Identification of the immunodominant neutralizing regions in the spike glycoprotein of porcine deltacoronavirusArticle Published on 2019-12-062022-11-01 Journal: Virus research [Category] Coronavirus, MERS, [키워드] Amino acid Antibody Response Antisera C-terminal domain coronavirus CTD defined E. coli effective effective therapy Epitope region expression fluorescent Host identification immunodominant in vitro in vivo Infection inhibit mice N-terminal domain neutralization Neutralization assay Neutralizing Neutralizing antibodies neutralizing antibody NTD Porcine deltacoronavirus porcine deltacoronavirus (PDCoV) produced Protein Recombinant protein Region S protein S1 subunit S2 subunit spike glycoprotein the S protein the S1 subunit Transmission Vaccine Vaccines Virus neutralization were expressed [DOI] 10.1016/j.virusres.2019.197834 PMC 바로가기 [Article Type] Article
An Optimized High-Throughput Neutralization Assay for Hepatitis E Virus (HEV) Involving Detection of Secreted Porf2Communication Published on 2019-01-152023-05-24 Journal: Viruses [Category] E형 간염, Fulltext, [키워드] hepatitis E virus high throughput Neutralization assay secreted pORF2 [DOI] 10.3390/v11010064 PMC 바로가기 [Article Type] Communication
Host-Pathogen Coevolution and the Emergence of Broadly Neutralizing Antibodies in Chronic Infections숙주-병원체 공진화와 만성 감염에서 광범위하게 중화되는 항체의 출현Research Article Published on 2016-07-212022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] adaptation adaptive immune system antibody Antigen antigens arms race binding affinities binding interaction binding interactions cellular changes in chronic chronic infection chronic infections coevolution competition condition Critical determine develop distribution driven by emergence Evolution evolutionary arms race external environment favor flexible focus Frequency Genotype HIV HIV-infected patients Host identify immune immune parameter immune population immune response infections Interaction Lineage mathematical measure molecules Mutation Neutralization assay neutralization assays neutralize Neutralizing antibodies neutralizing antibody occur Organisms parameter Pathogens Population populations provide Rapid resulting Vaccine design Viral virus viruses [DOI] 10.1371/journal.pgen.1006171 PMC 바로가기 [Article Type] Research Article
Isolation and Characterization of Dromedary Camel Coronavirus UAE-HKU23 from Dromedaries of the Middle East: Minimal Serological Cross-Reactivity between MERS Coronavirus and Dromedary Camel Coronavirus UAE-HKU23Article Published on 2016-05-072022-10-29 Journal: International Journal of Molecular Sciences [Category] Coronavirus, [키워드] Analysis Antibody assays antibody tests Antibody titer Camel cells characterization Cluster codon coronavirus correlation CoV Cytopathic effect Dromedary Dromedary camel Fecal sample FIVE Genetic heat-inactivated immunofluorescence Isolation MERS MERS-CoV mice middle Middle East motif N gene N protein neutralization Neutralization assay Northern blot analysis Pearson predicted RdRP reported respiratory syndrome coronavirus RNA separated sequence sera Seven strain subgenomic mRNA transcription regulatory sequence [DOI] 10.3390/ijms17050691 PMC 바로가기 [Article Type] Article
Development of an indirect ELISA, blocking ELISA, fluorescent microsphere immunoassay and fluorescent focus neutralization assay for serologic evaluation of exposure to North American strains of Porcine Epidemic Diarrhea VirusMethodology Article Published on 2015-08-012022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] addition Analysis antibody Asia asked assays biotinylated calculated colostrum Coronaviruses correlation country cross-reactivity demonstrated described develop development diagnostic test Disadvantages ELISA epidemic diarrhea expressed fluorescent Fluorescent Focus Neutralization (FFN) Fluorescent microsphere immunoassay (FMIA) functional gastroenteritis highlight IFA Immune status immunoassay immunohistochemistry Interpretation kappa value Laboratory mAb mAbs magnetic bead measurement microsphere MONITOR monoclonal monoclonal antibody multiple antigen naïve Neutralization assay Neutralizing antibodies neutralizing antibody Neutralizing antibody response North North America outbreak pathogen PEDV porcine epidemic diarrhea virus (PEDV) protective immunity quantitation question receiver operating characteristic recent Respiratory Coronavirus Result ROC ROC analysis Sensitivity and specificity Seroconversion Serological assay serology serum serum sample significant level Spread staining strain TGEV vaccine candidate virus virus neutralizing antibodies was performed were used [DOI] 10.1186/s12917-015-0500-z PMC 바로가기 [Article Type] Methodology Article
An inactivated vaccine made from a U.S. field isolate of porcine epidemic disease virus is immunogenic in pigs as demonstrated by a dose-titrationResearch Article Published on 2015-03-152022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] Administered caused Cell culture Complete Culture demonstrated diarrhea disease dose Efficacy ELISA enzyme-linked immunosorbent Epidemic epidemic diarrhea evaluate experimental design FIVE fluorescent foci Genome sequencing help highly pathogenic humoral immune response identity immunoassay immunogenic immunogenicity inactivated Inactivated vaccine infective intramuscularly log microsphere monovalent vaccine negative controls Neutralization assay nucleotide PEDV performed pig Porcine Protective responses Result selected sera significantly higher strain TCID tissue vaccinate Vaccine vaccine candidate virus [DOI] 10.1186/s12917-015-0357-1 PMC 바로가기 [Article Type] Research Article
Serologic Status for Pandemic (H1N1) 2009 Virus, TaiwanDispatch Published on 2011-01-012023-06-08 Journal: Emerging Infectious Diseases [Category] Fulltext, 신종인플루엔자, [키워드] dispatch Hemagglutination inhibition assay Influenza Neutralization assay Pandemic (H1N1) 2009 serologic assays Taiwan Vaccine viruses [DOI] 10.3201/eid1701.100014 PMC 바로가기 [Article Type] Dispatch