Long-Term Potato Virus X (PVX)-Based Transient Expression of Recombinant GFP Protein in Nicotiana benthamiana Culture In VitroArticle Published on 2021-10-152023-08-29 Journal: Plants [Category] E형 간염, [키워드] green fluorescent protein (GFP) Nicotiana benthamiana plant tissue culture potato virus X (PVX) recombinant proteins transient expression Viral vector [DOI] 10.3390/plants10102187 PMC 바로가기 [Article Type] Article
The Major Peanut Allergen Ara h 2 Produced in Nicotiana benthamiana Contains Hydroxyprolines and Is a Viable Alternative to the E. Coli Product in Allergy DiagnosisOriginal Research Published on 2021-10-042023-08-30 Journal: Frontiers in Plant Science [Category] E형 간염, [키워드] Ara h 2 hydroxyproline Nicotiana benthamiana Peanut allergy PTM transient expression [DOI] 10.3389/fpls.2021.723363 PMC 바로가기 [Article Type] Original Research
Highly active engineered IgG3 antibodies against SARS-CoV-2SARS-CoV-2에 대한 고활성 조작된 IgG3 항체403 Published on 2021-10-012022-09-10 Journal: Proceedings of the National Academy of Sciences of [Category] MERS, SARS, 변종, 진단, [키워드] addition antibodies antibody Antigen antigen binding antigen-binding site binding binding activity complex protein domain engineered IgG3 exhibited expressed expression fucose function glycosylation homogeneous human serum IgG subclasse IgG subclasses IgG1 IgG3 mAb mAbs monoclonal antibodies neutralization neutralization potency neutralize Nicotiana benthamiana plant plant-based expression planta plants protective effect SARS-CoV-2 SARS-CoV-2 immunity SARS-COV-2 infection SARS-CoV-2 spike protein Spike protein subtype therapy Vaccine development variant Viral viral surface Virus neutralization xylose [DOI] 10.1073/pnas.2107249118 PMC 바로가기 [Article Type] 403
Impact of Specific N -Glycan Modifications on the Use of Plant-Produced SARS-CoV-2 Antigens in Serological AssaysPlant Science Published on 2021-09-272022-10-31 Journal: Frontiers in Plant Science [Category] COVID-19, [키워드] allergen allergic antibodies antibody Antigen average carbohydrate Cell characterized comparable contribute COVID-19 cross-reactive cross-reactive carbohydrate determinant detect determinant epitope event expressed expression False positive False positives glycosylated glycosylation human cell human sera humans immune response Impact individual Interaction Modification Neutralizing antibodies Nicotiana benthamiana pandemic plant populations posttranslational modification produced RBD reactivity Receptor binding domain reduced required SARS-CoV-2 sera serological assays serological test Specific Spike protein the SARS-CoV-2 Transient variant viral antigens viral infection virus was used while wild-type [DOI] 10.3389/fpls.2021.747500 PMC 바로가기 [Article Type] Plant Science
Plant-Expressed Receptor Binding Domain of the SARS-CoV-2 Spike Protein Elicits Humoral Immunity in MiceArticle Published on 2021-09-012022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] accounted caused Coronavirus disease-19 COVID-19 death distribution effective elicited expressed glycoengineering highlight Humoral immunity immunogens IMPROVE mice Neutralizing antibodies neutralizing antibody Nicotiana benthamiana offer pandemic plant plant specific glycosylation plant-based vaccine platform Protein RBD RBD antigen Receptor binding domain receptor binding domain (RBD) Recombinant protein required robust Safe SARS-CoV-2 SARS-CoV-2 vaccine spike subunit the SARS-CoV-2 transient expression utility Vaccine Vaccine development vaccine production Volume [DOI] 10.3390/vaccines9090978 PMC 바로가기 [Article Type] Article
Plant-Produced Glycosylated and In Vivo Deglycosylated Receptor Binding Domain Proteins of SARS-CoV-2 Induce Potent Neutralizing Responses in MiceArticle Published on 2021-08-122022-10-28 Journal: Viruses [Category] COVID-19, SARS, [키워드] ACE2 antibody antigens Antiviral binding caused country COVID-19 COVID-19 pandemic elicited enzyme global public health glycosylated greater high risk immunized in vivo knowledge mice monoclonal antibody Neutralizing activity Nicotiana benthamiana plant potent prevention of COVID-19 produced Protein purification purified RBD RBD antigen RBD protein RBD proteins RBD-specific antibody reactivity receptor Receptor binding domain receptor binding domain (RBD) vaccine response Safe SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 RBD SARS-CoV-2 vaccines Spike protein spread to Support the SARS-CoV-2 therapeutic drug transient expression system vaccine candidate vaccine candidates virus-neutralizing [DOI] 10.3390/v13081595 PMC 바로가기 [Article Type] Article
Plant-produced SARS-CoV-2 receptor binding domain (RBD) variants showed differential binding efficiency with anti-spike specific monoclonal antibodiesResearch Article Published on 2021-08-112022-10-28 Journal: PLoS ONE [Category] COVID-19, MERS, SARS, [키워드] ACE2 acute respiratory syndrome Anti-spike Beta binding binding affinity binding domain binding efficiency characterized contrary convalescent coronavirus disease Coronavirus-2 COVID-19 CR3022 death ELISA enzyme exhibited Host host cell receptor increase in mAb mAbs monoclonal antibody Mutation neutralization neutralizing antibody Nicotiana benthamiana other variants pandemic produced raise RBD RBD-Fc receptor reported required Respiratory illness responsible SARS-CoV-2 SARS-CoV-2 RBD SARS-CoV-2 RBD protein SARS-CoV-2 receptor sera severe pneumonia South Africa strain suggested the spike protein transient expression United Kingdom vaccinated individual variant [DOI] 10.1371/journal.pone.0253574 PMC 바로가기 [Article Type] Research Article
The C-Terminal Half of SARS-CoV-2 Nucleocapsid Protein, Industrially Produced in Plants, Is Valid as Antigen in COVID-19 Serological TestsPlant Science Published on 2021-07-272022-10-31 Journal: Frontiers in Plant Science [Category] COVID-19, [키워드] Affect antibody Antigen biofactories calculated city coronavirus disease Council COVID-19 COVID-19 diagnosis develop Diagnosis diagnostic diagnostic test diagnostic tests ELISA ELISA coronavirus enzyme-linked immunosorbent expressed expression human sera individual Laboratory molecular farming N antigen N protein Nicotiana benthamiana pandemic plant plant expression plants platform positive result producing protein antigen purification reagent Recombinant protein Research Result RT-PCR SARS CoV-2 nucleocapsid protein SARS-CoV-2 SARS-CoV-2 antibody sensitivity Sensitivity and specificity sera serological shown specificity subset Support Test tested the N protein turnaround times was used were used [DOI] 10.3389/fpls.2021.699665 PMC 바로가기 [Article Type] Plant Science
N-Glycosylation of the SARS-CoV-2 Receptor Binding Domain Is Important for Functional Expression in PlantsPlant Science Published on 2021-06-152022-10-31 Journal: Frontiers in Plant Science [Category] COVID-19, [키워드] ACE2 Amino acid antibody benthamiana binding cellular receptor characterized complex convalescent sera COVID-19 CR3022 enzyme expressed glycoprotein glycosylated glycosylation growth factor had no Host monoclonal antibodies monomer N-glycan N-glycosylation Nicotiana benthamiana plant posttranslational modification Protein protein expression RBD Receptor binding domain Recombinant protein recombinant proteins SARS-CoV-2 secreted site-directed mutagenesis Spike protein the SARS-CoV-2 variant Variation Viral antigen virus while [DOI] 10.3389/fpls.2021.689104 PMC 바로가기 [Article Type] Plant Science
Plant-Produced Receptor-Binding Domain of SARS-CoV-2 Elicits Potent Neutralizing Responses in Mice and Non-human PrimatesPlant Science Published on 2021-05-132022-10-31 Journal: Frontiers in Plant Science [Category] COVID-19, [키워드] acute respiratory syndrome administration affected affinity column approved caused clinical trials coronavirus coronavirus disease COVID-19 demonstrated domain effective elicited enzyme-linked immunospot assay expressed Fc fragment Fc fusion protein fused global public health human IgG1 IFN-γ immune response immunized mice intramuscular investigated knowledge mice neutralization titer Nicotiana benthamiana Non-human non-human primates plant-produced recombinant protein potent primate Protein protein A purified RBD RBD of SARS-CoV-2 RBD-Fc RBD-Fc fusion protein Receptor-binding domain response SARS-CoV-2 SARS-CoV-2 RBD SARS-CoV-2 RBD protein spreads Stage subunit Subunit vaccine the disease Vaccine vaccine candidate [DOI] 10.3389/fpls.2021.682953 PMC 바로가기 [Article Type] Plant Science