Effect of Ivermectin and Atorvastatin on Nuclear Localization of Importin Alpha and Drug Target Expression Profiling in Host Cells from Nasopharyngeal Swabs of SARS-CoV-2- Positive PatientsSARS-COV-2- 양성 환자의 비 인두 면봉으로부터의 숙주 세포에서 수입 알파 및 약물 표적 발현 프로파일 링에 대한 Ivermectin 및 Atorvastatin의 효과Article Published on 2021-10-152022-09-10 Journal: Viruses [Category] SARS, 신약개발, 유전자 메커니즘, 치료법, 치료제, [키워드] Actin Affect alteration analyzed antihelmintic drug atorvastatin Cdc42 cellular Combination concentrations confocal microscopy COVID-19 COVID-19 patient COVID-19 patients cytoplasmic distribution drug Drug repurposing drug target drugs Effect expression function Gene Expression host cell host cell antiviral response host cells impair Importin in vitro model Infection inhibit involved Ivermectin KPNA3 KPNA5 KPNA7 KPNB1 lipophilic statin Localization mechanism Nasopharyngeal swab nasopharyngeal swabs NF-κB NF-κB activation Non-COVID-19 patients nuclear Nuclear localization Pathogenesis Patient pleiotropic effect poly(I:C) Profiling Result Rho GTPase Rho GTPases RHOA RNA viruses SARS-CoV-2 SARS-COV-2 infection Signaling target transcriptional Transport Treatment triggered Vesicle Viral viral double-stranded RNA [DOI] 10.3390/v13102084 PMC 바로가기 [Article Type] Article
Highly Efficient SARS-CoV-2 Infection of Human Cardiomyocytes: Spike Protein-Mediated Cell Fusion and Its Inhibition인간 심근세포의 고효율 SARS-CoV-2 감염: 스파이크 단백질 매개 세포 융합 및 억제Virus-Cell Interactions Published on 2021-10-062022-09-10 Journal: Journal of Virology [Category] MERS, SARS, 변종, [키워드] 2019 aberrant host response accounted ACE2 Activation activity Acute respiratory infection acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Amino acid angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 Autopsy block canonical cardiac cardiac complication Cardiac complications cardiac damage cardiac tropism cardiovascular complication cardiovascular complications catalytic site Cell Cell fusion cell lines cell surface cell-cell fusion cells cleavage cleavage site coding coronavirus coronavirus disease correlated COVID-19 COVID-19 patient cytopathic Cytopathic effect cytoplasm cytoplasmic tail direct infection disrupt Drug discovery efficient examined exceeded Express expressed expression vector fluorescent furin furin cleavage motif furin-like protease furin-like proteases fused fusing fusion fusion inhibition green fluorescent protein Human human cardiomyocyte in vitro model inactive Infection inhibition Lungs mechanism monomeric mRNA mutant Mutation Myocardium occur other coronavirus other coronaviruses parental Particle patients peptide-based platform pluripotent stem cell-derived cardiomyocytes protease Protein protein cleavage Proteolytic processing receptor replicate required respiratory respiratory infection SARS-CoV-2 SARS-COV-2 infection Serine serine 2 severe acute respiratory syndrome Coronavirus spike Spike protein Spread structures Syncytia systemic inflammation the spike protein These data thrombosis tissue TMPRSS2 Transformed cell transmembrane transmembrane protease serine 2 tropism of SARS-CoV-2 Vesicle Viral viral replication viral transcript viral transcription viral transcripts Virus-like particle virus-like particles viruses [DOI] 10.1128/JVI.01368-21 PMC 바로가기 [Article Type] Virus-Cell Interactions
Immunohistochemistry for protein detection in PFA-fixed paraffin-embedded SARS-CoV-2-infected COPD airway epitheliumPFA 고정 파라핀 포매 SARS-CoV-2 감염 COPD 기도 상피에서 단백질 검출을 위한 면역조직화학Article Published on 2021-09-172022-09-11 Journal: STAR protocols [Category] SARS, 치료기술, 치료법, [키워드] Air-liquid interface airway airway epithelium antibody Cell chronic lung disease chronic obstructive COPD differentiation epithelial Health Sciences immunology in vitro model in vivo Lung disease Microbiology Microscopy NHBE paraffin-embedded Patient Protein protocol protocols provide SARS-CoV-2 SARS-COV-2 infection severe disease [DOI] 10.1016/j.xpro.2021.100663 PMC 바로가기 [Article Type] Article
Two-Step In Vitro Model to Evaluate the Cellular Immune Response to SARS-CoV-2Article Published on 2021-08-262022-10-31 Journal: Cells [Category] COVID-19, MERS, SARS, [키워드] A549 A549 cell activated antiviral activity antiviral genes Apoptosis can be used caused CD4 Cellular immune response contributed COVID-19 Culture Effect Effects evaluate expressing feature IFNg immune Immunity in vitro in vitro model individual induce Inflammatory cytokine lung cell line memory Model monocyte mononuclear cell natural killer PBMC Peripheral blood primary cell raised reduce response SARS-CoV-2 SARS-CoV-2 antigen Spike protein subject supernatant T cell these cell Vaccine were infected were used [DOI] 10.3390/cells10092206 PMC 바로가기 [Article Type] Article
Combination Treatment With Remdesivir and Ivermectin Exerts Highly Synergistic and Potent Antiviral Activity Against Murine Coronavirus Infection렘데시비르와 이버멕틴의 병용 치료는 쥐 코로나바이러스 감염에 대해 높은 시너지 효과와 강력한 항바이러스 활성을 나타냅니다.Cellular and Infection Microbiology Published on 2021-07-302022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] MERS, SARS, 치료제, [키워드] activity Against antiviral drug antiviral therapy Betacoronavirus Betacoronavirus genus both viruses Cell Chloroquine clinical clinical trial clinical trials Combination combination therapy combination treatment coronavirus COVID-19 COVID-19 pandemic cytokine level develop doxycycline drug drug therapy effective Efficacy evaluated exhibited Hepatitis highest IC50 IL-6 in vitro model in vivo experiment indicated infect Infection inhibitory investigated Ivermectin ivermectin treatment leukemia Live virus lowest Macrophage macrophages mechanism MHV mice monotherapy murine hepatitis virus potent RAW264.7 macrophage cells reduction Remdesivir Replication Safe SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections selectivity index sequence identity significant reductions synergism synergistic tested the Betacoronavirus the disease therapy TNF-α Treatment Trial urgency viability Viral Viral RNA virus viruses [DOI] 10.3389/fcimb.2021.700502 PMC 바로가기 [Article Type] Cellular and Infection Microbiology
IFITM proteins promote SARS-CoV-2 infection and are targets for virus inhibition in vitroArticle Published on 2021-07-282022-10-04 Journal: Nature Communications [Category] SARS, 변종, [키워드] anti-viral activity antibody binding block Brain cells cofactor Coronaviruses dissemination Effect expression Gut human cell human lung human lung cells IFITM in vitro in vitro model in vivo Infection inhibit inhibit SARS-CoV-2 Innate immunity interact interferon organoids overexpression Pathogenesis peptide promote Protein Replication reported representing restrict SARS-CoV-2 SARS-COV-2 infection spike Support target the SARS-CoV-2 therapeutic approaches transmembrane protein viral infection viral pathogens Viral transmission virus entry virus inhibition viruses were expressed [DOI] 10.1038/s41467-021-24817-y PMC 바로가기 [Article Type] Article
Emerging Approaches to Understanding Microvascular Endothelial Heterogeneity: A Roadmap for Developing Anti-Inflammatory TherapeuticsReview Published on 2021-07-212022-10-29 Journal: International Journal of Molecular Sciences [Category] COVID-19, [키워드] Affect alteration blood vessel bMFA cellular and molecular Developing differentially expressed endothelial barrier permeability Endothelial cell Endothelium expressed functional variation Hemostasis heterogeneity in vitro model in vivo Inflammation inflammatory disease inner layer intrinsic leukocyte leukocytes Microphysiological systems microvascular endothelial cells Morphological neutrophil recruitment organ organ damage organ-specific organs phenotypic protein kinase Cδ proteomics reactive oxygen specy regulate regulated Regulation RNA sequencing Sepsis systemic systemic inflammatory response Therapeutics transmigration understanding Variation Vascular endothelium [DOI] 10.3390/ijms22157770 PMC 바로가기 [Article Type] Review
In Vitro Models for Studying Entry, Tissue Tropism, and Therapeutic Approaches of Highly Pathogenic CoronavirusesReview Article Published on 2021-06-212022-10-27 Journal: BioMed Research International [Category] COVID-19, [키워드] binding cell entry cell line cell receptor cellular protease CoV CoVs ENCODE family Coronaviridae Genome host cell in vitro model information inhibiting knowledge membranes methodology Model offer Pathogenesis positive-sense Protein RNA virus RNA viruses structural proteins the S protein the Spike therapeutic approaches therapeutic intervention tropism viral tropism [DOI] 10.1155/2021/8856018 PMC 바로가기 [Article Type] Review Article
In Vitro Models for Studying Entry, Tissue Tropism, and Therapeutic Approaches of Highly Pathogenic CoronavirusesReview Article Published on 2021-06-212022-10-27 Journal: BioMed Research International [Category] Coronavirus, COVID-19, MERS, SARS, [키워드] binding cell entry cell line cell receptor cellular protease CoV CoVs ENCODE family Coronaviridae Genome host cell in vitro model information inhibiting knowledge membranes methodology Model offer Pathogenesis positive-sense Protein RNA virus RNA viruses structural proteins the S protein the Spike therapeutic approaches therapeutic intervention tropism viral tropism [DOI] 10.1155/2021/8856018 PMC 바로가기 [Article Type] Review Article
Immunomodulation: Immunoglobulin Preparations Suppress Hyperinflammation in a COVID-19 Model via FcγRIIA and FcαRI면역 조절 : 면역 글로불린 제제는 FcγRIIA 및 FCαRI를 통해 COVID-19 모델에서 과인 염증을 억제합니다.Immunology Published on 2021-06-102022-08-31 Journal: Frontiers in Immunology [Category] MERS, SARS, 바이오마커, 진단, [키워드] “cytokine storm” Activation addition Anti-inflammatory antibody cells characterized Clinical outcome coated with convalescent plasma COVID-19 COVID-19 disease COVID-19 pathology cytokine cytokine release Cytokine storm dissect drug dysregulated immune response elicit Fc-receptors FcγR followed by form functional global pandemic highlighting HL60 human immunoglobulin IgA IgG IgG and IgM IgM antibodies immune complex immune modulation immune modulatory drug immune modulatory drugs immune response Immunoglobulin immunoglobulins IMPROVE in vitro model include Inflammation Inflammatory Intravenous immunoglobulin ITAMi IVIG Model neutrophil Neutrophils pathology patients phagocytosis preparation reduced SARS-CoV-2 severe COVID-19 patients severe forms of COVID-19 Signaling Spike protein Spread tested therapeutic trimodulin [DOI] 10.3389/fimmu.2021.700429 PMC 바로가기 [Article Type] Immunology