Genetic and antigenic characterization of recombinant nucleocapsid proteins derived from canine coronavirus and canine respiratory coronavirus in ChinaResearch Paper Published on 2016-04-152022-10-28 Journal: Science China. Life sciences [Category] Coronavirus, [키워드] Analysis antigenic antigenicity Antisera Beijing canine Canine coronavirus canine respiratory coronavirus China cloned conserved coronavirus Coronavirus infection cross-react cross-reactivity E. coli Escherichia coli expressed expression Final group HCoV-229E HKU1 human coronaviruses indicated mice N gene NL63 nucleocapsid protein OC43 polyacrylamide gel purified raised Respiratory Coronavirus SDS-PAGE sequenced size exclusion chromatography sodium dodecyl sulfate Swab virus were expressed [DOI] 10.1007/s11427-016-5038-1 PMC 바로가기 [Article Type] Research Paper
Development of an indirect ELISA, blocking ELISA, fluorescent microsphere immunoassay and fluorescent focus neutralization assay for serologic evaluation of exposure to North American strains of Porcine Epidemic Diarrhea VirusMethodology Article Published on 2015-08-012022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] addition Analysis antibody Asia asked assays biotinylated calculated colostrum Coronaviruses correlation country cross-reactivity demonstrated described develop development diagnostic test Disadvantages ELISA epidemic diarrhea expressed fluorescent Fluorescent Focus Neutralization (FFN) Fluorescent microsphere immunoassay (FMIA) functional gastroenteritis highlight IFA Immune status immunoassay immunohistochemistry Interpretation kappa value Laboratory mAb mAbs magnetic bead measurement microsphere MONITOR monoclonal monoclonal antibody multiple antigen naïve Neutralization assay Neutralizing antibodies neutralizing antibody Neutralizing antibody response North North America outbreak pathogen PEDV porcine epidemic diarrhea virus (PEDV) protective immunity quantitation question receiver operating characteristic recent Respiratory Coronavirus Result ROC ROC analysis Sensitivity and specificity Seroconversion Serological assay serology serum serum sample significant level Spread staining strain TGEV vaccine candidate virus virus neutralizing antibodies was performed were used [DOI] 10.1186/s12917-015-0500-z PMC 바로가기 [Article Type] Methodology Article
Development and Application of an ELISA for the Detection of Porcine Deltacoronavirus IgG AntibodiesResearch Article Published on 2015-04-162022-10-28 Journal: PLoS ONE [Category] Coronavirus, MERS, [키워드] addition antibody application collected coronavirus cross-reactivity detect detection determine development diagnostic disease ELISA enzyme-linked immunosorbent epidemic diarrhea gastroenteritis IgG IgG antibody Infection introduced North America nucleic acid other coronaviruses PEDV Prevalence Respiratory Coronavirus resulting selected serum sample specificity tested The diagnostic sensitivity the spike protein virus while [DOI] 10.1371/journal.pone.0124363 PMC 바로가기 [Article Type] Research Article
T cell-mediated immune response to respiratory coronavirusesArticle Published on 2014-05-212023-06-16 Journal: Immunologic research [Category] MERS, [키워드] immune response Respiratory Coronavirus T cells [DOI] 10.1007/s12026-014-8534-z PMC 바로가기
Yeast Based Small Molecule Screen for Inhibitors of SARS-CoVResearch Article Published on 2011-12-022022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] activities activity Alter bypasse Cell culture cells cellular connection conserved Course demonstrated deubiquitinase Diversity expressed functional growth human coronaviruses induce induction Influenza virus inhibit inhibited inhibiting the function inhibitor inhibitory compounds investigated likelihood mammalian cells mechanism modify molecule Mortality NIH outbreak Papain-like protease phenotype PLP precursor protease Protease activity Replication reservoir Respiratory Coronavirus resulting S. cerevisiae SARS-CoV Screen severe shown small molecule suppressor toxic effect virus replication yeast [DOI] 10.1371/journal.pone.0028479 PMC 바로가기 [Article Type] Research Article
Molecular characterization of a canine respiratory coronavirus strain detected in Italy ☆Article Published on 2009-01-202022-11-01 Journal: Virus research [Category] Coronavirus, MERS, [키워드] Accessory genes bovine Bovine coronavirus canine canine respiratory coronavirus CoVs described detected different disease ENCODE envelope protein feature Gene Genetic Genomic 3′-end genomic region Genotype host tropism humans Italian Italy mutant non-structural protein Point mutation Protein Recombination related Respiratory Coronavirus responsible RNA virus sequence Sequence analysis structural protein suggested tissue unique Viral RNA virus [DOI] 10.1016/j.virusres.2008.12.011 PMC 바로가기 [Article Type] Article
Human respiratory coronavirus HKU1 versus other coronavirus infections in Italian hospitalised patientsArticle Published on 2007-01-102022-11-01 Journal: Journal of Clinical Virology [Category] Coronavirus, [키워드] 229E Acute respiratory tract infections Analysis ARTI, acute respiratory tract infections BAL, bronchoalveolar lavage caused clinical symptom compared contribute Coronavirus infection detected DFA, direct fluorescent antibody staining Diagnosis diagnostic epidemiological feature found Genotype HCoV HCoVs hCoVs, human coronavirus HKU1 HKU1 genotypes Hong Kong hospitalised hospitalised patient Human Human coronavirus HKU1 identify Infected Infection infections Italian Italy longitudinal MAbs, monoclonal antibodies monoclonal antibody nine NPA, nasopharyngeal aspirate objective Patient patient population patients performed predominant Prospective Study reported Respiratory Coronavirus respiratory tract infection Result reverse transcription-PCR rhinorrhea RT-PCR RT-PCR, reverse transcription-PCR significantly lower strain SVC, shell-vial cultures [DOI] 10.1016/j.jcv.2006.12.008 PMC 바로가기 [Article Type] Article