Development of Aptamers for RNase Inactivation in Xtract-Free™ Sample Collection and Transport MediumArticle Published on 2024-06-072024-09-05 Journal: Diagnostics [Category] update2024, [키워드] diagnostics DNA aptamers extraction-free detection home-collect nucleases point of care qPCR RNase sample collection Self-collect third generation specimen collection Xtract-Fee™ medium [DOI] 10.3390/diagnostics14121207 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Diverges from Other Betacoronaviruses in Only Partially Activating the IRE1α/XBP1 Endoplasmic Reticulum Stress Pathway in Human Lung-Derived CellsArticle Published on 2022-10-262022-11-15 Journal: mBio [Category] COVID19(2023년), SARS, 유전자 메커니즘, 진단, 치료기술, [키워드] activate Activation activities activity acute respiratory syndrome betacoronaviruses Cell cellular change coronavirus country Critical death downstream Endoplasmic reticulum enzyme ER stress examined expression of gene functions Gene Expression HCoV-OC43 Host host cell host cells host immune system Human human cell human cells imperative individual induce infect Infection inhibit interact IRE1α pathway less limit MERS-CoV MHV Middle East molecular mechanism mRNA murine OC43 Other other coronavirus pathway Prevent processed Replication required Respiratory Coronavirus respiratory syndrome coronavirus Reticulum RNase SARS-CoV SARS-CoV-2 SARS-COV-2 infection severe respiratory disease signaling pathway similarity splice Spread Stress tested the interferon transcription factor Trigger unfolded protein response UPR Vaccine viral replication virus XBP1 [DOI] 10.1128/mbio.02415-22 PMC 바로가기
Optimization of Anti-SARS-CoV-2 Treatments Based on Curcumin, Used Alone or Employed as a PhotosensitizerArticle Published on 2022-09-272022-11-15 Journal: Viruses [Category] SARS, 치료제, [키워드] anti-inflammatory properties Antiviral approach Cell culture cells Concentration COVID-19 Curcuma Curcumin dose effective immunomodulatory in vitro In vitro infection inhibited optimization photodynamic therapy photosensitizer prophylactic treatment reduction reported RNase SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 replication suggested therapy Treatment Vero E6 VERO E6 cells virion virus virus inactivation [DOI] 10.3390/v14102132 PMC 바로가기
SARS-CoV-2, SARS-CoV, and MERS-CoV encode circular RNAs of spliceosome-independent originSARS-CoV-2, SARS-CoV 및 MERS-CoV는 spliceosome-independent origin의 원형 RNA를 인코딩합니다.Article Published on 2022-07-012022-09-11 Journal: Journal of medical virology [Category] Coronavirus, COVID19(2023년), SARS, 유전자 메커니즘, 치료기술, 치료제, [키워드] abundance acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplified Analysis approaches assays Autoimmune disease Autoimmune diseases Betacoronavirus betacoronaviruses breakpoint canonical Chain Reaction characterized Circular circular RNA complementary coronavirus Critical cytoplasmic demonstrated distribution Features Fluorescence highlight homologous Host In situ hybridization infected Vero E6 cells junction mechanism MERS-CoV Middle East Middle East respiratory syndrome Coronavirus N gene not limited offer polymerase chain polymerase chain reaction predicted probe respiratory syndrome coronavirus RNA RNA Biology RNA genome RNA genomes RNA sample RNA samples RNA synthesis RNase SARS-CoV SARS-CoV-2 sequenced severe acute respiratory syndrome Coronavirus transcriptional regulatory sequences Transcriptome Vero E6 VERO E6 cells viral pathogenesis viral transcriptome Virology [DOI] 10.1002/jmv.27734 PMC 바로가기 [Article Type] Article
CASCADE: Naked eye-detection of SARS-CoV-2 using Cas13a and gold nanoparticlesCASCADE: Cas13a와 금 나노입자를 이용한 SARS-CoV-2 육안 탐지Article Published on 2022-05-012022-09-11 Journal: Analytica chimica acta [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, 치료법, 치료제, [키워드] acid activate aggregation approach approaches cascade cleave clinical sample clinical samples Concentration COVID-19 pandemic CRISPR CRISPR/Cas13 detect Diagnosis diagnostics Diseases effort gold Gold nanoparticle Gold nanoparticles Infectious diseases isothermal isothermal amplification molecular Naked-eye nanomaterials NASBA nasopharyngeal swabs nucleic acid nucleic acid amplification nucleic acids oligonucleotide Pathogens point-of-care Prevent Protein RNA RNase RPA SARS-CoV-2 SARS-CoV-2 RNA Scientific community sensitivity Spread ssRNA variety Viral viral nucleic acids Viral RNA [DOI] 10.1016/j.aca.2022.339749 PMC 바로가기 [Article Type] Article
Rational Design of CRISPR/Cas12a-RPA Based One-Pot COVID-19 Detection with Design of Experiments실험 계획을 통한 CRISPR/Cas12a-RPA 기반 One-Pot COVID-19 탐지의 합리적 설계Article Published on 2022-04-152022-09-11 Journal: ACS synthetic biology [Category] COVID19(2023년), SARS, 진단, 치료기술, [키워드] accelerate addition amplification applied approach benefit buffer complex component components condition COVID-19 COVID-19 pandemic CRISPR CRISPR/Cas12a critical impact definitive screening design demonstrated design of experiments detection Detection sensitivity diagnostic Diagnostic method diagnostics DNA DNA sequence DoE Effect effective experiment Factor full-length SARS-CoV-2 Genome highlight isothermal molecular Molecular diagnosis N gene one-pot COVID-19 testing optimization polymerase reaction reaction optimization Reactions recombinase polymerase amplification recombinase polymerase amplification (RPA). reverse transcription RNA RNase RNase inhibitor robust RPA RT-qPCR sensitivity significant effect significantly simple statistical variables [DOI] 10.1021/acssynbio.1c00617 PMC 바로가기 [Article Type] Article
Electrochemical biosensor for nucleic acid amplification-free and sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA via CRISPR/Cas13a trans-cleavage reactionCRISPR/Cas13a trans-cleavage 반응을 통한 중증 급성 호흡기 증후군 코로나바이러스 2(SARS-CoV-2) RNA의 핵산 증폭이 없고 민감한 검출을 위한 전기화학 바이오센서Article Published on 2022-04-012022-09-11 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), SARS, 치료기술, 치료법, [키워드] activated acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 artificial saliva biosensor cleave complex Concentration coronavirus COVID-19 COVID-19 pandemic CRISPR CRISPR/Cas13a detect diagnostic diagnostic testing dynamic range electrochemical biosensor Epidemic evaluated fatality Genes gold identify introduced nucleic acid occur ORF outbreak probe RNA RNase Saliva SARS-CoV-2 SARS-CoV-2 RNA severe acute respiratory syndrome Coronavirus ssRNA Trans-cleavage activity. Transmission Trigger virus detection [DOI] 10.1016/j.bios.2021.113960 PMC 바로가기 [Article Type] Article
Characterization of the SARS-CoV-2 ExoN (nsp14 ExoN –nsp10) complex: implications for its role in viral genome stability and inhibitor identificationAcademicSubjects/SCI00010 Published on 2022-02-222022-10-31 Journal: Nucleic Acids Research [Category] COVID-19, COVID19(2023년), SARS, [키워드] Antiviral assays blocked characterization classical complex coronavirus CoV COVID-19 Ebselen functional Genome genome replication global pandemic HIV identify implication in viral inhibit inhibitor M pro multifunctional non-structural protein nsp14 Proofreading protease RdRP Replication replication fidelity reveal RNA RNA genomes RNA structures RNA-dependent RNA polymerase RNase SARS-CoV-2 SARS-CoV-2 coronavirus stability the SARS-CoV-2 Treatment variety viral genome [DOI] 10.1093/nar/gkab1303 PMC 바로가기 [Article Type] AcademicSubjects/SCI00010
Photodynamic Inactivation of Human CoronavirusesArticle Published on 2022-01-082022-10-28 Journal: Viruses [Category] COVID-19, [키워드] absence Antiviral antiviral activity Antiviral effect antiviral property assays Betacoronavirus concentrations coronavirus CoVs cultured cell damaged demonstrated digestion dose-dependent manner doses emodin expand experiments HCoV-229E HCoV-OC43 herpes simplex Human human cells human coronavirus inactivation inhibited lentivirus Local microorganisms natural product oxygen PDI photodynamic inactivation photosensitizer PhytoQuin prevented Protein pseudotyped reactive oxygen specy Replication RNase ROS S protein SARS-CoV-2 spike susceptible the SARS-CoV-2 Viral RNA virus viruses [DOI] 10.3390/v14010110 PMC 바로가기 [Article Type] Article
Antigenic properties of the SARS-CoV-2 nucleoprotein are altered by the RNA admixtureBiotechnology Published on 2022-01-072022-10-28 Journal: PeerJ [Category] COVID-19, SARS, [키워드] absence Affect antibody Antigen antigenic caused complex cytoplasm Determining diagnostic E. coli ELISA Escherichia coli explained highest identify immunodominant IMPROVE in vitro investigated Isolation IVD N-terminal domain NaCl solution nucleic acid nucleoprotein presence of antibody purification resulting RNA RNase SARS-CoV-2 selective Sensitivity and specificity Serological testing serum sample significantly Stage subsequent symptoms of COVID-19 tested the SARS-CoV-2 Treatment Variation virus [DOI] 10.7717/peerj.12751 PMC 바로가기 [Article Type] Biotechnology