Porcine deltacoronavirus causes diarrhea in various ages of field-infected pigs in ChinaResearch Articles Published on 2019-09-052022-10-28 Journal: Bioscience Reports [Category] Coronavirus, [키워드] acute diarrhea age anorexia Antigen bleeding caecum cause characterized China collected Colon conducted diarrhea hemorrhage Histopathology IHC immunohistochemistry infect intestinal intestinal wall Lymph node mesentery multiple organ Novel coronavirus PCR method PDCoV Pig age qRT-PCR real-time RT-PCR rectum Research RNA Sequencing spleen tissue watery [DOI] 10.1042/BSR20190676 PMC 바로가기 [Article Type] Research Articles
Porcine transmissible gastroenteritis virus inhibits NF-κB activity via nonstructural protein 3 to evade host immune systemResearch Published on 2019-08-052022-10-28 Journal: Virology Journal [Category] Coronavirus, MERS, [키워드] Activation amino acid residue analyzed canonical cause cells compose coronavirus Critical cytokine production Degradation demonstrated develop diarrhea domain dose-dependent inhibition downregulation enzyme activity evade exhibited family Coronaviridae gastroenteritis Host host immune system host innate immunity identify immunoprecipitation individual Infection inflammatory factors inhibit inhibiting inhibitory effect innate immune innate immune response involved IκBα modulating modulation mRNA expression NF-κB NF-κB NF-κB signaling non-structural protein nonstructural protein 3 Nsp3 nuclear nuclear translocation p65 Pathogenesis pathway Phosphorylation PLP PLP2 previous study Protein provide quantitative PCR reporter Result signaling pathway TGEV transmissible gastroenteritis virus ubiquitination Viral protein virus was used watery [DOI] 10.1186/s12985-019-1206-9 PMC 바로가기 [Article Type] Research
Superior immune responses induced by intranasal immunization with recombinant adenovirus-based vaccine expressing full-length Spike protein of Middle East respiratory syndrome coronavirusResearch Article Published on 2019-07-222022-10-28 Journal: PLoS ONE [Category] Coronavirus, MERS, [키워드] adenovirus-based Administered administration airway bind Bronchoalveolar lavage fluid candidate vaccine cause CD8 T cell Cellular immune response cellular receptor diarrhea Dipeptidyl peptidase 4 domain DPP4 exhibited expressing full-length glycoprotein highest humoral IgA antibody IgG antibody immune response immunization intramuscular intranasal intranasal route lung parenchyma measure memory MERS MERS-CoV MERS-CoV infection MERS-CoV S1 MERS-CoV vaccine Middle East N-terminal domain Neutralizing neutralizing antibody Neutralizing antibody titer neutralizing effect Protein Pseudotyped virus renal Respiratory illness respiratory syndrome coronavirus serum spike subunit Superior T cell response therapeutic Vaccine Vaccine development Vaccines vomiting [DOI] 10.1371/journal.pone.0220196 PMC 바로가기 [Article Type] Research Article
Single intranasal immunization with chimpanzee adenovirus-based vaccine induces sustained and protective immunity against MERS-CoV infectionArticle Published on 2019-05-252022-10-31 Journal: Emerging Microbes & Infections [Category] Coronavirus, MERS, [키워드] acute respiratory illness Adenovirus adenovirus-based antibody approved BALB/c mice cause cellular receptor chimpanzee adenoviral vector Critical demonstrated Dipeptidyl peptidase 4 DPP4 expresse full-length human DPP4 humans immune immunization immunized mice induce Interaction intranasal intranasal immunization MERS-CoV MERS-CoV infection MERS-CoV S protein MERS-CoV vaccine mice Middle East monoclonal antibody naïve Neutralizing activity neutralizing antibody passive transfer Prophylactic protective immune response protective immunity provided RBD Receptor binding domain receptor binding domain (RBD) respiratory robust sera serotype Spike protein survival sustained T cell response the S protein therapeutic intervention Vaccine Vaccine development viral entry [DOI] 10.1080/22221751.2019.1620083 PMC 바로가기 [Article Type] Article
Characteristics of the Life Cycle of Porcine Deltacoronavirus (PDCoV) In Vitro: Replication Kinetics, Cellular Ultrastructure and Virion Morphology, and Evidence of Inducing AutophagyArticle Published on 2019-05-182022-10-28 Journal: Viruses [Category] Coronavirus, MERS, [키워드] affected Alpha Analysis autophagy beta-coronavirus cause cells cellular Characteristics cycle cytoplasm described diarrhea electron microscopy evidence of genomic RNA help in vitro indicated Inducing Infection infectious titer Kinetics life membrane molecular mechanism morphology p62 porcine deltacoronavirus (PDCoV) Replication replication kinetics Structure ultrastructure Vesicle virion vomiting [DOI] 10.3390/v11050455 PMC 바로가기 [Article Type] Article
A Novel Immunochromatographic Strip for Antigen Detection of Avian Infectious Bronchitis VirusArticle Published on 2019-05-062022-10-29 Journal: International Journal of Molecular Sciences [Category] Coronavirus, MERS, [키워드] addition antibody antigen detection antigenic variation antigens cause demonstrated detection Detection limit dose facilitate Genotype Gold nanoparticles IBV ICS immunochromatographic Immunochromatographic strip infections Infectious Bronchitis Virus monoclonal antibodies monoclonal antibody novel nucleocapsid protein pathogen performed Rapid test reached respiratory viruses RT-PCR serotypes specificity Spread strip Surveillance Taiwan the disease variants viral evolution viral variant [DOI] 10.3390/ijms20092216 PMC 바로가기 [Article Type] Article
Attenuation of Infectious Bronchitis Virus in Eggs Results in Different Patterns of Genomic Variation across Multiple ReplicatesGenetic Diversity and Evolution Published on 2019-05-012022-10-30 Journal: Journal of Virology [Category] Coronavirus, MERS, [키워드] attenuate attenuation bronchitis cause Cell contagious coronavirus cultures develop Effective vaccines Egg Evolution Ex vivo exhibited Final genomic genomic variation growth High-throughput sequencing highlight IBV in vivo independent Infectious Bronchitis Virus kidney Live attenuated vaccine mechanism molecular mechanism Multiple Mutation nucleocapsid organ pattern phenotype Population raise replicate replicates required Respiratory disease Result sequence sequenced Single nucleotide polymorphism SNP SNPs structural protein tracheal Vaccine Vaccine design Vaccines Variation virulence virulent virus [DOI] 10.1128/JVI.00492-19 PMC 바로가기 [Article Type] Genetic Diversity and Evolution
UBXN1 interacts with the S1 protein of transmissible gastroenteritis coronavirus and plays a role in viral replicationResearch Article Published on 2019-04-272022-10-28 Journal: Veterinary Research [Category] Coronavirus, [키워드] Antiviral binding cause cDNA library Cell cells clone coronavirus Degradation diarrhea downregulated expression expression level gastroenteritis glycoprotein GST host receptor identify IFN-β in viral indicated Infection inhibition interact intestinal cell intracellular protein involved late phase morbidity and mortality negative control overexpression positive Protein protein domain protein synthesis provided regulate Replication Research responsible Sequencing sialic acid Signal transduction signaling pathways significantly significantly increased siRNA TGEV the S1 protein UBXN1 viral titer yeast [DOI] 10.1186/s13567-019-0648-9 PMC 바로가기 [Article Type] Research Article
Stage of Gestation at Porcine Epidemic Diarrhea Virus Infection of Pregnant Swine Impacts Maternal Immunity and Lactogenic Immune Protection of Neonatal Suckling PigletsImmunology Published on 2019-04-242022-11-01 Journal: Frontiers in Immunology [Category] Coronavirus, MERS, [키워드] acute diarrhea Affect affected antibody antibody secreting cell ASC B cells cause change circulating Control correlated Course Critical Cytokines determine during pregnancy epidemic diarrhea example gestation gut-mammary-secretory IgA axis IgA IgG immature immune system immune parameter immune protection immune response Immunity Impact implication Infection influence inoculated intestinal lactogenic Lactogenic immunity lymphocyte Maternal medium mucosal naïve Neonatal Neonate Neutralizing PEDV positively correlated Pregnancy pregnant protection required response resulting serum significantly significantly higher Stage stimulate stimulated subsequent Suckling survival survival rate swine three group Vaccines viral infections virulence virus [DOI] 10.3389/fimmu.2019.00727 PMC 바로가기 [Article Type] Immunology
Expression estimation and eQTL mapping for HLA genes with a personalized pipeline개인화된 파이프라인으로 HLA 유전자에 대한 발현 추정 및 eQTL 매핑Research Article Published on 2019-04-222022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] (Human Leukocyte Antigens abundance adaptive aligned allele antibody applied approach approaches balancing selection biases carried Causality cause choice classical HLA loci dataset datasets develop difficulty disease disease phenotype disease susceptibility Diseases Divergence downstream analyses downstream analysis DPA1 DPB1 DQA1 DQB1 DRB1 eQTL eQTL mapping eQTLs estimate estimates evaluate explain expressed expression expression level expression levels Gene Expression Genotype genotypes help HLA HLA allele HLA alleles HLA gene HLA genes HLA region HLA-A HLA-B HLA-C HLA-DPA1 HLA-DPB1 HLA-DQA1 HLA-DQB1 HLA-DRA HLA-DRB1 Human Leukocyte Antigens immune immune adaptive response influence information leukocyte Lineage lineages loci mapping nine organism P-value p-values paralogues phenotype Phenotypes predisposition problem qPCR quantification reference genome Regulation Regulatory remained RNA RNA-Seq sequence susceptibility target Transcriptome Variation [DOI] 10.1371/journal.pgen.1008091 PMC 바로가기 [Article Type] Research Article