Phosphate-regulated expression of the SARS-CoV-2 receptor-binding domain in the diatom Phaeodactylum tricornutum for pandemic diagnostics유행성 진단을 위한 규조류 Phaeodactylum tricornutum에서 SARS-CoV-2 수용체 결합 도메인의 인산염 조절 발현Article Published on 2022-04-292022-09-11 Journal: Scientific Reports [Category] COVID19(2023년), SARS, 변종, 진단, 치료기술, [키워드] alleviate anti-RBD antibiotic Antigen approach Betacoronavirus binding Biomedical research C-terminal can be used caused Cell coronavirus COVID-19 pandemic cross-reactive detect develop diagnostics domain Donor eukaryotic expressed expression expression plasmids finding glycosylated growth media histidine human ACE2 receptor inhibited iron lateral flow mammalian cell overexpression pandemic performed phosphate Plasmid polyclonal antibodies promoter purified RBD Receptor-binding domain retained SARS-CoV-2 SARS-CoV-2 proteins SARS-CoV-2 spike protein sensitivity serum specific IgG antibodies specific IgG antibody Spike protein Synthetic biology TEV protease the RBD the receptor-binding domain the SARS-CoV-2 Therapeutics Treatment type strain Viral antigen wild-type strain yield [DOI] 10.1038/s41598-022-11053-7 PMC 바로가기 [Article Type] Article
Structure-based dual affinity optimization of a SARS-CoV-1/2 cross-reactive single-domain antibodySARS-CoV-1/2 교차 반응성 단일 도메인 항체의 구조 기반 이중 친화성 최적화Article Published on 2022-03-302022-09-11 Journal: PLoS ONE [Category] MERS, SARS, 변종, 진단, [키워드] antibody applied approach binding binding affinity circulation coronavirus COVID-19 infections cross-reactive demonstrated dissociation rates exhibit functional fusion fusions host cell host cells human ACE2 receptor improvement Improvements Infection infections initiate Interaction leads lentivirus Live virus molecular mutant neutralization parental platform predicted Protein Protein binding pseudotyped S antigen SARS Coronavirus SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2 variant SARS-CoV-2 variants specificity Spike protein the SARS-CoV-2 treatments of COVID-19 variant VHH-72 VHH72 VHH72-Fc Viral viral entry Virus neutralization virus replication virus variants Wuhan [DOI] 10.1371/journal.pone.0266250 PMC 바로가기 [Article Type] Article
Omicron Variant of SARS-CoV-2 Virus: In Silico Evaluation of the Possible Impact on People Affected by Diabetes MellitusSARS-CoV-2 바이러스의 오미크론 변종: 당뇨병의 영향을 받는 사람들에게 미칠 수 있는 영향에 대한 실리코 평가Article Published on 2022-03-072022-09-11 Journal: Frontiers in Endocrinology [Category] SARS, 변종, 유전자 메커니즘, [키워드] ACE-2 receptor ACE2 ACE2 receptor affected Analysis approach asparagine B.1.1.529 binding cause Cell Characteristics computational analysis COVID-19 COVID-19 disease demonstration diabete diabetes Diabetes Mellitus entry routes evidenced feature finding Glycation higher affinity human ACE2 receptor human ACE2 receptor. Impact in silico Interaction interactivity lysine Maillard reaction maintain mechanism molecular Molecular mechanics mutant mutate Mutation omicron Omicron variant outcome Pathways RBD Receptor-binding domain residue risk SARS-CoV-2 severity shift shifting spike Spike protein surface area the disease the receptor-binding domain variant variant of SARS-CoV-2 virus virus attack wild-type [DOI] 10.3389/fendo.2022.847993 PMC 바로가기 [Article Type] Article
Evolutionary shift from purifying selection towards divergent selection of SARS-CoV2 favors its invasion into multiple human organsArticle Published on 2022-02-152022-10-04 Journal: Virus research [Category] COVID19(2023년), MERS, SARS, 변종, [키워드] Abstract Analysis appear CoV COVID-19 Critical Divergent selection evade Evolution Evolutionary rate favor genetic data Host human ACE2 receptor Image increase indicate Infection Invasion Lineage Mutation Neutral nonsynonymous mutation nucleotide substitution organ organs RaTG13 residue RNA virus S1 protein SARS-CoV2 SARS-CoV2 genome Substitution synonymous Temporal virus [DOI] 10.1016/j.virusres.2022.198712 PMC 바로가기 [Article Type] Article
Computational Analysis of Mutations in the Receptor-Binding Domain of SARS-CoV-2 Spike and Their Effects on Antibody BindingArticle Published on 2022-01-302022-10-28 Journal: Viruses [Category] COVID-19, SARS, [키워드] ACE2 ACE2 binding ACE2 receptor administration amino acid replacement Analysis analyzed antibody antigenic antigenic escape approaches binding binding affinity binding free energy bioinformatics Cluster complexes computational analysis computational mutagenesis coronavirus disease Coronaviruses COVID-19 Deleterious Effect epitope Epitopes feature hACE2 human ACE2 receptor human cell identify impairment indicate Infection Interaction monoclonal multiple mutation multiple mutations Mutation Neutralizing neutralizing antibody Pandemics performed produced Protein RBD RBD mutation RBD variant reduce resolved responsible S-protein SAR-CoV-2 SARS-CoV-2 sequence spike Strains Structure the RBD the receptor-binding domain the SARS-CoV-2 the vaccine Trigger Vaccines variants of SARS-CoV-2 virus–host interactions [DOI] 10.3390/v14020295 PMC 바로가기 [Article Type] Article
Mutations on RBD of SARS-CoV-2 Omicron variant result in stronger binding to human ACE2 receptorSARS-CoV-2 Omicron 변이체의 RBD 돌연변이는 인간 ACE2 수용체에 대한 더 강한 결합을 초래합니다Article Published on 2022-01-292022-09-11 Journal: Biochemical and Biophysical Research Communication [Category] SARS, 변종, [키워드] ACE2 ACE2-RBD Analysis bind binding binding interactions caused complex conducted COVID-19 pandemic death Efficacy ENhance human Ace2 protein human ACE2 receptor hydrogen Infection Interaction molecular dynamics Molecular dynamics simulation Mutation mutations neutralization antibodies Neutralization antibody occurred omicron Omicron mutant Omicron variant pandemic Protein raised RBD RBD of SARS-CoV-2 Receptor binding domain reducing S-protein SARS-CoV-2 SARS-CoV-2 virus solvent spike Spike protein surface area the SARS-CoV-2 virus Tight binding Vaccine variant variants of SARS-CoV-2 [DOI] 10.1016/j.bbrc.2021.12.079 PMC 바로가기 [Article Type] Article
Oral cavity infection by the SARS-CoV-2: emphasizing the essence of masking and peptide therapeuticsCorrespondence Published on 2022-01-102022-11-01 Journal: The Egyptian Journal of Medical Human Genetics [Category] COVID-19, [키워드] ACE2 ACE2 receptor binding blood pressure homeostasis carried cathepsin L cellular cleavage conformational change COVID-19 cytoplasm disease epithelial function functions glycoprotein healthcare high expression level high risk host’s body human ACE2 receptor Infection information inhibit nucleocapsid oral cavity oral mucosa other proteases peptide Protease inhibitor Protein proteolytic cleavage Regulation Saliva SARS-CoV-2 secretion supplementary material therapeutic TMPRSS2 viral loads viral spike [DOI] 10.1186/s43042-022-00213-z PMC 바로가기 [Article Type] Correspondence
Inferring the stabilization effects of SARS-CoV-2 variants on the binding with ACE2 receptorArticle Published on 2022-01-062022-10-04 Journal: Communications Biology [Category] SARS, 변종, [키워드] ACE2 receptor acute respiratory syndrome addition African amino acid substitution Analysis antigenicity binding characteristic complex complexes contagiousness coronavirus Effect English feature greater help human ACE2 receptor Interaction molecular Molecular conformation Molecular dynamics simulation Molecular modelling MONITOR Mutation negative control no effect observation occur pandemic performed phenotype phenotypic provoke residue SARS-CoV-2 spike protein SARS-CoV-2 spike variant SARS-CoV-2 variant selected selective several variant South specific mutation spike variant spike variants Spread stability stabilizing effect Surveillance the SARS-CoV-2 the spike protein variant variants Variation viral genome virus while wild type [DOI] 10.1038/s42003-021-02946-w PMC 바로가기 [Article Type] Article
Therapeutic potential of melatonin and melatonergic drugs on K18-hACE2 mice infected with SARS-CoV-2SARS-CoV-2에 감염된 K18-hACE2 마우스에 대한 멜라토닌 및 멜라토닌성 약물의 치료 가능성Article Published on 2022-01-012022-09-11 Journal: Journal of pineal research [Category] SARS, 변종, 신약개발, [키워드] administration agomelatine benefit candidate changes in Clinical outcome clinical trials Compound COVID-19 COVID-19 pandemic COVID-19 pathogenesis Cytokines Daily disease dose drug Drug repurposing drug treatment drug treatments drugs effective exacerbated expressing hACE2 human ACE2 receptor humans infected with SARS-CoV-2 Infection Inflammatory cytokine Inflammatory cytokines interferons K18-hACE2 K18-hACE2 mice limit Local lung melatonin mice Occurrence preclinical study Prophylactic ramelteon ramelteon. Safe SARS-CoV-2 SARS-COV-2 infection shown Spread survival susceptible Symptom tested therapeutic therapeutic potential treat treated Treatment type I type I and type III interferons type III Type III interferons vaccination variant [DOI] 10.1111/jpi.12772 PMC 바로가기 [Article Type] Article
The fatty acid site is coupled to functional motifs in the SARS-CoV-2 spike protein and modulates spike allosteric behaviourCommunications Published on 2021-12-112022-10-31 Journal: Computational and Structural Biotechnology Journal [Category] COVID-19, [키워드] Abstract ACE2, angiotensin-converting 2 enzyme Affect Alter binding binding pocket binding site CD, connector domain CH, central helix change conformational Contact D614G FA, fatty acid fatty FP, fusion peptide FPPR, fusion-peptide proximal region functional furin cleavage site Genetic variation host cell HR1, heptad repeat 1 human ACE2 receptor identify LA, linoleic acid Ligand MD, molecular dynamics membrane fusion MERS, Middle East respiratory syndrome modulate Molecular dynamics simulation motif mutant Mutation NTD, N-terminal domain Protein RBD, receptor binding domain RBM, receptor-binding motif receptor-binding motif Region regions removal RMB, receptor binding motif SARS-CoV-2 spike protein SARS-CoV-2, severe acute respiratory syndrome 2 SARS, Severe acute respiratory syndrome Science significantly Simulation Spike protein the SARS-CoV-2 the SARS-CoV-2 virus transmitted variant [DOI] 10.1016/j.csbj.2021.12.011 PMC 바로가기 [Article Type] Communications