SARS‐CoV‐2 nucleocapsid protein interacts with immunoregulators and stress granules and phase separates to form liquid dropletsResearch Article Published on 2021-11-222022-10-31 Journal: Febs Letters [Category] COVID-19, [키워드] A549 A549 cell approach association biological processe blocked cancer cell Cancer cells cells droplet epithelial exhibited G3BP1 G3BP2 Heat shock human lung interact Interaction investigated liquid–liquid phase separation mitoxantrone N protein NCAP nucleocapsid nucleocapsid protein oxidative oxidative stress PKR Protein provide RNA RNA degradation RNase SARS‐CoV‐2 SARS‐CoV‐2 SILAC Stress stress granule stress granules translocated Treatment undergo USP10 viral factory viral infection YTHDF3 [DOI] 10.1002/1873-3468.14229 PMC 바로가기 [Article Type] Research Article
Intracellular RNase activity dampens zinc finger nuclease-mediated gene editing in hematopoietic stem and progenitor cellsResearch article Published on 2021-11-222022-10-05 Journal: Molecular Therapy [Category] 비임상, [키워드] Alter approach cells clinical trials complexes conditions CRISPR/Cas9 Culture dampen defined described disadvantage DNA ENhance example expression gene editing Gene therapy hematopoietic stem cells Host identify Intracellular mechanism media mRNA mRNA-based NHP Nonhuman primate models notable Nuclease over platform progenitor cell Protein ribonucleoprotein RNase RNase inhibitor tested therapeutic Zinc-finger nuclease [DOI] 10.1016/j.omtm.2021.11.010 [Article Type] Research article
SARS-CoV-2 infection triggers widespread host mRNA decay leading to an mRNA export blockArticle Published on 2021-11-012022-10-29 Journal: RNA (New York, N.Y.) [Category] COVID-19, MERS, SARS, [키워드] Antiviral antiviral response biogenesis caused cytoplasm cytoplasmic Degradation dissemination early stage elevated expression Genes hallmark Host host mRNA IFN immune response inhibit inhibited Innate immunity interferon IRF3 limit mammalian mRNA mRNA decay mRNA export mRNAs nucleus Patient performed Protein Regulatory RNA RNase RNase L SARS-CoV-2 SARS-CoV-2 infected cells SARS-COV-2 infection SARS-CoV-2 replication second serum severe COVID-19 disease Signaling the interferon the SARS-CoV-2 Transcription transcription factor transcriptional translocation Transport Trigger type I Type III IFN viral replication widespread [DOI] 10.1261/rna.078923.121 PMC 바로가기 [Article Type] Article
New targets for drug design: importance of nsp14/nsp10 complex formation for the 3′-5′ exoribonucleolytic activity on SARS-CoV-2약물 설계를 위한 새로운 표적: SARS-CoV-2에 대한 3'-5' 엑소리보핵분해 활성에 대한 nsp14/nsp10 복합체 형성의 중요성Article Published on 2021-09-012022-09-11 Journal: The Febs Journal [Category] SARS, 신약개발, 치료제, [키워드] activities activity Amino acid amino acids antagonist antiviral drugs assembly biochemical C-terminal catalytic complex Consequences conserved coronavirus Coronaviruses Critical DEDD disrupt domain ExoN extreme global pandemic implication in vitro in vitro activity independent independent of inhibitor inhibitors Interaction interfere interferon involved life cycle mapping molecular Molecular modeling motif MTase multifunctional protein N-terminal New nsp10 nsp14 other coronaviruses pathogenesis of SARS-CoV-2 performed Protein Replication required residue RNase SARS-CoV-2 SARS-CoV-2 virus stimulated target triggered upregulating virus [DOI] 10.1111/febs.15815 PMC 바로가기 [Article Type] Article
Reconstitution and functional characterization of SARS-CoV-2 proofreading complexSARS-CoV-2 교정복합체의 재구성 및 기능적 특성화Article Published on 2021-09-012022-09-11 Journal: Protein expression and purification [Category] SARS, 치료법, [키워드] activated acute respiratory syndrome coronavirus base pair base pairs characterized complex coronavirus 2 COVID-19 double-stranded RNA function functional functions in vitro inhibited metabolism mismatch nsp10 nsp14 NSP8 Nuclease pandemic primer Proofreading purified Replication replication of SARS-CoV-2 replication-transcription complex respiratory RNA RNA genome RNA replication RNAs RNase RTC SARS-CoV-2 SARS-CoV-2 replication SARS-CoV-2 RNA severe acute respiratory syndrome coronavirus 2 Structure structures the SARS-CoV-2 while [DOI] 10.1016/j.pep.2021.105894 PMC 바로가기 [Article Type] Article
Specificity and Mechanism of Coronavirus, Rotavirus, and Mammalian Two-Histidine Phosphoesterases That Antagonize Antiviral Innate ImmunityResearch Article Published on 2021-08-102022-10-29 Journal: mBio [Category] COVID-19, [키워드] 2-5A activate Activation activities AKAP7 Antiviral antiviral effects antiviral response cellular cleave cleaved coronavirus CoVs degrade enzyme Enzymes evolutionary relationship IFN Innate Innate immunity interferons investigated limit mammalian MERS-CoV MHV Middle East mouse CoV mouse hepatitis virus NS2 NS4b OAS phosphate producing Protein Regulation respiratory syndrome coronavirus RNA RNase RNase L rotavirus shown the interferon tissue damage trimer two-histidine phosphoesterase viral escape viral infection while [DOI] 10.1128/mBio.01781-21 PMC 바로가기 [Article Type] Research Article
Expression of Human ACE2 N-terminal Domain, Part of the Receptor for SARS-CoV-2, in Fusion With Maltose-Binding Protein, E. coli Ribonuclease I and Human RNase AMicrobiology Published on 2021-06-112022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] E. coli ribonuclease I (RNase I) ACE2 ACE2 protein Antiviral antiviral agent bacterial cell Cartoon cleaving degrade dsRNA E. coli enzyme evaluated expressed expression fusion fusion protein Genome Human human ACE2 receptor human RNase A-ACE2NTD fusion in vitro Inclusion Infection Membrane proteins N-terminal N-terminal domain Nucleocapsid proteins Part Protein Proteins provide purified receptor ribonuclease RNA RNA degradation RNase RNase I-ACE2NTD fusion RNase III RNases SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 viral SARS-CoV-2 virus single-stranded soluble fraction Strains viral genome viral nucleic acids virions [DOI] 10.3389/fmicb.2021.660149 PMC 바로가기 [Article Type] Microbiology
Rintatolimod Induces Antiviral Activities in Human Pancreatic Cancer Cells: Opening for an Anti-COVID-19 Opportunity in Cancer Patients?Article Published on 2021-06-092022-10-31 Journal: Cancers [Category] COVID-19, [키워드] activated activating activity adaptive immune system additional treatment option Anti-viral Antiviral effect block cancer patient cascade cells chemokine cytokine Cytokines death demonstrated epidemiological epithelial epithelial cancerous cells epithelial cells expression fibrosis Gene expression profiling Human independent induce infected with SARS-CoV-2 Infection innate immune system interferon signaling opening opportunity outcome overexpression pancreatic cancer cell pathway patients with COVID-19 Prevent producing proteomics Rintatolimod RNase RNase L SARS-CoV-2 SARS-COV-2 infection severity signaling pathway simple Specific Spread stimulated the disease the interferon TLR3 Toll-like receptor 3 treat Treatment treatment for COVID-19 treatment regimen uninfected USA virus replication was measured [DOI] 10.3390/cancers13122896 PMC 바로가기 [Article Type] Article
SARS-CoV-2 in hospital indoor environments is predominantly non-infectious병원 실내 환경에서 SARS-COV-2는 주로 비 영향을 미칩니다Research Published on 2021-06-022022-09-10 Journal: Virology Journal [Category] MERS, SARS, 치료기술, 치료법, [키워드] absence Complete Concentration COVID-19 Ct values degrade disease environmental sampling Future future work hospital Hospital environment in vitro In vitro infection infect Infection infectious Infectious disease Infectious virus investigated knowledge moderate non-infectious positive sample Prevent qPCR Result risk RNA RNA extraction RNase RNase A RT-qPCR SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 pandemic SARS-CoV-2 RNA SARS-CoV-2 virions SARS-CoV-2 virus Spread supplementary material the SARS-CoV-2 virus transmission route Transmission routes Treatment Uppsala University Vaccine Vero E6 Vero E6 cell VERO E6 cells Viral viral infectivity Viral RNA virus virus particle [DOI] 10.1186/s12985-021-01556-6 PMC 바로가기 [Article Type] Research
Comparison of SARS-CoV-2 indirect and direct RT-qPCR detection methodsSARS-CoV-2 간접 및 직접 RT-qPCR 검출 방법 비교Comparative Study Published on 2021-05-172022-09-01 Journal: Virology Journal [Category] SARS, 진단, 치료기술, [키워드] 2019-nCoV Analysis approach best BGI boiling caused Clinical diagnosis comparable Cost analysis COVID-19 COVID-19 pandemic Critical Ct values Detection limit detection system diagnostic diagnostics Direct detection evaluated exhibited extracted RNA gold gold standard handling initial Invitrogen isolation procedures less lysis buffer methodology MOST NEB other treatment Patient performed Primers provided purified viral RNA Qiagen reagents reduced Result RNA extraction RNA extraction kit RNA isolation RNase RNase inhibitor RT-qPCR SARS-CoV-2 SARS-CoV-2 virus sensitive sensitivity specificity supplementary material SYBR Green TaqMan tested the SARS-CoV-2 virus Universal Viral viral genome Viral RNA virus [DOI] 10.1186/s12985-021-01574-4 PMC 바로가기 [Article Type] Comparative Study