High throughput sequencing based direct detection of SARS-CoV-2 fragments in wastewater of Pune, West India인도 서부 푸네의 폐수에서 SARS-CoV-2 조각의 높은 처리량 시퀀싱 기반 직접 검출Article Published on 2022-02-102022-09-11 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] affected Analysis ARTIC protocol asymptomatic individual Asymptomatic individuals circulating Clinical data clinically concern conducted COVID-19 pandemic delta variant detect Early detection epidemiological Epidemiology escaped hypothesised identify India infections Lineage metagenomics Mutation mutations nanopore sequencing nsp14 nucleic acid Occurrence pandemic rapid increase reported responsible SARS-CoV-2 SARS-CoV-2 infected individuals SARS-CoV-2 mutation SARS-CoV-2 mutations SARS-CoV-2 nucleic acid SARS-CoV-2 virus Sequencing utility valuable resource variant virus wastewater West [DOI] 10.1016/j.scitotenv.2021.151038 PMC 바로가기 [Article Type] Article
SARS-CoV-2 detection in nasopharyngeal swabs: Performance characteristics of a real-time RT-qPCR and a droplet digital RT-PCR assay based on the exonuclease region (ORF1b, nsp 14)비인두 면봉에서 SARS-CoV-2 검출: 엑소뉴클레아제 영역(ORF1b, nsp 14)을 기반으로 한 실시간 RT-qPCR 및 비말 디지털 RT-PCR 분석의 성능 특성Article Published on 2022-02-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] Accuracy agreement analyzed assays CDC center Chain Reaction Characteristics Combination Control diagnostic tests disease control droplet Droplet digital RT-PCR e genes exonuclease Genome investigated nasopharyngeal Nasopharyngeal swab nasopharyngeal swabs nsp14 ORF1b performance polymerase chain reaction positive prevention protocols provided qPCR RdRP Real-time RT-qPCR request for RT-PCR RT-PCR assay RT-qPCR SARS-CoV-2 SARS-CoV-2 detection specificity Spread Support Swab [DOI] 10.1016/j.jviromet.2021.114420 PMC 바로가기 [Article Type] Article
N7-Methylation of the Coronavirus RNA Cap Is Required for Maximal Virulence by Preventing Innate Immune RecognitionResearch Article Published on 2022-01-252022-10-29 Journal: mBio [Category] COVID-19, COVID19(2023년), MERS, SARS, [키워드] ACE2 acute respiratory syndrome Brain CAP caused comparable Complete contribute coronavirus coronavirus disease CoV COVID-19 disease effective vaccine exhibited expression guanine IFN-I immune response immunological in vitro Infection Innate interferons ISG15 liver MDA5 MHV mice morbidity and mortality mouse hepatitis virus mutant mutant virus Mutation myeloid cell N7-methylation nonstructural protein 14 nonstructural protein 14 (nsp14) nsp14 pandemic Pathogenesis phenotype Prevent Preventing previous study Recognition reduced replicated required restored RNA RNA cap structure RNA methylation SARS-CoV-2 SARS-COV-2 infection Signaling significantly subsequent the vaccine therapy transgenic mice type I interferon understanding upregulated viral replication Viral RNA virulence wild-type [DOI] 10.1128/mbio.03662-21 PMC 바로가기 [Article Type] Research Article
Coronaviral RNA-methyltransferases: function, structure and inhibitionAcademicSubjects/SCI00010 Published on 2022-01-252022-10-31 Journal: Nucleic Acids Research [Category] COVID-19, [키워드] Activation antiviral immunity complex Coronaviral COVID cryo-EM structure cytoplasm Degradation enzyme inhibitor inhibitors innate immune system Innate immunity methyltransferase nsp14 pandemic RNA scrutiny stability viral replication Viral RNA X-ray [DOI] 10.1093/nar/gkab1279 PMC 바로가기 [Article Type] AcademicSubjects/SCI00010
Detection of SARS-CoV-2 RNA in Bivalve Mollusks by Droplet Digital RT-PCR (dd RT-PCR)Droplet Digital RT-PCR(dd RT-PCR)에 의한 이매패류 연체동물의 SARS-CoV-2 RNA 검출Article Published on 2022-01-142022-09-10 Journal: International Journal of Environmental Research an [Category] SARS, 변종, 치료기술, [키워드] Alpha Alpha variant approach assays B.1.1.7 bivalve Campania characteristic collected Concentration COVID-19 detectable detection Digital displaying droplet feces FIVE human pathogen impacted infected patient infected patients Lineage Lineage B.1.1.7 median Mollusk MONITOR Mutation nsp14 outbreak Partial Pathogens PCR positive sample Prevalence real-time RT-PCR RT-PCR SARS-CoV-2 SARS-CoV-2 RNA Sequencing shellfish tested variant Viral virus was obtained wastewater water water bodies water body waters [DOI] 10.3390/ijerph19020943 PMC 바로가기 [Article Type] Article
Activation of the SARS-CoV-2 NSP14 3′–5′ exoribonuclease by NSP10 and response to antiviral inhibitorsResearch Article Published on 2021-12-202022-10-30 Journal: The Journal of Biological Chemistry [Category] COVID-19, SARS, [키워드] activate acute respiratory syndrome Analysis antiviral inhibitor antiviral therapeutics biochemical C-terminal complex coronavirus COVID-19 COVID-19, Coronavirus disease 2019 CV, column volume DDEE, residues forming the active site aspartic acid (D) and glutamic acid (E) DMSO, dimethyl sulfoxide DSF, differential scanning fluorimetry ENhance enzyme enzyme kinetics Escherichia coli exonuclease exonucleases Exoribonuclease expression Factor FL, full-length highlight increase in inhibit inhibitor methyltransferase monophosphate MP, monophosphate MTase, methyltransferase N-terminal Ni, nickel nonstructural protein 14 NP-40, Nonidet-P40 nsp10 nsp14 NSP14, nonstructural protein 14 NTA, nitrilotriacetic acid observé producing Protein protein complex protein drug interaction Protein purification protein stability protein–protein interaction Proteins protocol provide purification Remdesivir Replication required RMP, remdesivir monophosphate SAH, S-adenosylhomocysteine SARS-CoV-2 SARS-CoV-2 replication SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 SEC, size-exclusion chromatography small molecule TCEP, Tris(2-carboxyethyl)phosphine the SARS-CoV-2 the SARS-CoV-2 genome Treatment understanding viral proofreading Viral protein [DOI] 10.1016/j.jbc.2021.101518 PMC 바로가기 [Article Type] Research Article
Discovery of SARS-CoV-2 Nsp14 and Nsp16 Methyltransferase Inhibitors by High-Throughput Virtual ScreeningArticle Published on 2021-11-302022-10-28 Journal: Pharmaceuticals [Category] COVID-19, [키워드] acute respiratory syndrome analogue Antiviral agents antiviral drugs Cell Compound coronavirus cytotoxic effects docking energy transfer Enzymes evade evaluated fluorescent glycine High-throughput virtual screening homogeneous HTRF Human immune system inhibition activity inhibitor inhibitors majority methyltransferase mRNA MTase inhibitors nsp14 nsp16 performed S-adenosylmethionine SAM SARS-CoV-2 Screening Seven starting point target tested the SARS-CoV-2 Virtual screening [DOI] 10.3390/ph14121243 PMC 바로가기 [Article Type] Article
Structure–function analysis of the nsp14 N7–guanine methyltransferase reveals an essential role in Betacoronavirus replication423 Published on 2021-11-292022-10-29 Journal: Proceedings of the National Academy of Sciences of [Category] COVID-19, [키워드] acute respiratory syndrome affecting Analysis approach Betacoronavirus betacoronaviruses biochemical C-terminal catalytic cellular characterized conserved coronavirus CoV CoVs Critical defined Degradation develop domain drugs enzymatic activity enzyme Exoribonuclease feature genomes highlight host cell cytoplasm identify in silico in viral inhibitor innate immune involved MERS-CoV Messenger RNA methyltransferase Middle East MOST mRNA mRNA capping mRNAs Mutation N-terminal nonstructural protein 14 nsp14 nsp14-ExoN pandemic phenotype PROTECT replicate Replication replicative residue reveal RNA synthesis SARS-CoV SARS-CoV-2 selected Significance site-directed mutagenesis Substitution subunit syndrome targets tested translation unique viability Virological virus was used yielding [DOI] 10.1073/pnas.2108709118 PMC 바로가기 [Article Type] 423
Identification of fragments binding to SARS-CoV-2 nsp10 reveals ligand-binding sites in conserved interfaces between nsp10 and nsp14/nsp16 †Chemistry Published on 2021-10-062022-10-29 Journal: RSC Chemical Biology [Category] COVID-19, [키워드] activities affinity analogue applied binding complex conserved Coronavirus infections develop drug drug design enzyme exhibiting experiment Health identification identify increasingly inhibit Interaction Intervention less Ligand methyltransferase nsp14 Proofreading Protein Replication reveal RNA SARS-CoV-2 Small molecules starting point Vaccine viral replication were used X-ray crystallography [DOI] 10.1039/d1cb00135c PMC 바로가기 [Article Type] Chemistry
A Convenient and Biosafe Replicon with Accessory Genes of SARS-CoV-2 and Its Potential Application in Antiviral Drug DiscoverySARS-CoV-2의 보조 유전자와 항바이러스 약물 발견에 대한 잠재적 응용을 포함하는 편리하고 생물학적으로 안전한 복제품Article Published on 2021-10-012022-09-11 Journal: Virologica Sinica [Category] SARS, 치료제, [키워드] absence Accessory Analysis anti-SARS-CoV-2 antiviral drug Antiviral drug screening antiviral drugs application BAC Bacterial bacterial artificial chromosome benefit biosafety level Biosafety Level 3 BSL3 can be used cause cDNA Cell chromosome Containment costly Deletion disease drug effective drug effective drugs exonuclease expression genetics Genome genome replication Infection infectious inhibition assay Inhibition assays inhibitors Laboratory level 3 Live virus luciferase reporter gene methyltransferase Nsp12 nsp14 pandemic of COVID-19 pathogenicity Potential profile Replication replicon reporter gene Research research and development reverse genetics RNA RNA polymerase S protein SARS-CoV-2 sgRNA subgenomic RNA subgenomic RNAs this system transfection viral cDNA virion assembly virus wild-type virus [DOI] 10.1007/s12250-021-00385-9 PMC 바로가기 [Article Type] Article