key Signatures of the Systemic B Cell Response after SARS-CoV-2 InfectionB 세포 수용체 레퍼토리 분석, SARS-CoV-2 감염 후 전신 B 세포 반응의 주요 특징 밝혀Vaccines and Antiviral Agents Published on 2021-12-082022-09-12 Journal: Journal of Virology [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] Activation Analysis analyzed antibody antibody repertoire Antibody Response Antibody titers B cell B cell receptor B cell response canonical Characteristics characterized clonal expansion clone Cluster collected correlated correlation COVID-19 patients differentiation elicited followed by germ line heavy chain highlighting IgA IgG IgG antibody IGHJ6 IGHV immune response Immunoglobulin individual isotype switching Lineage mAbs massive monoclonal antibodies monoclonal antibody mRNA mRNA transcript mRNA transcripts naïve B cells Neutralizing Peripheral blood peripheral blood samples proportion provided reactive repertoire reported response responses SARS-CoV-2 SARS-COV-2 infection sequence sequenced Sequencing serum antibody serum antibody response shared clonotype signature Somatic hypermutation spike-specific IgG sustained symptom onset systemic transcript transcriptional level Vaccines virus infection [DOI] 10.1128/jvi.01600-21 PMC 바로가기 [Article Type] Vaccines and Antiviral Agents
High-resolution epitope mapping and characterization of SARS-CoV-2 antibodies in large cohorts of subjects with COVID-19Article Published on 2021-11-222022-10-04 Journal: Communications Biology [Category] MERS, SARS, 바이오마커, 변종, 진단, [키워드] Adaptive immunity analyzed antibody epitopes Antibody Response Antigen applied approach Bacterial binding coronavirus 2 Coronaviruses COVID-19 cross-reactive D614G Data mining data-driven Diagnostic markers disease severity dominant epitope effective vaccine elicit epitope epitope mapping Evolution of SARS-CoV-2 highlight humoral identify immune response in silico analysis infected patient large cohort Mild motif mutant Mutant SARS-CoV-2 neutralization activity organism other coronavirus Patient peptide platform proteome proteomes random reduced Region repertoire respiratory SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2 epitope SARSCoV-2 serum samples severe disease specificity Spread Strains subject the vaccine Therapies therapy treat viral infection with COVID-19 [DOI] 10.1038/s42003-021-02835-2 PMC 바로가기 [Article Type] Article
TMPRSS2 promotes SARS-CoV-2 evasion from NCOA7-mediated restrictionTMPRSS2는 NCOA7 매개 제한으로부터 SARS-CoV-2 회피를 촉진합니다Research Article Published on 2021-11-222022-09-10 Journal: PLoS Pathogens [Category] MERS, SARS, 유전자 메커니즘, [키워드] acquisition Activation Antiviral Antiviral effect antiviral effects ATPase biological significance Cell cell surface characterized cleavage coactivator concomitant use consequence coronavirus Critical cytoplasmic dysregulation ectopic expression Effect effector endocytic Endosomal acidification epithelial cells evade furin furin cleavage furin cleavage site gene knockout HCoV HCoV-229E HCoV-NL63 highly pathogenic Host immune response host immune responses IFN IFN treatment IFNs immune responses Inborn errors Infection infections inhibit inhibit SARS-CoV-2 inhibited inhibitor innate immune response innate immune responses interact interferon interferons ISG ISGs isoform lentivirus less Lung epithelial cells lysosomal protease mechanism MERS MERS-CoV NCOA7 NL63 nuclear nuclear receptor option overexpression Particle pathway patients with COVID-19 plasma membrane polybasic promote protease Protein pseudotyped Pseudotyped viruses receptor removal repertoire Research RNA robust SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 entry SARS-COV-2 infection SARS-CoV-2 spike seasonal severe COVID-19 spike Spike protein Support the interferon TMPRSS TMPRSS2 TMPRSS2 protease Trigger vacuolar Vesicle Viral viral spike protein virus virus virus entry virus infection virus infections viruses [DOI] 10.1371/journal.ppat.1009820 PMC 바로가기 [Article Type] Research Article
Observed Antibody Space: A diverse database of cleaned, annotated, and translated unpaired and paired antibody sequencesTools for Protein Science Published on 2021-10-292022-10-28 Journal: Protein Science : A Publication of the Protein Soc [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome Amino acid annotated antibody sequences annotation antibody antibody database antibody repertoire antibody sequence available data B‐cell BCR‐seq coronavirus database disease driven by FASTQ files group increasingly individual nucleotide OAS Observed Observed Antibody Space (OAS) offer parameter problem provide receptor repertoire researcher sequence Sequencing sequencing data Space therapeutic translated vaccine responses Volume [DOI] 10.1002/pro.4205 PMC 바로가기 [Article Type] Tools for Protein Science
Specific CD8 + TCR Repertoire Recognizing Conserved Antigens of SARS-CoV-2 in Unexposed Population: A Prerequisite for Broad-Spectrum CD8 + T Cell ImmunityArticle Published on 2021-09-282022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] acute respiratory syndrome adaptive analyzed antibody immunity Antigen Blood broad-spectrum adaptive immunity CD8 CD8+ T cell CDR3 clone Clustering Cohort complementary conserved coronavirus coronavirus disease COVID-19 cross-reactive effort epitope Epitopes Evidence healthy healthy donor help Immunity implementation implicated IMPROVE in vitro individuals membrane memory T cell MHC-I mononuclear cell Mutation naïve neutralization nucleocapsid PBMCs peptide peptides peripheral populations proportion Protein Proteins provide recognizing recovered patient repertoire SARS-CoV-2 selected Sequencing Specific stimulated T cell T cell receptor T cells TCR TCR repertoire TCR sequencing the healthy therapy translational Vaccine variant virus [DOI] 10.3390/vaccines9101093 PMC 바로가기 [Article Type] Article
SCIGA: Software for large-scale, single-cell immunoglobulin repertoire analysisTechnical Note Published on 2021-09-282022-10-31 Journal: GigaScience [Category] COVID-19, SARS, [키워드] acute respiratory syndrome Analysis antibody B-cell Complete coronavirus coronavirus disease COVID-19 datasets finding help Immunoglobulin immunoglobulin repertoires lack neutralizing antibody Patient provide repertoire researcher SARS-CoV-2 sequence Sequencing single-cell Software statistical analysis was used [DOI] 10.1093/gigascience/giab050 PMC 바로가기 [Article Type] Technical Note