Genotyping of the Major SARS-CoV-2 Clade by Short-Amplicon High-Resolution Melting (SA-HRM) AnalysisSA-HRM(Short Amplicon High-Resolution Melting) 분석을 통한 주요 SARS-CoV-2 분기군 유전자형 분석Article Published on 2021-04-052022-09-10 Journal: Genes [Category] MERS, SARS, 변종, [키워드] allele-specific RT-PCR Analysis approach B.1.1.28 B.1.1.7 clade clinical feature Clinical features country COVID-19 pandemic defined derivatives distribution equipment Features Genome Genotype genotyping High-resolution human pathogen Major melting multiple mutation multiple mutations Mutation NGS outcome patients predicted Prevent profiles SA-HRM Saliva Sanger sequencing SARS-CoV-2 SARS-CoV-2 clades SARS-CoV-2 virus SARS-CoV-2 viruses sequence Sequencing specific clade specific clades the SARS-CoV-2 virus two group two groups variant variants virus viruses [DOI] 10.3390/genes12040531 PMC 바로가기 [Article Type] Article
Prefusion spike protein stabilization through computational mutagenesis전산 돌연변이 유발을 통한 Prefusion 스파이크 단백질 안정화Article Published on 2021-04-012022-09-11 Journal: Proteins [Category] SARS, 변종, [키워드] ACE2 acute respiratory syndrome angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 automatically binding biochemical Bioinformatics analysis computational mutagenesis conserved contribute coronavirus Critical death develop Eris global pandemic human cell human cells human pathogen identify molecular molecular dynamics molecular dynamics simulations Mutagenesis mutant mutants Mutation predict prefusion Protein Protein design protein stabilization Proteins residue residues resulting SARS-CoV-2 Severe acute respiratory syndrome significantly Spike protein stability Structural analysis the spike protein thermodynamic Vaccine viral-host interaction [DOI] 10.1002/prot.26025 PMC 바로가기 [Article Type] Article
Natural selection in the evolution of SARS-CoV-2 in bats created a generalist virus and highly capable human pathogen박쥐에서 SARS-CoV-2의 진화에서 자연 선택은 일반 바이러스와 고도로 유능한 인간 병원체를 만들었습니다Research Article Published on 2021-03-122022-08-13 Journal: PLoS Biology [Category] Biology and Life Sciences, Fulltext, SARS, 유전자 메커니즘, 조류인플루엔자, 치료기술, [키워드] accompanied acting acute respiratory syndrome coronavirus adaptive Analysis ancestor Anti-viral Antiviral base bat bat Sarbecovirus bats can not Cell Coinfection coronavirus coronavirus 2 coronavirus disease Coronavirus disease 2019 COVID-19 CpG CpG composition detect early phase Evidence evidence of Evolution Evolution of SARS-CoV-2 horseshoe bats Host hosts Human human pathogen humans include involved Lineage lineages mechanism moderate natural natural selection nucleotide nucleotides pandemic positive Positive selection progenitor recombinant Recombination required respiratory reveal RmYN02 Sarbecoviruses SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Support the cells the early SARS-CoV-2 Transmission virus viruses while [DOI] 10.1371/journal.pbio.3001115 PMC 바로가기 [Article Type] Research Article
SARS‐CoV‐2 infection remodels the host protein thermal stability landscapeSARS-CoV-2 감염은 숙주 단백질 열 안정성 풍경을 개조합니다Article Published on 2021-02-162022-09-10 Journal: Molecular Systems Biology [Category] MERS, 치료제, [키워드] abundance acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition alteration aryl hydrocarbon hydroxylase biosafety Cell cell cycle cellular cellular processe cellular processes change changes in condition coronavirus displaying effective framework global change handling Health heat shock chaperone Host host protein host proteins human pathogen imperative Infection Interaction Microbiology, Virology & Host Pathogen Interaction microtubule molecular Pathogens Protein proteome provide Regulation Replication respiratory rhapontigenin RNA splicing SARS‐CoV‐2 SARS‐CoV‐2 infection SARS‐CoV‐2 severe acute respiratory syndrome Coronavirus stability suppressed tanespimycin targets therapy thermal thermal stability Vaccine [DOI] 10.15252/msb.202010188 PMC 바로가기 [Article Type] Article
Berberine and Obatoclax Inhibit SARS-Cov-2 Replication in Primary Human Nasal Epithelial Cells In VitroArticle Published on 2021-02-112022-10-28 Journal: Viruses [Category] COVID-19, MERS, [키워드] Acidic acute respiratory syndrome affected Air-liquid interface anti-apoptotic Antiviral compounds antiviral drug berberine chemokines complement Concentration concentrations coronavirus COVID-19 cytokine early stage effective Efficacy Endosomes expression Human human pathogen increase in indicated Infection inhibited isolate late stage less nasal epithelial cell neutralize Obatoclax preventive measures primary target cells Protein reduced repressed SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 replication Vaccines viral life cycle viral RNA synthesis [DOI] 10.3390/v13020282 PMC 바로가기 [Article Type] Article
Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiationSARS-CoV-2 NSP1과 인간 리보솜의 mRNA 간의 동적 경쟁은 번역 개시를 억제합니다408 Published on 2021-02-092022-09-10 Journal: Proceedings of the National Academy of Sciences of [Category] MERS, 유전자 메커니즘, [키워드] 40S absence accommodation acute respiratory syndrome acute respiratory syndrome coronavirus beta-CoV beta-CoVs bind binding biochemical biochemically causative agent cellular complexes coronavirus COVID-19 pandemic determine disrupt downstream dynamic eukaryotic translation eukaryotic translation initiation facilitate Factor Factors focus Host human pathogen human ribosome Infection inhibit initiation factors Interaction Messenger RNA modulate modulated molecular MONITOR mRNA mRNAs nsp1 open Prevent Protein protein synthesis real time required respiratory ribosomal subunit ribosome RNA SARS-CoV-2 SARS-CoV-2 NSP1 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Significance single-molecule fluorescence start codon subunit Support translation translation initiation virulence virulence factor virus viruses [DOI] 10.1073/pnas.2017715118 PMC 바로가기 [Article Type] 408
Packaging Covered with Antiviral and Antibacterial Coatings Based on ZnO Nanoparticles Supplemented with Geraniol and CarvacrolArticle Published on 2021-02-092022-10-29 Journal: International Journal of Molecular Sciences [Category] COVID-19, MERS, [키워드] active against active coatings active packaging analyzed Antibacterial antibacterial properties antiviral activity antiviral properties assumed bacteriophage coating Coronaviruses Covered explain Geraniol Gram-negative bacteria human pathogen indicated Measures moderate nanoparticle phi 6 phage Respiratory droplets SARS-Co-V2 SARS-CoV-2 selected synergistic Testing transmitted virus viruses [DOI] 10.3390/ijms22041717 PMC 바로가기 [Article Type] Article
A Versatile Reporter System To Monitor Virus-Infected Cells and Its Application to Dengue Virus and SARS-CoV-2바이러스 감염 세포를 모니터링하는 다목적 리포터 시스템과 뎅기열 바이러스 및 SARS-CoV-2에 대한 적용Article Published on 2021-01-282022-09-11 Journal: Journal of Virology [Category] 진단, 치료기술, [키워드] Antiviral antiviral activity Antiviral compounds application blocks can be used caused Cell cells characterization cleavage cleave Coronaviridae Coronaviruses Dengue dengue virus develop disease disease outbreaks disease severity effective etiological agent expressed Flaviviridae flexibility fluorescent fused GFP globe host cell human pathogen identify include infected cell infected cells Infection limit live cell imaging molecular nuclear nuclear translocation nucleoside nucleus outbreak pandemic Pathogens proof protease Protease activity Proteases Protein Quantitative reporter reporter cell lines reporter system Research RNA virus SARS-CoV-2 sequence severity Spread System the Coronaviridae these viruses this system translocate Viral viral proteases viral replication virus virus spread Virus-host interaction Virus-host interactions virus-infected cell virus-infected cells viruses Visualization Zika Zika virus [DOI] 10.1128/JVI.01715-20 PMC 바로가기 [Article Type] Article
The evolutionary dynamics of endemic human coronavirusesRapid Communication Published on 2021-01-202022-10-28 Journal: Virus Evolution [Category] COVID-19, [키워드] accumulated acute respiratory syndrome adaptive Affect amino acid change analyzed antigenic antigenic variant average Bayesian Bayesian framework Cell changes in coronavirus Coronavirus-2 dataset Endemic endemic human coronavirus Evidence Evolution evolutionary dynamics Evolutionary rate expected gene regions Genes genetic variability glycoprotein H3N2 HCoV-229E HCoV-OC43 highest Host HPD human coronavirus human coronaviruses human pathogen IAV influenza A virus influenza vaccines less likelihood maximum mutations neutralizing antibody responses Non-synonymous mutation occurred pandemic Phylogeny Positive selection protective immunity RBD Receptor binding domain representing reservoir RNA virus S gene SARS-CoV-2 SARS-CoV-2 evolution similarities similarity stability Substitution supported target the RBD Vaccine viral entry virus [DOI] 10.1093/ve/veab020 PMC 바로가기 [Article Type] Rapid Communication
Factors enforcing the species boundary between the human pathogens Cryptococcus neoformans and Cryptococcus deneoformans인간 병원체 크립토코커스 네오포르만스와 크립토코커스 데네오포르만스 사이의 종 경계를 강화하는 요인Research Article Published on 2021-01-192022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] Alternative pathway Aneuploidy Bacterial block component Cryptococcus Cryptococcus neoforman Cryptococcus neoformans deleting determine DNA DNA sequence DNA sequences eukaryote eukaryotes Factor fecundity Frequency fungal Genetic genetic recombination human pathogen hybrid hybridization identify increase in incubated instance interspecific interspecific hybrid interspecific hybrids isolate lack lead limit Loss maintain mechanism medium meiosis meiotic recombination Msh2 parent parental parents pathogenic pathway phenotype Previous studies previous study produced progeny Recombination Sequencing sexual reproduction strain Structure structures transgressive phenotype transgressive phenotypes unique Variation viability whole-genome whole-genome sequencing yeast [DOI] 10.1371/journal.pgen.1008871 PMC 바로가기 [Article Type] Research Article