Occurrence of a novel cleavage site for cathepsin G adjacent to the polybasic sequence within the proteolytically sensitive activation loop of the SARS-CoV-2 Omicron variant: The amino acid substitution N679K and P681H of the spike proteinSARS-CoV-2 오미크론 변이체의 단백질 분해에 민감한 활성화 루프 내 다염기 서열에 인접한 카텝신 G에 대한 신규 절단 부위의 발생: 스파이크 단백질의 아미노산 치환 N679K 및 P681HArticle Published on 2022-04-182022-09-11 Journal: PLoS ONE [Category] COVID19(2023년), SARS, 변종, [키워드] activated neutrophil Activated neutrophils Activation adopted Amino acid amino acid substitution amino acids cleavage site delta variant furin host cell Infection innate immune response invading pathogens motif neutrophil Neutrophil elastase nsp4 Occurrence omicron Omicron variant P681H Pathogens peptide Peptide bond polybasic priming Protein proteinase resulting S1/S2 SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants secreted sequence Serine serine protease serine proteases Substitution the SARS-CoV-2 the Spike the spike protein Transmissibility transmission of SARS-CoV-2 variant variants viral surface virus [DOI] 10.1371/journal.pone.0264723 PMC 바로가기 [Article Type] Article
Functional Analysis of Spike from SARS-CoV-2 Variants Reveals the Role of Distinct Mutations in Neutralization Potential and Viral InfectivitySARS-CoV-2 변이체의 스파이크 기능 분석, 중화 가능성 및 바이러스 감염성에서 뚜렷한 돌연변이의 역할 밝혀Article Published on 2022-04-132022-09-11 Journal: Viruses [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] Affect Analysis approach carried circulating viral variants cleavage site conjunction COVID-19 defined Delta Disease progression dose E484K Efficacy enhanced escape FCS functional furin furin cleavage site furin–cleavage site in viral Infectivity Lota Mutation N501Y neutralization neutralization antibodies Neutralizing antibodies neutralizing antibody no effect no effects P681H P681R Pfizer Pfizer–BTN162b2 vaccine Potential promote pseudovirus pseudoviruses RBD RBD mutation RBD mutations Receptor binding domain receptor binding domain (RBD) reduce reveal role SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants sensitivity sera Site specific spike mutation specific spike mutations spike spike mutation spike mutations Spread vaccinated individuals Vaccine Viral viral infectivity Viral transmission viral variant Viral variants [DOI] 10.3390/v14040803 PMC 바로가기 [Article Type] Article
The “LLQY” Motif on SARS-CoV-2 Spike Protein Affects S Incorporation into Virus ParticlesSARS-CoV-2 스파이크 단백질의 "LLQY" 모티프는 S가 바이러스 입자에 통합되는 데 영향을 줍니다.Article Published on 2022-03-232022-09-11 Journal: Journal of Virology [Category] SARS, 변종, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus Affect Alter Amino acid amino acid residue amino acids antiviral drug antiviral drugs assembly biological significance Biosynthesis caused Cell culture cleavage cleavage site cleavage sites cleaved conformational conservation conserved Contact contribute coronavirus COVID-19 epidemic Critical demonstrated Effectiveness expressed glycoprotein hydrogen Hydrogen bond hydrogen bonds incorporation Infection intrachain junction key amino acid key amino acids less Lineage LLQY domain membrane fusion motif mutated Mutation Neutralizing antibodies Particle Prevent processing producer cell protease Protein protein synthesis protein synthesis and cleavage Pseudotyped virus pseudovirus pseudoviruses reduce residue S protein S1/S2 SARS-CoV-2 SARS-CoV-2 pseudoviruses SARS-CoV-2 variant SARS-CoV-2 variants sensitivity severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 spike Spike protein stability subcellular localization synthesis the S protein the SARS-CoV-2 Transmission tyrosine Vaccines viral entry viral infection virus virus assembly Virus-like particle virus-like particles [DOI] 10.1128/jvi.01897-21 PMC 바로가기 [Article Type] Article
Capturing a Crucial ‘Disorder-to-Order Transition’ at the Heart of the Coronavirus Molecular Pathology—Triggered by Highly Persistent, Interchangeable Salt-BridgesArticle Published on 2022-02-162022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] anionic appear arginine baseline being binding catalytic cationic characteristic cleavage site coronavirus COVID-19 disorder disorder-to-order transition enthalpy entropy compensation entropy arrest events Evolution feature FLCS flexible furin cleavage Genome heart homologous Host increase in Interaction interactions Lineage Molecular pathology motif multiplicity offer persistent polybasic Protein proteolytic proteolytic cleavage residue respiratory viral diseases respiratory viruses S1/S2 junction salt-bridge salt-bridge dynamics SARS-CoV SARS-CoV-2 spike Spike–Furin interaction the SARS-CoV-2 Transmissibility [DOI] 10.3390/vaccines10020301 PMC 바로가기 [Article Type] Article
Comparison of SARS-CoV-2 Evolution in Paediatric Primary Airway Epithelial Cell Cultures Compared with Vero-Derived Cell LinesArticle Published on 2022-02-052022-10-28 Journal: Viruses [Category] COVID-19, COVID19(2023년), SARS, [키워드] Adults age airway arising Cell Cell culture cell lines cells changes in children cleavage site co-morbidities Cohort Culture Delta dependent on driving epithelial Evidence Evolution Factor fixation Genetic genetic diversity genotypic growth highlight Host host cell factor host cells human respiratory epithelium in vitro infect isolate isolates LINE morbidity and mortality Mutation nasal passages phenotypic plasticity polybasic primary airway epithelial cells reduced remained respiratory epithelial cells resulting SARS-CoV-2 SARS-CoV-2 evolution several variant spike Strains variant Vero Vero cells viral evolution Virus evolution VoC whole-genome sequencing [DOI] 10.3390/v14020325 PMC 바로가기 [Article Type] Article
Combined insertion of basic and non-basic amino acids at hemagglutinin cleavage site of highly pathogenic H7N9 virus promotes replication and pathogenicity in chickens and miceArticle Published on 2022-02-012023-06-14 Journal: Virologica Sinica [Category] Fulltext, 조류인플루엔자, [키워드] Basic and non-basic amino acids cleavage site H7N9 highly pathogenic pathogenicity [DOI] 10.1016/j.virs.2022.01.001 PMC 바로가기
Cryptococcal Protease(s) and the Activation of SARS-CoV-2 Spike (S) ProteinArticle Published on 2022-01-272022-10-31 Journal: Cells [Category] COVID-19, [키워드] Cryptococcus neoformans activate biochemical cleavage site comparable cryptococcal infection Cryptococcus Efficiency fluorogenic peptide furin Infection peptide protease Protein proteolytic cleavage PRRA S protein S1 and S2 S1/S2 cleavage site S1/S2 site SARS-CoV-2 SARS-CoV-2 disease sequence spike spike (S) protein the SARS-CoV-2 unique [DOI] 10.3390/cells11030437 PMC 바로가기 [Article Type] Article
Evidence of a SARS-CoV-2 double Spike mutation D614G/S939F potentially affecting immune response of infected subjectsResearch Article Published on 2022-01-212022-10-31 Journal: Computational and Structural Biotechnology Journal [Category] COVID-19, [키워드] Abstract Affect affecting approach binding affinity binding epitope characterized cleavage site Community COVID-19 COVID-19 positive subject D614G D614G mutation deep sequencing diffusion effort epitope evidence of evidenced Fiumicino HLA alleles identify immune response implication Infection Italy modulation MONITOR mutated Mutation next generation sequencing objective peptide Protein Proteins reported respiratory sample Result S939F SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 variant SARS-CoV-2 variants selective Sequencing analysis severity specific mutation spike spike mutations stability subject T-cell triggered unique viral infection was performed was used [DOI] 10.1016/j.csbj.2022.01.021 PMC 바로가기 [Article Type] Research Article
The Emergence of the Spike Furin Cleavage Site in SARS-CoV-2Perspective Published on 2022-01-122022-10-29 Journal: Molecular Biology and Evolution [Category] COVID-19, SARS, [키워드] Alter cell type cleavage site coronavirus COVID-19 demonstrated emergence FCS furin cleavage site Human induce infect Interpretation Pathogenesis SARS-CoV-2 SARS-related coronavirus SARSr-CoVs selective Site stimulated the SARS-CoV-2 the Spike unique Virology [DOI] 10.1093/molbev/msab327 PMC 바로가기 [Article Type] Perspective
Structural insights in cell-type specific evolution of intra-host diversity by SARS-CoV-2Article Published on 2022-01-112022-10-04 Journal: Nature Communications [Category] SARS, 변종, 유전자 메커니즘, [키워드] ACE2 receptor binding addition amino-acid cell type cell types cleavage site Cryoelectron microscopy deletion variant domain eight Evolution functional furin recognition motif genetic diversity Host Intra-host diversity modulate molecular Molecular modelling pathology provide RNA virus S1/S2 cleavage site SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variant subpopulation Support trajectory Transmissibility variant viral cell Viral proteins viral variant virus infectivity wild-type [DOI] 10.1038/s41467-021-27881-6 PMC 바로가기 [Article Type] Article