The Na + , K + -ATPase β 1 subunit regulates epithelial tight junctions via MRCK αResearch Article Published on 2021-02-222022-10-30 Journal: JCI Insight [Category] Coronavirus, [키워드] Actin Activation acute respiratory distress alveolar ARDS caused Cell Biology demonstrated downstream drug target dysfunction epithelial expressed expression Gene Expression Gene therapy homeostasis human airway Infection Influenza virus lung lung epithelial mechanism modulation myotonic overexpression Patient Protein protein interaction Proteins pulmonary disease Pulmonology reduced regulate Regulation required SARS-CoV-2 subunit syndrome tight junction tight junctions upregulate [DOI] 10.1172/jci.insight.134881 PMC 바로가기 [Article Type] Research Article
Proposal of selective wedge instillation of pulmonary surfactant for COVID-19 pneumonia based on computational fluid dynamics simulationTechnical Advance Published on 2021-02-222022-10-28 Journal: BMC Pulmonary Medicine [Category] COVID-19, [키워드] airway alveolar Alveolar collapse ARDS assigned assumed Bronchiole bronchioles Bronchoscopic therapy calculated caused Cell Clinical use clinically Concentration COVID-19 Diffuse alveolar damage distress driving dysfunction effective End-Expiratory Pressure human airway interstitial pneumonia investigated lobular Lung fibrosis observation performed Pneumonia Prevent Pulmonary edema Region regions replaced Result secrete selective subsegmental syndrome therapy Type Type II pneumocyte Vascular ventilator was determined Wedge pressure [DOI] 10.1186/s12890-021-01435-4 PMC 바로가기 [Article Type] Technical Advance
Inhaled corticosteroids downregulate the SARS-CoV-2 receptor ACE2 in COPD through suppression of type I interferon흡입 코르티코스테로이드는 I형 인터페론의 억제를 통해 COPD에서 SARS-CoV-2 수용체 ACE2를 하향 조절합니다Covid-19 Published on 2021-02-012022-09-10 Journal: The Journal of Allergy and Clinical Immunology [Category] MERS, SARS, 유전자 메커니즘, [키워드] Abstract ACE2 ACE2 expression ACE2, Angiotensin-converting enzyme-2 administration airway angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme-2 BEC, Bronchial epithelial cell BSG, Basigin Cell culture changes chronic obstructive chronic obstructive pulmonary disease contribute COPD COPD, Chronic obstructive pulmonary disease coronavirus disease Coronavirus disease 2019 Corticosteroids COVID-19 COVID-19 in patient COVID-19, Coronavirus disease 2019 disease downregulate Elastase entry receptor epithelial epithelial cell evaluate the effect evaluated exogenous IFN expression FP, Fluticasone propionate functional GR, Glucocorticoid receptor group human airway human airway epithelial ICS ICS therapy ICS, Inhaled corticosteroid ICSs IFN-α/β IFN-β IFNAR1 Inhaled inhaled corticosteroid inhaled corticosteroids interferon signaling ISG, Interferon-stimulated gene mechanism mice mouse model Mouse models objective obstructive pulmonary disease Patient PROTECT pulmonary disease receptor receptor ACE2 reduce expression reduced Result reversed risk SARS-CoV-2 entry receptor severe coronavirus disease severe COVID-19 sputum stratified susceptibility the SARS-CoV-2 therapy TMPRSS2, Transmembrane serine protease 2 type I type I interferon Viral viral entry viral infection were used [DOI] 10.1016/j.jaci.2020.09.034 PMC 바로가기 [Article Type] Covid-19
HTCC as a Polymeric Inhibitor of SARS-CoV-2 and MERS-CoVSARS-CoV-2 및 MERS-CoV의 고분자 억제제로서의 HTCCArticle Published on 2021-01-282022-09-11 Journal: Journal of Virology [Category] 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 antiviral activity bats block Care characterization China chitosan chloride circulating Cluster Compound Coronaviridae coronavirus demonstrated Entry epithelia escaped ex vivo model HCoV HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 Health Health care highly pathogenic HKU1 host cell HTCC human airway human airway epithelia human coronavirus humans in vitro infect information inhibited inhibition inhibitor Interaction life-threatening Local measure mechanism mechanism of action member MERS MERS-CoV Middle East Middle East respiratory syndrome Coronavirus multiorgan failure NL63 Novel coronavirus OC43 pandemic pathogen Polymer Rapid Research respiratory respiratory infection respiratory syndrome coronavirus S protein SARS-CoV-2 SARS-COV-2 infection Seven severe acute respiratory syndrome Coronavirus Spread the S protein virus virus entry viruses [DOI] 10.1128/JVI.01622-20 PMC 바로가기 [Article Type] Article
Off-Target In Vitro Profiling Demonstrates that Remdesivir Is a Highly Selective Antiviral Agent오프 타겟 시험관 내 프로파일링은 렘데시비르가 고도로 선택적인 항바이러스제임을 보여줍니다Article Published on 2021-01-202022-09-11 Journal: Antimicrobial Agents and Chemotherapy [Category] 치료제, [키워드] 50% effective concentration addition adenosine aerobic agent Anti-SARS-CoV-2 Activity Antiviral Antiviral agents assays biochemical biochemical assay cells cellular cellular respiration Concentration coronavirus COVID-19 cytotoxicity demonstrated DNA DNA and RNA Effect elicit epithelial cells evaluated Filovirus filoviruses GS-5734 human airway human airway epithelial human airway epithelial cells human cell human cell line human cell lines human cells inhibitor Interaction Last MERS MERS-CoV metabolic conditions metabolite Mitochondria mitochondrial mitochondrial DNA mitochondrial respiration nucleoside nucleoside analogs oxygen polymerases primary cells Profiling protein synthesis ranged RDV reactive oxygen species Remdesivir reported resulting safety profile SARS-CoV SARS-CoV-2 selective selectivity indices target tested Toxicity Treatment triphosphate turn Viral Viral RNA [DOI] 10.1128/AAC.02237-20 PMC 바로가기 [Article Type] Article
SARS-CoV-2 entry into human airway organoids is serine protease-mediated and facilitated by the multibasic cleavage siteResearch Article Published on 2021-01-042022-10-31 Journal: eLife [Category] COVID-19, [키워드] acute respiratory syndrome airway Airway organoids bind blocking Calu-3 cell Cathepsins Cell cell lines cleavage coronavirus COVID-19 endosomal entry facilitated faster host proteases human airway in vivo increase inhibited MBCs multibasic cleavage site Organoid plasma membrane protease receptor Replication SARS-CoV SARS-CoV-2 SARS-CoV-2 entry Serine serine protease serine proteases Syncytia the SARS-CoV-2 the spike protein viral entry virus [DOI] 10.7554/eLife.64508 PMC 바로가기 [Article Type] Research Article
Deposition distribution of the new coronavirus (SARS-CoV-2) in the human airways upon exposure to cough-generated droplets and aerosol particlesArticle Published on 2020-12-312022-10-29 Journal: Scientific Reports [Category] COVID-19, MERS, SARS, [키워드] ACE2 acute respiratory syndrome addition airway airways angiotensin-converting enzyme 2 applied binding bystander Cell cellular entry Clinical deterioration coronavirus cough coughs COVID-19 deposition direct infection disease distribution droplet effort expressed Fluid dynamics form human airway infect infected patient infected with SARS-CoV-2 Infection initial symptom less majority Mild Model nasal New coronavirus non-specific oral mucosa pandemic parameter Particle Particles pathogen Pathogenesis Pneumonia Probability produced progression quarantine receptor reducing Respiration respiratory droplet SARS-CoV-2 SARS-COV-2 infection significantly sneezes Spread stochastic Symptom target target cells the disease thought tissue Transport Treatment upper airway upper airways upper respiratory tract viral infection Viral load virus while [DOI] 10.1038/s41598-020-79985-6 PMC 바로가기 [Article Type] Article
Host metabolism dysregulation and cell tropism identification in human airway and alveolar organoids upon SARS-CoV-2 infectionSARS-CoV-2 감염 시 인간의 기도 및 폐포 오르가노이드에서 숙주 대사 조절 장애 및 세포 친화성 식별Research Article Published on 2020-12-122022-09-10 Journal: Protein & Cell [Category] MERS, SARS, 유전자 메커니즘, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition airway alveolar animals AT2 Cancer cells caused Cell cell line cell lines cell tropism cells cellular metabolism ciliated club Complete coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 Culture distal downregulation Drug discovery dysregulation embryonic embryonic stem cells human airway human lung organoid human lung organoids humans immune response in vitro infect infected cell infected cells Infection inhibited lipid metabolism lung organoids Lungs mechanism metabolic processes metabolism neutralizing antibody Organoid organoids organs pandemic pathophysiological physiological condition physiological conditions proximal Remdesivir replicated respiratory respiratory droplet Respiratory droplets RNA-Seq route SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 replication severe acute respiratory syndrome Coronavirus Spread stem cell supplementary material therapeutic drug upregulation virus infection [DOI] 10.1007/s13238-020-00811-w PMC 바로가기 [Article Type] Research Article
Inhibition of Coronavirus Entry In Vitro and Ex Vivo by a Lipid-Conjugated Peptide Derived from the SARS-CoV-2 Spike Glycoprotein HRC DomainResearch Article Published on 2020-10-202022-10-29 Journal: mBio [Category] COVID-19, MERS, SARS, [키워드] 2019 coronavirus disease acute respiratory syndrome airways alleviation of symptoms block C-terminal Cell cell monolayer cell-cell fusion coronavirus COVID-19 cultures death described detectable domain etiological agent Evidence Ex vivo exhibit fusion inhibitor global pandemic glycoprotein HAE Health care system heptad repeat highlight host cell membranes HRC human airway human airway epithelial humans in vitro Infection inhibit inhibiting inhibition inhibitor initial lipopeptide live SARS-CoV-2 medical intervention MERS-CoV Middle East monolayers mortalities other coronaviruses peptide Prophylaxis respiratory syndrome coronavirus respiratory viral resulting S glycoprotein SARS-CoV-1 SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 S SARS-CoV-2 S glycoprotein spike Spike protein Spread structural rearrangement the SARS-CoV-2 therapeutic therapy tissue treat treated Treatment Vaccine Vero E6 viral spread Viral transmission while widespread [DOI] 10.1128/mBio.01935-20 PMC 바로가기 [Article Type] Research Article
Evidence-Based Considerations Exploring Relations between SARS-CoV-2 Pandemic and Air Pollution: Involvement of PM2.5-Mediated Up-Regulation of the Viral Receptor ACE-2Article Published on 2020-08-022022-10-29 Journal: International Journal of Environmental Research an [Category] COVID-19, MERS, [키워드] ACE-2 affecting AhR Air pollution Analysis angiotensin-converting enzyme 2 Aryl hydrocarbon receptor association Bioinformatic bioinformatic analysis cell receptor cellular response Concentration Consensus contributing to correlation Correlation analysis COVID-19 DNA sequence environmental factor epidemiological data evaluate fatality Fatality rate favor geographical areas Health human airway Hypothesis identify incidence inter-individual Involvement Italian mechanism mortality rate motif nine outcome over-expression pandemic PM2.5 relation risk of COVID-19 SARS-CoV-2 SARS-COV-2 infection severity Spread Support the SARS-CoV-2 transcription factor Variability was performed xenobiotic response element [DOI] 10.3390/ijerph17155573 PMC 바로가기 [Article Type] Article