Broad antiviral and anti‐inflammatory efficacy of nafamostat against SARS‐CoV‐2 and seasonal coronaviruses in primary human bronchiolar epitheliaResearch Article Published on 2021-06-302022-10-29 Journal: Nano Select [Category] COVID-19, [키워드] Activation airway epithelia airway epithelium anti‐inflammatory Antiviral antiviral activity antiviral effects antiviral property block bronchiolar Cell chronic obstructive COVID‐19 cytokine Donor Effect Efficacy epithelia epithelial cell evaluate exhibit healthy donors help Host host protease host serine protease human coronaviruses human coronaviruses 229E independent infect Inflammation inhibit log mitigate nafamostat Nafamostat mesylate observé Pathogenesis primary human airway pulmonary disease reduce reduction Replication respiratory infection SARS‐CoV‐2 SARS‐CoV‐2 seasonal coronavirus serine protease inhibitor smoker subject therapeutic therapeutic potential TMPRSS2 Treatment viral spike protein virus infection [DOI] 10.1002/nano.202100123 PMC 바로가기 [Article Type] Research Article
Development of a multiplex mass spectrometry method for simultaneous quantification of urinary proteins related to respiratory healthArticle Published on 2021-05-122022-10-04 Journal: Scientific Reports [Category] 바이오마커, [키워드] adverse effect airway epithelium binding Biomarker Biomarkers can be used cell protein characterized children children’s health complex confounder correlation development dysfunction Environmental impact facilitate handling Health health monitoring Human serum albumin immunoassay Lung disease multiplex Myoglobin NF-κB nine nuclear osteopontin peptide physiological Protein Proteins quantification ranged RBP4 renal renal dysfunction renal function reproducibility respiratory retinol selected Seven urinary dilution Urine Variation [DOI] 10.1038/s41598-021-89068-9 PMC 바로가기 [Article Type] Article
Genetic and non-genetic factors affecting the expression of COVID-19-relevant genes in the large airway epithelium대 기도 상피에서 COVID-19 관련 유전자의 발현에 영향을 미치는 유전 적 및 비 유전 적 요인Multicenter Study Published on 2021-04-212022-09-10 Journal: Genome Medicine [Category] MERS, SARS, 변종, 유전자 메커니즘, [키워드] ACE2 ACE2 binding ACE2 expression ACE2 gene active smoking affecting airway airway epithelium analyzed association Asthma Bronchial epithelium cardiovascular disease cause change chronic obstructive chronic obstructive pulmonary disease clinically Cohort cohorts contribute COPD COVID-19 COVID-19 severity COVID-19 susceptibility Defense determine disease course driven by enrichment analysis environmental factor epithelial eQTL eQTL mapping Evidence expression expression pattern expression patterns Factor Factors Gene Expression Gene Set Enrichment Analyses Genetic genetic factors Genetic variant Genetic variation Host host response hypertension identify immune response implicated individuals Inflammation inter-individual interferon investigated involved isoform non-binding obesity obstructive pulmonary disease performed phenotypic provide pulmonary disease Quantitative Regulation Regulatory replicated respiratory respiratory infections respiratory viruses Result risk factor RNA-sequencing SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 virus sequencing data smoker Smokers smoking subsequent supplementary material suppressed susceptibility the SARS-CoV-2 virus These data uninfected Variability variant variants Variation viruses [DOI] 10.1186/s13073-021-00866-2 PMC 바로가기 [Article Type] Multicenter Study
Effects of cigarette smoking on SARS-CoV-2 receptor ACE2 expression in the respiratory epithelium †호흡기 상피에서 SARS-CoV-2 수용체 ACE2 발현에 대한 흡연의 영향 †Comment Published on 2021-04-012022-09-11 Journal: The Journal of pathology [Category] SARS, 유전자 메커니즘, [키워드] ACE2 ACE2 expression ACE2 receptor acute respiratory syndrome coronavirus airway airway epithelium apical surface cellular entry cigarette smoke cigarette smoking coronavirus 2 COVID-19 COVID-19 symptom COVID-19 symptoms Effect experimental model experimental models future therapeutic gene expression datasets Great Britain highlight human lung tissue implication increase Ireland journal limitation Lung injury morbidity and mortality mortality rates murine model pandemic pathological pathology respiratory respiratory epithelium risk of transmission SARS-CoV-2 SARS-CoV-2 receptor Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 smoke smoking smoking cessation Society Sons structures virus [DOI] 10.1002/path.5607 PMC 바로가기 [Article Type] Comment
Amantadine Inhibits SARS-CoV-2 In VitroAmantadine은 시험관 내에서 SARS-CoV-2를 억제합니다Brief Report Published on 2021-03-242022-09-10 Journal: Viruses [Category] SARS, 치료제, [키워드] administration airway epithelium amantadine analogy antiviral activity antiviral agent Antiviral agents antiviral drug antiviral drugs approved Concentration COVID-19 demonstrated drug treatment drug treatments Efficacy Evidence Face mask Face masks health emergency Hypothesis in vitro infected with SARS-CoV-2 Influenza influenza A inhalation inhibited intranasal intranasal instillation mandatory Measures pandemic protective vaccine protective vaccines SARS-CoV-2 SARS-CoV-2 pandemic SARS-CoV-2 replication separate experiment severity social distancing tested the SARS-CoV-2 therapeutic Vaccine Vero E6 Vero E6 cell VERO E6 cells [DOI] 10.3390/v13040539 PMC 바로가기 [Article Type] Brief Report
Influence of Hypoxia on the Epithelial-Pathogen Interactions in the Lung: Implications for Respiratory Disease폐의 상피-병원체 상호작용에 대한 저산소증의 영향: 호흡기 질환에 대한 영향Review Published on 2021-03-242022-09-12 Journal: Frontiers in Immunology [Category] SARS, 진단, [키워드] Activation addition airway airway epithelium Asthma chemokines chronic inflammation chronic obstructive complex COVID-19 Defense disease dysfunction epithelial epithelial cells Evidence fibrosis highlight Host host-pathogen interaction host-pathogen interactions hypoxia hypoxia-inducible factor (HIF)-1 Immunity indicate Infection inflamed tissue Inflammatory inflammatory lung disease influence innate immune response Innate immunity Interaction lung modulating molecular mechanism mucosal normal physiological conditions novel respiratory disease oxygen pathophysiology physical Proinflammatory cytokine Protective provided pulmonary disease receptors Region respiratory Respiratory disease Respiratory pathogens response therapeutic target tissue [DOI] 10.3389/fimmu.2021.653969 PMC 바로가기 [Article Type] Review
Alpha-1 antitrypsin inhibits TMPRSS2 protease activity and SARS-CoV-2 infection알파-1 항트립신은 TMPRSS2 프로테아제 활성과 SARS-CoV-2 감염을 억제합니다Article Published on 2021-03-192022-09-10 Journal: Nature Communications [Category] SARS, 신약개발, 진단, 치료제, [키워드] acute phase airway epithelium Antiviral bind Bronchoalveolar lavage cell line cell lines circulating Concentration COVID-19 cultures Defense drug Factor fraction human airway epithelial identify infect inhibit inhibitor inhibitors inhibitors of SARS-CoV-2 innate immune knowledge membrane fusion Novel coronavirus physiological primary cell primary cells Protein respiratory respiratory pathogen respiratory tract SARS-CoV-2 SARS-CoV-2 entry SARS-COV-2 infection SARS-CoV-2 spike protein screened Serine serine protease serine protease inhibitor serine protease TMPRSS2 Spike protein suppresse the SARS-CoV-2 therapy TMPRSS2 TMPRSS2 protease Translational research Viral viral replication [DOI] 10.1038/s41467-021-21972-0 PMC 바로가기 [Article Type] Article
A Comparative Analysis of SARS-CoV-2 Antivirals Characterizes 3CL pro Inhibitor PF-00835231 as a Potential New Treatment for COVID-19Vaccines and Antiviral Agents Published on 2021-02-232022-10-30 Journal: Journal of Virology [Category] COVID-19, [키워드] 3CL pro A549 ACE2 acute respiratory syndrome airway airway epithelium alveolar Analysis Antiviral Antiviral compound antiviral drug approach approved Cell cells clades clinical trials Comparative complex component concentrations coronavirus coronavirus disease COVID-19 cultures develop direct-acting drug candidate effective Efficacy epithelial epithelial cell etiological agent Evidence experiment Express GC-376 Human airway epithelium human airway epithelium cultures in vitro in vitro model inhibit SARS-CoV-2 inhibitor kidney M pro New P-glycoprotein polymerase polyprotein Potential protease provide regimen Remdesivir SARS-CoV-2 SARS-CoV-2 life cycle SARS-CoV-2 protease Side effect suggested targets the SARS-CoV-2 the timing time-of-addition experiments treat VERO E6 cells viral life cycle [DOI] 10.1128/JVI.01819-20 PMC 바로가기 [Article Type] Vaccines and Antiviral Agents
Single cell resolution of SARS-CoV-2 tropism, antiviral responses, and susceptibility to therapies in primary human airway epithelium1차 인간 기도 상피에서 SARS-CoV-2 향성의 단일 세포 분해능, 항바이러스 반응 및 요법에 대한 감수성Research Article Published on 2021-01-282022-08-13 Journal: PLoS Pathogens [Category] Biochemistry, 치료제, [키워드] airway epithelial cells airway epithelium Antiviral antiviral immunity antiviral responses antiviral therapies antiviral therapy array blocked Cell cell population cell type cell types cellular ciliated cell ciliated cells Ciliated epithelial cells contribute COVID-19 pathogenesis dominant element elements epithelial cell epithelial cell type epithelial cell types epithelial cells expressed Factor flow cytometry gene signatures heterogeneity heterogenous host protease host protease TMPRSS2 Human airway epithelium hyperinflammatory responses IL-6 induction infect infected cell infected cells Infection inhibited initial interferon interferon response ISG ISGs mediator outcome Pathogenesis predominant primary human airway Remdesivir required respiratory respiratory tract RNA-sequencing SARS-CoV-2 SARS-COV-2 infection secretory cell secretory cells Single Cell susceptibility susceptible therapy These cells TMPRSS2 Treatment Treatment response variety Viral viral replication viral tropism [DOI] 10.1371/journal.ppat.1009292 PMC 바로가기 [Article Type] Research Article
A novel ACE2 isoform is expressed in human respiratory epithelia and is upregulated in response to interferons and RNA respiratory virus infection Research Published on 2021-01-112022-10-05 Journal: Nature Genetics [Category] 유전자 메커니즘, 진단, [키워드] ACE2 ACE2 binding ACE2 expression airway epithelial cell airway epithelium amino acids Cell contribute Critical enzyme expressed FIVE high-affinity binding Host host susceptibility identify in viral Infection interferon interferon stimulation isoform lack plasma membrane Protein respiratory epithelia respiratory virus resulting rhinovirus RNA RNA genome SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike the SARS-CoV-2 thought transcript Trigger unlikely upregulated viral infection [DOI] 10.1038/s41588-020-00759-x [Article Type] Research