Evaluation of an accelerated hydrogen peroxide disinfectant to inactivate porcine epidemic diarrhea virus in swine feces on aluminum surfaces under freezing conditionsResearch Article Published on 2017-12-012022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] accelerated Accelerated hydrogen peroxide addition aluminum applied collected Concentration condition conditions Contact Contamination control group country determine Dilution Disinfection Efficacy epidemic diarrhea evaluated expected fecal glycol hydrogen peroxide inactivated infectious dose Infectivity inoculated negative and positive negative control nucleocapsid PEDV polymerase chain positive positive control Quantitative Randomly rectal swab representing Result reverse transcription RT-qPCR Seven Spread supplementary material Swab swine temperature Treatment treatment group virus was determined were used [DOI] 10.1186/s12917-017-1300-4 PMC 바로가기 [Article Type] Research Article
Avian infectious bronchitis virus disrupts the melanoma differentiation associated gene 5 (MDA5) signaling pathway by cleavage of the adaptor protein MAVSResearch Article Published on 2017-11-132022-10-28 Journal: BMC veterinary research [Category] Coronavirus, MERS, [키워드] absence activate Activation Antiviral antiviral immune response bronchitis Cell cells cleavage cleave cytoplasmic disrupt downstream early stage exhibited host antiviral response IBV IBV infection IFN-β induce Infection infectious agent Infectious Bronchitis Virus influence Innate immunity interferon MAVS MDA5 Melanoma Melanoma differentiation associated gene 5 platform Protein question raise Result Retinoic acid-inducible gene-I RIG-I signaling pathway supplementary material susceptibility transfected with Viral RNA virus [DOI] 10.1186/s12917-017-1253-7 PMC 바로가기 [Article Type] Research Article
Limitations of using feline coronavirus spike protein gene mutations to diagnose feline infectious peritonitisResearch Article Published on 2017-10-052022-10-28 Journal: Veterinary Research [Category] Coronavirus, [키워드] Analysis Antigen coronavirus coronavirus spike protein determine diagnose Diagnosis faece Faeces fatal disease Feline infectious peritonitis gene mutation healthy histopathological identification immunohistochemistry implicated IMPROVE Infection limitation mutated Mutation positive Post-mortem Primers purified RNA RT-qPCR S gene Sample sensitivity Sequencing specificity Spread supplementary material tissue tissue sample viral spike was performed [DOI] 10.1186/s13567-017-0467-9 PMC 바로가기 [Article Type] Research Article
Identification of two mutation sites in spike and envelope proteins mediating optimal cellular infection of porcine epidemic diarrhea virus from different pathwaysResearch Article Published on 2017-08-302022-10-28 Journal: Veterinary Research [Category] Coronavirus, [키워드] aa deletion absence activate affected Analysis Apoptosis arginine cellular Characteristics Comparative conserved E protein eight envelope protein epidemic diarrhea ER stress expression Frame functional protein Genes genomic variation glycine GRP78 homologous host cell identification IL-6 IL-8 independent induce Infection membrane fusion morphology Mutation pathway PEDV potential mechanism pro-inflammatory cytokine proliferate proliferation protease Protein Receptor binding Replication responsible S2′ cleavage screened single mutation strain supplementary material transmembrane domain trypsin Variation Vero cells virion virus virus titer whole genome sequence α-coronavirus [DOI] 10.1186/s13567-017-0449-y PMC 바로가기 [Article Type] Research Article
Genome-wide analysis of codon usage bias in Bovine CoronavirusResearch Published on 2017-06-172022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] Analysis bovine codon codon usage comprehensive analysis coronavirus Evolution family Coronaviridae frequencies genus Betacoronavirus geographical distribution Host host gene host-pathogen interaction host’s immune system MOST mutational bia performed preference respiratory infection Result Significant strain supplementary material virus widespread [DOI] 10.1186/s12985-017-0780-y PMC 바로가기 [Article Type] Research
A reverse genetics system for avian coronavirus infectious bronchitis virus based on targeted RNA recombinationResearch Published on 2017-06-122022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] Accessory genes avian coronavirus cause Cell cell lines cells Characteristics chicken chimeric co-transfected coding Complete coronavirus ectodomain Embryonated eggs generate genetics Genome genomic growth IBV in vivo infect Infectious Bronchitis Virus isolates Major marker MHV Modification Molecular biology mouse hepatitis virus murine mutations parental poultry recombinant Recombination replaced respiratory pathogen Result Reverse genetics system RNA RNA recombination specific mutation spike gene spike glycoprotein strain Strains supplementary material synonymous Targeted RNA recombination Vaccine Vaccine development virulent virus was performed was used wild-type [DOI] 10.1186/s12985-017-0775-8 PMC 바로가기 [Article Type] Research
Detection of alpha- and betacoronaviruses in rodents from Yunnan, ChinaResearch Published on 2017-05-262022-10-28 Journal: Virology Journal [Category] Coronavirus, MERS, [키워드] Alpha alphacoronavirus alphacoronaviruses Analysis Apodemus Betacoronavirus characterized China collected conserved coronavirus Coronaviruses detection domains Eothenomy Evolution expected feature full-length genome functional genetic diversity Genome help Host infect intestinal investigated Lineage mammal much higher nucleocapsid protein nucleotide ORF3 ORF8 gene other coronavirus Pathogens Phylogenetic Rattus reservoir Result rodent S gene sequence similarity supplementary material Surveillance transcription regulatory sequence TRS upstream variant [DOI] 10.1186/s12985-017-0766-9 PMC 바로가기 [Article Type] Research
Two deletion variants of Middle East respiratory syndrome coronavirus found in a patient with characteristic symptomsAnnotated Sequence Record Published on 2017-04-182022-10-28 Journal: Archives of Virology [Category] Coronavirus, [키워드] Analysis anti-infection Antiviral bioinformatics characteristic China close contact confirmed case CoV Deletion deletion variant discharged dry cough E protein Effect Fever hospital individual Infectious Bronchitis Virus Inverted Repeat Sequence investigated mechanism MERS Middle East nasopharyngeal swab sample occurred ORF5 ORF5 Gene Patient previous study Protein reported respiratory syndrome coronavirus resulting sequence variation Sequencing Shortness of breath Significant supplementary material Symptom the patient therapy variant Virus Packaging was collected [DOI] 10.1007/s00705-017-3361-x PMC 바로가기 [Article Type] Annotated Sequence Record
Randomized, double-blind, placebo-controlled clinical trial to assess the safety and effectiveness of a novel dual-action oral topical formulation against upper respiratory infectionsResearch Article Published on 2017-01-142022-10-28 Journal: BMC Infectious Diseases [Category] Coronavirus, MERS, [키워드] age antimicrobial agent Antiviral ARMS Barrier formation cetylpyridinium chloride clinical trial Comparisons component conducted Contact coronavirus cough current demonstrated double-blind double-blinded Effectiveness Efficacy enrolled evaluate Frequency Glycerin had no Host individual Influenza Invasion medication mucosa not differ not significant occurred outcome pilot clinical trial placebo group placebo-controlled placebo-controlled clinical trial proof-of-concept clinical trial Prophylaxis Randomized reduced reducing reduction Registered reported representing Result rhinovirus Safe Secondary objective severity significantly sore throat statistically study outcomes study participant Study participants subject supplementary material the placebo group Tolerability treatment of influenza upper respiratory infection vaccination virus detection was reduced Xanthan gum [DOI] 10.1186/s12879-016-2177-8 PMC 바로가기 [Article Type] Research Article
Detection and phylogenetic analysis of porcine epidemic diarrhea virus in central China based on the ORF3 gene and the S1 geneResearch Published on 2016-11-252022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] addition Amino acid analysed Analysis China Chinese clinical samples Clustal detection diarrhea epidemic diarrhea full-length Genes genetic diversity glycoprotein insertion/deletion intestinal tissue isolate Japanese Korean Molecular epidemiology Mutation ORF3 ORF3 gene PEDV Phylogenetic analysis Phylogenetic tree Prevalence prevalent processed Protein provided Receptor binding Receptor binding domain Research Result RT-PCR S1 gene sequence Sequence analysis sequenced severity strain Strains supplementary material the disease the vaccine Vaccine variant variants virus [DOI] 10.1186/s12985-016-0646-8 PMC 바로가기 [Article Type] Research