Modular micro-PCR system for the onsite rapid diagnosis of COVID-19 Research Published on 2022-07-012022-10-05 Journal: Microsystems & Nanoengineering [Category] 진단, 치료기술, [키워드] comparable COVID-19 COVID-19 pandemic detect diagnosis of COVID-19 diagnostic Endpoint gold standard isothermal amplification nasopharyngeal sample pathogen PCR platform point-of-care polymerase chain reaction provide reagents real-time RT-PCR reverse transcription RNA RNA extraction RNA extracts RT-LAMP RT-PCR RT-PCR assay SARS-CoV-2 SARS-CoV-2 virus [DOI] 10.1038/s41378-022-00400-3 [Article Type] Research
Diagnostic Accuracy of SARS-CoV-2 Nucleocapsid Antigen Self-Test in Comparison to Reverse Transcriptase-Polymerase Chain ReactionArticle Published on 2022-06-302022-11-15 Journal: The journal of applied laboratory medicine [Category] COVID19(2023년), SARS, 진단, [키워드] Accuracy Antigen approved chain colloidal gold conducted conserved Council COVID-19 COVID-19 diagnosis diagnostic Effects evaluated false-negative ICMR Limited Mutation N-antigen nucleocapsid antigen offered ORF1ab gene positive result rapid test kit reaction RT-PCR RT-PCR assay RT-PCR Ct RT-PCR kit RT–PCR S-gene SARS-CoV-2 self-test. sensitivity stability the disease the patient Thermo Fisher Scientific threshold validation study was performed [DOI] 10.1093/jalm/jfac023 PMC 바로가기
Anti-inflammatory response of cardamom extract and prediction of therapeutic window in COVID-19 patients by assessing inflammatory markers using RT-PCRRT-PCR을 사용한 염증 마커를 평가함으로써 COVID-19 환자에서 카 다몬 추출물의 항 염증 반응 및 치료 창의 예측Article Published on 2022-06-012022-08-31 Journal: Inflammopharmacology [Category] COVID19(2023년), MERS, SARS, 진단, 치료제, [키워드] addition alternative Anti-inflammatory anti-inflammatory action anti-inflammatory response Anti-viral association attending physician Awareness breathlessness can not Cardamom Clinical improvement Clinical practice clinician control group cough Course COVID-19 COVID-19 patient COVID-19 patients COVID-19 symptom COVID-19 symptoms CRP Ct values cycle threshold cytokine Cytokine storm dawn Diagnostic method diagnostic test diagnostic tests disease Disease progression dosage dose effective elevated end point enrolled Fever FORTE gene expression profile hospital IL-10 IL-6 India indicate Inflammation Inflammatory marker inflammatory markers inflammatory parameter intake Intervention lack LDH majority material molecular diagnostics morbidity morbidity and mortality multiplex negativity normalized onset of symptom outcome parameter Patient patients PCR test performed popularity potentiality predict Prednisolone prevalent Prognosis protein level provide reason for reducing reported RT-PCR RT-PCR analysis RT-PCR assay RT-PCR test SARS-CoV-2 SARS-CoV-2 RT-PCR SARS-CoV-2 RT-PCR test serum Serum IL-6 sore throat Standard of care standard treatment Steroids Symptom Tablet tested the cytokine storm the patient therapeutic window time TNF- α Treatment treatment arm treatment group were given worsening [DOI] 10.1007/s10787-022-00951-x PMC 바로가기 [Article Type] Article
A screening strategy for identifying the dominant variant of SARS-COV-2 in the fifth peak of Kurdistan- Iran population using HRM and Probe-based RT-PCR assayHRM 및 프로브 기반 RT-PCR 분석을 사용하여 쿠르드-이란 인구의 다섯 번째 피크에서 SARS-COV-2의 우세한 변이를 식별하기 위한 스크리닝 전략Article Published on 2022-06-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), SARS, 변종, [키워드] caused country D614G D614G mutation Del69-70 mutation diagnostic test dominant variant E484K E484K mutation E484Q mutation genomic Genomic surveillance high resolution HRM identify indicated Iran Kurdistan L452R L452R mutation melting mortality rate Mutation operation overlapped positive patient probe Probe-based RT-PCR reduce Resolution RT-PCR RT-PCR assay SARS CoV SARS CoV-2 SARS-CoV-2 positive patients screened screening strategy Sequencing suggested Surveillance T478K T478K mutation. Transmissibility variant variants whole-genome sequencing [DOI] 10.1016/j.jviromet.2022.114514 PMC 바로가기 [Article Type] Article
Semi-nested RT-PCR enables sensitive and high-throughput detection of SARS-CoV-2 based on melting analysisSemi-nested RT-PCR은 용융 분석을 기반으로 SARS-CoV-2의 민감하고 높은 처리량 검출을 가능하게 합니다.Article Published on 2022-06-012022-09-11 Journal: Clinica chimica acta; international journal of cli [Category] COVID19(2023년), SARS, 치료기술, [키워드] 95% CI 96-well plate Achille Algorithm Amplicon Analysis artificial artificial intelligence Artificial intelligent clinical collected complex COVID-19 COVID-19 pandemic evaluated Factors High-throughput PCR implementation In-vitro indicated melting Melting analysis molecular multiplex Nasopharyngeal samples PCR pooling provide reagent Real-time PCR RNA RNAs RT-PCR RT-PCR assay SARS-CoV-2 SARS-CoV-2 RNA screened screening strategy Semi Semi-nested. sensitivity solution specificity Support Transmission [DOI] 10.1016/j.cca.2022.04.997 PMC 바로가기 [Article Type] Article
Evaluating the performance characteristics of five lateral flow assays for the detection of the SARS-CoV-2 nucleocapsid antigenSARS-CoV-2 뉴클레오캡시드 항원 검출을 위한 5가지 측면 유동 분석의 성능 특성 평가Article Published on 2022-05-252022-09-11 Journal: Scientific Reports [Category] COVID19(2023년), SARS, 진단, 치료기술, 치료법, [키워드] 95% CI Accuracy acquisition age assays caution Chain Reaction Characteristics China collected Community complement complementary costly COVID-19 COVID-19 negative COVID-19 pandemic diagnostic Diagnostic accuracy early diagnosis ENCODE Evaluating facilitate female FIVE hospitalised individuals with COVID-19 Infection Inpatient Italy lateral flow MHRA nucleocapsid antigen outcomes Oxygenation Participants Patient patients with COVID-19 PCR testing performed polymerase chain polymerase chain reaction protocol ranged reverse-transcriptase polymerase chain reaction Rome RT-PCR RT-PCR assay RT-PCR assays RT-PCR testing SARS-CoV-2 SARS-CoV-2 antigen sensitivity swabbing the SARS-CoV-2 thresholds variable Variation was performed WHO [DOI] 10.1038/s41598-022-12805-1 PMC 바로가기 [Article Type] Article
Combined kinetic analysis of SARS-CoV-2 RNAemia, N-antigenemia and virus-specific antibodies in critically ill adult COVID-19 patients중증 성인 COVID-19 환자의 SARS-CoV-2 RNA혈증, N-항원혈증 및 바이러스 특이 항체의 결합 운동 분석Article Published on 2022-05-182022-09-11 Journal: Scientific Reports [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, 치료법, [키워드] adult COVID-19 patient analyses Analysis antibody Antibody detection antigenemia bloodstream CAST Cohort collected Combined component COVID-19 patient COVID-19 patients Critically ill decreasing detection rate dissemination examined Free help immunoassays immunochromatography inverse correlation kinetic analysis lateral flow median median age median time nucleocapsid onset of symptoms patients performed plasma RBD Receptor binding domain respiratory RNA RT-PCR assay SARS-CoV-2 SARS-CoV-2-RBD specific antibodies specimen symptoms onset upper respiratory tract Viral Viral load virus virus clearance virus-specific antibodies [DOI] 10.1038/s41598-022-12461-5 PMC 바로가기 [Article Type] Article
Establishment of an in-house real-time RT-PCR assay for the detection of severe acute respiratory syndrome coronavirus 2 using the first World Health Organization international standard in a resource-limited country자원이 제한된 국가에서 최초의 세계보건기구 국제표준을 이용한 중증급성호흡기증후군 코로나바이러스 2 검출을 위한 사내 실시간 RT-PCR 분석법 구축Article Published on 2022-05-012022-09-11 Journal: Journal of Clinical Laboratory Analysis [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome caused Clinical performance clinical sample clinical samples conserved coronavirus correlation country COVID-19 COVID-19 pandemic Critical E gene evaluated house International international standard limit of detection molecular molecular assays multiplex N gene Nasopharyngeal samples Nasopharyngeal swab nasopharyngeal swabs NIBSC Patient PCR assay positive Primers probe probes public health real-time RT-PCR reference standard Region RT-PCR assay Saliva SARS-CoV-2 specimen Standard Target genes WHO World Health Organization [DOI] 10.1002/jcla.24355 PMC 바로가기 [Article Type] Article
SARS-CoV-2 circulation in Croatian wastewaters and the absence of SARS-CoV-2 in bivalve molluscan shellfish크로아티아 폐수에서 SARS-CoV-2 순환 및 이매패류 연체동물 조개에서 SARS-CoV-2 부재Article Published on 2022-05-012022-09-11 Journal: Environmental research [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] absence amplification Bioaccumulation Bivalve molluscan shellfish BMS circulation collected Community Concentration Contamination criterion Croatia eight Epidemic epidemic wave food safety knowledge mengovirus nine nsp14 plant processed Protein provided real-time RT-PCR Research risk rotavirus Rotavirus A RT-PCR assay SARS-CoV-2 SARS-CoV-2 detection Sequencing shellfish surface Surface water Surface waters Treatment virus warty was performed Wastewaters. water [DOI] 10.1016/j.envres.2021.112638 PMC 바로가기 [Article Type] Article
Factors associated with cycle threshold values (Ct-values) of SARS-CoV2-rRT-PCRSARS-CoV 2-rT-PCR의 주기 역치 값(Ct 값)과 관련된 요인Article Published on 2022-05-012022-09-11 Journal: Molecular biology reports [Category] COVID19(2023년), SARS, 치료기술, [키워드] age association Care caution clinical clinical history COVID-19 COVID-19 severity COVID-19 testing Ct values Ct-values cycle cycle threshold Cycle threshold value Cycle threshold values demographics diagnostic distribution distributions examined Factor Health Health care individual information limitation N gene ORF1ab Patient PCR assays PCR test RdRP RdRp gene RdRp genes reported RT-PCR assay RT-PCR assays RT-PCR test SARS-CoV-2 SARS-CoV-2 positive patient SARS-CoV-2 positive patients SARS-CoV-2 RT-PCR test Sex Symptom Symptomatology the patient the SARS-CoV-2 threshold was collected [DOI] 10.1007/s11033-022-07360-x PMC 바로가기 [Article Type] Article