SARS-CoV-2 spike linear epitope scanning via a peptide microarray through sera profiling혈청 프로파일링을 통한 펩타이드 마이크로어레이를 통한 SARS-CoV-2 스파이크 선형 에피토프 스캐닝Article Published on 2021-09-172022-09-12 Journal: STAR protocols [Category] SARS, 진단, [키워드] a peptide microarray acute respiratory syndrome antibody Antigen B-cell epitope Complete coronavirus coronavirus disease Diagnosis epitope Health Sciences High Throughput Screening humoral identification Immunological response immunology linear linear epitopes Microarray Microbiology Pathogens patients with COVID-19 protocol required SARS-CoV-2 SARS-CoV-2 spike sera Spike protein throughput Vaccine development [DOI] 10.1016/j.xpro.2021.100707 PMC 바로가기 [Article Type] Article
An in-depth in silico and immunoinformatics approach for designing a potential multi-epitope construct for the effective development of vaccine to combat against SARS-CoV-2 encompassing variants of concern and interest우려 및 관심의 변이를 포함하는 SARS-CoV-2에 대항하는 백신의 효과적인 개발을 위한 잠재적인 다중 에피토프 구성을 설계하기 위한 심층 실리코 및 면역정보학 접근 방식Article Published on 2021-09-012022-09-11 Journal: Computers in biology and medicine [Category] SARS, 변종, [키워드] ACE2 receptor acute respiratory syndrome acute respiratory syndrome coronavirus adaptive Antibody titer antigenicity approach B cell B cell epitope B-cell epitope B.1.1.7 B.1.351 B.1.427 B.1.429 B.1.526 B.1.617.1 B.1.617.2 binding Cell cells coronavirus COVID-19 effective elevated epitope Health immune response Immunity immunoinformatics in silico in vitro linker linkers MEV Multi-epitope vaccine NK cells nucleotide nucleotide sequence ORF1 ORF10 ORF1ab ORF3a ORF6 ORF7a ORF7b ORF8 outbreak P.1 P.2 Pathogens Physicochemical properties polyprotein present Prevent preventive measure receptor S protein SARS-CoV-2 SARS-CoV-2 protein SARS-CoV-2 proteins selected severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 T cell T cell epitope T-cell the vaccine TLR2 TLR4 Toll-like receptor Toll-like receptors turn utility Vaccine vaccine construct variants variants of concern variants of interest variants of SARS-CoV-2 viral pathogen were used [DOI] 10.1016/j.compbiomed.2021.104703 PMC 바로가기 [Article Type] Article
Revelation of Potent Epitopes Present in Unannotated ORF Antigens of SARS-CoV-2 for Epitope-Based Polyvalent Vaccine Design Using Immunoinformatics Approach면역정보학 접근법을 사용한 에피토프 기반 다가 백신 디자인을 위한 SARS-CoV-2의 주석이 없는 ORF 항원에 존재하는 강력한 에피토프의 계시Immunology Published on 2021-08-232022-09-11 Journal: Frontiers in Immunology [Category] MERS, SARS, 변종, 신약개발, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus allele Analysis antigen expression antigenic B-cell B-cell epitope B-cell epitopes canonical Canonical protein epitopes carried CD4+ T-cell CD8+ T-cell conformational coronavirus Coverage eight epitope epitope-based polyvalent vaccine Epitopes expressed FIVE followed by highest HLA I supergroups immunodominant immunogenic kill linear linear B-cell epitope linear B-cell epitopes linkers Mutation mutations N-protein Open reading frame open reading frames ORF ORF3c ORF7a Orf9b overlap Polyvalent potent present Protein Region regions respiratory revelation SARS-CoV-2 scenario severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Spike protein T-cell T-cell epitope T-cell epitopes the spike protein uORFs Vaccine variant variants [DOI] 10.3389/fimmu.2021.692937 PMC 바로가기 [Article Type] Immunology
NIgPred: Class-Specific Antibody Prediction for Linear B-Cell Epitopes Based on Heterogeneous Features and Machine-Learning ApproachesArticle Published on 2021-08-032022-10-28 Journal: Viruses [Category] COVID-19, MERS, SARS, [키워드] Accuracy Algorithm antibody Antigen applied approaches autocorrelation B-cell epitope Classifier coronavirus correlation epitope feature Feature selection Features foreign antigen Genetic help Heterologous identify IgA IgE IgG independent Invasion linear B-cell epitopes machine learning machine-learning Pathogens patients predict prediction Previous studies reducing specific antibody training model vaccine production website were used [DOI] 10.3390/v13081531 PMC 바로가기 [Article Type] Article
Structural Evaluation of the Spike Glycoprotein Variants on SARS-CoV-2 Transmission and Immune EvasionSARS-CoV-2 전달 및 면역 회피에 대한 스파이크 당단백질 변이체의 구조적 평가Review Published on 2021-07-102022-09-10 Journal: International Journal of Molecular Sciences [Category] SARS, 변종, 진단, [키워드] (Beta) ACE2 ACE2 receptor acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Alpha Alpha variant alteration angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 B-cell epitope B.1.1.7 B.1.351 B.1.427 B.1.429 B.1.526 B.1.617.2 Beta binding affinity caused Cluster conformational coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 death Delta domain Efficacy ENhance Epitopes Epsilon glycoprotein immune evasion immune responses include increase in induce information Iota lead Lineage lineages Mutation Neutralising Antibodies P.1 pandemic receptor reduction in respiratory S protein SARS-CoV-2 severe acute respiratory syndrome Coronavirus spike mutations Spike protein Spike proteins spike variants structures target the S protein the Spike Transmission vaccination Vaccine vaccine efficacy variants of SARS-CoV-2 Viral viral fitness [DOI] 10.3390/ijms22147425 PMC 바로가기 [Article Type] Review
Antibodies Targeting Two Epitopes in SARS-CoV-2 Neutralize Pseudoviruses with the Spike Proteins from Different VariantsCommunication Published on 2021-07-092022-10-28 Journal: Pathogens [Category] COVID-19, [키워드] Alpha variant Analysis antibody B-cell epitope Beta Beta variant caused COVID-19 COVID-19 pandemic D614G effective epitope IgG immunize information linear neutralization neutralize Particle peptide Point mutation prevalent Prevent produced Protective pseudovirus purified required SARS-CoV SARS-CoV-2 SARS-COV-2 infection Spike protein Spread targeting the Spike the spike protein Vaccine variant variants wild-type [DOI] 10.3390/pathogens10070869 PMC 바로가기 [Article Type] Communication
Multi-Epitope Vaccine Design Using an Immunoinformatic Approach for SARS-CoV-2Article Published on 2021-06-112022-10-28 Journal: Pathogens [Category] COVID-19, [키워드] Antigen applied B-cell epitope candidate caused CD19+ CD4+ CD8+ T-cell Cell Cellular immune response clinical trial COVID-19 death develop dissemination docking analysis Efficacy elicited epitope high mutation rate HLA alleles humans humoral Hydrogen bond identify IgG Immune escape immunize immunoinformatics in silico Approach Infection leukocyte lymph nodes lymphocyte mice multi-epitope peptide vaccine multiple epitope peptide peptide vaccine Prevent required SARS-CoV-2 selected serum significantly spleen T-cell T-cell epitopes the spike protein Vaccine variant viral genome [DOI] 10.3390/pathogens10060737 PMC 바로가기 [Article Type] Article
Identification of COVID-19 B-cell epitopes with phage-displayed peptide library파지 디스플레이 펩타이드 라이브러리로 코로나19 B 세포 에피토프 확인Research Published on 2021-06-072022-09-10 Journal: Journal of Biomedical Science [Category] MERS, SARS, 치료기술, 치료제, [키워드] antibody Antibody binding applied B-cell epitope B-cell epitopes BepiPred Care China city Consensus coronavirus coronavirus disease coronavirus disease 19 country COVID-19 COVID-19 pathogenesis COVID-19 patients Critical deaths determine diagnostic Diagnostic tool diagnostics enzyme enzyme-linked immunosorbent assay enzyme-linked immunosorbent assays epitope Epitopes facilitate globe identification immunological Medical care motif peptide peptides Phage-displayed peptides Protein Proteins PyMol residue Result SARS-CoV-2 SARS-CoV-2 viral sera serological Serological detection specific antibodies specific antibody spread of COVID-19 Structural analysis target targets transmitted two COVID-19 patients Vaccine Vaccines Viral Viral proteins virus was used Wuhan [DOI] 10.1186/s12929-021-00740-8 PMC 바로가기 [Article Type] Research
SARS-CoV-2 gene content and COVID-19 mutation impact by comparing 44 Sarbecovirus genomes44개의 Sarbecovirus 게놈을 비교하여 SARS-CoV-2 유전자 함량 및 COVID-19 돌연변이 영향Comparative Study Published on 2021-05-112022-09-10 Journal: Nature Communications [Category] SARS, 변종, 유전자 메커니즘, 치료법, [키워드] Analysis annotation B-cell epitope Cluster Comparative genomics conservation conserved contribute COVID-19 COVID-19 pandemic D614G Dissection E484K epitope evidence of Evolution except for experimental evidence functional mutation functional mutations Genome individual lack molecular evolution Mutation mutations N501Y Nsp3 nucleocapsid nucleotide ORF ORF3c ORF8 ORFs overlapping predicted protein-coding gene provide reported residue residues SARS-CoV-2 SARS-CoV-2 biology Sequence annotation spike-protein strain Substitution the SARS-CoV-2 Transmission valuable resource [DOI] 10.1038/s41467-021-22905-7 PMC 바로가기 [Article Type] Comparative Study
Comparison of Immunological Profiles of SARS-CoV-2 Variants in the COVID-19 Pandemic Trends: An Immunoinformatics ApproachArticle Published on 2021-05-062022-10-27 Journal: Antibiotics [Category] COVID-19, [키워드] antigenicity approach B-cell epitope circulating variant Combination combinations concerning COVID-19 COVID-19 cases COVID-19 pandemic COVID-19 severity descendant determine disease disease severity docking Efficacy England epidemiological epidemiological fitness epitope evaluated FIVE GISAID greater Hospital admission Human IEDB immune responses immunogenic profile immunoinformatics immunoinformatics approach immunological parameter increased risk India infection rate less moderate Mortality mutant Mutation NCBI Neutralizing neutralizing capacity non-allergic non-toxic pathogen Probability profile Protein protein sequence recorded reported SARS-CoV-2 SARS-CoV-2 antigenicity SARS-CoV-2 epitope prediction SARS-CoV-2 immunogenicity SARS-CoV-2 variant SARS-CoV-2 variants selected epitope sequence severe disease severity severity of COVID-19 simulation studies South Africa spike gene Spike protein T-cell the SARS-CoV-2 virus the spike protein the vaccine Toxicity translated Transmission USA vaccine modification variant variants Viremia virus Wuhan strain [DOI] 10.3390/antibiotics10050535 PMC 바로가기 [Article Type] Article