Structural Analysis of the Novel Variants of SARS-CoV-2 and Forecasting in North AmericaSARS-CoV-2의 새로운 변종 구조 분석 및 북미 예측Article Published on 2021-05-172022-09-01 Journal: Viruses [Category] SARS, 변종, 진단, [키워드] angiotensin B.1.1.7 B.1.1.7 variant B.1.351 B.1.617 circulating computational modeling COVID-19 database dose enzyme Forecasting hACE2 herd immunity high affinity human Angiotensin-converting enzyme Human angiotensin-converting enzyme 2 Immunity Interaction Isolation less Mutation Neutralizing antibodies neutralizing antibody neutralizing immunity Nextstrain North America novel P.1 variant P.1 variants Patient predicted prevalent proportion reached receptor reduce S-RBD SARS-CoV-2 spike receptor-binding domain transmission rates Vaccine variant variants Viral viral mutation Viral mutations wild-type [DOI] 10.3390/v13050930 PMC 바로가기 [Article Type] Article
A bivalent recombinant vaccine targeting the S1 protein induces neutralizing antibodies against both SARS‐CoV‐2 variants and wild‐type of the virusOriginal Article Published on 2021-05-172022-10-30 Journal: MedComm [Category] COVID-19, [키워드] ACE2 binding acute respiratory syndrome antibody production Antigen AS03 B.1.351 variant bivalent vaccine Clinical use composed COVID‐19 D614G E484K effective enzyme exhibited Express hACE2 immunization immunized induce inhibiting K417N mice Mutant strains N501Y neutralization neutralizing antibody other variants pandemic protein subunit vaccine receptor‐binding domain Recombinant protein S1 subunit SARS‐CoV‐2 SARS‐CoV‐2 subunit the S1 protein transgenic mice Vaccine variant virus Virus Disease were measured [DOI] 10.1002/mco2.72 PMC 바로가기 [Article Type] Original Article
Human Surfactant Protein D Binds Spike Protein and Acts as an Entry Inhibitor of SARS-CoV-2 Pseudotyped Viral ParticlesImmunology Published on 2021-05-142022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, SARS, [키워드] 16HBE Act acute respiratory syndrome angiotensin converting enzyme 2 animal models bind binding cells composed coronavirus CRD enzyme epithelial Ex vivo expressing hACE2 HCoV-229E HEK293T cell highlight Human human pulmonary collectins immune in vitro in vivo influenza A inhibited inhibitor innate immune Innate immunity Interaction mucosal surface overexpressing Particle Pathogens previous study protective role pseudotyped lentiviral particle Receptor binding domain reduction in reported respiratory RLU S1 protein SARS-CoV SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 S1 serum spike spike glycoprotein Spike protein suggested surfactant Surfactant protein D Surveillance the patient therapeutic potential Treatment viral entry viral pathogen [DOI] 10.3389/fimmu.2021.641360 PMC 바로가기 [Article Type] Immunology
The interferon-stimulated exosomal hACE2 potently inhibits SARS-CoV-2 replication through competitively blocking the virus entryArticle Published on 2021-05-122022-10-04 Journal: Signal Transduction and Targeted Therapy [Category] Fulltext, SARS, 치료제, [키워드] acute respiratory syndrome Antiviral cell entry cell types coronavirus coronavirus disease COVID-19 Ex vivo exhibiting Exosome expression global pandemic hACE2 human Angiotensin-converting enzyme IFN in vitro indicated inhibit Innate immunity interferon Microbiology outbreak Protein receptor recognize released replication of SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 replication Treatment virus entry [DOI] 10.1038/s41392-021-00604-5 PMC 바로가기 [Article Type] Article
In Silico Studies of Some Isoflavonoids as Potential Candidates against COVID-19 Targeting Human ACE2 (hACE2) and Viral Main Protease (M pro )Article Published on 2021-05-102022-10-30 Journal: Molecules [Category] COVID-19, [키워드] ACE2 acute respiratory syndrome affinity Anti-SARS-CoV-2 Activity antiviral activity binding energy binding mode candidate carried caused China co-crystallized Compound coronavirus COVID-19 disease docking result drug-likeness examined hACE2 Human human ACE2 in silico Interaction Isoflavonoid isoflavonoids Ligand M pro main protease molecular docking Molecular docking study pandemic Physicochemical properties Potential reported SARS-CoV-2 specific treatment targeting Toxicity unique WHO widespread [DOI] 10.3390/molecules26092806 PMC 바로가기 [Article Type] Article
Human convalescent plasma protects K18-hACE2 mice against severe respiratory disease인간 회복기 혈장은 심각한 호흡기 질환으로부터 K18-hACE2 마우스를 보호합니다Animal Published on 2021-05-072022-09-12 Journal: The Journal of General Virology [Category] SARS, 진단, [키워드] angiotensin animal model animal models anti-SARS-CoV-2 antibody antibody protection Brain Brain infection brains candidate drug candidate drugs causative agent convalescent plasma COVID-19 determine develop disease Efficacy element expressing hACE2 health emergency Host Human human Angiotensin-converting enzyme human infection human infections hypersensitivity infect Infection intranasal K18 K18-hACE2 K18-hACE2 mice K18-hACE2 mouse model Keratin lesion lesions lung Lungs medical countermeasure mice mitigate Neutralizing antibodies persistence Post-infection Prevent promoter PROTECT Protective pulmonary damage receptor reduced reported Respiratory disease SARS-CoV-2 severe disease severe respiratory disease Spread subsequent Support survived Therapeutics this system transgenic mice treated Viral antigen virus [DOI] 10.1099/jgv.0.001599 PMC 바로가기 [Article Type] Animal
Structural basis for SARS-CoV-2 neutralizing antibodies with novel binding epitopes신규 결합 에피토프를 갖는 SARS-CoV-2 중화 항체의 구조적 기초Research Article Published on 2021-05-072022-08-13 Journal: PLoS Biology [Category] Biochemistry, SARS, 진단, [키워드] ACE2 receptor acute respiratory syndrome coronavirus Ad5-hACE2 Ad5-hACE2 transduction mouse model angiotensin antibodies antibody antiviral efficiency applied binding binding epitope caused characterized coronavirus coronavirus 2 coronavirus disease Coronavirus disease 2019 COVID-19 domain Economy epitope Epitopes global public health H2L2 platform H2L2 transgenic mice hACE2 help High-resolution X-ray crystallography structures human Angiotensin-converting enzyme Human angiotensin-converting enzyme 2 human immunoglobulin human immunoglobulin variable regions humanized NAb identify immunize Immunoglobulin injection mAb mAb-related therapeutic intervention mAb-related therapeutic interventions monoclonal antibody mouse model NAbs Neutralizing activity Neutralizing antibodies neutralizing antibody novel non-blocking epitope pandemic platform PR1077 PR953 PR961 Prophylactic provide provided RBD RBM receptor-binding motif recombinant vaccines regions respiratory SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 neutralizing antibody SARS-CoV-2 spike protein Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Spike protein spike receptor-binding domain Structural analysis Structure subsequent tested the antibody the SARS-CoV-2 the spike protein therapeutic interventions therapy transgenic mice treatment groups variable regions X-ray crystallography [DOI] 10.1371/journal.pbio.3001209 PMC 바로가기 [Article Type] Research Article
Glycated ACE2 receptor in diabetes: open door for SARS-COV-2 entry in cardiomyocyteOriginal Investigation Published on 2021-05-072022-10-31 Journal: Cardiovascular Diabetology [Category] COVID-19, SARS, [키워드] ACE2 ACE2 expression ACE2 protein ACE2 receptor affecting alterations Autopsies Autopsy binding cardiomyocyte cellular entry coronavirus disease COVID-19 COVID-19 infection Deceased diabete diabetes diagnosed domain Effect evaluate evaluate the effect evaluated expression Glucose glycosylated glycosylated forms hACE2 heart Hospitalized human ACE2 in vitro include Infection Invasion investigated Italy lysine mechanism Modification monomeric myocardial damage objective oligomerization Patient patients patients with diabete protease Protein rationale residue SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV2 Serine spike supplementary material susceptibility tissue TMPRSS2 TMPRSS2 protein Total transmembrane upregulation viral invasion was performed [DOI] 10.1186/s12933-021-01286-7 PMC 바로가기 [Article Type] Original Investigation
Rapid development of neutralizing and diagnostic SARS-COV-2 mouse monoclonal antibodies중화 및 진단용 SARS-COV-2 마우스 단일클론항체의 신속한 개발Article Published on 2021-05-062022-09-01 Journal: Scientific Reports [Category] SARS, 진단, [키워드] accelerated Amino acid angiotensin angiotensin converting enzyme Applied immunology binding cell types correlated COVID-19 pandemic Critical diagnostic enzyme epithelial cells Epitopes functional functions hACE2 immunization immunohistochemical staining immunology Infectious diseases Interaction Lung epithelial cells lung tissue mAb mAbs monoclonal antibody mouse monoclonal antibody Neutralizing Protein Rapid RBD reagent receptor Receptor binding domain Research S1 subunit SARS-CoV-2 screening process sequence Spike protein staining the S1 subunit the spike protein therapeutic Viral viral neutralization [DOI] 10.1038/s41598-021-88809-0 PMC 바로가기 [Article Type] Article
Fast Prediction of Binding Affinities of the SARS-CoV-2 Spike Protein Mutant N501Y (UK Variant) with ACE2 and Miniprotein Drug CandidatesSARS-CoV-2 스파이크 단백질 돌연변이 N501Y(영국 변이체)와 ACE2 및 미니단백질 약물 후보의 결합 친화도에 대한 빠른 예측Article Published on 2021-05-062022-09-11 Journal: The journal of physical chemistry. B [Category] MERS, SARS, 변종, 치료제, [키워드] ACE2 affinity angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 approach B.1.1.7 bind binding binding affinities binding affinity binding free energies binding free energy calculated candidate complex creating decrease demonstrated drug drug candidates drug resistance experimental structure explain free energies free energy Free energy calculations hACE2 host cells human ACE2 identified variant increase Lineage Lineage B.1.1.7 miniprotein mutant N501Y N501Y mutation overcome predict predicted prediction Protein Protein binding reported SARS-CoV-2 spike protein SARS-CoV-2 virus spike Spike protein the binding affinity the SARS-CoV-2 the spike protein therapeutic United Kingdom variant Viral viral entry [DOI] 10.1021/acs.jpcb.1c00869 PMC 바로가기 [Article Type] Article